UFEIII, a fibrinolytic protease from the marine invertebrate, Urechis unicinctus

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• 作者：Qingqing Bi (1)
  Baoqin Han (1)
  Wanshun Liu (1)
  Yilin Feng (1)
  Zhongqing Jiang (1)
  
• 关键词：Fibrinolytic activity ; Serine protease ; Thrombolytic therapy ; Urechis unicinctus
• 刊名：Biotechnology Letters
• 出版年：2013
• 出版时间：July 2013
• 年：2013
• 卷：35
• 期：7
• 页码：1115-1120
• 全文大小：386KB
• 参考文献：1. Agrebi R, Haddar A, Hmidet N, Jellouli K, Manni L, Nasri M (2009) BSF1 fibrinolytic enzyme from a marine bacterium / Bacillus subtilis A26: purification, biochemical and molecular characterization. Process Biochem 44:1252-259 CrossRef
  2. Deng ZH, Wang SH, Li Q, Ji X, Zhang L, Hong M (2010) Purification and characterization of a novel fibrinolytic enzyme from the polychaete, / Neanthes japonica (Iznka). Bioresour Technol 101:1954-960 CrossRef
  3. Ju XY, Cao XY, Sun Y, Wang Z, Cao CL, Liu JJ, Jiang JH (2012) Purification and characterization of a fibrinolytic enzyme from / Streptomyces sp. XZNUM 00004. World J Microbiol Biotechnol 28:2479-486 CrossRef
  4. Mander P, Cho SS, Simkhada JR, Choi YH, Yoo JC (2011) A low molecular weight chymotrypsin-like novel fibrinolytic enzyme from / Streptomyces sp. CS624. Proc Biochem 46:1449-455 CrossRef
  5. Matsubara K, Hori K, Matsuura Y, Miyazawa K (2000) Purification and characterization of a fibrinolytic enzyme and identification of fibrinogen clotting enzyme in a marine green alga / Codium divaricatum. Comp Biochem Physiol B 125:137-43 CrossRef
  6. Nakajima N, Mihara H, Sumi H (1993) Characterization of potent fibrinolytic enzymes in earthworm, / Lumbricus rubellus. Biosci Biotechnol Biochem 57:1726-730 CrossRef
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  8. Wang DL, Han BQ, Liu WS, Xu RA (2007) Biochemical and enzymatic properties of a novel marine fibrinolytic enzyme from / Urechis unicinctus. Appl Biochem Biotechnol 136:164-51 CrossRef
  9. Wang SH, Deng ZH, Li Q, Ge X, Bo QQ, Hong M (2011) A novel alkaline serine protease with fibrinolytic activity from the polychaete, / Neanthes japonica. Comp Biochem Physiol B 159:18-5 CrossRef
  10. Zhang YL, Cui JY, Zhang R, Wang YP, Hong M (2007) A novel fibrinolytic serine protease from the polychaete / Nereis (Neanthes) virens (Sars): purification and characterization. Biochimie 89:93-03 CrossRef
  
• 作者单位：Qingqing Bi (1)
  Baoqin Han (1)
  Wanshun Liu (1)
  Yilin Feng (1)
  Zhongqing Jiang (1)
  
  1. College of Marine Life Science, Ocean University of China, Qingdao, China
  
• ISSN：1573-6776

文摘

A new serine protease with fibrinolytic activity from a marine invertebrate, Urechis unicinctus, was purified to electrophoretic homogeneity using column chromatography. SDS-PAGE of the purified enzyme showed a single polypeptide chain with MW ~20.8?kDa. Its N-terminal sequence was IIGGSQAAITSY. The purified enzyme, UFEIII, was stable at pH 6-0 below 60?°C with an optimum pH of 8.5 at approx. 55?°C. The enzyme activity was significantly inhibited by PMSF and SBTI suggesting that it was a serine protease. In fibrin plate assays, UFEIII was contained 1.46?×?103?U (urokinase units)?mg? total fibrinolytic activity, which consisted of 692?U?mg? direct fibrinolytic activity and 769?U?mg? plasminogen-activator activity. Km and Vmax values for azocasein were 1?mg?ml? and 43?μg?min??ml?, respectively.