基于微流控技术的纳升级蛋白质结晶筛选方法的研究进展
|
摘要
X射线晶体学技术是目前蛋白质结构测定中最主要的方法。为了得到满足衍射要求的高质量蛋白质晶体,研究者通常耗费大量试剂和样品进行大规模结晶条件筛选。微流控技术通过对超微量流体的操纵,可大幅降低在蛋白质结晶筛选中蛋白样品的消耗。本文依据结晶方法,分别介绍了基于微批量法、蒸气扩散法、自由界面扩散法和透析法的微流控蛋白质结晶筛选方法的研究进展。
X-ray crystallography is the major approach in the determination of protein structure. To obtain high quality protein crystals that meet the diffraction requirements,researchers usually spend large amounts of reagents and samples for large-scale crystallization condition screening. Microfluidic technology can greatly reduce the consumption of protein samples in protein crystallization screening by manipulating ultramicro amounts of fluids. In this review,the recent progress in microfluidic protein crystallization screening methods based on the microbatch,vapor diffusion,free-interface diffusion and dialysis methods is introduced according to the difference in crystallization methods.
X-ray crystallography is the major approach in the determination of protein structure. To obtain high quality protein crystals that meet the diffraction requirements,researchers usually spend large amounts of reagents and samples for large-scale crystallization condition screening. Microfluidic technology can greatly reduce the consumption of protein samples in protein crystallization screening by manipulating ultramicro amounts of fluids. In this review,the recent progress in microfluidic protein crystallization screening methods based on the microbatch,vapor diffusion,free-interface diffusion and dialysis methods is introduced according to the difference in crystallization methods.
引文
1 Mcpherson A.Methods,2004,34(3):254-265
2 Liao M,Cao E,Julius D,Cheng Y.Nature,2013,504(7478):107
3 Merk A,Bartesaghi A,Banerjee S,Falconieri V,Rao P,Davis M I,Pragani R,Boxer M B,Earl L A,Milne J L S,Subramaniam S.Cell,2016,165(7):1698-1707
4 Gorrec F.Drug Discovery Today,2014,19(10):1505-1507
5 Khurshid S,Saridakis E,Govada L,Chayen N E.Nat.Protoc.,2014,9(7):1621-1633
6 Li L,Ismagilov R F.Annu.Rev.Biophys.,2010,39(39):139-158
7 Newby Z E R,O'Connell J D I,Gruswitz F,Hays F A,Harries W E C,Harwood I M,Ho J D,Lee J K,Savage D F,Miercke L J W,Stroud R M.Nat.Protoc.,2009,4(5):619-637
8 Sauter C,Dhouib K,Lorber B.Cryst.Growth Des.,2007,7(11):2247-2250
9 Leng J,Salmon J.Lab Chip,2009,9(1):24-34
10 ZHU Li-Na,ZHU Ying,FANG Qun.Chem.J.Chinese U.,2014,35(1):1-11朱丽娜,祝莹,方群.高等学校化学学报,2014,35(1):1-11
11 Juarez-Martinez G.Encyclopedia of Nanotechnology.Dordrecht,the Netherlands:Springer-Verlag Press,2016:1235-1248
12 Shi H,Xiao Y,Ferguson S,Huang X,Wang N,Hao H.Lab Chip,2017,17(13):2167-2185
13 Gavira J A.Arch.Biochem.Biophys.,2016,602(SI):3-11
