Vector integration in triple R gene transformants and the clustered inheritance of resistance against potato late blight

详细信息    查看全文

• 作者：Suxian Zhu (1)
  Anita Duwal (1)
  Qi Su (1)
  Jack H. Vossen (1)
  Richard G. F. Visser (1)
  Evert Jacobsen (1)
  
• 关键词：Potato late blight ; R gene stacking ; Vector integration ; Inheritance
• 刊名：Transgenic Research
• 出版年：2013
• 出版时间：April 2013
• 年：2013
• 卷：22
• 期：2
• 页码：315-325
• 全文大小：365KB
• 参考文献：1. Abdal-Aziz SA, Pliego-Alfaro F, Quesada MA, Mercado JA (2006) Evidence of frequent integration of non-T-DNA vector backbone sequences in transgenic strawberry plant. J Biosci Bioeng 101(6):508-10 CrossRef
  2. Afolabi AS, Worland B, Snape JW, Vain P (2004) A large-scale study of rice plants transformed with different T-DNAs provides new insights into locus composition and T-DNA linkage configurations. Theor Appl Genet 109(4):815-26 CrossRef
  3. BASF (2010) European Commission approves Amflora starch potato. Available at: http://www.basf.com/group/pressrelease/P-10-179. Accessed 2 Mar 2012
  4. Basri Z (2005) Transgene inheritance in transgenic wheat. J Matermatika Sains 10:1-
  5. Cannell ME, Doherty A, Lazzeri PA, Barcelo P (1999) A population of wheat and tritordeum transformants showing a high degree of marker gene stability and heritability. Theor Appl Genet 99(5):772-84 CrossRef
  6. Cao J, Zhao JZ, Tang JD, Shelton AM, Earle ED (2002) Broccoli plants with pyramided cry1Ac and cry1C Bt genes control diamondback moths resistant to Cry1A and Cry1C proteins. Theor Appl Genet 105(2-):258-64
  7. De Buck S, De Wilde C, Van Montagu M, Depicker A (2000) T-DNA vector backbone sequences are frequently integrated into the genome of transgenic plants obtained by / Agrobacterium-mediated transformation. Mol Breed 6(5):459-68 CrossRef
  8. Deineko EV, Novoselya TV, Zagorskaya AA, Filipenko EV, Shumnyi VK (1999) Expression and inheritance of kanamycin resistance in a large number of transgenic tobacco generated by / Agrobacterium-mediated transformation. Dokl Akad Nauk SSSR 369(3):420-23
  9. European Commission (2001) Directive 2001/18/EC of the European parliament and of the council of 12 March 2001 on the deliberate release into the environment of genetically modified organisms and repealing. Available at: http://www.wipo.int/wipolex/en/details.jsp?id=10335. Accessed 15 Jan 2012
  10. European Food Safety Authority (EFSA) (2012) Scientific opinion on the annual post-market environmental monitoring (PMEM) report from BASF plant science company GmbH on the cultivation of genetically modified potato. Available at: http://www.efsa.europa.eu/en/efsajournal/pub/2558.htm. Accessed 8 Feb 2012
  11. Fedoroff N, Banks JA, Masson P (1989) Molecular genetic-analysis of the maize suppressor-mutator elements epigenetic developmental regulatory mechanism. Genome 31(2):973-79 CrossRef
  12. Field DL, Pickup M, Barrett SCH (2012) The influence of pollination intensity on fertilization success, progeny sex ratio, and fitness in a wind-pollinated, dioecious plant. Int J Plant Sci 173(2):184-91 CrossRef
  13. Gahakwa D, Maqbool SB, Fu X, Sudhakar D, Christou P, Kohli A (2000) Transgenic rice as a system to study the stability of transgene expression: multiple heterologous transgenes show similar behaviour in diverse genetic backgrounds. Theor Appl Genet 101(3):388-99 CrossRef
  14. Gelvin SB (2003) / Agobacterium-mediated plant transformation: the biology behind the “gene-jockeying-tool. Microbiol Mol Biol Rev 67(1):16-7 CrossRef
  15. Halpin C (2005) Gene stacking in transgenic plants—the challenge for 21st century plant biotechnology. Plant Biotechnol J 3:141-55 CrossRef
  16. Herrera-Estrella L, De Block M, Messens E, Hernalsteens JP, Van Montagu M, Schell J (1983) Chimeric genes as dominant selectable markers in plant cells. EMBO J 2(6):987-95
  17. International Service for the Acquisition of Agri-biotech Applications (ISAAA) (2012) Global status of commercialized biotech/GM crops: 2011. Available at: http://www.isaaa.org/resources/publications/briefs/43/executivesummary/default.asp. Accessed 21 Jan 2012
  18. Jaccaud E, Hohne M, Meyer R (2003) Assessment of screening methods for the identification of genetically modified potatoes in raw materials and finished products. J Agric Food Chem 51(3):550-57 CrossRef
  19. Kelly BA, Kado CI (2002) / Agrobacterium-mediated T-DNA transfer and integration into the chromosome of / Streptomyces lividans. Mol Plant Pathol 3(3):125-34 CrossRef
  20. Kim HJ, Lee HR, Jo KR, Mortazavian SM, Huigen DJ, Evenhuis B, Kessel G, Visser RG, Jacobsen E, Vossen JH (2012) Broad spectrum late blight resistance in potato differential set plants MaR8 and MaR9 is conferred by multiple stacked R genes. Theor Appl Genet 124(5):923-35 CrossRef
  21. Kononov ME, Bassuner B, Gelvin SB (1997) Integration of T-DNA binary vector ‘backbone-sequences into the tobacco genome: evidence for multiple complex patterns of integration. Plant J 11(5):945-57 CrossRef
