First report of a 16SrIII-B subgroup phytoplasma associated with leaf reddening, virescence and phyllody of purple coneflower

详细信息    查看全文

• 作者：Jana Fránová (1)
  Josef ?pak (1)
  Marie ?imková (1)
  
• 关键词：Echinacea purpurea ; PCR/RFLP ; 16S ; 23S rRNA gene ; rpl15 gene ; SecY gene ; Sequencing
• 刊名：European Journal of Plant Pathology
• 出版年：2013
• 出版时间：May 2013
• 年：2013
• 卷：136
• 期：1
• 页码：7-12
• 全文大小：351KB
• 参考文献：1. Bertaccini, A., Paltrinieri, S., Contaldo, N., Duduk, B., Nahdi, S., Benni, A., et al. (2009). Different phytoplasmas infecting purple coneflower in Italy. / Journal of Plant Pathology, 91, 49.
  2. Chiykowski, L. N. (1981). Association of mycoplasma-like organisms with clover yellow edge disease. / Canadian Journal of Plant Pathology, 3, 139-44. CrossRef
  3. Deng, S., & Hiruki, C. (1991). Amplification of 16S rRNA genes from culturable and non-culturable mollicute. / Journal of Microbiological Methods, 14, 53-1. CrossRef
  4. Firrao, G., Carraro, L., Gobbi, E., & Locci, R. (1996). Molecular characterization of phytoplasmas causing phyllody in clover and other herbaceous host in Northern Italy. / European Journal of Plant Pathology, 102, 817-22. CrossRef
  5. Fránová, J., Paltrinieri, S., Botti, S., ?imková, M., & Bertaccini, A. (2004). Association of phytoplasmas and viruses with malformed clovers. / Folia Microbiologica, 49, 617-24. CrossRef
  6. Fránová, J., P?ibylová, J., & Petrzik, K. (2009a). Purple coneflower with reddening and phyllody: a new host of clover phyllody phytoplasma. / European Journal of Plant Pathology, 123, 85-0. CrossRef
  7. Fránová, J., Navrátil, M., & Jake?ová, H. (2009b). Molecular identification of stolbur phytoplasma associated with red clover dwarf disease symptoms. / Journal of Phytopathology, 157, 502-06. CrossRef
  8. Gibb, K. S., Padovan, A. C., & Mogen, B. D. (1995). Studies on sweet potato little-leaf phytoplasma detected in sweet potato and other plant species growing in northern Australia. / Phytopathology, 85, 169-74. CrossRef
  9. Gundersen, D. E., & Lee, I.-M. (1996). Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer pairs. / Phytopathologia Mediterranea, 35, 144-51.
  10. Hwang, S. F., Chang, K. F., Howard, R. J., Khadhair, A. H., Gaudiel, R. G., & Hiruki, C. (1997). First report of yellows phytoplasma disease in purple coneflower ( / Echinacea spp.) in Canada. / Journal of Plant Diseases and Protection, 104, 182-92.
  11. Khadhair, A. H., Hwang, S. F., Chang, K. F., & Howard, R. J. (1997). Molecular identification of aster yellows phytoplasma in purple coneflower and monarda based on PCR amplification and RFLP analyses of 16S rDNA sequences. / Journal of Plant Diseases and Protection, 104, 403-10.
  12. Lee, I.-M., Hammond, R. W., Davis, R. E., & Gundersen, D. E. (1993). Universal amplification and analysis of pathogen 16S rDNA for classification and identification of mycoplasmalike organisms. / Phytopathology, 83, 834-42. CrossRef
  13. Lee, I.-M., Gundersen, D. E., Hammond, R. W., & Davis, R. E. (1994). Use of mycoplasmalike organism (MLO) group-specific oligonucleotide primers for nested-PCR assays to detect mixed-MLO infections in a single host plant. / Phytopathology, 84, 559-66. CrossRef
  14. Lee, I.-M., Bertaccini, A., Vibio, M., & Gundersen, D. E. (1995). Detection of multiple phytoplasmas in perennial fruit trees with decline symptoms in Italy. / Phytopathology, 85, 728-35. CrossRef
  15. Lee, I.-M., Gundersen, D. E., Davis, R. E., & Bartoszyk, I. M. (1998). Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA and ribosomal protein gene sequences. / International Journal of Systematic Bacteriology, 48, 1153-169. CrossRef
  16. Lee, I.-M., Bottner, K. D., Dally, E. L., & Davis, R. E. (2008). First report of purple coneflower phyllody associated with a 16Sr I-B phytoplasma in Marryland. / Plant Disease, 92, 654. CrossRef
