Functional analysis of an Aspergillus ficuum phytase gene in Saccharomyces cerevisiae and its root-specific, secretory expression in transgenic soybean plants

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• 作者：Guilan Li ; Shaohui Yang ; Minggang Li ; Yake Qiao and Jiehua Wang
• 关键词：Aspergillus ficuum ; Phytase ; Root ; specific expression ; Secretory protein ; Transgenic soybean
• 刊名：Biotechnology Letters
• 出版年：2009
• 出版时间：August, 2009
• 年：2009
• 卷：31
• 期：8
• 页码：1297-1303
• 全文大小：313.0 KB

文摘

Phytases release inorganic phosphates from phytate in soil. A gene encoding phytase (AfPhyA) was isolated from Aspergillus ficuum and its ability to degrade phytase and release phosphate was demonstrated in Saccharomyces cerevisiae. A promoter from the Arabidopsis Pky10 gene and the carrot extensin signal peptide were used to drive the root-specific and secretory expression of the AfPhyA gene in soybean plants. The phytase activity and inorganic phosphate levels in transgenic soybean root secretions were 4.7 U/mg protein and 439 μM, respectively, compared to 0.8 U/mg protein and 120 μM, respectively, in control soybeans. Our results demonstrated the potential usefulness of the root-specific promoter for the exudation of recombinant phytases and offered a new perspective on the mobilization of phytate in soil to inorganic phosphates for plant uptake.