番茄种质资源主要植物学性状的遗传多样性及相关性
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摘要
为拓宽番茄品种选育材料的遗传基础,以西北农林科技大学园艺学院番茄种质资源库中的142份番茄种质为研究材料,对植株、产量、果实和品质等方面的47个性状进行遗传多样性和相关性分析,研究番茄种质的遗传多样性及各性状间的相关性。结果表明:47个性状的遗传多样性指数平均为2.08,其中27个质量性状的遗传多样性指数平均为0.65,果型的遗传多样性指数最高(1.44),株型遗传多样性指数最低(0.04);20个数量性状的遗传多样性指数平均为4.01,心室数的遗传多样性指数最小(2.41),果横径的遗传多样性指数最高(4.79)。数量性状的变异系数平均为45.94%,除叶片长之外,其余各性状变异系数均高于平均值。数量性状中单花序花数和单花序果数属于强变异,其余均为中等变异。142份番茄种质为自然群体,遗传变异较丰富,可用于开展番茄重要目标性状的关联分析作图。
27 qualitative and 20 quantitative traits of 142 tomato germplasm resources from tomato germplasm resources bank in College of Horticulture,Northwest A&F University were studied by analyzing the genetic diversity and correlations between different traits to broaden the genetic basis of breeding materials in tomato breeding.Result:The average value of genetic diversity index of total 47 traits and 27 qualitative traits is 2.08 and 0.65 respectively.The genetic diversity index of fruit shape and plant type is 1.44(the highest)and 0.04(the lowest)separately.The average value of genetic diversity index of 20 quantitative traits is 4.01.The genetic diversity index of fruit transverse diameter and locule number is 4.79(the highest)and 2.41(the lowest)respectively.The average value of variation coefficients of quantitative traits is 45.94%and the variation coefficients of different quantitative traits all are higher than the average value except for leaf length.The flower number per inflorescence and fruit number per inflorescence in 20 quantitative traits belong to strong variation and other traits belong to intermediate variation.142 tomato germplasm resources with abundance genetic variation can be used for correlation analysis of important target traits.
27 qualitative and 20 quantitative traits of 142 tomato germplasm resources from tomato germplasm resources bank in College of Horticulture,Northwest A&F University were studied by analyzing the genetic diversity and correlations between different traits to broaden the genetic basis of breeding materials in tomato breeding.Result:The average value of genetic diversity index of total 47 traits and 27 qualitative traits is 2.08 and 0.65 respectively.The genetic diversity index of fruit shape and plant type is 1.44(the highest)and 0.04(the lowest)separately.The average value of genetic diversity index of 20 quantitative traits is 4.01.The genetic diversity index of fruit transverse diameter and locule number is 4.79(the highest)and 2.41(the lowest)respectively.The average value of variation coefficients of quantitative traits is 45.94%and the variation coefficients of different quantitative traits all are higher than the average value except for leaf length.The flower number per inflorescence and fruit number per inflorescence in 20 quantitative traits belong to strong variation and other traits belong to intermediate variation.142 tomato germplasm resources with abundance genetic variation can be used for correlation analysis of important target traits.
引文
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[24]LORSON M A,HORVOTZ H R,VAN DEN HEU-VEL S.lin-5is a novel component of the spindle apparatus required for chromosome segregation and cleavage plane specification in Caenorhabditis elegans[J].The Journal of cell biology,2000,148:73-86.
[25]LIPPMAN Z,TANKSLEY S D.Dissecting the genetic pathway to extreme fruit size in tomato using a cross between the small-fruited wild species Lycopersicon pimpinellifolium and L.esculentum var.Giant Heirloom[J].Genetics,2001,158:413-422.
[26]YAN J,SHAH T,WARBURTON M L,et al.Genetic characterization and linkage disequilibrium estimation of a global maize collection using SNP markers[J].PLoS ONE,2009,4:e8451.
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[28]THORNSBERRY J M,GOODMAN M M,DOEB-LEY J,et al.Dwarf8polymorphisms associate with variation in flowering time[J].Nature genetics,2001,28(3):286-289.
[29]郝转芳,苏治军,李亮,等.基于SNP标记的关联分析在玉米耐旱研究中的应用[J].作物杂志,2009,133(06):1-7.Hao ZF,Su ZJ,li L,Zhang SH,Li XH.Based on correlation analysis of SNP marker application in drought-tolerant corn research[J].Crops,2009,133(6):1-7.(in Chinese)
[30]WILSON L M,WHITT S R,IB EZ A M,et al.Dissection of maize kernel composition and starch production by candidate gene association[J].The Plant Cell,2004,16(10):2719-2733.
[31]ALURU M,XU Y,GUO R,et al.Generation of transgenic maize with enhanced provitamin A content[J].Journal of Experimental Botany,2008,59(13):3551-3562.
