The ecological roles of bacterial populations in the surface sediments of coastal lagoon environments in Japan as revealed by quantification and qualification of 16S rDNA

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• 作者：Shun Tsuboi (1) (2)
  Takashi Amemiya (1)
  Koji Seto (3)
  Kiminori Itoh (1)
  Narasimmalu Rajendran (1) (3) (4)
  
• 关键词：Coastal lagoon environments ; Lakes Shinji and Nakaumi ; Surface sediments ; Bacterial 16S rDNA ; Stable carbon and nitrogen isotope ratio ; Ecological roles of sedimentary bacteria
• 刊名：World Journal of Microbiology and Biotechnology
• 出版年：2013
• 出版时间：May 2013
• 年：2013
• 卷：29
• 期：5
• 页码：759-774
• 全文大小：815KB
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• 作者单位：Shun Tsuboi (1) (2)
  Takashi Amemiya (1)
  Koji Seto (3)
  Kiminori Itoh (1)
  Narasimmalu Rajendran (1) (3) (4)
  
  1. Graduate School of Environment and Information Sciences, Yokohama National University, Yokohama, 240-8501, Japan
  2. National Institute for Environmental Studies, Tsukuba, 305-8506, Japan
  3. Research Center for Coastal Lagoon Environments, Shimane University, Matsue, 690-8504, Japan
  4. School of Bio-sciences and Technology, VIT University, Vellore, 632014, India
  
• ISSN：1573-0972

文摘

Based on quantification and qualification of bacterial 16S rDNA, we verified the bacterial ecological characteristics of surface sediments of Lakes Shinji and Nakaumi, which are representative of coastal lagoons in Japan. Quantification and qualification of the 16S rDNA sequences was carried out using real time polymerase chain reaction and polymerase chain reaction denaturing gradient gel electrophoresis and non-metric multidimensional scaling, respectively. The results revealed that the copy number per gram of sediment ranged from 8.33?×?108 (Lake Nakaumi) to 1.69?×?1011 (Honjo area), suggesting that bacterial carbon contributed only 0.05-.64?% of the total carbon content in the samples. Compared with other aquatic environments, these results indicate that sedimentary bacteria are not likely to be important transporters of nutrients to higher trophic levels, or to act as carbon sinks in the lagoons. The bacterial compositions of Lake Shinji and Lake Nakaumi and the Honjo area were primarily influenced by sediment grain sizes and salinity, respectively. Statistical comparisons of the environmental properties suggested that the areas that were oxygen-abundant (Lake Shinji) and at a higher temperature (Honjo area) presented efficient organic matter degradation. The 16S rDNA copy number per gram of carbon and nitrogen showed the same tendency. Consequently, the primary roles of bacteria were degradation and preservation of organic materials, and this was affected by oxygen and temperature. These roles were supported by the bacterial diversity rather than the differences in the community compositions of the sedimentary bacteria in these coastal lagoons.