  17. Lee, I.-M., Bottner-Parker, K. D., Zhao, Y., Davis, R. E., & Harrison, N. A. (2010). Phylogenetic analysis and delineation of phytoplasmas based on / secY gene sequences. / International Journal of Systematic and Evolutionary Microbiology, 60, 2887-897. CrossRef
  18. Lorenz, K. H., Schneider, B., Ahrens, U., & Seem?ller, E. (1995). Detection of apple proliferation and pear decline phytoplasmas by PCR amplification of ribosomal and nonribosomal DNA. / Phytopathology, 85, 771-76. CrossRef
  19. Pavlovic, S., Starovic, M., Stojanovic, S., Popovic, T., Aleksic, G., Drazic, S., et al. (2011). / Echinacea purpurea- a host of 16SrXII-A phytoplasma group in Serbia. / Phytopathogenic Mollicutes, 1, 35-9. CrossRef
  20. Pearce, T. L., Scott, J. B., & Pethybridge, S. J. (2011). First report of a 16SrII-D subgroup phytoplasma associated with pale purple coneflower witches-broom disease in Australia. / Plant Disease, 95, 773. CrossRef
  21. Radisek, S., Ferant, N., Jakse, J., & Javornik, B. (2008). Identification of a phytoplasma from the aster yellows group infecting purple coneflower ( / Echinacea purpurea) in Slovenia. / New Disease Reports, 18, 13.
  22. ?afá?ová, D., Válová, P., Flidr, P., & Navrátil, M. (2011). Molecular identification of 16SrIII and 16SrXII phytoplasma groups in / Chenopodium album in the Czech Republic. / Bulletion of Insectology, 64, S85–S86.
  23. Schneider, B., Seemüller, E., Smart, C. D., & Kirkpatrick, B. C. (1995). Phylogenetic classification of plant pathogenic mycoplasmalike organisms or phytoplasmas. In S. Razin & J. G. Tully (Eds.), / Molecular and diagnostic procedures in mycoplasmology (pp. 369-80). New York: Academic Press Inc. CrossRef
  24. Staniulis, J. B., Davis, R. E., Jomantiene, R., Kalvelyte, A., & Dally, E. L. (2000). Single and mixed phytoplasma infections in phyllody- and dwarf-diseased clover plants in Lithuania. / Plant Disease, 84, 1061-066. CrossRef
  25. Stanosz, G. R., Heimann, M. F., & Lee, I.-M. (1997). Purple coneflower is a host of the aster yellows phytoplasma. / Plant Disease, 81, 424. CrossRef
  26. Tseng, Y.-W., Deng, W.-L., Chang, C.-J., Su, C.-C., Chen, C.-L., & Jan, F. J. (2012). First report of a 16SrII-A subgroup phytoplasma associated with purple coneflower ( / Echinacea purpurea) witches-broom disease in Taiwan. / Plant Disease, 96, 582. CrossRef
  27. Wang, K., & Hiruki, C. (2001). Use of heteroduplex mobility assay for identification and differentiation of phytoplasmas in the aster yellows group and the clover proliferation group. / Phytopathology, 91, 546-52. CrossRef
  28. Wang, K., & Hiruki, C. (2005). Distinctions between phytoplasmas at the subgroup level detected by heteroduplex mobility assay. / Plant Pathology, 54(5), 625-33. CrossRef
  
• 作者单位：Jana Fránová (1)
  Josef ?pak (1)
  Marie ?imková (1)
  
  1. Department of Plant Virology, Biology Centre of the ASCR, v.v.i., Institute of Plant Molecular Biology, Brani?ovská 31, 370 05, ?eské Budějovice, Czech Republic
  
• ISSN：1573-8469

文摘

Purple coneflower plants showing leaf reddening and flower abnormalities were observed in South Bohemia (Czech Republic). Transmission electron microscopy observations showed phytoplasmas in sieve cells of symptomatic plants but not in healthy ones. Polymerase chain reactions with universal and group specific phytoplasma primers followed by restriction fragment length polymorphism analyses of 16S rDNA allowed us to classify the detected phytoplasmas into the X-disease group, ribosomal subgroup 16SrIII-B. Sequence analyses of the 16S-23S ribosomal operon (1684?bp), ribosomal protein L15, and protein translocase genes (1566?bp) confirmed the closest relationship with phytoplasmas belonging to the 16SrIII ribosomal group, specifically the 16SrIII-B subgroup. The current study reports purple coneflower as a new host for the X-disease phytoplasma group in the Czech Republic and worldwide.