Expression of PADI4 in hepatocellular carcinoma

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• 作者：Yang Lv (1)
  Yan Xia (1)
  Yaohua Wang (1)
  Chongyuan Cai (1)
  
• 关键词：hepatocellular carcinoma ; peptidylarginine deiminase type 4 (PAD4/PADI4) ; tumorigenic mechanism
• 刊名：The Chinese-German Journal of Clinical Oncology
• 出版年：2009
• 出版时间：August 2009
• 年：2009
• 卷：8
• 期：8
• 页码：453-455
• 全文大小：99KB
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• 作者单位：Yang Lv (1)
  Yan Xia (1)
  Yaohua Wang (1)
  Chongyuan Cai (1)
  
  1. Division I of General Surgery, Jingmen Second People鈥檚 Hospital, Jinmen, 448000, China
  

文摘

Objective The aim of the research was to study peptidylarginine deiminase type 4 (PAD4/PADI4) expression and its tumorigenic mechanism in hepatocellular carcinomas. Methods Expressions of PADI4 and p53 were investigated in tumors and non-tumor tissues by Western blot in patients with hepatocellular carcinomas. We constructed plasmid of PADI4-Flag and transfected it in Hela cells to investigate the mechanism. Results Western blot analysis showed higher PADI4 expression in hepatocellular carcinomas than in the surrounding healthy tissues. Furthermore, by Western blot, we detected decreased p53 levels in the tumor tissues of patients with hepatocellular carcinomas compared to surrounding healthy tissues. In Hela cells transfected with PcDNA3.0-Flag-PADI4 plasmid, the expression of p53 decreased obviously. Conclusion Our results suggest that PADI4 elevated in the tissues of hepatocellular carcinomas and induced tumorigenic by down-regulating p53 expression.