环境因子对新疆鹅喉羚群体遗传多样性的影响
|
摘要
环境因素在物种进化和遗传变异过程中起着非常重要的作用。为探讨新疆鹅喉羚遗传多样性与环境因子的关系,本研究采用聚合酶链式反应(PCR)和直接测序的方法,测定了新疆鹅喉羚11个群体84份样本的线粒体DNA Cyt b基因(1 140 bp)和D-loop区(1 100 bp)序列,分析各群体遗传多样性及环境因子对遗传多样性的影响。结果显示新疆鹅喉羚具有较高的单倍型多样性,较低的核苷酸多样性,表明其遗传多样性处于较低水平。环境因子与群体遗传多样性的相关性分析结果表明,海拔、年均降水量、年均气温、人口数量是影响新疆鹅喉羚遗传多样性的主要环境因子,其中海拔是最关键的环境因子。本研究结论为新疆鹅喉羚群体有效合理的保护与管理提供理论依据。
Environmental factors play an important role in the evolution and genetic variation of species.To clarify the relationship between genetic diversity and environmental factors of Gazella subgutturosa in Xinjiang, in this study the mitochondrial DNA cytochrome b gene(1 140 bp) and control region(1 100 bp) sequences of Gazella subgutturosa were analysed from 84 individuals in 11 populations from across the species' natural range within Xinjiang by using PCR and direct sequencing methods.The results showed that the Gazella subgutturosa population in Xinjiang seems to show high haplotype but low nucleotide diversity, these results indicated that the Gazella subgutturosa investigated in this study exhibited low levels of genetic diversity.The results revealed by the analysis of correlation between environmental factors and the genetic diversity of various groups showed that altitude, annual precipitation, annual temperature, population are the main environmental factors affecting the genetic diversity of Gazella subgutturosa in Xinjiang,among which altitude is the key environmental factor.Our results provide a theoretical basis for effective protection and management of Gazella subguttrosa in Xinjiang.
Environmental factors play an important role in the evolution and genetic variation of species.To clarify the relationship between genetic diversity and environmental factors of Gazella subgutturosa in Xinjiang, in this study the mitochondrial DNA cytochrome b gene(1 140 bp) and control region(1 100 bp) sequences of Gazella subgutturosa were analysed from 84 individuals in 11 populations from across the species' natural range within Xinjiang by using PCR and direct sequencing methods.The results showed that the Gazella subgutturosa population in Xinjiang seems to show high haplotype but low nucleotide diversity, these results indicated that the Gazella subgutturosa investigated in this study exhibited low levels of genetic diversity.The results revealed by the analysis of correlation between environmental factors and the genetic diversity of various groups showed that altitude, annual precipitation, annual temperature, population are the main environmental factors affecting the genetic diversity of Gazella subgutturosa in Xinjiang,among which altitude is the key environmental factor.Our results provide a theoretical basis for effective protection and management of Gazella subguttrosa in Xinjiang.
引文
Chang Z F,Luo M F,Liu Z J,Yang J Y,Xiang Z F,Li M.2012.Human influence on the population decline and loss of genetic diversity in a small and isolated population of Sichuan snub-nosed monkeys (Rhinopithecus roxellana).Genetica,140(4-6):105-114.
Chu H J,Jiang Z G,Lan W X,Wang C,Tao Y S,Jiang F.2008.Dietary overlap among kulan Equus hemionus,goitered gazelle Gazella subgutturosa and livestock.Acta Zoologica Sinica,54(6):941-954.(in Chinese)
Chu H J,Jiang Z G,Jiang F,Ge Y,Tao Y S,Li B.2009.Summer and winter bed-site selection by goitered gazelle (Gazella subgutturosa sairensis).Zoological Research,30(3):311-318.(in Chinese)
Crochet P A,Desmarais E.2000.Slow rate of evolution in the mitochondrial control region of gulls (aves:laridae).Molecular Biology Evolution,17(12):1797-1806.
Durmu ? M.2010.Determination of home range habitat selection of gazelles (Gazella subgutturosa) by GPS telemetry in ?anliurfa.Master thesis.Ankara:Middle East Technical University.
