不同果形栀子鲜品不同极性部位HPLC指纹图谱
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摘要
目的:研究并比较不同果形栀子鲜品不同极性部位(石油醚部位、三氯甲烷部位、乙酸乙酯部位、正丁醇部位、水部位)指纹图谱,深入了解其化学成分含量差异及分布情况。方法:将栀子用水回流提取,得水提取物,取水提物0. 1 g,用水50 m L溶解后,依次用石油醚、三氯甲烷、乙酸乙酯、正丁醇进行萃取,得到各萃取相及萃取后的水相,浓缩至浸膏。建立HPLC指纹图谱,并采用相似度评价系统软件进行数据分析。结果:不同产地栀子水提物三氯甲烷部位指纹图谱可以将福建、河南、江西产栀子分开。福建栀子水提物与河南、江西的区别主要体现在石油醚部位,且其脂溶性成分比河南、江西栀子多;河南栀子水提物与福建、江西栀子的区别主要体现在乙酸乙酯部位,且其环烯迷萜苷类成分含量明显高于福建、江西栀子。江西栀子水提物与河南、福建栀子的差异主要体现在正丁醇部位,其无有机酸类峰C1。六、七棱栀子:栀子水提物的三氯甲烷部位指纹图谱可以将福建与河南的六、七棱栀子区分开,且七棱栀子各成分含量均大于六棱;栀子水提物的石油醚和乙酸乙酯部位指纹图谱均可以将江西六、七棱栀子区分开。河南六棱栀子Z3峰明显高于七棱;江西七棱栀子水提物三氯甲烷、乙酸乙酯部位各成分含量大于六棱。结论:福建、河南、江西栀子化学成分含量及种类存在显著差异;六、七棱栀子图谱差异主要体现在峰高。
Objective: To investigate and compare the fingerprints of different polarity fractions( petroleumether,chloroform,ethyl acetate,n-butanol,water) of fresh Gardeniae Fructus with different fruit shapes,and further understand the content difference and distribution of its chemical composition. Method:Gardeniae Fructus was reflux extracted by water in order to obtain the water extract; water extract 0. 1 g and dissolved with 50 m L water,then it was extracted by petroleum ether,chloroform,ethyl acetate and n-butanol in turn in order to obtain the different extraction phases and the water phase. Each phase was condensed to extractum.Finally,the samples were analyzed by high performance liquid chromatography( HPLC) fingerprints and the similarity evaluation software was used for data analysis. Result: Fingerprint of chloroform fraction of water extract in gardenia from different habitats can be used to distinguish Gardeniae Fructus from Fujian,Henan and Jiangxi.The differences between the water extract of Gardeniae Fructus from Fujian and those of Henan and Jiangxi were mainly manifested in the petroleum ether fraction,and the fat-soluble components of Gardeniae Fructus were more than those of Henan and Jiangxi. The differences between the water extract of Gardeniae Fructus from Henan and those of Fujian and Jiangxi were mainly manifested in the ethyl acetate fraction, and the content of iridoid glycosides was significantly higher than that in Fujian and Henan. The differences between the water extract of gardenia from Jiangxi and those of Fujian and Henan were mainly manifested in the n-butanol fraction,organic acid peak C1 not detected. The fingerprint of chloroform fraction of water extract in Gardeniae Fructus can be used to distinguish Gardeniae Fructus of six ribs and Gardeniae Fructus of seven ribs from Fujian and Henan,and the contents of all components of Gardeniae Fructus of seven ribs were more than those in Gardeniae Fructus of six ribs.The fingerprint of petroleum ether fraction of water extract in Gardeniae Fructus can be used to distinguish Gardeniae Fructus of six ribs and Gardeniae Fructus of seven ribs from Jiangxi. The Z3 peak of Gardeniae Fructus of six ribs from Henan was obviously higher than that of Gardeniae Fructus of seven ribs. The contents of all components on chloroform and ethyl acetate fractions of water extract in Gardeniae Fructus of seven ribs were significantly higher than those of Gardeniae Fructus of six ribs. Conclusion: There are significant differences on chemical constituents and content among Gardeniae Fructus from Fujian,Henan and Jiangxi. The main difference of fingerprint between Gardeniae Fructus of six ribs and Gardeniae Fructus of seven ribs is the peak height.
