UPLC-ELSD Determination of 1-Octacosanol in Raw Material and Health Products

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• 作者：Xiaofeng Xue (1) (2) (3)
  Lanzhen Chen (1) (3)
  Jinhui Zhou (1) (3)
  Liming Wu (1) (3)
  Yi Li (1) (3)
  Fang Chen (1) (3)
  Jinzhen Zhang (1) (3)
  Jing Zhao (1) (3)
  
• 关键词：Column liquid chromatography ; Ultra performance liquid chromatography ; Evaporative light scattering detection ; 1 ; Octacosanol
• 刊名：Chromatographia
• 出版年：2012
• 出版时间：4 - February 2012
• 年：2012
• 卷：75
• 期：3
• 页码：165-168
• 全文大小：170KB
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• 作者单位：Xiaofeng Xue (1) (2) (3)
  Lanzhen Chen (1) (3)
  Jinhui Zhou (1) (3)
  Liming Wu (1) (3)
  Yi Li (1) (3)
  Fang Chen (1) (3)
  Jinzhen Zhang (1) (3)
  Jing Zhao (1) (3)
  
  1. Bee Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100093, China
  2. Department of Applied Chemistry, College of Science, China Agricultural University, Beijing, 100081, China
  3. Apicultural Branch Center, Research and Development Center of National Agro-food Processing Technology, Beijing, 102202, China
  
• ISSN：1612-1112

文摘

Determination of the levels of 1-octacosanol is important in food stuff for the study of its pharmacological activities and health benefits. In this study, a novel, simple and fast internal standard method for the non-derivatization ultra-performance liquid chromatographic determination of 1-octacosanol in raw materials and health products was developed and validated based on evaporative light scattering detection. The linearity (r 2?>?0.998), recovery (99.1-00.2%, RSD <2.7%), intra- and inter-day precision (RSD <3.8%), limit of detection (1.0?mg/L), limit of quantification (2.2?mg/L) of the 1-octacosanol were determined. The method was successfully applied to nine real 1-octacosanol products. The results of analyses had close agreement with the labeled claims of 1-octacosanol content in these products. Compared with the classical gas chromatography method, the developed method was simpler, faster and more environmentally friendly due to avoiding any derivatization step. This protocol represents a rapid and feasible method for quality control of 1-octacosanol products.