Analysis of the modification site in a small molecule-modified peptide by ion trap/time-of-flight hybrid mass spectrometry

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• 作者：Nariyasu Mano ; Mio Kamota ; Yusuke Inohana ; Shin-ichi Yamaguchi and Junichi Goto
• 关键词：Ion trap ; Mass spectometry ; Modification site ; MSn analysis ; Small molecule ; modified peptide ; Time ; of ; flight
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2006
• 出版时间：October, 2006
• 年：2006
• 卷：386
• 期：3
• 页码：682-688
• 全文大小：198 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Food Science
  Inorganic Chemistry
  Physical Chemistry
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1618-2650

文摘

Ion trap/time-of-flight hybrid mass spectrometers are powerful tools for the detailed structural analysis of modified peptides. We have analyzed Met-Lys-bradykinin modified with deoxycholate at the amino-terminus or the ε-amino group as model peptides. These two modified peptides produced fragment ions with the same nominal but different exact masses in tandem mass spectrometry with low-energy collision-induced dissociation. Accurate high-resolution analysis coupled with MS3 allowed us to distinguish the deoxycholate modification sites in the modified peptides.