[32]BRESEGHELLO F,SORRELLS M E.Association mapping of kernel size and milling quality in wheat(Triticum aestivum L.)cultivars[J].Genetics,2006,172(2):1165-1177.
[33]GOUDEMAND E,LAURENT V,DUCHALSIS L,et al.Association mapping and meta-analysis:two complementary approaches for the detection of reliable Septoria tritici blotch quantitative resistance in bread wheat(Triticum aestivum L.)[J].Molecular Breeding,2013,32(3):563-584.
[34]OLSEN O A.Nuclear endosperm development in cereals and Arabidopsis thaliana[J].The Plant Cell,2004,16(suppl 1):S214-S227.
[35]ARANZANA M J,KIM S,ZHAO K,et al.Genomewide association mapping in Arabidopsis identifies previously known flowering time and pathogen resistance genes[J].PLoS Genetics,2005,1(5):e60.
[36]孙中永,程爽,王继变,等.油菜含油量性状QTL关联分析及有利等位基因在品种中的构成分析[J].中国农业科学,2012,45(19):3921-3931.
[37]危文亮,张艳欣,吕海霞,等.芝麻资源群体结构及含油量关联分析[J].中国农业科学,2012,45(10):1895-903.
[38]张艳欣,王林海,黎冬华,等.芝麻茎点枯病抗性关联分析及抗病载体材料挖掘[J].中国农业科学,2012,45(13):2580-2591.
[39]赵波,叶剑,金文林,等.不同类型小豆种质SSR标记遗传多样性及性状关联分析[J].中国农业科学,2011,44(4):673-682.
[40]王海平,李锡香,程嘉琪,等.中国大蒜种质资源遗传多样性和群体遗传结构分析[J].中国农业科学,2012,45(16):3318-3329.
[41]范虎,文自翔,王春娥,等.中国野生大豆群体农艺加工性状与SSR关联分析和特异材料的遗传构成[J].作物学报,2013,775-788.
[42]张军,赵团结,盖钧镒.大豆育成品种农艺性状QTL与SSR标记的关联分析[J].作物学报,2008,34(12):2059-2069.
[43]SUN Y,LIANG Y,WU J,et al.Dynamic QTL analysis for fruit lycopene content and total soluble solid content in a Solanum lycopersicum x S.pimpinellifoliumcross[J].Genetics and Molecular Research,2012,11(4):3696-3710.
[44]徐明磊.番茄高可溶性固形物种质的创造及相关基因的差异表达研究[D].重庆:西南大学,2006.
[45]VERLAAN M G,SZINAY D,HUTTON S F,et al.Chromosomal rearrangements between tomato and Solanum chilense hamper mapping and breeding of the TYLCV resistance gene Ty-1[J].Plant Journal,2011,68(6):1093-1103.
[46]ALPERT K,GRANDILO S,TANKSLEY S.a major QTL controlling fruit weight is common to both redand green-fruited tomato species[J].Theoretical and Applied Genetics,1995,91:994-1000.
[47]AZANZA F,YOUNG T,KIM D,et al.Characterization of the effect of introgressed segments of chromosome 7and 10fromLycopersion chmielewskii on tomato soluble solids,pH,and yield[J].Theoretical and Applied Genetics,1994,87(8):965-972.
[48]ESHED Y,ZAMIR D.A genomic library of Lycopersicon pennellii in L.esculentum:a tool for fine mapping of genes[J].Euphytica,1994,79(3):175-179.
[49]CENTENO D C,OSORIO S,NUNES-NESI A,et al.Malate plays a crucial role in starch metabolism,ripening,and soluble solid content of tomato fruit and affects postharvest softening[J].The Plant Cell,2011,23(1):162-184.
[50]LUENGWILAI K,FIEHN O E,BECKLES D M.Comparison of leaf and fruit metabolism in two tomato(Solanum lycopersicum L.)genotypes varying in total soluble solids[J].Journal of Agricultural and Food Chemistry,2010,58(22):11790-11800.
[2]KLEE H J,TIEMAN D M.Genetic challenges of flavor improvement in tomato[J].Trends in Genetics,2013,29:257-262.
[3]孙亚东,梁燕,吴江敏,等.番茄种质资源的遗传多样性和聚类分析[J].西北农业学报,2009,18:297-301.
[4]李锡香,杜永臣.番茄种质资源描述规范和数据标准[M].中国农业出版社,2006.
[5]尚建立,王吉明,郭琳琳,等.西瓜种质资源主要植物学性状的遗传多样性及相关性分析[J].植物遗传资源学报,2012(13):11-15.
[6]SZAMOSI C,SOLMAZLI,SARI N,et al.Morphological characterization of Hungarian and Turkish watermelon(Citrullus lanatus(Thunb.)Matsum.et Nakai)genetic resources[J].Genetic Resources and Crop Evolution,2009,56:1091-1105.