  22. Kunz C, Schob H, Stam M, Kooter JM, Meins F (1996) Developmentally regulated silencing and reactivation of tobacco chitinase transgene expression. Plant J 10(3):437-50 CrossRef
  23. Lange M, Vincze E, Moller MG, Holm PB (2006) Molecular analysis of transgene and vector backbone integration into the barley genome following / Agrobacterium-mediated transformation. Plant Cell Rep 25(8):815-20 CrossRef
  24. Liu HB, Naeem MS, Liu D, Zhu YN, Guo X, Cui P, Zhou WJ (2011) Analyses of inheritance patterns and consistent expression of sporamin and chitinase PjChi-1 genes in / Brassica napus. Plant Breed 130(3):345-51 CrossRef
  25. Lowe I, Cantu D, Dubcovsky J (2011) Durable resistance to the wheat rusts: integrating systems biology and traditional phenotype-based research methods to guide the deployment of resistance genes. Euphytica 179(1):69-9 CrossRef
  26. Ooms G, Karp A, Roberts J (1983) From tumor to tuber—tumor-cell characteristics and chromosome-numbers of crown gall-derived tetraploid potato Plants ( / Solanum tuberosum cv Bard, Maris). Theor Appl Genet 66(2):169-72 CrossRef
  27. Petti C, Wendt T, Meade C, Mullins E (2009) Evidence of genotype dependency within / Agrobacterium tumefaciens in relation to the integration of vector backbone sequence in transgenic / Phytophthora infestans-tolerant potato. J Biosci Bioeng 107(3):301-06 CrossRef
  28. Que Q, Chilton MD, de Fontes CM, He C, Nuccio M, Zhu T, Wu Y, Chen JS, Shi L (2010) Trait stacking in transgenic crops: challenges and opportunities. GM Crops 1(4):220-29 CrossRef
  29. Ramanathan V, Veluthambi K (1996) Analysis of octopine left border-directed DNA transfer from / Agrobacterium to plants. J Biosci 21(1):45-6 CrossRef
  30. Revathi P, Tomar SMS, Vinod, Singh NK (2010) Marker assisted gene pyramiding of leaf rust resistance genes Lr24, Lr28 along with stripe rust resistance gene Yr15 in wheat ( / Triticum aestivum L.). Ind J Genet Plant Breed 70(4):349-54
  31. Rietman H, Bijsterbosch G, Cano L, Lee HR, Vossen J, Jacobsen E, Visser R, Kamoun S, Vleeshouwers V (2012) Qualitative and quantitative late blight resistance in the potato cultivar Sarpo Mira is determined by the perception of five distinct RXLR effectors. Mol Plant Microbe Interact 25(7):910-19 CrossRef
  32. Rommens CM, Humara JM, Ye JS, Yan H, Richael C, Zhang L, Perry R, Swords K (2004) Crop improvement through modification of the plant’s own genome. Plant Physiol 135(1):421-31 CrossRef
  33. Song L, Zhao D, Jin D (2012) Impact of T 8 transgenic rice containing an isopentenyl transferase gene on soil bacterial biomass. Adv Mater Res 455-56:1404-409 CrossRef
  34. Toevs EA, Guenthner JF, Johnson AJ, McIntosh CS, Thornton MK (2011) An industry perspective of all-native and transgenic potatoes. AgBioForum 14(1):14-9
  35. Trifonova A, Madsen S, Olesen A (2001) Agrobacterium-mediated transgene delivery and integration into barley under a range of in vitro culture conditions. Plant Sci 161(5):871-80 CrossRef
  36. U.S. Department of Agriculture (2012) Genetically engineered crops by State and United States. Available at: http://www.ers.usda.gov/datafiles/Adoption_of_Genetically_Engineered_Crops_in_the_US/alltables.xls. Accessed 17 July 2012
  37. van der Graaff E, Hooykaas PJJ (1996) Improvements in the transformation of / Arabidopsis thaliana C24 leaf-discs by / Agrobacterium tumefaciens. Plant Cell Rep 15(8):572-77 CrossRef
  38. van Engelen FA, Molthoff JW, Conner AJ, Nap JP, Pereira A, Stiekema WJ (1995) pBINPLUS: an improved plant transformation vector based on pBIN19. Transgenic Res 4(4):288-90 CrossRef
  39. Visser RGF, Hesseling-Meinders A, Jacobsen E, Nijdam H, Witholt B, Feenstra WJ (1989) Expression and inheritance of inserted markers in binary vector carrying / Agrobacterium rhizogenes–transformed potato ( / Solanum tuberosum L). Theor Appl Genet 78(5):705-14 CrossRef
  40. Vleeshouwers VGAA, van Dooijeweert W, Keizer LCP, Sijpkes L, Govers F, Colon LT (1999) A laboratory assay for / Phytophthora infestans resistance in various / Solanum species reflects the field situation. Eur J Plant Pathol 105(3):241-50 CrossRef
  41. Wang SC, Wang GY, Ding QX, Zhang H, Xie YJ, Dai JR (1999) Studies of transgene segregation and integration in maize. Acta Genet Sinica 26(3):260-61
  42. Wang C-K, Hsu S-Y, Chen P-Y, To K-Y (2009) Transformation and characterization of transgenic / Bidens pilosa L. Plant Cell Tiss Organ Cult 109(3):457-64 CrossRef
  43. Wolters AMA, Trindade LM, Jacobsen E, Visser RGF (1998) Fluorescence in situ hybridization on extended DNA fibres as a tool to analyse complex T-DNA loci in potato. Plant J 13(6):837-47 CrossRef
  44. Xu X, Pan SK, Cheng SF et al (2011) Genome sequence and analysis of the tuber crop potato. Nature 475(7355):189-95 CrossRef
  45. Yin Z, Plader W, Malepszy S (2004) Transgene inheritance in plants. J Appl Genet 45(2):127-44
  46. Zhu S, Li Y, Vossen JH, Visser RG, Jacobsen E (2012) Functional stacking of three resistance genes against / Phytophthora infestans in potato. Transgenic Res 21(1):89-9 CrossRef
  