Freeland J R,Kirk H,Peterson S D.2011.Molecular Ecology.2nd ed.Oxford Wiley-Blackwell.
Hao G P,Wu Z Y,Chen M S,Cao M Q,Brunnel D,Pelletier G,Huang C L,Yang Q.2004.Single nuucleotide polymorphisms of arabidopsis thaliana correspond to drought tolerance.Journal of Agricultural Biotechnology,12(2):122-131.(in Chinese)
Hewitt G.2000.The genetic legacy of the quaternary ice ages.Nature,405:907-913.
Hoban S M,Schlarbaum S E,Brosi S L,Romero-Severson J.2012.A rare case of natural regeneration in butternut,a threatened forest tree,is parent and space limited.Conservation Genetics,13(6):1447-1457.
IPCC.2007.Summary for Policymakers of the Synthesis Report of the IPCC Fourth Assessment Report.Cambridge,UK:Cambridge University Press.
Irwin D M,Kocher T D,Wilson A C.1991.Evolution of the cytochrome b gene of mammals.Journal of Molecular Evolution,32(2):128-144.
IUCN SSC Antelope Specialist Group.2017.Gazella subgutturosa.The IUCN Red List of Threatened Species 2017:e.T8976A50187422.
Gao X Y,Xu K F,Yao J,Jia Z X.1996.The population structure of goitred gazelle in Xinjiang.Acta Theriologica Sinica,16(1):14-18.(in Chinese)
Kocher T D,Thomas W K,Meyer A,Edwards S V,P??bo S,Villablanca F X,Wilson A C.1989.Dynamics of mitochondrial DNA evolution in animals:amplification and sequencing with conserved primers.Proceedings of the National Academy of Sciences of the United States of America,86(16):6196-6200.
Kingswood S C,Blank D A.1996.Gazella subgutturosa.Mammal Species,518:1- 10.
Librado P,Rozas J.2009.Dnasp v5:a software for comprehensive analysis of DNA polymorphism data.Bioinformatics,25(11):1451-1452.
Liu K F,Song Q J,Zhang L J,Yang W B.1994.The chemical components study of wild animal horn in Xinjinag.Journal of Xinjiang University(Natural Science Edition),11(3):76-77.(in Chinese)
Urban M C.2008.Ecological genetics.In:Encyclopedia of Life Sciences (ELS).Wiley,Chichester.
Macdonald D,Norris S.2001.The New Encyclopedia of Mammals.Oxford:Oxford University Press.
Ning L Q.2016.Genetic diversity and population structure of the Gazella subgutturosa from Xingjiang based on microsatellite.Master thesis.Urumqi:Xinjiang University.(in Chinese)
Parmesan C.1996.Climate and species’ range.Nature,382:765-766.
Pereladova O B,Bahloul K,Sempéré A J,Soldatova N V,Schadilov U M,Prisiadznuk V E.1998.Influence of environmental factors on a population of goitered gazelles (Gazella subgutturosa subgutturosa güldenstaedt,1780) in semi-wild conditions in an arid environment:a preliminary study.Journal of Arid Environments,39(4):577- 591.
Qiao J F,Yang W K,Xu W X,Xia C J,Liu W,Blank D.2011.Social structure of goitered gazelles Gazella subgutturosa in Xinjiang,China.Pakistan Journal of Zoology,43(4):769-775.
Risalat T,Askar M,Rizihan A,Amila A,Mahmut H.2012.An improved method for DNA extraction from the faeces of red deer.Agricultural Science & Technology,13(4):698- 700,900.
Sun M J,Gao X Y,Ablimit A,Shao M Q.2002.Study trends about Gazella subgutturosa.Arid Zone Research,19(3):75-79.(in Chinese)
Sorokin P A,Soldatova N V,Lukarevskiy V S,Kholodova M V.2011.Genetic diversity and relations of the goitered gazelle (Gazella subgutturosa) groups from Uzbekistan,Turkmenistan,and Azerbaijan:analysis of the D-loop of mitochondrial DNA.Biology Bulletin,38(6):585-590.
Scheiner S M.1993.Genetics and evolution of phenotypic plasticity.Annual Review of Ecology & Systematics,24(1):35-68.
