绿原酸长循环脂质体中绿原酸含量测定方法的建立

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英文篇名：Establishment of the Content Determination Method for Chlorogenic Acid in PEGylated Liposomes 作者：刘东东 ; 孟盈盈 ; 叶军 ; 刘玉玲 ; 杨艳芳 英文作者：Liu Dongdong;Meng Yingying;Ye Jun;Liu Yuling;Yang Yanfang;Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College;Beijing Wehand-Bio Pharmaceutical Co.Ltd.; 关键词：绿原酸 ; 绿原酸长循环脂质体 ; 高效液相色谱法 ; 含量测定 英文关键词：Chlorogenic acid;;PEGylated liposomes containing chlorogenic acid;;HPLC;;Determination of content 中文刊名：ZYSG 英文刊名：China Pharmacist 机构：中国医学科学院北京协和医学院药物研究所;北京五和博澳药业有限公司; 出版日期：2019-03-05 出版单位：中国药师 年：2019 期：v.22 基金：重大新药创制科技重大专项(编号:2018ZX09711001-002-005、2018ZX09721003-009-001);; 北京市自然科学基金项目(编号:7162135);; 协和青年基金项目(编号:3332015139);; 中国博士后基金资助项目(编号:2017M620679) 语种：中文; 页：ZYSG201903011 页数：5 CN：03 ISSN：42-1626/R 分类号：45-49

摘要

目的:筛选合适的溶剂以消除绿原酸的溶剂化效应并满足脂质体中绿原酸含量测定要求,建立高效液相色谱法(HPLC)用于绿原酸长循环脂质体中的绿原酸含量测定。方法:HPLC色谱条件以Agilent SB-C_(18)(250 mm×4.6 mm,5μm)作为色谱柱,乙腈-0.4%磷酸(18∶82)作为流动相,检测波长328 nm,流速1 ml·min~(-1),进样量10μl,柱温25℃。分别以水和不同体积分数的无水乙醇为溶剂溶解绿原酸进行HPLC谱图分析,采用优选的溶剂对绿原酸长循环脂质体进行含量测定及方法学研究。结果:绿原酸原料药在无水乙醇中会由于溶剂化效应而产生双峰,而在30%的乙醇中无溶剂化效应且对绿原酸长循环脂质体的溶解性良好。因此,选择30%的乙醇作为含量测定用溶剂。在20.1～120.6μg·ml~(-1)范围内,绿原酸浓度与峰面积呈良好的线性关系,r=0.999 9。绿原酸长循环脂质体含量测定的平均回收率为101.38%(RSD=0.96%,n=9)。结论:本方法简单、快速、灵敏度高、重复性好,可以作为绿原酸长循环脂质体中绿原酸含量测定的方法。
        Objective: To select suitable solvents to eliminate the solvating effect,and establish an HPLC method for the determination of chlorogenic acid (CA) in PEGylated liposomes. Methods: Water and ethanol with different volume partitions were used to dissolve CA in order to screen the suitable solvent. HPLC was used to study the content of CA containing in the liposomes. The mobile phase was composed of acetonitrile and 0.4% phosphoric acid (18 ∶82) at the flow rate of 1 ml·min~(-1) and the detection wavelength was 328 nm. An Agilent SB-C C_(18) (250 mm×4.6 mm,5 μm) analytical column was used with the column temperature of 25℃. The injection volume was 10 μl. Results: CA could be dissolved in anhydrous ethanol while couldn't be dissolved in 30% ethanol,therefore,30% ethanol was selected as the solvent for CA determination. The HPLC results showed that the concentration of CA and the peak area showed a good linear relationship within the range of 20.1-120.6 μg · ml~(-1). The average recovery was 101.38% and the RSD was0.96% (n = 9). Conclusion: The method for CA determination established in this study is simple,rapid,sensitive and reproducible,which can be used as a method for CA determination in PEGylated liposomes.

引文

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