二氧化氯对玉米乙醇发酵糟液中脱氧雪腐镰刀菌烯醇的降解
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摘要
脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)是造成粮食污染的主要原因之一。采用高效液相色谱-质谱联用(HPLC-MS)对DON含量进行检测分析,通过液体浸泡法,探究了ClO_2对DON纯品以及玉米乙醇发酵糟液中DON的降解效果。结果显示:DON降解率随ClO_2溶液浓度的增大以及处理时间的延长而提高,且pH值对DON降解有影响。当pH值为4. 5～5. 5时,600 mg/L ClO_2溶液对DON纯品处理12 h,降解率为28. 23%,并产生了2种降解产物;该方法同样适用于玉米乙醇发酵糟液中DON降解,600 mg/L的ClO_2溶液对玉米发酵糟液处理12 h,降解率达40. 70%,降解效果较好,对处理乙醇发酵工艺中的DON或其他真菌毒素具有一定的指导意义,为大规模生产中对DON的去除提供了新的思路。
Deoxynivalenol( DON) is one of the main causes of food contamination. High performance liquid chromatography-mass spectrometry( HPLC-MS) was used to analyze the DON content. The degradation effects of ClO_2 on pure DON and DON in corn ethanol fermentation broth were investigated. The results showed that the degradation rate of DON increased with increasing concentration of ClO_2 and prolonging treatment time. Besides,pH had important effects on DON degradation. At pH 4. 5-5. 5,the degradation rate of pure DON was 28. 23% by 600 mg/L ClO_2 for 12 h,and two degradation products were produced. This method was also applicable to degrade DON in corn ethanol fermentation broth and resulted better results,as the degradation rate reached 40. 70%. This study has guiding significance for treating DON or other mycotoxins during ethanol fermentation process and provides a new idea for removing DON during large-scale production.
Deoxynivalenol( DON) is one of the main causes of food contamination. High performance liquid chromatography-mass spectrometry( HPLC-MS) was used to analyze the DON content. The degradation effects of ClO_2 on pure DON and DON in corn ethanol fermentation broth were investigated. The results showed that the degradation rate of DON increased with increasing concentration of ClO_2 and prolonging treatment time. Besides,pH had important effects on DON degradation. At pH 4. 5-5. 5,the degradation rate of pure DON was 28. 23% by 600 mg/L ClO_2 for 12 h,and two degradation products were produced. This method was also applicable to degrade DON in corn ethanol fermentation broth and resulted better results,as the degradation rate reached 40. 70%. This study has guiding significance for treating DON or other mycotoxins during ethanol fermentation process and provides a new idea for removing DON during large-scale production.
引文
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[3] HEYNDRICKX E,SIOEN I,HUYBRECHTS B,et al.Human biomonitoring of multiple mycotoxins in the Belgian population:Results of the BIOMYCO study[J]. Environment International,2015,84(2):82-89.
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[12] LU M M,GUAN E Q,BIAN K. Effect of ozone treatment on deoxynivalenol and quality evaluation of ozonised wheat[J]. Food Additives&Contaminants,2015,32(4):544-553.
[13] SUN Chao,JI Jian,WU Songling,et al. Saturated aqueous ozone degradation of deoxynivalenol and its application in contaminated grains[J]. Food Control,2016,69:185-190.
[14] PARK B J,TAKATORI K,SUGITA K Y,et al. Degradation of mycotoxins using microwave-induced argon plasma at atmospheric pressure[J]. Sueface&Coatings Technology,2007,201(9):5 733-5 737.
[15] CHARLENE E W,LLOYD B B. Heat and p H alter the concentration of deoxynivalenol in an aqueous enviroment[J]. Food Protection,1998,61(3):365-367.
[16] MISHRA S,DIXIT S,DWIVEDI P D,et al. Influence of temperature and p H on the degradation of deoxynivalenol(DON)in aqueous medium:comparative cytotoxicity of DON and degraded product[J]. Food Additives&Contaminants Part A Chemistry Analysis Control Exposure&Risk Assessment,2014,31(1):121-131.