Objective: To investigate and compare the fingerprints of different polarity fractions( petroleumether,chloroform,ethyl acetate,n-butanol,water) of fresh Gardeniae Fructus with different fruit shapes,and further understand the content difference and distribution of its chemical composition. Method:Gardeniae Fructus was reflux extracted by water in order to obtain the water extract; water extract 0. 1 g and dissolved with 50 m L water,then it was extracted by petroleum ether,chloroform,ethyl acetate and n-butanol in turn in order to obtain the different extraction phases and the water phase. Each phase was condensed to extractum.Finally,the samples were analyzed by high performance liquid chromatography( HPLC) fingerprints and the similarity evaluation software was used for data analysis. Result: Fingerprint of chloroform fraction of water extract in gardenia from different habitats can be used to distinguish Gardeniae Fructus from Fujian,Henan and Jiangxi.The differences between the water extract of Gardeniae Fructus from Fujian and those of Henan and Jiangxi were mainly manifested in the petroleum ether fraction,and the fat-soluble components of Gardeniae Fructus were more than those of Henan and Jiangxi. The differences between the water extract of Gardeniae Fructus from Henan and those of Fujian and Jiangxi were mainly manifested in the ethyl acetate fraction, and the content of iridoid glycosides was significantly higher than that in Fujian and Henan. The differences between the water extract of gardenia from Jiangxi and those of Fujian and Henan were mainly manifested in the n-butanol fraction,organic acid peak C1 not detected. The fingerprint of chloroform fraction of water extract in Gardeniae Fructus can be used to distinguish Gardeniae Fructus of six ribs and Gardeniae Fructus of seven ribs from Fujian and Henan,and the contents of all components of Gardeniae Fructus of seven ribs were more than those in Gardeniae Fructus of six ribs.The fingerprint of petroleum ether fraction of water extract in Gardeniae Fructus can be used to distinguish Gardeniae Fructus of six ribs and Gardeniae Fructus of seven ribs from Jiangxi. The Z3 peak of Gardeniae Fructus of six ribs from Henan was obviously higher than that of Gardeniae Fructus of seven ribs. The contents of all components on chloroform and ethyl acetate fractions of water extract in Gardeniae Fructus of seven ribs were significantly higher than those of Gardeniae Fructus of six ribs. Conclusion: There are significant differences on chemical constituents and content among Gardeniae Fructus from Fujian,Henan and Jiangxi. The main difference of fingerprint between Gardeniae Fructus of six ribs and Gardeniae Fructus of seven ribs is the peak height.
引文
[1]国家药典委员会.中华人民共和国药典.一部[M].北京:中国医药科技出版社,2015:248.
[2]曹进,徐燕,张永知,等.栀子药材的指纹图谱整体性分析[J].分析化学,2004,32(7):875-878.
[3]孟祥乐,李红伟,李颜,等.栀子化学成分及其药理作用研究进展[J].中国新药杂志,2011,20(11):959-967.
[4]刘武占,范建伟,高艳红,等. HPLC同时测定栀子中8个环烯醚萜苷类成分的含量[J].中国中药杂志,2012,37(16):2417-2421.
[5]张燕,朱华旭,郭立玮.栀子中环烯醚萜类化合物的体内过程及其对相关酶的影响[J].中国中药杂志,2012,37(3):269-273.
[6]裴彩云,蔡艳,宋剑,等. 10批栀子中环烯醚萜苷类成分含量测定[J].中国现代应用药学,2011,28(S1):1343-1345.
[7]邓颖,郭志刚,曾兆麟,等.藏红花的药理研究进展[J].中国中药杂志,2002,27(8):565.
[8]王亭.中药栀子有效成分及药理作用的研究进展[J].中国药师,2015,18(10):1782-1784.
[9]任德权.中药指纹图谱质控技术的意义与作用[J].中药材,2001,24(4):235-239.
