The Application of CdTe/CdS in the Detection of Carcinoembryonic Antigen by Fluorescence Polarization Immunoassay

详细信息    查看全文

• 作者：Jianniao Tian (1) tianjn58@yahoo.com.cn
  Liujin Zhou (1)
  Yanchun Zhao (1)
  Yuan Wang (1)
  Yan Peng (12)
  Xue Hong (12)
  Shulin Zhao (1)
• 关键词：Fuorescence polarization immunoassay – ; Core– ; shell quantum dots – ; Carcinoembryonic antigen – ; Cell imaging
• 刊名：Journal of Fluorescence
• 出版年：2012
• 出版时间：November 2012
• 年：2012
• 卷：22
• 期：6
• 页码：1571-1579
• 全文大小：471.5 KB
• 参考文献：1. Gold P, Shuster J, Freedman SO (1978) Carcinoembryonic antigen(CEA) in clinicalmedicime. Cancer 42:1399–1405
  2. Goldenberg DM, Sharkey RM, Primus FJ (1976) Carcinoembryonic antigen in hist opathology: immunoperoxidase staining of conventional tissue sections. Natl Cancer Inst 57:11–22
  3. Tang DP, Yuan R, Chai YQ (2006) Magnetic core shell Fe3O4@Ag nanoparticles coated carbon paste interface for studies of carcinoembryonic antigen in clinical immunoassay. J Phys Chem B 110:11640–11646
  4. Laboria N, Fragoso A, Kemmner W, Latta D, Nilsson O, Botero ML, Drese K, O’Sullivan CK (2010) Amperometric immunosensor for carcinoembryonic antigen in colon cancer samples based on monolayers of dendritic bipodal scaffolds. Anal Chem 82:1712–1719
  5. Molina R, Judith J, Filella X, Zanon G, Pahisa J, Munoz M, Farrus B, Latre ML, Escrichc C, Estape J, Ballesta AM (1998) C-erbB-2 oncoprotein, CEA, and CA15.3 in patients with breast cancer: prognostic value. Breast Cancer Res Treat 51:109–119
  6. Mujagic Z, Mujagic H, Prnjavorac B (2004) The relationship between circulating carcinoembryonic antigen (CEA) levels and parameters of primary tumor and metastases in breast cancer patients. Med Arh 58:23–26
  7. Theriault RL, Hortobagyi GN, Fritsche HA, Frye D, Martinez R, Buzdar AU (1989) The role of serum CEA as a prognostic indicator in stage II and III breast cancer patients treated with adjuvant chemotherapy. Cancer 63:828–835
  8. Macdonald JS (1999) Carcinoembryonic antigen screening: pros and cons. Semin Oncol 26:556–560
  9. Dnistrian AM, Schwartz MK, Greenberg EJ, Smith CA, Schwartz DC (1991) CA15-3 and carcinoembryonic antigen in the clinical evaluation of breast cancer. Clin Chim Acta 200:81
  10. Yang HJ, Jiang SJ, Yang YJ, Hwang CJ (1995) Speciation of tin by reversed phase liquid chromatography with inductively coupled plasma mass spectrometric detection. Anal Chim Acta 312:141–148
  11. Chon H, Lee S, Son SW, Oh CH, Choo J (2009) Highly sensitive immunoassay of lung cancer marker carcinoembryonic antigen using surface-enhanced raman scattering of hollow gold nanospheres. Anal Chem 81:3029–3034
  12. Tang DP, Ren JJ (2008) In situ amplified electrochemical immunoassay for carcinoembryonic antigen using horseradish peroxidase-encapsulated nanogold hollow microspheres as labels. Anal Chem 80:8064–8070
  13. Shiku H, Matsue T, Uchida I (1996) Detection of microspotted carcinoembryonic antigen on a glass substrate by scanning electrochemical microscopy. Anal Chem 68:1276–1278
  14. Yuan JL, Wang GL, Majima K, Matsumoto K (2001) Synthesis of a terbium fluorescent chelate and its application to time-resolved fluoroimmunoassay. Anal Chem 73:1869–1876
  15. Shim WB, Kolosova AY, Kim YJ, Yang ZY, Park SJ, Eremin SA, Lee IS, Chung DH (2004) Fluorescence polarization immunoassay based on a monoclonal antibody for the detection of ochratoxin A. Int J Food Sci Tech 39:829–837
  16. Latychevskaiab TY, Renna A, Wild UP (2006) A single-molecule study of polycrystalline microstructure by fluorescence polarization spectroscopy. J Lum 118:111–122
  17. Eremin SA, Murtazina NR, Ermolenko DN, Zherdev AV, Mart’ianov AA, Yazynina EV, Michura IV, Formanovsky AA, Dzantiev BB (2005) Production of polyclonal antibodies and development of fluorescence polarization immunoassay for sulfanilamide. Anal Lett 38:951–969
  18. Murtazina NR, Eremin SA, Mozoleva OV, Everest SJ, Brown AJ, Jackman R (2004) Fluorescent polarization immunoassay for sulphadiazine using a high specificity antibody. Int J Food Sci Tech 39:879–889
  19. Gao XH, Cui YY, Levenson RM, Chung LWK, Nie SM (2004) In vivo cancer targeting and imaging with semiconductor quantum dots. Nat Biotech 22:969–976
  20. Chen W, Peng CF, Jin ZY, Qiao RR, Wang WY, Zhu SF, Wang LB, Jing QH, Xu CL (2009) Ultrasensitive immunoassay of 7-aminoclonazepam in human urine based on CdTe nanoparticle bioconjugations by fabricated microfluidic chip. Biosens Bioelectron 16:2051–2056
