We report the results of a synoptic (“snapshot”) sampling of inorganic and isotopic geochemistry of surface water and groundwater during base flow in Red Canyon Creek watershed (Wyoming, USA) to evaluate how mixing of waters and geochemical processes modify stream-water chemistry. Our synoptic approach of studying the geochemistry of a stream mimics and has the same strengths of that widely used to characterize geochemical processes in groundwater systems. Gypsum dissolution, carbonate precipitation, and the influx of tributary and groundwater all affect Red Canyon Creek stream-water chemistry. Identical ranges of hydrogen and oxygen isotopes show good hydraulic connection between surface water and shallow groundwater. In contrast, δ34S
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