玉米小斑病叶片生化变化的红外光谱分析
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摘要
利用红外光谱技术分析感染小斑病的玉米叶片,获取了染病玉米叶片的分子结构信息,研究结果表明真菌病原体对叶片中的蛋白质结构有一定的影响。二维相关红外光谱分析结果显示,健康玉米叶片中蛋白质二级结构的β-折叠构象随着玉米叶片的生长代谢发生变化,而染病玉米叶片中发生变化的是β-转角构象。二维相关红外光谱可以揭示真菌病原体入侵时玉米叶片分子结构的变化情况,为玉米病害的防治提供参考。
The infrared spectroscopy method is employed to analyze the corn leaves infected by the southern corn leaf blight disease and the molecular structure information of infected corn leaves is obtained.The research results show that the fungal pathogens have certain effects on the protein structure of the leaves.The two-dimensional correlation infrared spectral analysis indicates that theβ-sheet conformation of the protein secondary structure in healthy corn leaves changes with the growth and metabolism of corn leaves,while in the infected corn leaves is the change of theβ-turn conformation.Two-dimensional correlation infrared spectroscopy can be used to reveal the change of molecular structures of corn leaves invaded by the fungal pathogens and provide a reference for the prevention and control of corn diseases.
The infrared spectroscopy method is employed to analyze the corn leaves infected by the southern corn leaf blight disease and the molecular structure information of infected corn leaves is obtained.The research results show that the fungal pathogens have certain effects on the protein structure of the leaves.The two-dimensional correlation infrared spectral analysis indicates that theβ-sheet conformation of the protein secondary structure in healthy corn leaves changes with the growth and metabolism of corn leaves,while in the infected corn leaves is the change of theβ-turn conformation.Two-dimensional correlation infrared spectroscopy can be used to reveal the change of molecular structures of corn leaves invaded by the fungal pathogens and provide a reference for the prevention and control of corn diseases.
引文
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[2] Xu X D,Liu Z H.A color atlas of maize diseases and pests Color atlas of maize diseases and pests[M].Beijing:China Agricultural Science and Technology Press,2009:71-75.徐秀德,刘志恒.玉米病虫害原色图鉴[M].北京:中国农业科学技术出版社,2009:71-75.
[3] Lu S W,Gillian Turgeon B,Edwards M C.A ToxAlike protein fromCochliobolus heterostrophus induces light-dependent leaf necrosis and acts as a virulence factor with host selectivity on maize[J].Fungal Genetics and Biology,2015,81:12-24.
[4] Xu T Y,Yang J,Sun X R,et al.Mid-infrared spectroscopy analysis combined with support vector machine for rapid discrimination of botanical origin of honey[J].Laser &Optoelectronics Progress,2018,55(6):063003.徐天扬,杨娟,孙晓荣,等.中红外光谱法结合支持向量机快速鉴别蜂蜜品种[J].激光与光电子学进展,2018,55(6):063003.
[5] Zhai Y C,Wang F,Chi Y,et al.Calculation and experimental research on infrared spectrum of dioxin molecule[J].Laser &Optoelectronics Progress,2018,55(8):083001.翟云楚,王飞,池涌,等.二噁英分子红外光谱的计算和实验研究[J].激光与光电子学进展,2018,55(8):083001.
[6] Yu S H,Sheng L,Zhang C Y,et al.Physiological response of Arundo donax to cadmium stress by Fourier transform infrared spectroscopy[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2018,198:88-91.
[7] Xu X L,Zheng Y Z,Chen X C,et al.Identification of cattail pollen,pine pollen and bee pollen by Fourier transform infrared spectroscopy and two-dimensional correlation infrared spectroscopy[J].Journal of Molecular Structure,2018,1167:78-81.
[8] Yan R, Chen J B, Sun S Q,et al. Rapid identification of Lonicerae japonicae Flos and Lonicerae Flos by Fourier transform infrared(FT-IR)spectroscopy and two-dimensional correlation analysis[J].Journal of Molecular Structure,2016,1124:110-116.
[9] Jiang Q H,Zhang C B,Yang J,et al.Investigation on structural changes of isotactic polypropylene mesophase in the heating process by using twodimensional infrared correlation spectroscopy[J].Chinese Chemical Letters,2015,26(2):197-199.
[10] Haq Q M I, Mabood F, Naureen Z,et al.Application of reflectance spectroscopies(FTIR-ATR&FT-NIR)coupled with multivariate methods for robust in vivo detection of begomovirus infection in papaya leaves[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2018,198:27-32.
[11] Huleihel M, Shufan E, Tsror L, et al.Differentiation of mixed soil-borne fungi in the genus level using infrared spectroscopy and multivariate analysis[J]. Journal of Photochemistry and Photobiology B:Biology,2018,180:155-165.
[12] Sharma N, Khajuria Y, Sharma J, et al.Microscopic,elemental and molecular spectroscopic investigations of root-knot nematode infested okra plant roots[J].Vacuum,2018,158:126-135.
[13] Shi Y M,Li D Y,Liu G,et al.Influence of protein and carbohydrate in maize leaf by infection of maize southern rust using FTIR[J].Acta Photonica Sinica,2014,43(9):0930003.时有明,李栋玉,刘刚,等.FTIR研究南方锈病侵染对玉米叶片中蛋白质和碳水化合物的影响[J].光子学报,2014,43(9):0930003.
[14] Gorgulu S T, Dogan M, Severcan F. The characterization and differentiation of higher plants by Fourier transform infrared spectroscopy[J].Applied Spectroscopy,2007,61(3):300-308.
[15] Ahmed J,Ruwaih N,Mulla M,et al.Effect of high pressure treatment on functional,rheological and structural properties of kidney bean protein isolate[J].LWT-Food Science and Technology,2018,91:191-197.
[16] Choong Y K,Lan J,Lee H L,et al.Differential identification of mushrooms sclerotia by IR macrofingerprint method[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2016,152:34-42.
[17] Rana R, Herz K, Bruelheide H,et al. Leaf attenuated total reflection Fourier transform infrared(ATR-FTIR)biochemical profile of grassland plant species related to land-use intensity[J].Ecological Indicators,2018,84:803-810.
[18] Noda I, Ozaki Y. Two-dimensional correlation spectroscopy-Applications in vibrational and optical spectroscopy[M].Chichester,UK:John Wiley &Sons,Ltd,2004.
[19] Grewal M K, Huppertz T, Vasiljevic T.FTIR fingerprinting of structural changes of milk proteins induced by heat treatment, deamidation and dephosphorylation[J].Food Hydrocolloids,2018,80:160-167.
[20] Wu Y Q,Zhang L P,Jung Y M,et al.Twodimensional correlation spectroscopy in protein science, a summary for past 20 years[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2018,189:291-299.
[21] Noda I. Vibrational two-dimensional correlation spectroscopy(2DCOS)study of proteins[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2017,187:119-129.
[22] Zhang J R,Martin J M,Balint-Kurti P,et al.The wheat puroindoline genes confer fungal resistance in transgenic corn[J].Journal of Phytopathology,2011,159(3):188-190.
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