厦门地区低致病性禽流感病毒流行病学调查与分析
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摘要
目的了解厦门地区家禽低致病性禽流感病毒的流行情况。方法本研究选取禽流感病毒检出率最高的冬春季节,从厦门市几家主要活禽交易市场采集家禽棉拭子及环境样品,用荧光定量PCR方法进行低致病性禽流感病毒的检测和分型鉴定。结果从534份样品中,检测出低致病性禽流感病毒阳性216份,检出率为40.4%。本地低致病禽流感病毒的HA亚型至少6种,检出率由高到低依次为H9、H6、H11、H12、H1、H3;NA亚型至少5种,检出率由高到低依次为N2、N6、N3、N8、N9。结论活禽交易市场家禽中存在不同亚型混合感染情况,尤其以水禽最明显,可同时检出多种亚型。提示今后厦门的疫病防控须加大对水禽的监测及交易市场的管理和消毒。
This study engaged in epidemiological surveillance of low pathogenic avian influenza A viruses in poultry of Xiamen. From winter to spring, cloacal swab samples were collected from poultry, which were from main live-poultry markets of different districts in Xiamen. Real-time PCR method were using for detection and subtype identification. The 216 strains of AIVs were detected from 534 cloacal swab samples, with a detection rate of 40.4%. These strains of AIV belonged to 6 HA subtypes and 5 NA subtypes, the detection rate of high to low were H9, H6, H11, H12, H1, H3 and N2, N6, N3, N8, N9. Mixed infections were much higher in waterfowl origin, different subtypes were detected in live-poultry markets, indicating that monitoring of avian influenza virus in domestic waterfowls should be reinforced an standardized administration and thoroughly disinfection were needed in future.
This study engaged in epidemiological surveillance of low pathogenic avian influenza A viruses in poultry of Xiamen. From winter to spring, cloacal swab samples were collected from poultry, which were from main live-poultry markets of different districts in Xiamen. Real-time PCR method were using for detection and subtype identification. The 216 strains of AIVs were detected from 534 cloacal swab samples, with a detection rate of 40.4%. These strains of AIV belonged to 6 HA subtypes and 5 NA subtypes, the detection rate of high to low were H9, H6, H11, H12, H1, H3 and N2, N6, N3, N8, N9. Mixed infections were much higher in waterfowl origin, different subtypes were detected in live-poultry markets, indicating that monitoring of avian influenza virus in domestic waterfowls should be reinforced an standardized administration and thoroughly disinfection were needed in future.
引文
[1] Fouchier RA, Munster V, Wallensten A, et al. Characterization of a novel influenza A virus hemagglutinin subtype(H16) obtained from black-headed gulls[J]. J Virol, 2005, 79(5): 2814-2822. DOI:10.1128/jvi.79.5.2814-2822.2005
[2] Ying W, Yan W, Boris T, et al. Bat-derived influenza-like viruses H17N10 and H18N11[J]. Trends Microbiol, 2014, 22(4): 183-191. DOI:10.1016/j.tim.2014.01.010
[3] 郭元吉, 李建国, 陈小雯, 等. 禽H9N2亚型流感病毒能感染人的发现[J]. 中华实验和临床病毒学, 1999, 13(2): 105-108. DOI:10.3760/cma.j.issn.1003-9279.1999.02.001
[4] Zhang T, Bi Y, Tian H, et al. Human infection with influenza virus A(H10N8) from live poultry markets,China,2014[J]. Emerg Infect Dis, 2014, 20(12): 2076-2079. DOI:10.3201/eid2012.140911
[5] Gao R, Cao B, Hu Y, et al. Human infection with a novel avian-origin influenza A(H7N9) virus[J]. Engl J Med. 2013, 368(20): 1888-1897. DOI:10.1056/NEJMoa1304459
[6] Yuan J, Zhang L, Kan X, et al. Origin and molecular characteristics of a novel 2013 avian influenza A(H6N1) virus causing human infection in Taiwan[J]. Clin Infect Dis an official publication of the Infectious Diseases Society of America. 2013, 57(9): 1367-1368. DOI:10.1093/cid/cit479
[7] 赵国, 刘晓文, 钱忠明,等. 2002-2009年中国华东地区家禽低致病性禽流感的病原学检测与分析[J]. 中国农业科学, 2011, 44(1): 153-159. DOI:10.3864/j.issn.0578-1752.2011.01.018
[8] 彭宜, 张伟, 薛峰, 等. 2006-2008年对华东地区家禽不同HA亚型低致病性禽流感的病原学监测[J]. 中国人兽共患病学报, 2009, 25(2): 119-121.
[9] 赵国, 赵坤坤, 鹿欣伦, 等. 华东地区家禽低致病性禽流感的病原学检测与带毒情况的分析[J]. 畜牧兽医学报, 2010, 41(9): 1133-1137.
[10] 彭国文, 倪汉忠, 刘少梅, 等. 广东省1998～2000年人群禽流行性感冒病原学监测[J]. 中华流行病学杂志, 2002, 23(2): 154. DOI:10.3760/j.issn:0254-6450.2002.02.024
[11] 游胜, 冯秀, 刘晓青,等. 江西省首例人感染H9N2禽流感病例流行病学调查[J]. 现代预防医学, 2017, 44(5): 782-784.
[12] 刘晓蕾, 袁洁, 欧新华,等. 长沙市首例人感染H9N2禽流感病毒的分离及基因特征分析[J]. 现代预防医学, 2016, 43(17): 3225-3231.
[13] 杨磊, 杜训波, 张晓春,等. 成都市首例人感染H9N2禽流感病例调查与分析[J]. 中国人兽共患病学报, 2017, 33(3): 245-249. DOI:10.3969/j.issn.1002-2694.2017.03.010
[14] 罗春蕊, 赵晓南, 宁德明,等. 云南省首例人感染H9N2禽流感病例发现与应对[J]. 中国人兽共患病学报, 2017, 33(3): 241-244. DOI:10.3969/j.issn.1002-2694.2009.02.007
[15] 王玥莲, 李继科, 王林,等. 2016年四川省首例人感染禽流感H9N2危重症诊治抢救分析[J].中国呼吸与危重监护杂志, 2016, 15(4): 415-417. DOI:10.7507/1671-6205.2016098
[16] 辛丽, 白天, 周剑芳,等. 中国职业暴露人群感染H9N2 禽流感病毒血清学调查[J]. 疾病监测, 2015, 30 (5): 368-370. DOI:10.3784/j.issn.1003-9961.2015.05.007
[2] Ying W, Yan W, Boris T, et al. Bat-derived influenza-like viruses H17N10 and H18N11[J]. Trends Microbiol, 2014, 22(4): 183-191. DOI:10.1016/j.tim.2014.01.010
[3] 郭元吉, 李建国, 陈小雯, 等. 禽H9N2亚型流感病毒能感染人的发现[J]. 中华实验和临床病毒学, 1999, 13(2): 105-108. DOI:10.3760/cma.j.issn.1003-9279.1999.02.001
[4] Zhang T, Bi Y, Tian H, et al. Human infection with influenza virus A(H10N8) from live poultry markets,China,2014[J]. Emerg Infect Dis, 2014, 20(12): 2076-2079. DOI:10.3201/eid2012.140911
[5] Gao R, Cao B, Hu Y, et al. Human infection with a novel avian-origin influenza A(H7N9) virus[J]. Engl J Med. 2013, 368(20): 1888-1897. DOI:10.1056/NEJMoa1304459
[6] Yuan J, Zhang L, Kan X, et al. Origin and molecular characteristics of a novel 2013 avian influenza A(H6N1) virus causing human infection in Taiwan[J]. Clin Infect Dis an official publication of the Infectious Diseases Society of America. 2013, 57(9): 1367-1368. DOI:10.1093/cid/cit479
[7] 赵国, 刘晓文, 钱忠明,等. 2002-2009年中国华东地区家禽低致病性禽流感的病原学检测与分析[J]. 中国农业科学, 2011, 44(1): 153-159. DOI:10.3864/j.issn.0578-1752.2011.01.018
[8] 彭宜, 张伟, 薛峰, 等. 2006-2008年对华东地区家禽不同HA亚型低致病性禽流感的病原学监测[J]. 中国人兽共患病学报, 2009, 25(2): 119-121.
[9] 赵国, 赵坤坤, 鹿欣伦, 等. 华东地区家禽低致病性禽流感的病原学检测与带毒情况的分析[J]. 畜牧兽医学报, 2010, 41(9): 1133-1137.
[10] 彭国文, 倪汉忠, 刘少梅, 等. 广东省1998～2000年人群禽流行性感冒病原学监测[J]. 中华流行病学杂志, 2002, 23(2): 154. DOI:10.3760/j.issn:0254-6450.2002.02.024
[11] 游胜, 冯秀, 刘晓青,等. 江西省首例人感染H9N2禽流感病例流行病学调查[J]. 现代预防医学, 2017, 44(5): 782-784.
[12] 刘晓蕾, 袁洁, 欧新华,等. 长沙市首例人感染H9N2禽流感病毒的分离及基因特征分析[J]. 现代预防医学, 2016, 43(17): 3225-3231.
[13] 杨磊, 杜训波, 张晓春,等. 成都市首例人感染H9N2禽流感病例调查与分析[J]. 中国人兽共患病学报, 2017, 33(3): 245-249. DOI:10.3969/j.issn.1002-2694.2017.03.010
[14] 罗春蕊, 赵晓南, 宁德明,等. 云南省首例人感染H9N2禽流感病例发现与应对[J]. 中国人兽共患病学报, 2017, 33(3): 241-244. DOI:10.3969/j.issn.1002-2694.2009.02.007
[15] 王玥莲, 李继科, 王林,等. 2016年四川省首例人感染禽流感H9N2危重症诊治抢救分析[J].中国呼吸与危重监护杂志, 2016, 15(4): 415-417. DOI:10.7507/1671-6205.2016098
[16] 辛丽, 白天, 周剑芳,等. 中国职业暴露人群感染H9N2 禽流感病毒血清学调查[J]. 疾病监测, 2015, 30 (5): 368-370. DOI:10.3784/j.issn.1003-9961.2015.05.007
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