鼠疫菌pMT1质粒的序列分析研究进展
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摘要
鼠疫是由鼠疫耶尔森菌(简称鼠疫菌)引起的烈性传染病。鼠疫菌质粒是鼠疫菌染色体外的遗传物质,3种常规质粒中,p MT1质粒序列变化最大,结构组成最复杂,目前对其研究相对较少。应用BLAST和Mauve软件对已有36株菌株的pMT1质粒序列比对,对鼠疫菌pMT1质粒的序列特征、基因及其功能研究分析。结果显示基因模块存在较大重排,模块排列存在较大差异,同一生物型有可能差异较大,不同生物型也有模块相似,但同一生物型较不同生物型差异相对较小;插入序列数及位置不同,部分菌株pMT1质粒序列上的毒力基因存在基因突变;一些菌株的pMT1质粒序列中含有其他菌株没有的特殊基因,以及其他功能尚不清楚的基因。对鼠疫菌pMT1质粒的序列分析研究,可以增进对鼠疫流行的认识,对疫苗开发及制定合理的防治对策具有重要意义。
Plague is a fulminating infectious disease caused by Yersinia pestis(Y. pestis). The plasmids areextrachromosomal genetic materials of Y. pestis. Among the three common plasmids, pMT1 has the greatest sequence variationand the most complex structure composition. At present, there are relatively few research on pMT1. BLAST and Mauve softwarewere used to compare the pMT1 plasmid sequences of 36 strains, and the sequence characteristics, genes and functions of Y.pestis p MT1 plasmids were analyzed. The results showed that there were large rearrangements of gene modules and largedifferences in module arrangement. The same biotype had large differences, and different biotypes also had similar modules,but the difference of same biotype were relatively small. The number and positions of insertion sequences were different, somestrains had mutations in the virulence gene of the pMT1 plasmid sequence; some strains contained special genes that not foundin other strains, and other genes whose functions were still unclear. Sequence analysis of the pMT1 plasmid of Y. pestis canenhance the understanding of the plague epidemic, which has great significance for the development of plague vaccine andformulation reasonable plague prevention and control measures.
Plague is a fulminating infectious disease caused by Yersinia pestis(Y. pestis). The plasmids areextrachromosomal genetic materials of Y. pestis. Among the three common plasmids, pMT1 has the greatest sequence variationand the most complex structure composition. At present, there are relatively few research on pMT1. BLAST and Mauve softwarewere used to compare the pMT1 plasmid sequences of 36 strains, and the sequence characteristics, genes and functions of Y.pestis p MT1 plasmids were analyzed. The results showed that there were large rearrangements of gene modules and largedifferences in module arrangement. The same biotype had large differences, and different biotypes also had similar modules,but the difference of same biotype were relatively small. The number and positions of insertion sequences were different, somestrains had mutations in the virulence gene of the pMT1 plasmid sequence; some strains contained special genes that not foundin other strains, and other genes whose functions were still unclear. Sequence analysis of the pMT1 plasmid of Y. pestis canenhance the understanding of the plague epidemic, which has great significance for the development of plague vaccine andformulation reasonable plague prevention and control measures.
引文
[1]申小娜,王琪,夏连续,等.我国新发现的鼠疫自然疫源地鼠疫菌基因组序列测定及分析[J].中国地方病学杂志,2011,30(5):476-480.
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[14]SONG Y.Complete genome sequence of Yersinia pestis strain91001,an isolate avirulent to humans[J].DNA Research,2004,11(3):179-197.
[15]KIDGELL C,PICKARD D,WAIN J,et al.Characterisation and distribution of a cryptic Salmonella typhi plasmid pHCM2[J].Plasmid,2002,47(3):159-171.
[16]梁莹,海荣,蔡虹.鼠疫菌3种常见质粒的结构特征分析[J].中国媒介生物学及控制杂志,2015,(1):33-36,40.
[17]SHEN X,WANG Q,XIA L,et al.Complete genome sequences of Yersinia pestis from natural foci in China[J].Journal of Bacteriology,2010,192(13):3551-3552.