[17]程亮,伍松陵,沈晗,等.脱氧雪腐镰刀菌烯醇降解菌的筛选及鉴定[J].粮油食品科技,2013,21(5):95-97.
[18]阪田总一郎.二氧化氯消毒杀菌体系的特征及在食品制造现场的应用[J].中国洗涤用品工业,2016,1:12-18.
[19] SZABO J,MINAMYER S. Decontamination of biological agents from drinking water infrastructure:a literature review and summary[J]. International Journal of Environmental Research and Public Health,2014,72:119-123.
[20]马骏,晋日亚,郑璐,等.气体二氧化氯对谷物表面黄曲霉杀菌效果研究[J].粮食与油脂,2015,28(6):63-65.
[21]耿鹏飞,高贵田,薛敏.气体二氧化氯在果蔬杀菌保鲜方面的研究与应用[J].食品工业科技,2014,35(6):387-391.
[22] ERICKSON M C,ORTEGA Y R. Inactivation of protozoan parasites in food,water,and environmental systems[J]. Journal of Food Protection, 2006, 69(11):2 786-2 808.
[23]常晓娇,王军,孙长坡,等.原代霉菌毒素中二氧化氯降解的研究[J].中国粮油学报,2016,31(9):113-118.
[24] GB/T 23503—2009,食品中脱氧雪腐镰刀菌烯醇的测定免疫亲和层析净化高效液相色谱法[S].北京:中国标准出版社,2005.
[25] YOUNG J C,ZHU Honghui,ZHOU Ting. Degradation of trichothecene mycotoxins by aqueous ozone[J]. Food and Chemical Toxicology,2006,44(3):417-424.
[26] YOUNG J C. Reduction in levels of deoxynivalenol in contaminated corn by chemical and physical treatment[J]. Journal of Agricultural and Food Chemistry,1986,34(3):465-467.
[27] CHAENKO N V,KORNIENKO G V,SOKOLENKO V A,et al. Redox-mediated oxidation of cyclohexanone to adipic acid on oxide-nickel anode,with active forms of oxygen involved[J]. Russian Journal of Applied Chemistry,2014,87(4):444-449.
[28]邢其毅.基础有机化学[M].第四版.北京:北京大学出版社,2016.
[2] LARSEN J C,HUNT J,PERRIN I,et al. Workshop on trichothecenes with a focus on DON:summary report[R].Toxicolgy Letters,2004,153(1):1-22.
[3] HEYNDRICKX E,SIOEN I,HUYBRECHTS B,et al.Human biomonitoring of multiple mycotoxins in the Belgian population:Results of the BIOMYCO study[J]. Environment International,2015,84(2):82-89.
[4] VIDAL A,CANO-SANCHO G,MARINA S,et al. Multidetection of urinary ochratoxin A,deoxynivalenol and its metabolites:pilot time-course study and risk assessment in Catalonia,Spain[J]. World Mycotoxin Journal,2016,9(4):1-16.
[5] MURI S D,VANDER V H,BOON P E,et al. Comparison of human health risks resulting from exposure to fungicides and mycotoxins via food[J]. Food and Chemical Toxicology,2009,47(12):2 963-2 974.
[6] PESTKA J J. Mechanisms of deoxynivalenol-induced gene expression and apoptosis[J]. Food Additives and Contaminants,2008,25(9):1 128-1 140.
[7] GRAZIAN F,PUJOL A,NICOLETTI C,et al. The foodassociated ribotoxin deoxynivalenol modulates inducible NO synthase in human intestinal cell model[J]. Toxicological Sciences,2015,145(2):372-382.
[8] PESTKA J J,SMOLINSKI A T. Deoxynivalenol:Toxicology and potential effects on humans[J]. Journal of Toxicology and Environmental Health,Part B,Critical Reviews,2005,8(1):39-69.
