全自动磁微粒化学发光法快速筛查呋喃类药物残留
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摘要
文章通过开发硝基呋喃类药物磁微粒化学发光试剂盒,建立了以磁微粒作为固相载体、化学发光作为检测信号的硝基呋喃类药物残留快速检测方法。结合全自动化学发光仪,用磁微粒化学发光酶联免疫法(MCLIA)同时对4种硝基呋喃类药物(呋喃西林代谢物(SEM)、呋喃唑酮代谢物(AOZ)、呋喃它酮代谢物(AMOZ)和呋喃妥因代谢物(AHD))在不同来源的鸡肉样、鱼样中的残留量进行定量检测,并与基于超高效液相色谱—三重四极杆串联质谱联用仪的国家标准方法的检测结果进行了对比。共检测鱼肉、猪肉、鸡肉和贝类样品等共计78个样本,与国家标准方法检测结果的符合率在98%以上。结果表明全自动微粒化学发光酶联免疫法具备有自动化程度高,多项目同时检测,检测效率高,检测结果准确可靠等优势,可用于硝基呋喃类药物残留的快速筛查。
In order to develop a magnetic particle chemiluminescence reagent kit for nitrofurans,a rapid detection method was established for the nitrofurans residues,which was based on magnetic particles as solid phase carrier and chemiluminescence as detection signal.The samples from different sources of chicken and fish,were been simultaneously quantitative detected for four nitrofurans residues as furacillin metabolites(SEM),furazolidone metabolites(AOZ),furan-tazone metabolites(AMOZ) and furantoin metabolites(AHD),by magnetic particle chemiluminescence enzyme-linked immunoassay(MCLIA) combined with full-automatic chemiluminescence apparatus,and then compared the results with the national standard method,which based on ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry.A total of 78 samples of fish,pork,chicken and shellfish were detected,and the coincidence rate,comparing the results with the national standard method's,was over 98%.The full-automatic particle chemiluminescence ELISA had the advantages of high automation,multi-item simultaneous detection,high detection efficiency and accurate and reliable detection results,while it could be used for rapid screening of nitrofurans residues.
In order to develop a magnetic particle chemiluminescence reagent kit for nitrofurans,a rapid detection method was established for the nitrofurans residues,which was based on magnetic particles as solid phase carrier and chemiluminescence as detection signal.The samples from different sources of chicken and fish,were been simultaneously quantitative detected for four nitrofurans residues as furacillin metabolites(SEM),furazolidone metabolites(AOZ),furan-tazone metabolites(AMOZ) and furantoin metabolites(AHD),by magnetic particle chemiluminescence enzyme-linked immunoassay(MCLIA) combined with full-automatic chemiluminescence apparatus,and then compared the results with the national standard method,which based on ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry.A total of 78 samples of fish,pork,chicken and shellfish were detected,and the coincidence rate,comparing the results with the national standard method's,was over 98%.The full-automatic particle chemiluminescence ELISA had the advantages of high automation,multi-item simultaneous detection,high detection efficiency and accurate and reliable detection results,while it could be used for rapid screening of nitrofurans residues.
引文
[1]祝伟霞,刘亚风,梁炜.动物性食品中硝基呋喃类药物残留检测研究进展[J].动物医学进展,2010,31(2):99-102.Zhu Weixia,Liu Yafeng,Liang Wei.Progress in the detection of nitrofuran residues in animal foods[J].Advances in Animal Medicine,2010,31(2):99-102.
[2]李耀平,林永辉,贾东芬,等.水产品中硝基呋喃代谢物残留快速检测新方法的研究[J].分析测试学报,2008,27(7):712-717.Li Yaoping,Lin Yonghui,Jia Dongfen,et al.Study on a new method for rapid detection of nitrofuran metabolite residues in aquatic products[J].Journal of Analysis and Testing,2008,27(7):712-717.
[3]宋宁宁.呋喃妥因代谢产物AHD单抗的制备及其快速检测技术的研究[D].北京:中国农业科学院,2012.Song Ningning.Study on the Preparation of Monoclonal Antibody Against Furantoin Metabolite AHD and Its Rapid Detection Technology[D].Beijing:Chinese Academy of Agricultural Sciences,2012.
[4]环境保护部.国家污染物环境健康风险名录--化学第二分册[M].北京:中国环境科学出版社,2011.Ministry of Environmental Protection.National List of Environmental Health Risks of Pollutants-VolumeⅡof Chemistry[M].Beijing:China Environmental Science Press,2011.
[5]何艳玲,刘敏,王丽哲.EP硝基呋喃类代谢物ELISA检测试剂盒:检测更快速、更准确[J].食品安全导刊,2010,21(5):36-37.He Yanling,Liu Min,Wang Lizhe.EP nitrofuran metabolite ELISA kit:faster and more accurate detection[J].Food Safety Guide,2010,21(5):36-37.
[6]涂勇,陈玉清,赵豫琴.磁性分离酶标免疫分析法测定孕酮、睾酮和垂体泌乳素[J].标记免疫分析与临床,1999(2):132-133.Tu Yong,Chen Yuqing,Zhao Yuqin.Determination of progesterone,testosterone and pituitary prolactin by magnetic separation enzyme-labeled immunoassay[J].Labeled Immunoassay and Clinical Practice,1999(2):132-133.
[7]Cooper K M,Caddell A,Elliott C T,et al.Production and characterisation of polyclonal antibodies to a derivative of3-amino-2-oxazolidinone,a metabolite of the nitrofuran furazolidone[J].Analytica Chimica Acta,2004,520(1):79-86.