[18]ZHGENTI E,JOHNSON S L,DAVENPORT K W,et al.Genome assemblies for 11 Yersinia pestis strains isolated in the caucasus region[J].Genome Announc,2015,3(5):e01030-15.
[19]JOHNSON S L,DALIGAULT H E,DAVENPORT K W,et al.Thirty-two complete genome assemblies of nine yersinia species,including Y.pestis,Y.pseudotuberculosis,and Y.enterocolitica:TABLE 1[J].Genome Announcements,2015,3(2):1-2.
[20]DU Y.Role of fraction 1 antigen of Yersinia pestis in inhibition of phagocytosis[J].Infection and Immunity,2002,70(3):1453-1460.
[21]KARLYSHEV A V,GALYOV E E,ABRAMOV V M,et al.Caf1Rgene and its role in the regulation of capsule formation of Y.pestis[J].FEBS Letters,1992,305(1):37-40.
[22]何电,董兴齐.鼠疫耶尔森氏菌质粒的结构和功能研究进展[J].地方病通报,2008,23(3):64-65,68.
[23]RUDOLPH A E,STUCKEY J A,ZHAO Y,et al.Expression,characterization,and mutagenesis of the Yersinia pestis murine toxin,a phospholipase D superfamily member[J].Journal of Biological Chemistry,1999,274(17):11824-11831.
[24]HINNEBUSCH B J.Role of yersinia murine toxin in survival of Yersinia pestis in the midgut of the flea vector[J].Science,2002,296(5568):733-735.
[25]MILLER D A,LUO L,HILLSON N,et al.Yersinia bactin synthetase:a four-protein assembly line producing the nonribosomal peptide/polyketide hybrid siderophore of Yersinia pestis.Chemistry&Biology,2002,9(3):333-344.
[26]范艳晓.鼠疫耶尔森氏菌降解其分泌的鼠毒素研究[D].合肥:安徽医科大学,2016.
[27]YOUNGREN B,RADNEDGE L,HU P,et al.A plasmid partition system of the P1-P7par family from the pMT1 virulence plasmid of Yersinia pestis[J].J Bacteriol,2000,182(14):3924-3928.
[28]HU P,ELLIOTT J,MCCREADY P,et al.Structural organization of virulence-associated plasmids of Yersinia pestis[J].J Bacteriol,1998,180(19):5192-5202.2018-11-14
[2]PERRY R D,FETHERSTON J D.Yersinia pestis:etiologic agent of plague[J].Clinical Microbiology Reviews,1997,10(1):35-66.
[3]KEIM P S,WAGNER D M.Humans and evolutionary and ecological forces shaped the phylogeography of recently emerged diseases[J].Nature Reviews Microbiology,2009,7(11):813-821.
[4]VOGLER A J,ANDRIANAIVOARIMANANA V,TELFER S,et al.Temporal phylogeography of Yersinia pestis in Madagascar:Insights into the Long-term maintenance of plague[J].PLoS Neglected Tropical Diseases,2017,11(9):e0005887.
[5]LESLIE R.Deadly plague epidemic rages in Madagascar 2017[N/OL].Science,2017-10-09[2018-11-01].http://www.sciencemag.org/news/2017/10/deadly-plague-epidemic-rages-madagascar.
[6]NGUYEN V K,PARRA-ROJAS C,HERNANDEZ-VARGAS E A.The 2017 plague outbreak in Madagascar:Data descriptions and epidemic modelling[J].Epidemics,2018,25:20-25.
[7]陈黎,张晔,倪俐,等.1996-2005年云南省红河州鼠疫监测结果分析[J].现代预防医学,2017,44(16):2900-2904.
[8]FERBER D M,BRUBAKER R R.Plasmids in Yersinia pestis[J].Infect Immun,1981,31:839-841.
[9]MCNALLY A,THOMSON N R,REUTER S,et al.Add,stir and reduce:Yersinia spp.as model bacteria for pathogen evolution[J].Nature Reviews Microbiology,2016,14(3):177-190.