[7]RANC N,MU OS S,SANTONI S,et al.A clarified position for Solanum lycopersicumvar.cerasiforme in the evolutionary history of tomatoes(Solanaceae)[J].BMC plant biology,2008,8:130.
[8]CAUSSE M,BURET M,ROBINI K,et al.Inheritance of nutritional and sensory quality traits in fresh market tomato and relation to consumer preferences[J].Journal of food science,2003,68:2342-2350.
[9]DAVIES J N,HOBSON G E,MCGLASSON W.The constituents of tomato fruit-the influence of environment,nutrition,and genotype[J].Critical Reviews in Food Science&Nutrition,1981,15:205-280.
[10]XU J X,RANC N,MUNOS S,et al.Phenotypic diversity and association mapping for fruit quality traits in cultivated tomato and related species[J].Theoretical and Applied Genetics,2013,126:567-581.
[11]PATERSON A H,DAMON S,HEWITT J,et al.Mendelian factors underlying quantitative traits in tomato:comparison across species,generations,and environments[J].Genetics,1991,127:181-197.
[12]SALIBA-COLOMBANI V,CAUSSE M,Langlois D,et al.Genetic analysis of organoleptic quality in fresh market tomato.1.Mapping QTLs for physical and chemical traits[J].Theoretical and Applied Genetics,2001,102:259-272.
[13]WANG J,WAN X,CROSSA J,et al.QTL mapping of grain length in rice(Oryza sativa L.)using chromosome segment substitution lines[J].Genetical research,2006,88:93-104.
[14]SZALMA S,HOSTERTt B,LEDEAUX J,et al.QTLmapping with near-isogenic lines in maize[J].Theoretical and Applied Genetics,2007,114:1211-1228.
[15]ORSINI E,KRCHOV L,UPHAUS J,et al.Mapping of QTL for resistance to first and second generation of European corn borer using an integrated SNP and SSR linkage map[J].Euphytica,2012,183:197-206.
[16]ESHED Y,ZAMIR D.An introgression line population of Lycopersicon pennellii in the cultivated tomato enables the identification and fine mapping of yieldassociated QTL[J].Genetics,1995,141:1147.
[17]GOLDMAN I,PARAN I,ZAMIR D.Quantitative trait locus analysis of a recombinant inbred line population derived from a Lycopersicon esculentum x Lycopersicon cheesmanii cross[J].Theoretical and Applied Genetics,1995,90:925-932.
[18]REITER L T,MURAKAMI T,KOEUTH T,et al.Arecombination hotspot responsible for two inherited peripheral neuropathies is located near a mariner transposon-like element[J].Nature genetics,1996,12:288-297.
[19]VAN DER KNAAP E,TANKSLEY S.The making of a bell pepper-shaped tomato fruit:identification of loci controlling fruit morphology in Yellow Stuffer tomato[J].Theoretical and Applied Genetics,2003,107:139-147.
[20]CAUSSE M,DUFFE P,GOMEZ M,et al.A genetic map of candidate genes and QTLs involved in tomato fruit size and composition[J].Journal of Experimental Botany,2004,55:1671-1685.
[21]LECOMTE L,SALIBA-COLOMBANI V,GAUTIERA,et al.Fine mapping of QTLs of chromosome 2affecting the fruit architecture and composition of tomato[J].Molecular breeding,2004,13:1-14.
[22]VAN DER KNAAP E,TANKSLEY S.Identification and characterization of a novel locus controlling early fruit development in tomato[J].Theor etical and Applied Genetics,2001,103:353-358.
[23]FRARY A,NESBITT T C,FRARY A,et al.a quantitative trait locus key to the evolution of tomato fruit size[J].Science,2000,289:85-88.
[24]LORSON M A,HORVOTZ H R,VAN DEN HEU-VEL S.lin-5is a novel component of the spindle apparatus required for chromosome segregation and cleavage plane specification in Caenorhabditis elegans[J].The Journal of cell biology,2000,148:73-86.
[25]LIPPMAN Z,TANKSLEY S D.Dissecting the genetic pathway to extreme fruit size in tomato using a cross between the small-fruited wild species Lycopersicon pimpinellifolium and L.esculentum var.Giant Heirloom[J].Genetics,2001,158:413-422.
[26]YAN J,SHAH T,WARBURTON M L,et al.Genetic characterization and linkage disequilibrium estimation of a global maize collection using SNP markers[J].PLoS ONE,2009,4:e8451.
[27]孙建昌,曹桂兰,李亚非,等.水稻地方品种群体内的遗传多样性分析[J].西北农林科技大学学报:自然科学版,2011,39(12):145-52.(in Chinese)
[28]THORNSBERRY J M,GOODMAN M M,DOEB-LEY J,et al.Dwarf8polymorphisms associate with variation in flowering time[J].Nature genetics,2001,28(3):286-289.
