Estradiol promotes neural stem cell differentiation into endothelial lineage and angiogenesis in injured peripheral nerve

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• 作者：Haruki Sekiguchi (1) (3) (8)
  Masaaki Ii (2)
  Kentaro Jujo (1) (4)
  Tina Thorne (1)
  Aiko Ito (1)
  Ekaterina Klyachko (1)
  Hiromichi Hamada (5)
  John A. Kessler (6)
  Yasuhiko Tabata (7)
  Masatoshi Kawana (3) (4)
  Michio Asahi (2)
  Nobuhisa Hagiwara (4)
  Douglas W. Losordo (1)
  
• 关键词：Estrogen ; Neural stem cell ; Cell transplantation ; Angiogenesis ; Endothelial differentiation ; Nervous system
• 刊名：Angiogenesis
• 出版年：2013
• 出版时间：January 2013
• 年：2013
• 卷：16
• 期：1
• 页码：45-58
• 全文大小：937KB
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• 作者单位：Haruki Sekiguchi (1) (3) (8)
  Masaaki Ii (2)
  Kentaro Jujo (1) (4)
  Tina Thorne (1)
  Aiko Ito (1)
  Ekaterina Klyachko (1)
  Hiromichi Hamada (5)
  John A. Kessler (6)
  Yasuhiko Tabata (7)
  Masatoshi Kawana (3) (4)
  Michio Asahi (2)
  Nobuhisa Hagiwara (4)
  Douglas W. Losordo (1)
  
  1. Feinberg Cardiovascular Research Institute, Northwestern University Feinberg School of Medicine, Chicago, IL, USA
  3. Department of Cardiology, Aoyama Hospital, Tokyo Women鈥檚 Medical University, Tokyo, Japan
  8. Department of Cardiology, National Hospital Organization Yokohama Medical Center, Kanagawa, Japan
  2. Department of Pharmacology, Faculty of Medicine, Osaka Medical College, 2-7, Daigaku-machi, Takatsuki, 569-8686, Osaka, Japan
  4. Department of Cardiology, Tokyo Women鈥檚 Medical University, Tokyo, Japan
  5. Departments of Pediatrics and Pediatric Intensive Care Medicine, Yachiyo Medical Center, Tokyo Women鈥檚 Medical University, Chiba, Japan
  6. Department of Neurology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA
  7. Department of Biomaterials, Field of Tissue Engineering, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
  
• ISSN：1573-7209

文摘

Neural stem cells (NSCs) differentiate into endothelial cells (ECs) and neuronal cells. Estradiol (E2) is known to exhibit proangiogenic effects on ischemic tissues via EC activation. Therefore, we hypothesized that E2 can promote the therapeutic potential of NSC transplantation for injured nerve repair via the differentiation of NSCs into ECs during neovascularization. NSCs isolated from newborn mouse brains were transplanted into injured sciatic nerves with (NSC/E2 group) or without E2-conjugated gelatin hydrogel (E2 group). The NSC/E2 group exhibited the greatest recovery in motor nerve conduction velocity, voltage amplitude, and exercise tolerance. Histological analyses revealed increased intraneural vascularity and blood perfusion as well as striking NSC recruitment to the neovasculature in the injured nerves in the NSC/E2 group. In vitro, E2 enhanced the NSC migration and proliferation inhibiting apoptosis. Fluorescence-activated cell sorting analysis also revealed that E2 significantly increased the percentage of CD31 in NSCs, and the effect of E2 was completely neutralized by the estrogen receptor antagonist ICI. The combination of E2 administration and NSC transplantation cooperatively improved the functional recovery of injured peripheral nerves, at least in part, via E2-associated NSC differentiation into ECs. These findings provide a novel mechanistic insight into both NSC biology and the biological effects of endogenous E2.