Improving the discrimination capability of bioaerosols using a dual-channel ultraviolet aerodynamic particle sizer spectrometer

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• 作者：Xiao-Ling Ma (12)
  Wei-Xiong Zhao (3)
  Jia-Qiang Zhao (3)
  Na Li (3)
  Hai-Yang Zheng (3)
  Li Fang (3)
  Fei-Hu Chen (1) chenfeihuchina@126.com
  Wei-Jun Zhang (3) wjzhang@aiofm.ac.cn
• 关键词：Biological aerosols – ; Ultraviolet aerodynamic particle sizer spectrometer – ; Size distribution – ; Fluorescence – ; Dual ; channel
• 刊名：Aerobiologia
• 出版年：2012
• 出版时间：June 2012
• 年：2012
• 卷：28
• 期：2
• 页码：241-248
• 全文大小：607.6 KB
• 参考文献：1. Agranovski, V., & Ristovski, Z. D. (2005). Real-time monitoring of viable bioaerosols: capability of the UVAPS to predict the amount of individual microorganisms in aerosol particles. Journal of Aerosol Science, 36, 665–676.
  2. Agranovski, V., Ristovski, Z. D., Ayoko, G. A., & Morawska, L. (2004a). Performance evaluation of the UVAPS in measuring biological aerosols: Fluorescence spectra from NAD(P)H coenzymes and riboflavin. Aerosol Science and Technology, 38, 354–364.
  3. Agranovski, V., Ristovski, Z., Blackall, P. J., & Morawska, L. (2004b). Size-selective assessment of airborne particles in swine confinement building with the UVAPS. Atmospheric Environment, 38, 3893–3901.
  4. Agranovski, V., Ristovski, Z., Hargreaves, M., Blackall, P. J., & Morawska, L. (2003a). Real-time measurement of bacterial aerosols with the UVAPS: Performance evaluation. Journal of Aerosol Science, 34, 301–317.
  5. Agranovski, V., Ristovski, Z., Hargreaves, M., Blackall, P. J., & Morawska, L. (2003b). Performance evaluation of the UVAPS: Influence of physiological age of airborne bacteria and bacterial stress. Journal of Aerosol Science, 34, 1711–1727.
  6. Brosseau, L. M., Vesley, D., Rice, N., Goodell, K., Nellis, M., & Hairston, P. (2000). Differences in detected fluorescence among several bacterial species measured with a direct-reading particle sizer and fluorescence detector. Aerosol Science and Technology, 32, 545–558.
  7. Courvoisier, F., Boutou, V., Guyon, L., Roth, M., Rabitz, H., & Wolf, J.-P. (2006). Discriminating bacteria from other atmospheric particles using femtosecond molecular dynamics. Journal of Photochemistry and Photobiology. A, Chemistry, 180, 300–306.
  8. Hairston, P. P., Ho, J., & Quant, F. R. (1997). Design of an instrument for real-time detection of bioaerosols using simultaneous measurement of particle aerodynamic size and intrinsic fluorescence. Journal of Aerosol Science, 28, 471–482.
  9. Huffman, J. A., Treutlein, B., & P枚schl, U. (2010). Fluorescent biological aerosol particle concentrations and size distributions measured with an Ultraviolet Aerodynamic Particle Sizer (UV-APS) in Central Europe. Atmospheric Chemistry and Physics, 10, 3215–3233.
  10. Kanaani, H., Hargreaves, M., Ristovski, Z., & Morawska, L. (2007). Performance assessment of UVAPS: Influence of fungal spore age and air exposure. Journal of Aerosol Science, 38, 83–96.
  11. Kanaani, H., Hargreaves, M., Ristovski, Z., & Morawska, L. (2008a). Deposition rates of fungal spores in indoor environments, factors effecting them and comparison with non-biological aerosols. Atmospheric Environment, 42, 7141–7154.
  12. Kanaani, H., Hargreaves, M., Smith, J., Ristovski, Z., Agranovski, V., & Morawska, L. (2008b). Performance of UVAPS with respect to detection of airborne fungi. Journal of Aerosol Science, 39, 175–189.
  13. La Flamme, C., Verreault, D., Lavigne, S., Trudel, L., Ho, J., & Duchaine, C. (2005). Autofluorescence as a viability marker for detection of bacterial spores. Frontiers in Bioscience, 10, 1647–1653.
• 作者单位：1. School of pharmacy, Anhui Medical University, 81 Meishan Road, Hefei, Anhui, China2. Department of Clinical Laboratory, Anhui Province Hospital, 9 Lujiang Road, Hefei, Anhui, China3. Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, 350 Shushanhu Road, Hefei, Anhui, China
• 刊物类别：Earth and Environmental Science
• 刊物主题：Environment
  Environment
  Atmospheric Protection, Air Quality Control and Air Pollution
  Plant Pathology
  Allergology
  Pneumology and Respiratory System
  Environmental Biotechnology
  
• 出版者：Springer Netherlands
• ISSN：1573-3025

文摘

We report on the results of laboratory evaluation of a dual-channel ultraviolet aerodynamic particle sizer spectrometer for real-time, continues monitoring of bio-aerosols. The detected fluorescence light was divided into two channels, simultaneously measuring the blue (400–500 nm) and green (500–600 nm) signal, which were corresponding to the fluorescence spectra of NAD(P)H and riboflavin, respectively. Primary results show the different fluorescence properties of the bacteria aerosols (Escherichia coli, Staphylococci and Bacillus subtilis var. niger spore) and the substrate (TSA eluate solutions, TSB and Nutrient broth). We demonstrated a promising method to improve the discrimination capability of bioaerosols from non-bioaerosol background components and to distinguish different types of bioaerosol particles.