[10]姚超,辛华,陆兔林,等.不同产地栀子的超高效液相色谱指纹图谱及模式识别研究[J].中国药学杂志,2017,52(1):63-67.
[11]鲍家科,刘玲.栀子药材及其炮制品的指纹图谱对比[J].中国医药工业杂志,2016,47(2):163-167.
[12]闫光军,范建伟,苏瑞强,等.不同采收期栀子有机酸类成分HPLC指纹图谱[J].中国实验方剂学杂志,2015,21(11):39-41.
[13]苗琦,罗光明,罗扬婧,等.栀子多波长HPLC指纹图谱及化学模式识别研究[J].中草药,2014,45(21):3159-3164.
[14]韩颖.栀子药材的指纹图谱研究[D].武汉:湖北中医药大学,2014.
[15]罗光明,陈岩,张晓云,等.不同品种及产地栀子水溶性成分指纹图谱研究[J].中成药,2008,30(4):475-479.
[16]杨相玉.栀子的指纹图谱与提取工艺研究[D].济南:山东大学,2009.
[17]徐燕,曹进,王义明,等.多波长高效液相色谱法同时测定栀子中的三类成分[J].药学学报,2003,38(7):543-546.
[18]刘小花,白仲梅,梁瑾,等.黄芪药材不同极性部位的指纹图谱[J].兰州大学学报:自然科学版,2013,49(4):573-580.
[2]曹进,徐燕,张永知,等.栀子药材的指纹图谱整体性分析[J].分析化学,2004,32(7):875-878.
[3]孟祥乐,李红伟,李颜,等.栀子化学成分及其药理作用研究进展[J].中国新药杂志,2011,20(11):959-967.
[4]刘武占,范建伟,高艳红,等. HPLC同时测定栀子中8个环烯醚萜苷类成分的含量[J].中国中药杂志,2012,37(16):2417-2421.
[5]张燕,朱华旭,郭立玮.栀子中环烯醚萜类化合物的体内过程及其对相关酶的影响[J].中国中药杂志,2012,37(3):269-273.
[6]裴彩云,蔡艳,宋剑,等. 10批栀子中环烯醚萜苷类成分含量测定[J].中国现代应用药学,2011,28(S1):1343-1345.
[7]邓颖,郭志刚,曾兆麟,等.藏红花的药理研究进展[J].中国中药杂志,2002,27(8):565.
[8]王亭.中药栀子有效成分及药理作用的研究进展[J].中国药师,2015,18(10):1782-1784.
[9]任德权.中药指纹图谱质控技术的意义与作用[J].中药材,2001,24(4):235-239.
[10]姚超,辛华,陆兔林,等.不同产地栀子的超高效液相色谱指纹图谱及模式识别研究[J].中国药学杂志,2017,52(1):63-67.
[11]鲍家科,刘玲.栀子药材及其炮制品的指纹图谱对比[J].中国医药工业杂志,2016,47(2):163-167.
[12]闫光军,范建伟,苏瑞强,等.不同采收期栀子有机酸类成分HPLC指纹图谱[J].中国实验方剂学杂志,2015,21(11):39-41.
[13]苗琦,罗光明,罗扬婧,等.栀子多波长HPLC指纹图谱及化学模式识别研究[J].中草药,2014,45(21):3159-3164.
[14]韩颖.栀子药材的指纹图谱研究[D].武汉:湖北中医药大学,2014.
[15]罗光明,陈岩,张晓云,等.不同品种及产地栀子水溶性成分指纹图谱研究[J].中成药,2008,30(4):475-479.
[16]杨相玉.栀子的指纹图谱与提取工艺研究[D].济南:山东大学,2009.
[17]徐燕,曹进,王义明,等.多波长高效液相色谱法同时测定栀子中的三类成分[J].药学学报,2003,38(7):543-546.
[18]刘小花,白仲梅,梁瑾,等.黄芪药材不同极性部位的指纹图谱[J].兰州大学学报:自然科学版,2013,49(4):573-580.
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