[9] GUAN S,WANG J Y,LIU S,et al. Occurrence of mycotoxin in Chinese feed and raw materials during 2013[C].Collected Papers of International Mycotoxin Conference,2014.
[10] PIACENTINI K C,ROCHA L O,FINTES L C,et al.Mycotoxin analysis of industrial beers from Brazil:The influence of fumonisin. B1 and deoxynivalenol in beer quality[J]. Food Chemistry,2017,218:64-69.
[11]邹忠义,黄雯,李洪军.紫外光辐射对脱氧雪腐镰刀菌烯醇和T-2毒素的去除作用[J].食品科学,2015,36(19):7-11.
[12] LU M M,GUAN E Q,BIAN K. Effect of ozone treatment on deoxynivalenol and quality evaluation of ozonised wheat[J]. Food Additives&Contaminants,2015,32(4):544-553.
[13] SUN Chao,JI Jian,WU Songling,et al. Saturated aqueous ozone degradation of deoxynivalenol and its application in contaminated grains[J]. Food Control,2016,69:185-190.
[14] PARK B J,TAKATORI K,SUGITA K Y,et al. Degradation of mycotoxins using microwave-induced argon plasma at atmospheric pressure[J]. Sueface&Coatings Technology,2007,201(9):5 733-5 737.
[15] CHARLENE E W,LLOYD B B. Heat and p H alter the concentration of deoxynivalenol in an aqueous enviroment[J]. Food Protection,1998,61(3):365-367.
[16] MISHRA S,DIXIT S,DWIVEDI P D,et al. Influence of temperature and p H on the degradation of deoxynivalenol(DON)in aqueous medium:comparative cytotoxicity of DON and degraded product[J]. Food Additives&Contaminants Part A Chemistry Analysis Control Exposure&Risk Assessment,2014,31(1):121-131.
[17]程亮,伍松陵,沈晗,等.脱氧雪腐镰刀菌烯醇降解菌的筛选及鉴定[J].粮油食品科技,2013,21(5):95-97.
[18]阪田总一郎.二氧化氯消毒杀菌体系的特征及在食品制造现场的应用[J].中国洗涤用品工业,2016,1:12-18.
[19] SZABO J,MINAMYER S. Decontamination of biological agents from drinking water infrastructure:a literature review and summary[J]. International Journal of Environmental Research and Public Health,2014,72:119-123.
[20]马骏,晋日亚,郑璐,等.气体二氧化氯对谷物表面黄曲霉杀菌效果研究[J].粮食与油脂,2015,28(6):63-65.
[21]耿鹏飞,高贵田,薛敏.气体二氧化氯在果蔬杀菌保鲜方面的研究与应用[J].食品工业科技,2014,35(6):387-391.
[22] ERICKSON M C,ORTEGA Y R. Inactivation of protozoan parasites in food,water,and environmental systems[J]. Journal of Food Protection, 2006, 69(11):2 786-2 808.
[23]常晓娇,王军,孙长坡,等.原代霉菌毒素中二氧化氯降解的研究[J].中国粮油学报,2016,31(9):113-118.
[24] GB/T 23503—2009,食品中脱氧雪腐镰刀菌烯醇的测定免疫亲和层析净化高效液相色谱法[S].北京:中国标准出版社,2005.
[25] YOUNG J C,ZHU Honghui,ZHOU Ting. Degradation of trichothecene mycotoxins by aqueous ozone[J]. Food and Chemical Toxicology,2006,44(3):417-424.
[26] YOUNG J C. Reduction in levels of deoxynivalenol in contaminated corn by chemical and physical treatment[J]. Journal of Agricultural and Food Chemistry,1986,34(3):465-467.
[27] CHAENKO N V,KORNIENKO G V,SOKOLENKO V A,et al. Redox-mediated oxidation of cyclohexanone to adipic acid on oxide-nickel anode,with active forms of oxygen involved[J]. Russian Journal of Applied Chemistry,2014,87(4):444-449.
[28]邢其毅.基础有机化学[M].第四版.北京:北京大学出版社,2016.
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