14 Chayen N E.Acta Crystallogr.D,1998,54(1):8-15
15 Chayen N E,Shaw Stewart P D,Maeder D L,Blow D M.J.Appl.Cryst.,1990,23(4):297-302
16 Chayen N E,Shaw Stewart P D.J.Cryst.Growth,1992,122(1-4):176-180
17 D'Arcy A,Mac Sweeney A,Stihle M,Haber A.Acta Crystallogr.D,2003,59(2):396-399
18 D'Arcy A,Elmore C,Stihle M,Johnston J E.J.Cryst.Growth,1996,168(1-4):175-180
19 Zheng B,Roach L S,Ismagilov R F.J.Am.Chem.Soc.,2003,125(37):11170-11171
20 Thorsen T,Roberts R W,Arnold F H,Quake S R.Phys.Rev.Lett.,2001,86(18):4163-4166
21 Gerdts C J,Stahl G L,Napuli A,Staker B,Abendroth J,Edwards T E,Myler P,Van Voorhis W,Nollert P,Stewart L J.J.Appl.Crystallogr.,2010,43(5):1078-1083
22 Gerdts C J,Elliott M,Lovell S,Mixon M B,Napuli A J,Staker B L,Nollert P,Stewart L.Acta Crystallogr.D,2008,64(11):1116-1122
23 Pham N,Radajewski D,Round A,Brennich M,Pernot P,Biscans B,Bonnete F.Anal.Chem.,2017,89(4):2282-2287
24 Zheng B,Tice J D,Roach L S,Ismagilov R F.Angew.Chem.Int.Edit.,2004,43(19):2508-2511
25 Sun M,Fang Q.Lab Chip,2010,10(21):2864-2868
26 Zheng B,Ismagilov R F.Angew.Chem.Int.Edit.,2005,44(17):2520-2523
27 Chen D L,Ismagilov R F.Curr.Opin.Chem.Biol.,2006,10(3):226-231
28 Du W,Sun M,Gu S,Zhu Y,Fang Q.Anal.Chem.,2010,82(23):9941-9947
29 Du W,Li L,Nichols K P,Ismagilov R F.Lab Chip,2009,9(16):2286-2292
30 Zhou X,Lau L,Lam W W L,Au S W N,Zheng B.Anal.Chem.,2007,79(13):4924-4930
31 Li L,Du W,Ismagilov R F.J.Am.Chem.Soc.,2010,132(1):106-111
32 Li L,Du W,Ismagilov R F.J.Am.Chem.Soc.,2010,132(1):112-119
33 Li G,Chen Q,Li J,Hu X,Zhao J.Anal.Chem.,2010,82(11):4362-4369
34 Wang L,Sun K,Hu X,Li G,Jin Q,Zhao J.Sens.Actuators B,2015,219:105-111
35 Yang C,Liu Y,Di Y,Xu Z.Microfluid.Nanofluid,2015,18(3):493-501
36 Yang C,Wu Y,Xu Z,Wang J.Lab Chip,2011,11(19):3305-3312
37 Yang C,Xu Z,Lee A P,Wang J.Lab Chip,2013,13(14):2815-2820
38 Abdallah B G,Roy-Chowdhury S,Fromme R,Fromme P,Ros A.Cryst.Growth Des.,2016,16(4):2074-2082
39 Zhu Y,Zhu L,Guo R,Cui H,Ye S,Fang Q.Sci.Rep.,2014,4:5046
40 Zhu Y,Zhang Y,Cai L,Fang Q.Anal.Chem.,2013,85(14):6723-6731
41 Villasenor A G,Wong A,Shao A,Garg A,Kuglstatter A,Harris S F.Acta Crystallogr.D,2010,66(5):568-576
42 Yin X,Scalia A,Leroy L,Cuttitta C M,Polizzo G M,Ericson D L,Roessler C G,Campos O,Ma M Y,Agarwal R,Jackimowicz R,Allaire M,Orville A M,Sweet R M,Soares A S.Acta Crystallogr.D,2014,70(5):1177-1189
43 Teplitsky E,Joshi K,Ericson D L,Scalia A,Mullen J D,Sweet R M,Soares A S.J.Struct.Biol.,2015,191(1):49-58
44 Liang Y,Zhu L,Gao J,Zhao H,Zhu Y,Ye S,Fang Q.ACS Appl.Mater.Interfaces,2017,9(13):11837-11845
45 Shim J,Cristobal G,Link D R,Thorsen T,Fraden S.Cryst.Growth Des.,2007,7(11):2192-2194
46 Mcpherson A.Crystallization of Biological Macromolecules,Plainview,USA:Cold Spring Harbor Lab.Press,1999
47 Hansen C L,Skordalakes E,Berger J M,Quake S R.Proc.Natl.Acad.Sci.USA,2002,99(26):16531-16536
48 Unger M A,Chou H P,Thorsen T,Scherer A,Quake S R.Science,2000,288(5463):113-116
49 Maeki M,Yamazaki S,Pawate A S,Ishida A,Tani H,Yamashita K,Sugishima M,Watanabe K,Tokeshi M,Kenis P JA,Miyazaki M.Cryst Eng Comm,2016,18(40):7722-7727
50 Hansen C L,Classen S,Berger J M,Quake S R.J.Am.Chem.Soc.,2006,128(10):3142-3143
51 Hansen C L,Sommer M,Quake S R.Proc.Natl.Acad.Sci.USA,2004,101(40):14431-14436
52 Lau B T C,Baitz C A,Dong X P,Hansen C L.J.Am.Chem.Soc.,2007,129(3):454-455
53 Feuerborn A,Prastowo A,Cook P R,Walsh E.Lab Chip,2015,15(18):3766-3775
54 Aller P,Sanchez-Weatherby J,Foadi J,Winter G,Lobley C M C,Axford D,Ashton A W,Bellini D,Brandao-Neto J,Culurgioni S,Douangamath A,Duman R,Evans G,Fisher S,Flaig R,Hall D R,Lukacik P,Mazzorana M,McAuley KE,Mykhaylyk V,Owen R L,Paterson N G,Romano P,Sandy J,Sorensen T,Von Delft F,Wagner A,Warren A,Williams M,Stuart D I,Walsh M A.Methods Mol.Biol.,2015,1261:233-253
55 Yadav M K,Gerdts C J,Sanishvili R,Smith W W,Roach L S,Ismagilov R F,Kuhn P,Stevens R C.J.Appl.Crystallogr.,2005,38(6):900-905
56 Lyubimov A Y,Murray T D,Koehl A,Araci I E,Uervirojnangkoorn M,Zeldin O B,Cohen A E,Soltis S M,Baxter E L,Brewster A S,Sauter N K,Brunger A T,Berger J M.Acta Crystallogr.D,2015,71(4):928-940
57 Ng J D,Clark P J,Stevens R C,Kuhn P.Acta Crystallogr.D,2008,64(2):189-197
58 Pinker F,Brun M,Morin P,Deman A,Chateaux J,Olieric V,Stirnimann C,Lorber B,Terrier N,Ferrigno R,Sauter C.Cryst.Growth Des.,2013,13(8):3333-3340
59 Broecker J,Klingel V,Ou W,Balo A R,Kissick D J,Ogata C M,Kuo A,Ernst O P.Cryst.Growth Des.,2016,16(11):6318-6326
60 Broecker J,Morizumi T,Ou W,Klingel V,Kuo A,Kissick D J,Ishchenko A,Lee M,Xu S,Makarov O,Cherezov V,Ogata C M,Ernst O P.Nat.Protoc.,2018,13(2):260-291
61 LIANG Yi-Ran,ZHU Ying,FANG Qun.Chinese Journal of Chromatography,2016,34(12):1137-1144梁翼然,祝莹,方群.色谱,2016,34(12):1137-1144
2 Liao M,Cao E,Julius D,Cheng Y.Nature,2013,504(7478):107
3 Merk A,Bartesaghi A,Banerjee S,Falconieri V,Rao P,Davis M I,Pragani R,Boxer M B,Earl L A,Milne J L S,Subramaniam S.Cell,2016,165(7):1698-1707
4 Gorrec F.Drug Discovery Today,2014,19(10):1505-1507
5 Khurshid S,Saridakis E,Govada L,Chayen N E.Nat.Protoc.,2014,9(7):1621-1633
6 Li L,Ismagilov R F.Annu.Rev.Biophys.,2010,39(39):139-158
7 Newby Z E R,O'Connell J D I,Gruswitz F,Hays F A,Harries W E C,Harwood I M,Ho J D,Lee J K,Savage D F,Miercke L J W,Stroud R M.Nat.Protoc.,2009,4(5):619-637