[8]曾磊,周常义,苏国成.呋喃唑酮代谢物人工抗原的合成及半抗原偶联比的测定[J].安徽农业科学,2013,41(3):961-962,1022.Zeng Lei,Zhou Changyi,Su Guocheng.Synthesis of artificial antigen of furazolidone metabolite and determination of hapten coupling ratio[J].Anhui Agricultural Science,2013,41(3):961-962,1022.
[9]Park J,An K,Hwang Y,et al.Ultra-large-scale syntheses of monodisperse nanocrystals[J].Nature Materials,2004,3(12):891-895.
[10]蒋泽权,宋晟,窦红静,等.配体交换法制备水中高分散稳定性的多羧基修饰Fe3O4纳米颗粒[J].高等学校化学学报,2012,33(12):2609-2616.Jiang Zequan,Song Sheng,Dou Hongjing,et al.Preparation of highly dispersible and stable polycarboxyl modified Fe3O4nanoparticles in water by ligand exchange[J].Journal of Chemistry of Colleges and Universities,2012,33(12):2609-2616.
[11]冯燕.SMCC法抗体定向偶联技术的优化与应用研究[D].武汉:华中科技大学,2011.Feng Yan.The Optimization and Application of SMCC Antibody Directional Coupling[D].Wuhan:Huazhong University of Science and Technology,2011.
[12]蒋原.HPLC/MS/MS测定兽药残留及其在螯虾中消除规律的研究[D].南京:南京农业大学,2005.Jiang Yuan.Determination of Veterinary Drug Residues and Their Elimination in Crayfish by HPLC/MS/MS[D].Nanjing:Nanjing Agricultural University,2005.
[2]李耀平,林永辉,贾东芬,等.水产品中硝基呋喃代谢物残留快速检测新方法的研究[J].分析测试学报,2008,27(7):712-717.Li Yaoping,Lin Yonghui,Jia Dongfen,et al.Study on a new method for rapid detection of nitrofuran metabolite residues in aquatic products[J].Journal of Analysis and Testing,2008,27(7):712-717.
[3]宋宁宁.呋喃妥因代谢产物AHD单抗的制备及其快速检测技术的研究[D].北京:中国农业科学院,2012.Song Ningning.Study on the Preparation of Monoclonal Antibody Against Furantoin Metabolite AHD and Its Rapid Detection Technology[D].Beijing:Chinese Academy of Agricultural Sciences,2012.
[4]环境保护部.国家污染物环境健康风险名录--化学第二分册[M].北京:中国环境科学出版社,2011.Ministry of Environmental Protection.National List of Environmental Health Risks of Pollutants-VolumeⅡof Chemistry[M].Beijing:China Environmental Science Press,2011.
[5]何艳玲,刘敏,王丽哲.EP硝基呋喃类代谢物ELISA检测试剂盒:检测更快速、更准确[J].食品安全导刊,2010,21(5):36-37.He Yanling,Liu Min,Wang Lizhe.EP nitrofuran metabolite ELISA kit:faster and more accurate detection[J].Food Safety Guide,2010,21(5):36-37.
[6]涂勇,陈玉清,赵豫琴.磁性分离酶标免疫分析法测定孕酮、睾酮和垂体泌乳素[J].标记免疫分析与临床,1999(2):132-133.Tu Yong,Chen Yuqing,Zhao Yuqin.Determination of progesterone,testosterone and pituitary prolactin by magnetic separation enzyme-labeled immunoassay[J].Labeled Immunoassay and Clinical Practice,1999(2):132-133.
[7]Cooper K M,Caddell A,Elliott C T,et al.Production and characterisation of polyclonal antibodies to a derivative of3-amino-2-oxazolidinone,a metabolite of the nitrofuran furazolidone[J].Analytica Chimica Acta,2004,520(1):79-86.
[8]曾磊,周常义,苏国成.呋喃唑酮代谢物人工抗原的合成及半抗原偶联比的测定[J].安徽农业科学,2013,41(3):961-962,1022.Zeng Lei,Zhou Changyi,Su Guocheng.Synthesis of artificial antigen of furazolidone metabolite and determination of hapten coupling ratio[J].Anhui Agricultural Science,2013,41(3):961-962,1022.
[9]Park J,An K,Hwang Y,et al.Ultra-large-scale syntheses of monodisperse nanocrystals[J].Nature Materials,2004,3(12):891-895.
[10]蒋泽权,宋晟,窦红静,等.配体交换法制备水中高分散稳定性的多羧基修饰Fe3O4纳米颗粒[J].高等学校化学学报,2012,33(12):2609-2616.Jiang Zequan,Song Sheng,Dou Hongjing,et al.Preparation of highly dispersible and stable polycarboxyl modified Fe3O4nanoparticles in water by ligand exchange[J].Journal of Chemistry of Colleges and Universities,2012,33(12):2609-2616.
[11]冯燕.SMCC法抗体定向偶联技术的优化与应用研究[D].武汉:华中科技大学,2011.Feng Yan.The Optimization and Application of SMCC Antibody Directional Coupling[D].Wuhan:Huazhong University of Science and Technology,2011.
[12]蒋原.HPLC/MS/MS测定兽药残留及其在螯虾中消除规律的研究[D].南京:南京农业大学,2005.Jiang Yuan.Determination of Veterinary Drug Residues and Their Elimination in Crayfish by HPLC/MS/MS[D].Nanjing:Nanjing Agricultural University,2005.
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