• 作者单位：Suxian Zhu (1)
  Anita Duwal (1)
  Qi Su (1)
  Jack H. Vossen (1)
  Richard G. F. Visser (1)
  Evert Jacobsen (1)
  
  1. Wageningen UR Plant Breeding, Wageningen University and Research Center, 6708 PB, Wageningen, The Netherlands
  
• ISSN：1573-9368

文摘

Genetic transformation with resistance (R) genes is expected to enhance resistance durability against pathogens, especially for potato, a vegetatively propagated crop with tetrasomic inheritance and a long-term breeding program. In this study, 128 potato transformants were analysed for the presence of vector T-DNA genes, borders and backbone sequences. They were harvested after transformation using a construct containing neomycin phosphotransferase II (nptII) and three R genes against potato late blight (Phytophthora infestans). Our analysis revealed that 45?% of the R gene-containing transformants possessed a low T-DNA copy number, without the integration of vector backbone and borders. The integration of vector backbone sequences was characterized using eight genes, and backbone gene tetA was selected for the early prediction of plants with backbone sequence integration. Three transformants, two plants harbouring one T-DNA copy and one plant harbouring three T-DNA copies, were crossed with susceptible cv. Katahdin. Based on our results, we conclude that all four T-DNA genes were inherited as one cluster and segregated in a Mendelian fashion. The three T-DNA inserts from the transformant harbouring three T-DNA copies were statistically proven to be un-linked and inherited into the offspring plants independently. All of the R genes were functionally expressed in the offspring plants as in their parental transformants. This functional gene stacking has important implications towards achieving more durable resistance against potato late blight.