  21. Warner MG, Grate JW, Tyler A, Ozanich RM, Miller KD, Lou JL, Marks JD, Bruckner-Le CJ (2009) Quantum dot immunoassays in renewable surface column and 96-well plate formats for the fluorescence detection of botulinum neurotoxin using high-affinity antibodies. Biosens Bioelectron 25:179–184
  22. Tian JN, Liu RJ, Zhao YC, Peng Y, Hong X, Xu Q, Zhao SL (2010) Synthesis of CdTe/CdS/ZnS quantum dots and their application in imaging of hepatocellular carcinoma cells and immunoassay for alpha fetoprotein. Nanotechnology 21:305101–305108
  23. Tian JN, Liu RJ, Zhao YC, Xu Q, Zhao SL (2009) Controllable synthesis and cell-imaging studies on CdTe quantum dots together capped by glutathione and thioglycolic acid. J Colloid Interf Sci 336:504–509
  24. Fery-Forgue S, Lavabre D (1999) Are fluorescence quantum yields to tricky to measure? A demonstration using familiar stationary products. J Chem Ed 76:1260–1264
  25. 脰nnerfjord P, Eremin S, Emn茅us J, Marko-Varga G (1998) Fluorescence polarisation for immunoreagent characterization. J Immunol Methods 213:31–39
  26. Zhan HW, Lin LC, Wei MS, Su XZ, Jian ZS (2010) Fluorescence polarization immunoassay for salinomycin based on monoclonal antibodies. Sci Chin Chem 53:553–555
  27. Ekins R, Edwards P (1997) On the meaning of ‘sensitivity’. Clin Chem 43:1824–1837
  28. Pourfarzaneh M, White GW, Landon J, Smith DS (1980) Cortisol directly determined in serum by fluoroimmunoassay with magnetizable solid phase. Clin Chem 26:730–733
  29. Niculescu F, Rus HG, Ionescu D, Maican A, Petcovici M, Malide D, Rahaian R (1989) Isolation of anti-carcinoembryonic antigen(CEA), specific immunoglobulins and their use in immunecytochemical and enzyme-immunoassays(ELISA). Arch Roum Pathol Exp Microbiol 72:47–52
  30. Derfus AM, Chan WCW, Bhatia SN (2004) Intracellular delivery of quantum dots for live cell labeling and organelle tracking. Adv Mat 16:961–966
  31. Xie MX, Liu HH, Chen P, Zhang ZL, Wang XH, Xie ZX, Du YM, Pan BQ, Pang DW (2005) CdSe/ZnS-labeled carboxymethyl chitosan as a bioprobe for live cell imaging. Chem Commun 5518–5520
  32. Jaiswal JK, Mattoussi H, Mauro JM, Simon SM (2003) Long-term multiple color imaging of live cells using quantum dot bioconjugates. Nat Biotechnol 21:47–51
  33. Voura EB, Jaiswal JK, Mattoussi H, Simon SM (2004) Tracking metastatic tumor cell extravasation with quantum dot nanocrystals and fluorescence emission-scanning microscopy. Nat Med 10:993–998
  34. Chan WC, Nie SM (1998) Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 281:2016–2018
  35. Chen FQ, Gerion D (2004) Fluorescent CdSe/ZnS nanocrystal-peptide conjugates for long-term, nontoxic imaging and nuclear targeting in living cells. Nano Lett 4:1827–1832
  36. Haiqiang Y, Edwin L, Stephen WY, Jian L, Hui T, Ping C (2006) Quantitative analysis of free fatty acids in rat plasma using matrix-assisted laser desorption/ionization time-of-Xight mass spectrometry with meso-tetrakis porphyrin as matrix. Anal Biochem 354:182–191
  37. Chen G, Desen W, Hou JH, Lin SX, Pan ZZ, Zhou ZW, Chen YB (2001) Tissue CEA and its clinicobiologieal significance in patients with colorectral cancer. Chin J Cancer 20:628–630
• 作者单位：1. Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering of Guangxi Normal University, Guilin, 541004 China2. College of Life Science, Guangxi Normal University, Guilin, 541004 China
• ISSN：1573-4994

文摘

A novel and portable strategy based on fluorescence polarization immunoassay (FPIA) using quantum dots (QDs) was described in this study for simple, rapid, and sensitive detection of carcinoembryonic antigen (CEA). Under optimal conditions, the sensor has a wide dynamic range (from 0.5 ng/mL to 200 ng/mL) and a good correlation. The limit of detection (LOD) is 0.21 ng/mL (S/N = 3). The sensor has been applied for detection of carcinoembryonic antigen in 10 human serum samples with the range of recovery from 92.1 % to 103.6 %. Furthermore, bioconjugation of the core–shell QDs with streptavidin (SA) has been successfully applied in immunofluorescent imaging of the human hepatocellular carcinoma (HEPG2) cell line. The experimental results demonstrated the successful application of QDs-based fluorescence polarization immunoassay for detection of target proteins of biomedical importance. This strategy shows great promise for clinical diagnoses and basic discovery with high sensitivity, good specificity, simple procedures and short analysis time.