[29]郝转芳,苏治军,李亮,等.基于SNP标记的关联分析在玉米耐旱研究中的应用[J].作物杂志,2009,133(06):1-7.Hao ZF,Su ZJ,li L,Zhang SH,Li XH.Based on correlation analysis of SNP marker application in drought-tolerant corn research[J].Crops,2009,133(6):1-7.(in Chinese)
[30]WILSON L M,WHITT S R,IB EZ A M,et al.Dissection of maize kernel composition and starch production by candidate gene association[J].The Plant Cell,2004,16(10):2719-2733.
[31]ALURU M,XU Y,GUO R,et al.Generation of transgenic maize with enhanced provitamin A content[J].Journal of Experimental Botany,2008,59(13):3551-3562.
[32]BRESEGHELLO F,SORRELLS M E.Association mapping of kernel size and milling quality in wheat(Triticum aestivum L.)cultivars[J].Genetics,2006,172(2):1165-1177.
[33]GOUDEMAND E,LAURENT V,DUCHALSIS L,et al.Association mapping and meta-analysis:two complementary approaches for the detection of reliable Septoria tritici blotch quantitative resistance in bread wheat(Triticum aestivum L.)[J].Molecular Breeding,2013,32(3):563-584.
[34]OLSEN O A.Nuclear endosperm development in cereals and Arabidopsis thaliana[J].The Plant Cell,2004,16(suppl 1):S214-S227.
[35]ARANZANA M J,KIM S,ZHAO K,et al.Genomewide association mapping in Arabidopsis identifies previously known flowering time and pathogen resistance genes[J].PLoS Genetics,2005,1(5):e60.
[36]孙中永,程爽,王继变,等.油菜含油量性状QTL关联分析及有利等位基因在品种中的构成分析[J].中国农业科学,2012,45(19):3921-3931.
[37]危文亮,张艳欣,吕海霞,等.芝麻资源群体结构及含油量关联分析[J].中国农业科学,2012,45(10):1895-903.
[38]张艳欣,王林海,黎冬华,等.芝麻茎点枯病抗性关联分析及抗病载体材料挖掘[J].中国农业科学,2012,45(13):2580-2591.
[39]赵波,叶剑,金文林,等.不同类型小豆种质SSR标记遗传多样性及性状关联分析[J].中国农业科学,2011,44(4):673-682.
[40]王海平,李锡香,程嘉琪,等.中国大蒜种质资源遗传多样性和群体遗传结构分析[J].中国农业科学,2012,45(16):3318-3329.
[41]范虎,文自翔,王春娥,等.中国野生大豆群体农艺加工性状与SSR关联分析和特异材料的遗传构成[J].作物学报,2013,775-788.
[42]张军,赵团结,盖钧镒.大豆育成品种农艺性状QTL与SSR标记的关联分析[J].作物学报,2008,34(12):2059-2069.
[43]SUN Y,LIANG Y,WU J,et al.Dynamic QTL analysis for fruit lycopene content and total soluble solid content in a Solanum lycopersicum x S.pimpinellifoliumcross[J].Genetics and Molecular Research,2012,11(4):3696-3710.
[44]徐明磊.番茄高可溶性固形物种质的创造及相关基因的差异表达研究[D].重庆:西南大学,2006.
[45]VERLAAN M G,SZINAY D,HUTTON S F,et al.Chromosomal rearrangements between tomato and Solanum chilense hamper mapping and breeding of the TYLCV resistance gene Ty-1[J].Plant Journal,2011,68(6):1093-1103.
[46]ALPERT K,GRANDILO S,TANKSLEY S.a major QTL controlling fruit weight is common to both redand green-fruited tomato species[J].Theoretical and Applied Genetics,1995,91:994-1000.
[47]AZANZA F,YOUNG T,KIM D,et al.Characterization of the effect of introgressed segments of chromosome 7and 10fromLycopersion chmielewskii on tomato soluble solids,pH,and yield[J].Theoretical and Applied Genetics,1994,87(8):965-972.
[48]ESHED Y,ZAMIR D.A genomic library of Lycopersicon pennellii in L.esculentum:a tool for fine mapping of genes[J].Euphytica,1994,79(3):175-179.
[49]CENTENO D C,OSORIO S,NUNES-NESI A,et al.Malate plays a crucial role in starch metabolism,ripening,and soluble solid content of tomato fruit and affects postharvest softening[J].The Plant Cell,2011,23(1):162-184.
[50]LUENGWILAI K,FIEHN O E,BECKLES D M.Comparison of leaf and fruit metabolism in two tomato(Solanum lycopersicum L.)genotypes varying in total soluble solids[J].Journal of Agricultural and Food Chemistry,2010,58(22):11790-11800.
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