Shamshidin A,Azizjan N,Mahmut H.2018a.Low genetic diversity in goitered gazelle (Gazella subgutturosa) in Northwestern China reveal by mitochondrial DNA cytochrome b gene.Acta Zoologica Bulgarica,70(2):211-218.
Shamshidin A,Guzalnur Z,Subinuer A,Zuoriguli Y,Mahmut H.2018b.Phylogeny and genetic structure of goitered gazelle(Artiodactyla,Bovidae) in northwest China indicated by hypervariable mitochondrial control region.Systematics and Biodiversity,16(6):527-537.
Thomas C D,Cameron A,Green R E.2004.Extinction risk from climate change.Nature,427:145-148.
Tamura K,Stecher G,Peterson D,Filipski A,Kumar S.2013.Mega6:molecular evolutionary genetics analysis version 6.0.Molecular Biology & Evolution,30:2725-2729.
Thompson J D,Gibson T J,Plewniak F,Jeanmougin F,Higgins D G.1997.The clustal_x windows interface:flexible strategies for multiple sequence alignment aided by quality analysis tools.Nucleic Acids Research,25(24):4876-4882.
Vanhala T K,Rijn C P E V,Buntjer J,Stam P,Nevo E,Poorter H.2004.Environmental,phenotypic and genetic variation of wild barley (Hordeum spontaneum) from Israel.Euphytica,137(3):297-309.
Wang S.1998.China Red Data Book of Endangered Animals-Mammalia.Beijing:Science Press,322-323.(in Chinese)
Xu K F,Ren Z G,Gao X Y.1997.The resources and status of Asiatic wild ass and goitered gazelle in Kalamaili Mt.Reserve zone.Arid Zone Research,14(Supplement):17-22.(in Chinese)
Xu W X,Qiao J F,Liu W,Yang W K.2008.Food habits of goitered gazelle(Gazella subgutturoa sairensis) in northern Xinjiang.Acta Theriologica Sinica,28(3):280-286.(in Chinese)
Yang W K,Qiao J F,Yao J,Gao X Y.2005.Characteristics of foraging habitat of goitered gazelles (Gazella subgutturosa sairensis) in eastern Junggar Basin,Xinjiang.Acta Theriologica Sinica,25(4):355-360.(in Chinese)
Zhao J J,Zhao X X,Zhang W,Zhou B C,Zhang Z W.2008.Establishment of PCR sex identification system of rabbit early embryos.Modern Journal of Animal Husbandry and Veterinary Medicine,12:42-46.
Zhou R,Li J Q,Li Y,Shi L M,Fang F J,Liu N F.2012.Effect of environmental factors on the population genetic diversity in the rusty-necklaced partridge (Alectoris magna).Journal of Lanzhou University(Natural Sciences),48(2):80-85.(in Chinese)
Zhang D C.2009.Effects of temperature and salinity on the development of tortoise foot and its genetic diversity.Master thesis.Fu-zhou:Fujian Normal University.(in Chinese)
Zhang J P,Liu Z,Zhang B,Yin X Y,Wang L,Shi H N,Kang Y J.2015.Genetic diversity and taxonomic status of gymnocypris chilianensis based on the mitochondrial DNA cytochrome b gene.Genetics & Molecular Research,14(3):9253-9260.
宁礼群.2016.基于微卫星对新疆鹅喉羚遗传多样性和种群结构研究.乌鲁木齐:新疆大学硕士学位论文.
孙铭娟,高行宜,阿不力米提·阿不都卡迪尔,邵明勤.2002.鹅喉羚(Gazella subgutturosa)研究动态.干旱区研究,19(3):75-79.
刘奎钫,宋启军,张龙军,杨伟斌.1994.新疆野生动物角的成份分析.新疆大学学报(自然科学版),11(3):76-77.
许可芬,任志刚,高行宜.1997.卡拉麦里山保护区的蒙古野驴、鹅喉羚资源及生存现状.干旱区研究,14(增刊):17-22.
汪松.1998.中国濒危动物红皮书—兽类.北京:科学出版社,322-323.
初红军,蒋志刚,兰文旭,王臣,陶永善,蒋峰.2008.蒙古野驴、鹅喉羚和家畜的食物重叠.动物学报,54(6):941-954.
初红军,蒋志刚,蒋峰,葛炎,陶永善,李斌.2009.鹅喉羚夏季和冬季卧息地选择.动物学研究,30(3):311-318.
张殿彩.2009.温度和盐度对龟足发育的影响及其遗传多样性的研究.福州:福建师范大学硕士学位论文.
杨维康,乔建芳,姚军,高行宜.2005.新疆准噶尔盆地东部鹅喉羚采食地的特征.兽类学报,25(4):355-360.
周蓉,李佳琦,李铀,施丽敏,房峰杰,刘迺发.2012.环境因子对大石鸡种群遗传多样性的影响.兰州大学学报(自然科学版),48(2):80- 85.
郝岗平,吴忠义,陈茂盛,曹鸣庆,Brunnel D,Pelletier G,黄丛林,杨清.2004.拟南芥cbf4基因位点的单核苷酸多态性(SNP)变化与抗旱表型的相应性.农业生物技术学报,12(2):122- 131.
赵建军,赵兴绪,张伟,周佰成,张兆旺.2008.兔早期胚胎PCR性别鉴定体系的建立.现代畜牧兽医,12:42- 46.
高行宜,许可芬,姚军,贾泽信.1996.新疆鹅喉羚的种群结构.兽类学报,16(1):14-18.
徐文轩,乔建芳,刘伟,杨维康.2008.新疆北部鹅喉羚的食性分析.兽类学报,28(3):280-286.
Chu H J,Jiang Z G,Lan W X,Wang C,Tao Y S,Jiang F.2008.Dietary overlap among kulan Equus hemionus,goitered gazelle Gazella subgutturosa and livestock.Acta Zoologica Sinica,54(6):941-954.(in Chinese)
Chu H J,Jiang Z G,Jiang F,Ge Y,Tao Y S,Li B.2009.Summer and winter bed-site selection by goitered gazelle (Gazella subgutturosa sairensis).Zoological Research,30(3):311-318.(in Chinese)
Crochet P A,Desmarais E.2000.Slow rate of evolution in the mitochondrial control region of gulls (aves:laridae).Molecular Biology Evolution,17(12):1797-1806.
Durmu ? M.2010.Determination of home range habitat selection of gazelles (Gazella subgutturosa) by GPS telemetry in ?anliurfa.Master thesis.Ankara:Middle East Technical University.
Freeland J R,Kirk H,Peterson S D.2011.Molecular Ecology.2nd ed.Oxford Wiley-Blackwell.
Hao G P,Wu Z Y,Chen M S,Cao M Q,Brunnel D,Pelletier G,Huang C L,Yang Q.2004.Single nuucleotide polymorphisms of arabidopsis thaliana correspond to drought tolerance.Journal of Agricultural Biotechnology,12(2):122-131.(in Chinese)
Hewitt G.2000.The genetic legacy of the quaternary ice ages.Nature,405:907-913.
Hoban S M,Schlarbaum S E,Brosi S L,Romero-Severson J.2012.A rare case of natural regeneration in butternut,a threatened forest tree,is parent and space limited.Conservation Genetics,13(6):1447-1457.
IPCC.2007.Summary for Policymakers of the Synthesis Report of the IPCC Fourth Assessment Report.Cambridge,UK:Cambridge University Press.
Irwin D M,Kocher T D,Wilson A C.1991.Evolution of the cytochrome b gene of mammals.Journal of Molecular Evolution,32(2):128-144.
IUCN SSC Antelope Specialist Group.2017.Gazella subgutturosa.The IUCN Red List of Threatened Species 2017:e.T8976A50187422.
Gao X Y,Xu K F,Yao J,Jia Z X.1996.The population structure of goitred gazelle in Xinjiang.Acta Theriologica Sinica,16(1):14-18.(in Chinese)
Kocher T D,Thomas W K,Meyer A,Edwards S V,P??bo S,Villablanca F X,Wilson A C.1989.Dynamics of mitochondrial DNA evolution in animals:amplification and sequencing with conserved primers.Proceedings of the National Academy of Sciences of the United States of America,86(16):6196-6200.
Kingswood S C,Blank D A.1996.Gazella subgutturosa.Mammal Species,518:1- 10.
Librado P,Rozas J.2009.Dnasp v5:a software for comprehensive analysis of DNA polymorphism data.Bioinformatics,25(11):1451-1452.
Liu K F,Song Q J,Zhang L J,Yang W B.1994.The chemical components study of wild animal horn in Xinjinag.Journal of Xinjiang University(Natural Science Edition),11(3):76-77.(in Chinese)
Urban M C.2008.Ecological genetics.In:Encyclopedia of Life Sciences (ELS).Wiley,Chichester.
Macdonald D,Norris S.2001.The New Encyclopedia of Mammals.Oxford:Oxford University Press.
Ning L Q.2016.Genetic diversity and population structure of the Gazella subgutturosa from Xingjiang based on microsatellite.Master thesis.Urumqi:Xinjiang University.(in Chinese)
Parmesan C.1996.Climate and species’ range.Nature,382:765-766.
Pereladova O B,Bahloul K,Sempéré A J,Soldatova N V,Schadilov U M,Prisiadznuk V E.1998.Influence of environmental factors on a population of goitered gazelles (Gazella subgutturosa subgutturosa güldenstaedt,1780) in semi-wild conditions in an arid environment:a preliminary study.Journal of Arid Environments,39(4):577- 591.
Qiao J F,Yang W K,Xu W X,Xia C J,Liu W,Blank D.2011.Social structure of goitered gazelles Gazella subgutturosa in Xinjiang,China.Pakistan Journal of Zoology,43(4):769-775.
Risalat T,Askar M,Rizihan A,Amila A,Mahmut H.2012.An improved method for DNA extraction from the faeces of red deer.Agricultural Science & Technology,13(4):698- 700,900.
Sun M J,Gao X Y,Ablimit A,Shao M Q.2002.Study trends about Gazella subgutturosa.Arid Zone Research,19(3):75-79.(in Chinese)
Sorokin P A,Soldatova N V,Lukarevskiy V S,Kholodova M V.2011.Genetic diversity and relations of the goitered gazelle (Gazella subgutturosa) groups from Uzbekistan,Turkmenistan,and Azerbaijan:analysis of the D-loop of mitochondrial DNA.Biology Bulletin,38(6):585-590.
Scheiner S M.1993.Genetics and evolution of phenotypic plasticity.Annual Review of Ecology & Systematics,24(1):35-68.
Shamshidin A,Azizjan N,Mahmut H.2018a.Low genetic diversity in goitered gazelle (Gazella subgutturosa) in Northwestern China reveal by mitochondrial DNA cytochrome b gene.Acta Zoologica Bulgarica,70(2):211-218.
Shamshidin A,Guzalnur Z,Subinuer A,Zuoriguli Y,Mahmut H.2018b.Phylogeny and genetic structure of goitered gazelle(Artiodactyla,Bovidae) in northwest China indicated by hypervariable mitochondrial control region.Systematics and Biodiversity,16(6):527-537.
Thomas C D,Cameron A,Green R E.2004.Extinction risk from climate change.Nature,427:145-148.
Tamura K,Stecher G,Peterson D,Filipski A,Kumar S.2013.Mega6:molecular evolutionary genetics analysis version 6.0.Molecular Biology & Evolution,30:2725-2729.
Thompson J D,Gibson T J,Plewniak F,Jeanmougin F,Higgins D G.1997.The clustal_x windows interface:flexible strategies for multiple sequence alignment aided by quality analysis tools.Nucleic Acids Research,25(24):4876-4882.
Vanhala T K,Rijn C P E V,Buntjer J,Stam P,Nevo E,Poorter H.2004.Environmental,phenotypic and genetic variation of wild barley (Hordeum spontaneum) from Israel.Euphytica,137(3):297-309.
Wang S.1998.China Red Data Book of Endangered Animals-Mammalia.Beijing:Science Press,322-323.(in Chinese)
Xu K F,Ren Z G,Gao X Y.1997.The resources and status of Asiatic wild ass and goitered gazelle in Kalamaili Mt.Reserve zone.Arid Zone Research,14(Supplement):17-22.(in Chinese)
Xu W X,Qiao J F,Liu W,Yang W K.2008.Food habits of goitered gazelle(Gazella subgutturoa sairensis) in northern Xinjiang.Acta Theriologica Sinica,28(3):280-286.(in Chinese)
Yang W K,Qiao J F,Yao J,Gao X Y.2005.Characteristics of foraging habitat of goitered gazelles (Gazella subgutturosa sairensis) in eastern Junggar Basin,Xinjiang.Acta Theriologica Sinica,25(4):355-360.(in Chinese)
Zhao J J,Zhao X X,Zhang W,Zhou B C,Zhang Z W.2008.Establishment of PCR sex identification system of rabbit early embryos.Modern Journal of Animal Husbandry and Veterinary Medicine,12:42-46.
Zhou R,Li J Q,Li Y,Shi L M,Fang F J,Liu N F.2012.Effect of environmental factors on the population genetic diversity in the rusty-necklaced partridge (Alectoris magna).Journal of Lanzhou University(Natural Sciences),48(2):80-85.(in Chinese)
Zhang D C.2009.Effects of temperature and salinity on the development of tortoise foot and its genetic diversity.Master thesis.Fu-zhou:Fujian Normal University.(in Chinese)
Zhang J P,Liu Z,Zhang B,Yin X Y,Wang L,Shi H N,Kang Y J.2015.Genetic diversity and taxonomic status of gymnocypris chilianensis based on the mitochondrial DNA cytochrome b gene.Genetics & Molecular Research,14(3):9253-9260.
宁礼群.2016.基于微卫星对新疆鹅喉羚遗传多样性和种群结构研究.乌鲁木齐:新疆大学硕士学位论文.
孙铭娟,高行宜,阿不力米提·阿不都卡迪尔,邵明勤.2002.鹅喉羚(Gazella subgutturosa)研究动态.干旱区研究,19(3):75-79.
刘奎钫,宋启军,张龙军,杨伟斌.1994.新疆野生动物角的成份分析.新疆大学学报(自然科学版),11(3):76-77.
许可芬,任志刚,高行宜.1997.卡拉麦里山保护区的蒙古野驴、鹅喉羚资源及生存现状.干旱区研究,14(增刊):17-22.
汪松.1998.中国濒危动物红皮书—兽类.北京:科学出版社,322-323.
初红军,蒋志刚,兰文旭,王臣,陶永善,蒋峰.2008.蒙古野驴、鹅喉羚和家畜的食物重叠.动物学报,54(6):941-954.
初红军,蒋志刚,蒋峰,葛炎,陶永善,李斌.2009.鹅喉羚夏季和冬季卧息地选择.动物学研究,30(3):311-318.
张殿彩.2009.温度和盐度对龟足发育的影响及其遗传多样性的研究.福州:福建师范大学硕士学位论文.
杨维康,乔建芳,姚军,高行宜.2005.新疆准噶尔盆地东部鹅喉羚采食地的特征.兽类学报,25(4):355-360.
周蓉,李佳琦,李铀,施丽敏,房峰杰,刘迺发.2012.环境因子对大石鸡种群遗传多样性的影响.兰州大学学报(自然科学版),48(2):80- 85.
郝岗平,吴忠义,陈茂盛,曹鸣庆,Brunnel D,Pelletier G,黄丛林,杨清.2004.拟南芥cbf4基因位点的单核苷酸多态性(SNP)变化与抗旱表型的相应性.农业生物技术学报,12(2):122- 131.
赵建军,赵兴绪,张伟,周佰成,张兆旺.2008.兔早期胚胎PCR性别鉴定体系的建立.现代畜牧兽医,12:42- 46.
高行宜,许可芬,姚军,贾泽信.1996.新疆鹅喉羚的种群结构.兽类学报,16(1):14-18.
徐文轩,乔建芳,刘伟,杨维康.2008.新疆北部鹅喉羚的食性分析.兽类学报,28(3):280-286.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |