苏云金芽胞杆菌制剂及其与小卷蛾斯氏线虫对腰果重要害虫的作用效果
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摘要
室内测定了苏云金芽胞杆菌(Bacillus thuringiensis,Bt)对腰果重要害虫腰果细蛾(Acrocercops syngramma)和腰果云翅斑螟(Nephopteryx sp.)幼虫的作用效果。研究表明,在500或1000倍浓度下,Bt对低龄(1～3龄)腰果细蛾幼虫的致死作用较强,致死率为70%～100%,但对高龄(4龄和5龄)腰果细蛾幼虫致死作用较低(20.0%～52.5%)。Bt对腰果云翅斑螟3龄幼虫的致死作用相对较慢,在500倍浓度下作用120 h后致死率为75%。2000倍Bt与200 IJs/皿小卷蛾斯氏线虫Steinernema carpocapsae All对腰果云翅斑螟联合作用48 h后表现为增效作用,致死率达90%。
The efficacy of Bacillus thuringiensis(Bt) to control cashew leaf miner(Acrocercops syngramma) larvae and cashew apple and nut borer larvae(Nephopteryx sp.) was tested in the laboratory. The results showed that, at the dose of 500 or 1000 dilution, Bt had a stronger toxicity against the 1st-3rd instar of A. syngramma larvae(70%-100% mortalities) and a lower toxicity against the 4st-5rd instar(20.0%-52.5% mortalities). The lethal efficacy of Bt to the 3rd instar of Nephopteryx sp. was only 75% mortality after 120 h treatment at the dose of 500 dilution. The synergistic interaction was observed, after 48 h treatment in the combination of Bt 2000 dilution and 200 IJs per disc of Steinernema carpocapsae All to the 3rd instar of Nephopteryx sp. larvae.
The efficacy of Bacillus thuringiensis(Bt) to control cashew leaf miner(Acrocercops syngramma) larvae and cashew apple and nut borer larvae(Nephopteryx sp.) was tested in the laboratory. The results showed that, at the dose of 500 or 1000 dilution, Bt had a stronger toxicity against the 1st-3rd instar of A. syngramma larvae(70%-100% mortalities) and a lower toxicity against the 4st-5rd instar(20.0%-52.5% mortalities). The lethal efficacy of Bt to the 3rd instar of Nephopteryx sp. was only 75% mortality after 120 h treatment at the dose of 500 dilution. The synergistic interaction was observed, after 48 h treatment in the combination of Bt 2000 dilution and 200 IJs per disc of Steinernema carpocapsae All to the 3rd instar of Nephopteryx sp. larvae.
引文
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[16]曹春霞,王沫,龙同,等.16000 IU/mg苏云金杆菌悬浮剂对稻纵卷叶螟的防治效果[J].湖北农业科学,2012,51(23):5352-5353,5356.
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[22]Athalye S S,Patil R S.Bionomics,seasonal incidence and chemical control of cashew leaf miner[J].Journal of Maharashtra Agricultural Universities,1998,23:29-31.
[23]韩日畴.昆虫病原线虫大量培养系统的优化管理[D].广州:华南农业大学,1995.
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[27]Koppenh?fer A M,Brown I M,Gaugler R,et al.Synergism of entomopathogenic nematodes and imidacloprid against white grubs:greenhouse and field evaluation[J].Biological Control,2000,19(3):245-251.
[28]张中润,曹莉,刘秀玲,等.昆虫病原线虫Steinernema longicaudum X-7增效药剂的筛选[J].昆虫知识,2006,43(1):68-73.
[29]Oestergaard J,Belau C,Strauch O,et al.Biological control of Tipula paludosa(Diptera:Nematocera)using entomopathogenic nematodes(Steinernema spp.)and Bacillus thuringiensis subsp.israelensis[J].Biological Control,2006,39(3):525-531.
[30]Salem S A,Abdel-Rahman H A,Zebitz C P W,et al.Interaction between entomopathogenic nematodes and Bacillus thuringiensis as a new approach for biological control of some Lepidopterous pests[J].Journal of Applied Sciences Research,2007,3(5):333-342.
[31]张中润,王金辉,黄海杰,等.昆虫病原线虫对腰果云翅斑螟的室内致病力[J].植物保护,2017,43(1):210-213.
[2]梁李宏,张中润.腰果病虫害[M].北京:中国农业出版社,2007:98-100.
[3]梁李宏,张中润.海南腰果病虫害及其防治[J].热带作物学报,2007,28(1):76-79.
[4]张中润,梁李宏,黄伟坚,等.5个不同腰果品系对腰果细蛾抗虫性研究[J].植物保护,2008,34(5):113-115.
[5]张中润,王金辉,黄海杰,等.昆虫病原线虫对腰果细蛾的致病力测定[J].环境昆虫学报,2016,38(3):602-606.
[6]Abraham E V.Pests of cashew(Anacardium occidentale L.)in south India[J].Indian Journal of Agricultural Sciences,1958,28(4):531-544.
[7]罗永明,金启安.腰果云翅斑螟的初步研究[J].热带作物学报,1986,7(2):99-105.
[8]潘贤丽,邢福易.腰果蛀果斑螟的发生与防治[J].热带作物学报,1987,8(1):109-116.
[9]Sanahuja G,Banakar R,Twyman R M,et al.Bacillus thuringiensis:a century of research,development and commercial applications[J].Plant Biotechnology Journal,2011,9(3):283-300.
[10]Sanchis V.From microbial sprays to insect-resistant transgenic plants:history of the biopesticide Bacillus thuringiensis.A review[J].Agronomy for Sustainable Development,2011,31(1):217-231.
[11]Schnepf E,Crickmore N,Van Rie J,et al.Bacillus thuringiensis and its pesticidal crystal proteins[J].Microbiology and Molecular Biology Reviews,1998,62(3):775-806.
[12]Bravo A,Likitvivatanavong S,Gill S S,et al.Bacillus thuringiensis:A story of a successful bioinsecticide[J].Insect Biochemistry&Molecular Biology,2011,41(7):423-431.
[13]李燕利,胡春祥,高佳.Bt对分月扇舟蛾体毒性及体内保护酶系影响[J].东北林业大学学报,2011,39(7):93-95.
[14]Han G,Li C,Liu Q,et al.Synergistic effect of combining Plutella xylostella Granulovirus and Bacillus thuringiensis at sublethal dosages on controlling of diamondback moth(Lepidoptera:Plutellidae)[J].Journal of Economic Entomology,2015,108(5):2184-2191.
[15]江俊起,王向阳,董猛,等.Bt制剂防治玉米螟的田间效果[J].植物保护,2011,37(4):173-175.
[16]曹春霞,王沫,龙同,等.16000 IU/mg苏云金杆菌悬浮剂对稻纵卷叶螟的防治效果[J].湖北农业科学,2012,51(23):5352-5353,5356.
[17]Stapel J O,Waters D J,Ruberson J R,et al.Development and behavior of Spodoptera exigua(Lepidoptera:Noctuidae)larvae in choice tests with food substrates containing toxins of Bacillus thuringiensis[J].Biological Control,1998,11(1):29-37.
[18]张中润,王金辉,黄伟坚,等.腰果云翅斑螟幼虫发生动态及空间分布型研究[J].热带作物学报,2011,32(6):1153-1157.
[19]张中润,王金辉,黄伟坚,等.海南腰果云翅斑螟发生为害特点[J].植物保护,2012,38(5):158-161.
[20]喻子牛.微生物农药及其产业化[M].北京:科学出版社,2000.
[21]张宏宇,喻子牛.影响苏云金芽孢杆菌制剂防虫效果的因素[J].植物保护,1997,23(4):41-44.
[22]Athalye S S,Patil R S.Bionomics,seasonal incidence and chemical control of cashew leaf miner[J].Journal of Maharashtra Agricultural Universities,1998,23:29-31.
[23]韩日畴.昆虫病原线虫大量培养系统的优化管理[D].广州:华南农业大学,1995.
[24]Woodring J L,Kaya H K.Steinernematid and Heterorhabditid nematodes:A handbook of biology and techniques[M].Arkansas Agricultural Experiment Station,1988.
[25]Koppenh?fer A M,Cowles R S,Cowles E A,et al.Comparison of neonicotinoid insecticides as synergists for entomopathogenic nematodes[J].Biological Control,2002,24(1):90-97.
[26]Koppenh?fer A M,Kaya H K.Synergism of imidacloprid and an entomopathogenic nematode:a novel approach to white grub(Coleoptera:Scarabaeidae)control in turfgrass[J].Journal of Economic Entomology,1998,91(3):618-623.
[27]Koppenh?fer A M,Brown I M,Gaugler R,et al.Synergism of entomopathogenic nematodes and imidacloprid against white grubs:greenhouse and field evaluation[J].Biological Control,2000,19(3):245-251.
[28]张中润,曹莉,刘秀玲,等.昆虫病原线虫Steinernema longicaudum X-7增效药剂的筛选[J].昆虫知识,2006,43(1):68-73.
[29]Oestergaard J,Belau C,Strauch O,et al.Biological control of Tipula paludosa(Diptera:Nematocera)using entomopathogenic nematodes(Steinernema spp.)and Bacillus thuringiensis subsp.israelensis[J].Biological Control,2006,39(3):525-531.
[30]Salem S A,Abdel-Rahman H A,Zebitz C P W,et al.Interaction between entomopathogenic nematodes and Bacillus thuringiensis as a new approach for biological control of some Lepidopterous pests[J].Journal of Applied Sciences Research,2007,3(5):333-342.
[31]张中润,王金辉,黄海杰,等.昆虫病原线虫对腰果云翅斑螟的室内致病力[J].植物保护,2017,43(1):210-213.
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