麝香草酚抗氧化和抑制酪氨酸酶活性研究
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摘要
目的检测麝香草酚的抗氧化活性和抑制酪氨酸酶活性。方法采用清除DPPH、ABTS、羟自由基、超氧自由基,抑制脂质过氧化活性和抑制酪氨酸酶活性的方法,检测麝香草酚的抗氧化活性和抑制酪氨酸酶活性。结果麝香草酚具有明显的抗氧化活性和抑制酪氨酸酶活性,呈浓度依赖性效应。在清除DPPH、ABTS、羟自由基、超氧自由基,抑制脂质过氧化活性和抑制酪氨酸酶活性试验中,麝香草酚的体系终浓度IC_(50)分别为120,40.9,24,60,29.17μmol/L和37.49μmol/L。作为阳性对照药物的山柰酚的体系终浓度IC_(50)分别为80,9.09,12,12,8.33μmol/L和3.33μmol/L。麝香草酚具有1个酚羟基,山柰酚具有3个酚羟基,分子结构的不同,导致麝香草酚、山柰酚不同的抗氧化活性和抑制酪氨酸酶活性。结论研究表明麝香草酚具有明显的抗氧化活性和抑制酪氨酸酶活性,是值得进一步研究的抗氧化防衰老类候选药物、化妆品原料和食品添加剂。
The experiment examined the antioxidant and antityrosinase effects of thymol on DPPH,ABTS,hydroxyl,superoxide radical scavenging activity,and lipid peroxidation inhibition activity,and antityrosinase activity.The experiment results showed that thymol has excellent antioxidant and antityrosinase activities in dose-dependent manner.In the experiment of scavening DPPH,ABTS,hydroxyl,superoxide radicals,inhibiting lipid peroxidation,and antityrosinase activity,the IC_(50) of thymol was respectively 120μmol/L,40.9μmol/L,24μmol/L,60μmol/L,29.17μmol/L,and 37.49μmol/L.The IC_(50) of kaempferol used as the positive control drug was respectively 80μmol/L,9.09μmol/L,12μmol/L,12μmol/L,8.33μmol/L,and 3.33μmol/L.Thymol has one phenolic hydroxyl,while kaempferol has three phenolic hydroxyls.The different molecular structures lead to the different antioxidant and antityrosinase activities.In a word,thymol has good antioxidant and antityrosinase activities,which supports the use of thymol as the new anti-aging candidate drug,cosmetic materials and food additives.
The experiment examined the antioxidant and antityrosinase effects of thymol on DPPH,ABTS,hydroxyl,superoxide radical scavenging activity,and lipid peroxidation inhibition activity,and antityrosinase activity.The experiment results showed that thymol has excellent antioxidant and antityrosinase activities in dose-dependent manner.In the experiment of scavening DPPH,ABTS,hydroxyl,superoxide radicals,inhibiting lipid peroxidation,and antityrosinase activity,the IC_(50) of thymol was respectively 120μmol/L,40.9μmol/L,24μmol/L,60μmol/L,29.17μmol/L,and 37.49μmol/L.The IC_(50) of kaempferol used as the positive control drug was respectively 80μmol/L,9.09μmol/L,12μmol/L,12μmol/L,8.33μmol/L,and 3.33μmol/L.Thymol has one phenolic hydroxyl,while kaempferol has three phenolic hydroxyls.The different molecular structures lead to the different antioxidant and antityrosinase activities.In a word,thymol has good antioxidant and antityrosinase activities,which supports the use of thymol as the new anti-aging candidate drug,cosmetic materials and food additives.
引文
[1]胡艳芬,江文斌,蒋琳琳,等.高百里香酚和麝香草酚体外抑菌作用的研究[J].畜牧兽医科技信息,2010,26(10):107.
[2]王娣,许晖,钱时权,等.麝香草酚对食品常见污染菌的抑菌作用[J].食品科学,2013,34(3):119.
[3]王娣,谢海伟,曹珂珂,等.麝香草酚抑菌活性及其影响因素研究[J].食品工业科技,2012,34(14):96.
[4]闭兴明,孟日增,郭娜,等.麝香草酚体外抗真菌活性研究[J].中国农学通报,2009,26(1):21.
[5]刘敏,肖颖,左爱仁.根皮素、槲皮素、水飞蓟宾清除自由基和抑制脂质过氧化活性研究[J].中成药,2012,28(11):75.
[6]Lee YL,Yang JH,Mau JL.Antioxidant properties of water extracts from Monascus fermented soybeans[J].Food Chem,2008,106:1127.
[7]Re R,Pellegrini N,Proteggente A,et al.Antioxidant activity applying an improved ABTS radical cation decolorization assay[J].Free Radic Biol Med,1999,26(9-10):1231.
[8]De Avellar IG,Magalhaas MM,Silvan AB,et al.Reevaluating the role of 1,10-phenanthroline in oxidative reactions involving ferrous ions and DNA damage[J].Biochim Biophys Acta,2004,1675(3):46.
[9]Marklund S,Marklund G.Involvement of the superoxide anion radical in theautoxidation of pyrogallal and a convenient assay for superoxide dismutase[J].Eur J Biochem,1974,47(3):469.
[10]刘敏,邵文祥,张灵芝,等.异鼠李素对蘑菇酪氨酸酶二酚酶活力的抑制机理[J],中国食品添加剂,2016,27(12):23.
[11]王锐,何嵋,周云,等.山柰提取物抗氧化性能研究[J].广东农业科学2011,156(6):156.
[12]童方念,罗超,罗丹,等.山柰酚对四氯化碳致小鼠急性肝损伤的保护作用[J].西安交通大学学报(医学版),2014,35(6):816.
[2]王娣,许晖,钱时权,等.麝香草酚对食品常见污染菌的抑菌作用[J].食品科学,2013,34(3):119.
[3]王娣,谢海伟,曹珂珂,等.麝香草酚抑菌活性及其影响因素研究[J].食品工业科技,2012,34(14):96.
[4]闭兴明,孟日增,郭娜,等.麝香草酚体外抗真菌活性研究[J].中国农学通报,2009,26(1):21.
[5]刘敏,肖颖,左爱仁.根皮素、槲皮素、水飞蓟宾清除自由基和抑制脂质过氧化活性研究[J].中成药,2012,28(11):75.
[6]Lee YL,Yang JH,Mau JL.Antioxidant properties of water extracts from Monascus fermented soybeans[J].Food Chem,2008,106:1127.
[7]Re R,Pellegrini N,Proteggente A,et al.Antioxidant activity applying an improved ABTS radical cation decolorization assay[J].Free Radic Biol Med,1999,26(9-10):1231.
[8]De Avellar IG,Magalhaas MM,Silvan AB,et al.Reevaluating the role of 1,10-phenanthroline in oxidative reactions involving ferrous ions and DNA damage[J].Biochim Biophys Acta,2004,1675(3):46.
[9]Marklund S,Marklund G.Involvement of the superoxide anion radical in theautoxidation of pyrogallal and a convenient assay for superoxide dismutase[J].Eur J Biochem,1974,47(3):469.
[10]刘敏,邵文祥,张灵芝,等.异鼠李素对蘑菇酪氨酸酶二酚酶活力的抑制机理[J],中国食品添加剂,2016,27(12):23.
[11]王锐,何嵋,周云,等.山柰提取物抗氧化性能研究[J].广东农业科学2011,156(6):156.
[12]童方念,罗超,罗丹,等.山柰酚对四氯化碳致小鼠急性肝损伤的保护作用[J].西安交通大学学报(医学版),2014,35(6):816.
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