[10]倪斌.鼠疫耶尔森菌质粒缺失株的构建及其致病相关的研究[D].西安:西北农林科技大学,2008.
[11]PRENTICE M B,JAMES K D,PARKHILL J,et al.Yersinia pestis pfra shows biovar-specific differences and recent common ancestry with a salmonella enterica serovar typhi plasmid[J].Journal of Bacteriology,2001,183(8):2586-2594.
[12]GOLUBOV A,NEUBAUER H,NOLTING C,et al.Structural organization of the pfra virulence-associated plasmid of rhamnose-positive Yersinia pestis[J].Infection and Immunity,2004,72(10):5613-5621.
[13]LINDLER L E,PLANO G V,BURLAND V,et al.Complete DNA sequence and detailed analysis of the Yersinia pestis KIM5 plasmid encoding murine toxin and capsular antigen[J].Infect Immun,1998,66:5731-5742.
[14]SONG Y.Complete genome sequence of Yersinia pestis strain91001,an isolate avirulent to humans[J].DNA Research,2004,11(3):179-197.
[15]KIDGELL C,PICKARD D,WAIN J,et al.Characterisation and distribution of a cryptic Salmonella typhi plasmid pHCM2[J].Plasmid,2002,47(3):159-171.
[16]梁莹,海荣,蔡虹.鼠疫菌3种常见质粒的结构特征分析[J].中国媒介生物学及控制杂志,2015,(1):33-36,40.
[17]SHEN X,WANG Q,XIA L,et al.Complete genome sequences of Yersinia pestis from natural foci in China[J].Journal of Bacteriology,2010,192(13):3551-3552.
[18]ZHGENTI E,JOHNSON S L,DAVENPORT K W,et al.Genome assemblies for 11 Yersinia pestis strains isolated in the caucasus region[J].Genome Announc,2015,3(5):e01030-15.
[19]JOHNSON S L,DALIGAULT H E,DAVENPORT K W,et al.Thirty-two complete genome assemblies of nine yersinia species,including Y.pestis,Y.pseudotuberculosis,and Y.enterocolitica:TABLE 1[J].Genome Announcements,2015,3(2):1-2.
[20]DU Y.Role of fraction 1 antigen of Yersinia pestis in inhibition of phagocytosis[J].Infection and Immunity,2002,70(3):1453-1460.
[21]KARLYSHEV A V,GALYOV E E,ABRAMOV V M,et al.Caf1Rgene and its role in the regulation of capsule formation of Y.pestis[J].FEBS Letters,1992,305(1):37-40.
[22]何电,董兴齐.鼠疫耶尔森氏菌质粒的结构和功能研究进展[J].地方病通报,2008,23(3):64-65,68.
[23]RUDOLPH A E,STUCKEY J A,ZHAO Y,et al.Expression,characterization,and mutagenesis of the Yersinia pestis murine toxin,a phospholipase D superfamily member[J].Journal of Biological Chemistry,1999,274(17):11824-11831.
[24]HINNEBUSCH B J.Role of yersinia murine toxin in survival of Yersinia pestis in the midgut of the flea vector[J].Science,2002,296(5568):733-735.
[25]MILLER D A,LUO L,HILLSON N,et al.Yersinia bactin synthetase:a four-protein assembly line producing the nonribosomal peptide/polyketide hybrid siderophore of Yersinia pestis.Chemistry&Biology,2002,9(3):333-344.
[26]范艳晓.鼠疫耶尔森氏菌降解其分泌的鼠毒素研究[D].合肥:安徽医科大学,2016.
[27]YOUNGREN B,RADNEDGE L,HU P,et al.A plasmid partition system of the P1-P7par family from the pMT1 virulence plasmid of Yersinia pestis[J].J Bacteriol,2000,182(14):3924-3928.
[28]HU P,ELLIOTT J,MCCREADY P,et al.Structural organization of virulence-associated plasmids of Yersinia pestis[J].J Bacteriol,1998,180(19):5192-5202.2018-11-14
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