8 Sauter C,Dhouib K,Lorber B.Cryst.Growth Des.,2007,7(11):2247-2250
9 Leng J,Salmon J.Lab Chip,2009,9(1):24-34
10 ZHU Li-Na,ZHU Ying,FANG Qun.Chem.J.Chinese U.,2014,35(1):1-11朱丽娜,祝莹,方群.高等学校化学学报,2014,35(1):1-11
11 Juarez-Martinez G.Encyclopedia of Nanotechnology.Dordrecht,the Netherlands:Springer-Verlag Press,2016:1235-1248
12 Shi H,Xiao Y,Ferguson S,Huang X,Wang N,Hao H.Lab Chip,2017,17(13):2167-2185
13 Gavira J A.Arch.Biochem.Biophys.,2016,602(SI):3-11
14 Chayen N E.Acta Crystallogr.D,1998,54(1):8-15
15 Chayen N E,Shaw Stewart P D,Maeder D L,Blow D M.J.Appl.Cryst.,1990,23(4):297-302
16 Chayen N E,Shaw Stewart P D.J.Cryst.Growth,1992,122(1-4):176-180
17 D'Arcy A,Mac Sweeney A,Stihle M,Haber A.Acta Crystallogr.D,2003,59(2):396-399
18 D'Arcy A,Elmore C,Stihle M,Johnston J E.J.Cryst.Growth,1996,168(1-4):175-180
19 Zheng B,Roach L S,Ismagilov R F.J.Am.Chem.Soc.,2003,125(37):11170-11171
20 Thorsen T,Roberts R W,Arnold F H,Quake S R.Phys.Rev.Lett.,2001,86(18):4163-4166
21 Gerdts C J,Stahl G L,Napuli A,Staker B,Abendroth J,Edwards T E,Myler P,Van Voorhis W,Nollert P,Stewart L J.J.Appl.Crystallogr.,2010,43(5):1078-1083
22 Gerdts C J,Elliott M,Lovell S,Mixon M B,Napuli A J,Staker B L,Nollert P,Stewart L.Acta Crystallogr.D,2008,64(11):1116-1122
23 Pham N,Radajewski D,Round A,Brennich M,Pernot P,Biscans B,Bonnete F.Anal.Chem.,2017,89(4):2282-2287
24 Zheng B,Tice J D,Roach L S,Ismagilov R F.Angew.Chem.Int.Edit.,2004,43(19):2508-2511
25 Sun M,Fang Q.Lab Chip,2010,10(21):2864-2868
26 Zheng B,Ismagilov R F.Angew.Chem.Int.Edit.,2005,44(17):2520-2523
27 Chen D L,Ismagilov R F.Curr.Opin.Chem.Biol.,2006,10(3):226-231
28 Du W,Sun M,Gu S,Zhu Y,Fang Q.Anal.Chem.,2010,82(23):9941-9947
29 Du W,Li L,Nichols K P,Ismagilov R F.Lab Chip,2009,9(16):2286-2292
30 Zhou X,Lau L,Lam W W L,Au S W N,Zheng B.Anal.Chem.,2007,79(13):4924-4930
31 Li L,Du W,Ismagilov R F.J.Am.Chem.Soc.,2010,132(1):106-111
32 Li L,Du W,Ismagilov R F.J.Am.Chem.Soc.,2010,132(1):112-119
33 Li G,Chen Q,Li J,Hu X,Zhao J.Anal.Chem.,2010,82(11):4362-4369
34 Wang L,Sun K,Hu X,Li G,Jin Q,Zhao J.Sens.Actuators B,2015,219:105-111
35 Yang C,Liu Y,Di Y,Xu Z.Microfluid.Nanofluid,2015,18(3):493-501
36 Yang C,Wu Y,Xu Z,Wang J.Lab Chip,2011,11(19):3305-3312
37 Yang C,Xu Z,Lee A P,Wang J.Lab Chip,2013,13(14):2815-2820
38 Abdallah B G,Roy-Chowdhury S,Fromme R,Fromme P,Ros A.Cryst.Growth Des.,2016,16(4):2074-2082
39 Zhu Y,Zhu L,Guo R,Cui H,Ye S,Fang Q.Sci.Rep.,2014,4:5046
40 Zhu Y,Zhang Y,Cai L,Fang Q.Anal.Chem.,2013,85(14):6723-6731
41 Villasenor A G,Wong A,Shao A,Garg A,Kuglstatter A,Harris S F.Acta Crystallogr.D,2010,66(5):568-576
42 Yin X,Scalia A,Leroy L,Cuttitta C M,Polizzo G M,Ericson D L,Roessler C G,Campos O,Ma M Y,Agarwal R,Jackimowicz R,Allaire M,Orville A M,Sweet R M,Soares A S.Acta Crystallogr.D,2014,70(5):1177-1189
43 Teplitsky E,Joshi K,Ericson D L,Scalia A,Mullen J D,Sweet R M,Soares A S.J.Struct.Biol.,2015,191(1):49-58
44 Liang Y,Zhu L,Gao J,Zhao H,Zhu Y,Ye S,Fang Q.ACS Appl.Mater.Interfaces,2017,9(13):11837-11845
45 Shim J,Cristobal G,Link D R,Thorsen T,Fraden S.Cryst.Growth Des.,2007,7(11):2192-2194
46 Mcpherson A.Crystallization of Biological Macromolecules,Plainview,USA:Cold Spring Harbor Lab.Press,1999
47 Hansen C L,Skordalakes E,Berger J M,Quake S R.Proc.Natl.Acad.Sci.USA,2002,99(26):16531-16536
48 Unger M A,Chou H P,Thorsen T,Scherer A,Quake S R.Science,2000,288(5463):113-116
49 Maeki M,Yamazaki S,Pawate A S,Ishida A,Tani H,Yamashita K,Sugishima M,Watanabe K,Tokeshi M,Kenis P JA,Miyazaki M.Cryst Eng Comm,2016,18(40):7722-7727
50 Hansen C L,Classen S,Berger J M,Quake S R.J.Am.Chem.Soc.,2006,128(10):3142-3143
51 Hansen C L,Sommer M,Quake S R.Proc.Natl.Acad.Sci.USA,2004,101(40):14431-14436
52 Lau B T C,Baitz C A,Dong X P,Hansen C L.J.Am.Chem.Soc.,2007,129(3):454-455
53 Feuerborn A,Prastowo A,Cook P R,Walsh E.Lab Chip,2015,15(18):3766-3775
54 Aller P,Sanchez-Weatherby J,Foadi J,Winter G,Lobley C M C,Axford D,Ashton A W,Bellini D,Brandao-Neto J,Culurgioni S,Douangamath A,Duman R,Evans G,Fisher S,Flaig R,Hall D R,Lukacik P,Mazzorana M,McAuley KE,Mykhaylyk V,Owen R L,Paterson N G,Romano P,Sandy J,Sorensen T,Von Delft F,Wagner A,Warren A,Williams M,Stuart D I,Walsh M A.Methods Mol.Biol.,2015,1261:233-253
55 Yadav M K,Gerdts C J,Sanishvili R,Smith W W,Roach L S,Ismagilov R F,Kuhn P,Stevens R C.J.Appl.Crystallogr.,2005,38(6):900-905
56 Lyubimov A Y,Murray T D,Koehl A,Araci I E,Uervirojnangkoorn M,Zeldin O B,Cohen A E,Soltis S M,Baxter E L,Brewster A S,Sauter N K,Brunger A T,Berger J M.Acta Crystallogr.D,2015,71(4):928-940
57 Ng J D,Clark P J,Stevens R C,Kuhn P.Acta Crystallogr.D,2008,64(2):189-197
58 Pinker F,Brun M,Morin P,Deman A,Chateaux J,Olieric V,Stirnimann C,Lorber B,Terrier N,Ferrigno R,Sauter C.Cryst.Growth Des.,2013,13(8):3333-3340
59 Broecker J,Klingel V,Ou W,Balo A R,Kissick D J,Ogata C M,Kuo A,Ernst O P.Cryst.Growth Des.,2016,16(11):6318-6326
60 Broecker J,Morizumi T,Ou W,Klingel V,Kuo A,Kissick D J,Ishchenko A,Lee M,Xu S,Makarov O,Cherezov V,Ogata C M,Ernst O P.Nat.Protoc.,2018,13(2):260-291
61 LIANG Yi-Ran,ZHU Ying,FANG Qun.Chinese Journal of Chromatography,2016,34(12):1137-1144梁翼然,祝莹,方群.色谱,2016,34(12):1137-1144
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |