代谢组学在中药炮制机制研究中的应用概述
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摘要
炮制入药,是中医临床用药的三大特色之一,也是保证饮片质量和临床疗效的重要环节。中药炮制机制研究作为中药炮制学和中药现代化的关键,但是目前研究大多以化学和药理学的方法进行探讨,其深度和广度还有待加强。代谢组学作为后基因组时代新近发展起来的一门科学,现已成为中药物质基础研究的重要组成部分,并在中药炮制研究中已使用。从中药炮制减毒、增效、缓性以及生熟异用等方面论述代谢组学在中药炮制机制研究中的应用,初步分析并探讨了代谢组学应用于中药炮制机制研究所存在的问题及发展趋势。
The Chinese herbal medicine processing is one of the three major characteristics of TCM clinical medicine.It is also an important link in guarantee the quality of slices and clinical curative effect. At present,the width and the depth of the study on the mechanism of traditional processing is not enough. The chemical and pharmacology methods are mainly used to discuss the mechanism of traditional processing. Study on the processing mechanism of traditional Chinese medicine is the key to science of processing Chinese materia medica and modernization of traditional Chinese medicine.Metabonomics is a newly developed a science at postgenome era and has become an important part of Chinese medicine material basic research. At present,metabonomics has started to use in the study of traditional processing. This paper introduced the application of metabonomics in the research of the processing principle of attenuated,synergia,delayed or cooked with different treatments. The preliminary analysis discusses the research problems and development trend on the metabonomics applying in the processing mechanism of TCM.
The Chinese herbal medicine processing is one of the three major characteristics of TCM clinical medicine.It is also an important link in guarantee the quality of slices and clinical curative effect. At present,the width and the depth of the study on the mechanism of traditional processing is not enough. The chemical and pharmacology methods are mainly used to discuss the mechanism of traditional processing. Study on the processing mechanism of traditional Chinese medicine is the key to science of processing Chinese materia medica and modernization of traditional Chinese medicine.Metabonomics is a newly developed a science at postgenome era and has become an important part of Chinese medicine material basic research. At present,metabonomics has started to use in the study of traditional processing. This paper introduced the application of metabonomics in the research of the processing principle of attenuated,synergia,delayed or cooked with different treatments. The preliminary analysis discusses the research problems and development trend on the metabonomics applying in the processing mechanism of TCM.
引文
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[31]李震宇,范玛莉,秦雪梅.基于NMR代谢组学技术的白芍及其醋制品的化学比较[J].药学学报,2015,50(2):211-217.
[32]顾薇,陆兔林,李金慈,等.莪术醋制对大鼠胆汁代谢的影响[J].中国中药杂志,2016,41(7):1318-1324.
[33]张敏,焦宏,郭捷.基于NMR代谢组学技术对山药化学成分的研究[J].食品工程,2014(4):19-23.
[34]莫毛燕,朱琼花,薛兴阳,等.姜炭炮制前后对虚寒性出血症大鼠尿液代谢组学分析[J].中国实验方剂学杂志,2015(16):1-4.
[35]苏锦波,朱琼花,薛兴阳,等.姜炭温经止血作用的血清代谢组学研究[J].中药材,2015,38(6):1177-1179.
[36]陈志敏,崔园园,张美,等.基于尿液代谢组学的二神丸中补骨脂、肉豆蔻炮制增效作用的机理研究[J].中药新药与临床药理,2015(6):731-734.
[37]陈志敏,胡昌江,熊瑞,等.“二神丸”中补骨脂、肉豆蔻炮制前后对脾肾阳虚泄泻大鼠血清代谢组学的影响[J].中国中药杂志,2015,40(7):1400-1403.
[38]钟凌云,廖智慧,龚千锋,等.基于代谢组学研究姜制对黄连药性的影响[J].中草药,2013,44(22):3177-3181.
[39]王雪洁,李震宇,薛水玉,等.基于植物代谢组学技术的远志不同炮制品质量控制研究[J].中草药,2012,43(9):1727-1737.
[40]韩伟健,林晓彤,郭娜,等.泽泻盐制前后成分转化的1H NMR分析[J].波谱学杂志,2016,33(1):117-124.
[41]陈燕燕,李晓男,王跃飞,等.应用核磁共振指纹谱考察黄芪不同炮制品化学成分的差异[J].中国科技论文,2014,9(12):1410-1413.
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[2]Pang X,Hua X,Yang Q,et al.Inter-species transplantation of gut microbiota from human to pigs[J].The Isme Journal,2007,1(2):156-162.
[3]盛佳钰,苏永华.基于代谢组学的中药新药研究概况[J].江苏中医药,2012,44(5):73-75.
[4]赵珊,王鹏程,冯健,等.代谢组学技术及其在中医药研究中的应用[J].中草药,2015,46(5):756-765.
[5]Ott K H,Aranibar N.Nuclear magnetic resonance metabonomics:methods for drug discovery and development[J].Methods in Molecular Biology,2007,358(2):247-271.
[6]Fang J,Wang W,Sun S,et al.Metabolomics study of renal fibrosis and intervention effects of total aglycone extracts of Scutellaria baicalensis in unilateral ureteral obstruction rats[J].Journal of Ethnopharmacology,2016,192(4):20-29.
[7]Wang Y,Guo X,Xie J,et al.A GC-MS Based Metabonomics Study of Rheumatoid Arthritis and the Interventional Effects of the Simiaowan in Rats[J].Molecules,2015,20(12):21364-21372.
[8]Chen M,Ni Y,Duan H,et al.Mass spectrometry-based metabolic profiling of rat urine associated with general toxicity induced by the multiglycoside of Tripterygium wilfordii Hook.f[J].Chemical Research in Toxicology,2008,21(2):288-294.
[9]Yan S,Wu B,Lin Z,et al.Metabonomic characterization of aging and investigation on the anti-aging effects of total flavones of Epimedium[J].Molecular Biosystems,2009,5(10):1204-1213.
[10]Tan Y,Liu X,Lu C,et al.Metabolic profiling reveals therapeutic biomarkers of processed Aconitum Carmichaeli Debx,in treating hydrocortisone induced Kidney-Yang deficiency syndrome rats[J].Journal of Ethnopharmacology,2014,152(3):585-593.
[11]Zhang Y,Zhang A,Zhang Y,et al.Application of Ultra-performance Liquid Chromatography with Time-of-Flight Mass Spectrometry for the Rapid Analysis of Constituents and Metabolites from the Extracts of Acanthopanax senticosus Harms Leaf[J].Pharmacognosy Magazine,2015,12(46):145-152.
[12]高家荣,方朝晖,庄星星,等.基于UPLC/QTOF-MS技术的丹蛭降糖胶囊防治2型糖尿病血管病变大鼠的血清代谢组学研究[J].中国医院药学杂志,2016(11):878-883.
[13]Kuang H,Wang Y,Wang Q,et al.Metabonomics Research of the Four Properties in Traditional Chinese Medicine Based on UPLC-QTOF-MS System[M].Recent Advances in Theories and Practice of Chinese Medicine,2012.
[14]Timothy M.D.Ebbels,Beckonert K O P.Prediction and Classification of Drug Toxicity Using Probabilistic Modeling of Temporal Metabolic Data:The Consortium on Metabonomic Toxicology Screening Approach[J].Journal of Proteome Research,2007,6(11):4407-4422.
[15]刘昌孝,陈士林,肖小河,等.中药质量标志物(Q-Marker):中药产品质量控制的新概念[J].中草药,2016,47(9):1443-1457.
[16]赵楠,张晓哲,胡昌江,等.运用代谢组学技术研究发现炮制引起大黄多成分变化[J].中国中药杂志,2014,39(9):1607-1613.
[17]徐珊,张凡,刘蓬蓬,等.基于大鼠物质、能量代谢研究炮制对黄柏药性的影响[J].中药材,2015(9):1835-1841.
[18]吴莹,高慧,宋泽璧.盐知母不同有效部位改善链脲佐菌素诱导2型糖尿病大鼠糖代谢的研究[J].现代药物与临床,2016,31(3):280-283.
[19]丁霞,朱方石,余宗亮,等.山茱萸炮制前后宏微量元素及氨基酸成分比较研究[J].中药材,2007,30(4):396-399.
[20]王敏江,林佩,李云飞,等.何首乌不同炮制品对肝细胞脂代谢的干预作用研究[J].现代药物与临床,2013,28(4):494-499.
[21]曾颜,侯朋艺,陈晓辉.基于植物代谢组学技术的京大戟炮制前后化学成分变化研究[J].中药材,2016,39(3):530-533.
[22]窦娟.中药藤黄炮制解毒的研究[D].南京:南京中医药大学,2013.
[23]李艺,范玛莉,邢婕,等.龟龄集中细辛特殊炮制工艺的代谢组学研究[J].中草药,2015,46(16):2385-2393.
[24]李伶.附子及其主要毒性成分对大鼠毒性的代谢组学研究[D].北京:中国人民解放军军事医学科学院,2007.
[25]唐冰雯,丁佳佳,杨永霞,等.基于NMR的代谢组学对醋制甘遂减毒作用的研究[J].中药药理与临床,2013,29(4):98-103.
[26]李娟,张松,秦雪梅,等.基于NMR代谢组学技术的款冬花生品与蜜炙品化学成分比较[J].中草药,2015,46(20):3009-3016.
[27]戴建业,郑宁宁,孙淑军,等.基于血清代谢组学的柴胡醋炙生物学效应研究[J].中华中医药学刊,2014(7):1717-1720.
[28]钟立甲,张文泉,华永丽,等.代谢组学方法评价当归不同炮制品挥发油抗大鼠急性炎症作用的研究[J].中国中药杂志,2016,41(11):2061-2069.
[29]薛子钰,华永丽,李锦霞,等.当归不同炮制品挥发油给药大鼠的尿液代谢组学研究[J].中国中药杂志,2016,41(5):928-939.
[30]Zhong L J,Hua Y L,Ji P,et al.Evaluation of the anti-inflammatory effects of volatile oils from processed products of Angelica sinensis,radix by GC-MS-based metabolomics[J].Journal of Ethnopharmacology,2016,191:195-205.
[31]李震宇,范玛莉,秦雪梅.基于NMR代谢组学技术的白芍及其醋制品的化学比较[J].药学学报,2015,50(2):211-217.
[32]顾薇,陆兔林,李金慈,等.莪术醋制对大鼠胆汁代谢的影响[J].中国中药杂志,2016,41(7):1318-1324.
[33]张敏,焦宏,郭捷.基于NMR代谢组学技术对山药化学成分的研究[J].食品工程,2014(4):19-23.
[34]莫毛燕,朱琼花,薛兴阳,等.姜炭炮制前后对虚寒性出血症大鼠尿液代谢组学分析[J].中国实验方剂学杂志,2015(16):1-4.
[35]苏锦波,朱琼花,薛兴阳,等.姜炭温经止血作用的血清代谢组学研究[J].中药材,2015,38(6):1177-1179.
[36]陈志敏,崔园园,张美,等.基于尿液代谢组学的二神丸中补骨脂、肉豆蔻炮制增效作用的机理研究[J].中药新药与临床药理,2015(6):731-734.
[37]陈志敏,胡昌江,熊瑞,等.“二神丸”中补骨脂、肉豆蔻炮制前后对脾肾阳虚泄泻大鼠血清代谢组学的影响[J].中国中药杂志,2015,40(7):1400-1403.
[38]钟凌云,廖智慧,龚千锋,等.基于代谢组学研究姜制对黄连药性的影响[J].中草药,2013,44(22):3177-3181.
[39]王雪洁,李震宇,薛水玉,等.基于植物代谢组学技术的远志不同炮制品质量控制研究[J].中草药,2012,43(9):1727-1737.
[40]韩伟健,林晓彤,郭娜,等.泽泻盐制前后成分转化的1H NMR分析[J].波谱学杂志,2016,33(1):117-124.
[41]陈燕燕,李晓男,王跃飞,等.应用核磁共振指纹谱考察黄芪不同炮制品化学成分的差异[J].中国科技论文,2014,9(12):1410-1413.
[42]吴皓,胡昌江.中药炮制学[M].北京:人民卫生出版社,2012.
[43]高飞,傅超美,胡慧玲,等.HPLC-UV技术用于川木香煨制前后代谢物轮廓差异研究[J].中草药,2013,44(5):547-551.
[44]周路明.何首乌生熟异用代谢组学、复方配伍系统性研究[D].昆明:云南中医学院,2014.
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