嗜盐高效降酚菌株Halomonas sp. H17的筛选及降解苯酚特性

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英文篇名：Screening and phenol-degrading characteristics of a highly efficient phenol-degrading halophilic bacterial strain Halomonas sp. H17 作者：赵娜娜 ; 许继飞 ; 宋晓雪 ; 田鹏 ; 丁舒心 ; 赵吉 英文作者：ZHAO Nana;XU Jifei;SONG Xiaoxue;TIAN Peng;DING Shuxin;ZHAO Ji;Inner Mongolia Key Laboratory of Environmental Pollution Control and Waste Resource Recycle;School of Ecology and Environment, Inner Mongolia University;Shanghai Normal University Tianhua College; 关键词：嗜盐菌 ; Halomonas ; sp. ; 苯酚 ; 生物降解 ; 动力学 英文关键词：Halophilic bacteria;;Halomonas sp;;phenol;;biodegradation;;kinetics 中文刊名：HJXX 英文刊名：Acta Scientiae Circumstantiae 机构：内蒙古大学环境污染控制与废物资源化自治区重点实验室;内蒙古大学生态与环境学院;上海师范大学天华学院; 出版日期：2018-10-23 14:56 出版单位：环境科学学报 年：2019 期：v.39 基金：内蒙古自治区自然科学基金(No.2016MS0305) 语种：中文; 页：HJXX201902005 页数：7 CN：02 ISSN：11-1843/X 分类号：43-49

摘要

从巴丹吉林沙漠盐湖沉积物中分离获得1株在高盐环境下高效降解苯酚菌H17.分析了H17生理生化特性、16S rDNA基因序列、苯酚降解特性及动力学,结果表明,H17属于盐单胞菌属(Halomonas sp.),能在0～20%的盐度下有效降解苯酚,每升外加适量的碳源(葡萄糖浓度0.8 g)和复合氮源(KNO_3 1 g、NH_4Cl 5 g、酵母提取物0.2 g和胰蛋白胨0.2 g)能够促进H17的生长及降解苯酚能力.在温度为30℃、pH 7～8、盐度5～10%的条件下,H17均能高效降解苯酚,最高降解率可达到88.5%.该菌株降解苯酚动力学符合Halane模型,经拟合其生长参数为μ_(max)=0.31 h~(-1),K_S=191.63 mg·L~(-1),K_i=683.05 mg·L~(-1).研究显示H17具有在高盐环境下降解和耐受苯酚的能力,同时对环境有较强的适应性,体现出其在高盐含酚废水实际处理中具有良好的应用价值.
        A highly efficient phenol-degrading bacterial strain was separated from the sediments of Badain Jaran Desert Salt Lake in the hypersaline condition. The physiological and biochemical characteristics, 16 S rDNA gene sequences, and phenol-degrading kinetics were analyzed. The results revealed that the bacterial strain H17 belonged to Halomonas sp., which could efficiently degrade phenol under 0～20% salinity. Furthermore, adding appropriate carbon source(0.8g glucose) and nitrogen sources(i.e. 1 g KNO_3, 5 g NH_4Cl, 0.2 g yeast extract, and 0.2 g tryptone) could promote the growth of H17 as well as phenol degradation. Under optimal degradation conditions(30 ℃, pH 7～8, salinity 5%～10%), the highest degradation rate of phenol by H17 could reach up to 88.5%. The phenol-degrading kinetics of this bacterial strain was characterized using Halane-model, with the growth parameters of μ_(max)=0.31 h~(-1),K_S=191.63 mg·L~(-1),K_i=683.05 mg·L~(-1) according to the over-fitting. This study showed that the bacterial strain H17 could tolerate and degrade phenol in the hyperhaline environment, suggestive of its application value for hyperhaline phenolic wastewater treatments.

引文

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