中药及其有效成分逆转耐药性机制研究探析
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摘要
目的:系统了解中药逆转耐药性机制的研究进展及现状,以便更好指导临床前新药研发。方法:本文通过采集整合国内外相关文献,对中药及其有效成分的逆转耐药性的作用机制方面作一综述。结果:目前对中药逆转耐药性的研究主要从整体动物模型、体外实验、细胞模型、蛋白基因等多层次进行,并证明中药逆转耐药性主要通过对机体免疫系统以及相关蛋白、基因的影响而发挥作用。结论:选用合适的研究方法可以揭示中药逆转耐药性机制,研究开发具有逆转耐药性的中药,为中药在逆转耐药性的应用提供理论依据。
Objective: To understand the research progress systematically and status of the mechanism of reverses resistance of traditional Chinese medicine, and better guide the research and development of new drug. Methods: This paper reviewed the mechanism of reverse resistance of traditional Chinese medicine and its effective components by collecting and integrating relevant literatures. Results: At present, the study on the reverse resistance of traditional Chinese medicine was mainly from the whole animal models and in vitro experiments, cell, protein gene and other multilevel model, and proved that Chinese medicine reverse resistance was mainly based on the body’s immune system and the influence of related proteins, genes and played a role. Conclusion: The appropriate research method can be used to reveal the mechanism of reverse resistance of traditional Chinese medicine, and research and development of traditional Chinese medicine that can reverse drug resistance, and provide theoretical basis for TCM to reverse the application of drug resistance.
Objective: To understand the research progress systematically and status of the mechanism of reverses resistance of traditional Chinese medicine, and better guide the research and development of new drug. Methods: This paper reviewed the mechanism of reverse resistance of traditional Chinese medicine and its effective components by collecting and integrating relevant literatures. Results: At present, the study on the reverse resistance of traditional Chinese medicine was mainly from the whole animal models and in vitro experiments, cell, protein gene and other multilevel model, and proved that Chinese medicine reverse resistance was mainly based on the body’s immune system and the influence of related proteins, genes and played a role. Conclusion: The appropriate research method can be used to reveal the mechanism of reverse resistance of traditional Chinese medicine, and research and development of traditional Chinese medicine that can reverse drug resistance, and provide theoretical basis for TCM to reverse the application of drug resistance.
引文
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2顾海彤,鲁辛辛.微生物感染实验室与临床沟通案例解析.中华临床实验室管理电子杂志,2018,6(1):52-57.
3崔煦然,赵京霞,郭玉红,等.细菌耐药背景下中药抗菌作用研究进展.世界中医药,2016,11(10):1940-1944.
4温红侠,陈一强,朱莲娜,等.绿原酸对铜绿假单胞菌生物膜干预作用的体外研究.中华医院感染学杂志,2009,19(12):1478-1481.
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6李雪.苦参碱对表皮葡萄球菌密度感应系统AI-2信号分子的调控作用研究.银川:宁夏大学硕士研究生学位论文,2016.
7于军,于红,肖洋,等.射干提取液对铜绿假单胞菌PA11株R质粒体内外消除作用.吉林大学学报(医学版),2003,29(2):170-171.
8张文平,曹镐禄,张文书,等.千里光对大肠埃希菌R质粒消除作用的血清药理学研究.广东医学,2007,28(8):1238.
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11贾云鹏,程宁.中药对大肠埃希菌抗生素耐药性逆转作用的实验研究.陕西中医,2009,30(3):366.
12陈勇川,谢林利,熊丽蓉,等.黄芩苷/黄芩素对耐甲氧西林金黄色葡萄球菌抗药性的逆转作用研究.中国药房,2008,19(9):644.
13李娟,李小宁,钟正灵,等.中药单体白花丹醌对鲍曼不动杆菌的耐药逆转作用.中国临床药理学与治疗学,2015,20(2):155-159.
14游思湘.中药抑制剂血清药理学研究及对相关基因影响.长沙:湖南农业大学硕士研究生学位论文,2011.
15邢茜,江荣林,马伟斌,等.甲氧西林金黄色葡萄球菌的研究进展.中华医院感染学杂志,2014,24(14):3638-3640.
16万艳红,葛银林,李斌,等.耐甲氧西林金黄色葡萄球菌耐药机制和临床耐药研究的新进展.中国药物与临床,2013,13(9):1164-1167.
17张驰,贾旭,杨羚,等.中药单体白花丹醌对替加环素耐药鲍曼不动杆菌的抗菌作用研究.成都医学院学报,2017,12(2):117-121.
18 Coburn P S,Pillar C M,Jett B D,et al.Enterococcus faecalis senses target cells and in response expresses cytolysin.Science,2004,306(5705):2270-2272.
19 Coyne S,Courvalin P,Périchon B.Efflux-mediated antibiotic resistance in Acinetobacter spp.Antimicrob Agents Che-mother,2011,55(3):947-953.
20 Choe H S,Son S W,Choi H A,et al.Analysis of the distribution of bacteria within urinary catheter biofilms using four different molecular techniques.Am J Infect Control,2012,40(9):249-254.
21宋战昀,冯新,韩文瑜,等.金黄色葡萄球菌norA外输泵中药耐药抑制剂的筛选.吉林农业大学学报,2007,29(3):329.
22管翠萍,冯磊,乔霞,等.不同中药提取物对耐甲氧西林金黄色葡萄球菌的抑菌作用和逆转机制.中国兽医学报,2015,35(12):1997-2001.
23 Sun J R,Chan M C,Chang T Y,et al.Overexpression of the adeb gene in clinical isolates of tigecycline-nonsuseceptible Acinetobacter baumannii without insertion mutations in ade SR.Antimicrob Agents Chemother,2010,54(11):4934-4938.
24郭威,周莹,叶露,等.穿心莲内酯抑制铜绿假单胞菌外排泵MexAB-OprM的作用.中国医院药学杂志,2010,30(16):1343.
25 George T,Frank R.Mutidrug pump inhibitors uncover remarkable activity of plant antimicrobials.Antimicrobial Agent and Chemotherapy,2002,46(10):3133.
2顾海彤,鲁辛辛.微生物感染实验室与临床沟通案例解析.中华临床实验室管理电子杂志,2018,6(1):52-57.
3崔煦然,赵京霞,郭玉红,等.细菌耐药背景下中药抗菌作用研究进展.世界中医药,2016,11(10):1940-1944.
4温红侠,陈一强,朱莲娜,等.绿原酸对铜绿假单胞菌生物膜干预作用的体外研究.中华医院感染学杂志,2009,19(12):1478-1481.
5宋鸿,金瑛,王明胜.苦参对铜绿假单胞菌生物膜干预作用的体外研究.遵义医学院学报,2015,38(3):235-238.
6李雪.苦参碱对表皮葡萄球菌密度感应系统AI-2信号分子的调控作用研究.银川:宁夏大学硕士研究生学位论文,2016.
7于军,于红,肖洋,等.射干提取液对铜绿假单胞菌PA11株R质粒体内外消除作用.吉林大学学报(医学版),2003,29(2):170-171.
8张文平,曹镐禄,张文书,等.千里光对大肠埃希菌R质粒消除作用的血清药理学研究.广东医学,2007,28(8):1238.
9 Lauretti L,Riccio M L,Mazzariol A,et al.Cloning and characterizationof bla VIM,a new integron-borne metallo-β-lactamase gene from a Pseudomonas aeruginosa clinical isolate.Antimicrob Agents Chemother,1999,43(7):1584-1590.
10刘平,叶惠芬,陈惠玲,等.5种中药对产酶菌的抑菌作用.中国微生态学杂志,2006,18(1):39.
11贾云鹏,程宁.中药对大肠埃希菌抗生素耐药性逆转作用的实验研究.陕西中医,2009,30(3):366.
12陈勇川,谢林利,熊丽蓉,等.黄芩苷/黄芩素对耐甲氧西林金黄色葡萄球菌抗药性的逆转作用研究.中国药房,2008,19(9):644.
13李娟,李小宁,钟正灵,等.中药单体白花丹醌对鲍曼不动杆菌的耐药逆转作用.中国临床药理学与治疗学,2015,20(2):155-159.
14游思湘.中药抑制剂血清药理学研究及对相关基因影响.长沙:湖南农业大学硕士研究生学位论文,2011.
15邢茜,江荣林,马伟斌,等.甲氧西林金黄色葡萄球菌的研究进展.中华医院感染学杂志,2014,24(14):3638-3640.
16万艳红,葛银林,李斌,等.耐甲氧西林金黄色葡萄球菌耐药机制和临床耐药研究的新进展.中国药物与临床,2013,13(9):1164-1167.
17张驰,贾旭,杨羚,等.中药单体白花丹醌对替加环素耐药鲍曼不动杆菌的抗菌作用研究.成都医学院学报,2017,12(2):117-121.
18 Coburn P S,Pillar C M,Jett B D,et al.Enterococcus faecalis senses target cells and in response expresses cytolysin.Science,2004,306(5705):2270-2272.
19 Coyne S,Courvalin P,Périchon B.Efflux-mediated antibiotic resistance in Acinetobacter spp.Antimicrob Agents Che-mother,2011,55(3):947-953.
20 Choe H S,Son S W,Choi H A,et al.Analysis of the distribution of bacteria within urinary catheter biofilms using four different molecular techniques.Am J Infect Control,2012,40(9):249-254.
21宋战昀,冯新,韩文瑜,等.金黄色葡萄球菌norA外输泵中药耐药抑制剂的筛选.吉林农业大学学报,2007,29(3):329.
22管翠萍,冯磊,乔霞,等.不同中药提取物对耐甲氧西林金黄色葡萄球菌的抑菌作用和逆转机制.中国兽医学报,2015,35(12):1997-2001.
23 Sun J R,Chan M C,Chang T Y,et al.Overexpression of the adeb gene in clinical isolates of tigecycline-nonsuseceptible Acinetobacter baumannii without insertion mutations in ade SR.Antimicrob Agents Chemother,2010,54(11):4934-4938.
24郭威,周莹,叶露,等.穿心莲内酯抑制铜绿假单胞菌外排泵MexAB-OprM的作用.中国医院药学杂志,2010,30(16):1343.
25 George T,Frank R.Mutidrug pump inhibitors uncover remarkable activity of plant antimicrobials.Antimicrobial Agent and Chemotherapy,2002,46(10):3133.
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