Molecular Coordinated Regulation of Gene Expression During Ovarian Development in the Penaeid Shrimp

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• 作者：Ting Sze Lo ; Zhaoxia Cui ; Janice L.Y. Mong ; Queenie W.L. Wong ; Siu-Ming Chan ; Hoi Shan Kwan and Ka Hou Chu
• 关键词：gene expression ; Metapenaeus ensis ; ovarian development ; Penaeidae
• 刊名：Marine Biotechnology
• 出版年：2007
• 出版时间：August, 2007
• 年：2007
• 卷：9
• 期：4
• 页码：459-468
• 全文大小：466 KB
• 刊物类别：Earth and Environmental Science
• 刊物主题：Earth sciences
  Oceanography
  
• 出版者：Springer New York
• ISSN：1436-2236

文摘

To understand the molecular events of ovarian development in penaeid shrimp, RNA arbitrarily primed polymerase chain reaction (RAP-PCR) was used to identify differentially expressed genes during ovarian maturation in Metapenaeus ensis. From a screening of 700 clones in a cDNA library of the shrimp ovary by the products of RAP-PCR of different maturation stages, 91 fragments with differentially expressed pattern as revealed by dot-blot hybridization were isolated and sequenced. Forty-two of these fragments show significant sequence similarity to known gene products and the differentially expressed pattern of 10 putative genes were further characterized via Northern hybridization. Putative glyceraldehyde–3–phosphate dehydrogenase and arginine kinase are related to provision of energy for active cellular function in oocyte development. Translationally controlled tumor protein, actin, and keratin are related to the organization of cytoskeleton to accomplish growth and development of oocytes. High mobility group protein DSP1, heat shock protein 70, and nucleoside diphosphate kinase may act as repressors before the onset of ovarian maturation. Peptidyl-prolyl cis-trans isomerase and glutathione peroxidase are related to the stabilization of proteins and oocytes. This study provides new insights on the molecular events in the ovarian development in the shrimp.