Molecular character of a phosphatase 2C (PP2C) gene relation to stress tolerance in Arabidopsis thaliana

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• 作者：Jihong Zhang (1) (2)
  Xiushan Li (1)
  Zhimin He (1)
  Xiaoying Zhao (1)
  Qiming Wang (1)
  Bo Zhou (1)
  Dashi Yu (1)
  Xinqun Huang (1)
  Dongying Tang (1)
  Xinhong Guo (1)
  Xuanming Liu (1)
  
• 关键词：Loss ; of ; function mutant ; GFP ; GUS ; Stress tolerance ; Water ; loss assays ; Seed germination
• 刊名：Molecular Biology Reports
• 出版年：2013
• 出版时间：March 2013
• 年：2013
• 卷：40
• 期：3
• 页码：2633-2644
• 全文大小：1324KB
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• 作者单位：Jihong Zhang (1) (2)
  Xiushan Li (1)
  Zhimin He (1)
  Xiaoying Zhao (1)
  Qiming Wang (1)
  Bo Zhou (1)
  Dashi Yu (1)
  Xinqun Huang (1)
  Dongying Tang (1)
  Xinhong Guo (1)
  Xuanming Liu (1)
  
  1. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha, 410082, China
  2. Department of Biology Engineering, Xiangtan University, Xiangtan, 411105, China
  
• ISSN：1573-4978

文摘

Protein phosphatases type 2C (PP2Cs) from group A, which includes the ABI1/HAB1 and PP2CA branches, are key negative regulators of ABA signaling. HAI-1 gene had been shown to affect both seed and vegetative responses to ABA, which is one of PP2Cs clade A in Arabidopsis thaliana. Transgenic plants containing pHAI-1::GUS (β-glucuronidase) displayed GUS activity existing in the vascular system of leave veins, stems and petioles. Green fluorescent protein fused HAI-1 (HAI-1-GFP) was found in the nucleus through transient transformation assays with onion epidermal cells. The water-loss assays indicated the loss-of-function mutants did not show symptoms of wilting and they had still turgid green rosette leaves. The assays of seed germination by exogenous ABA and NaCl manifested that the loss-of-function mutants displayed higher insensitivity than wild-type plants. Taken together, the final results suggest that the HAI-1 (AT5G59220) encoded a nuclear protein and it can be highly induced by ABA and wound in Arabidposis, the stress-tolerance phenotype showed a slightly improvement when HAI-1 gene was disrupted.