顶空固相微萃取-气相色谱-质谱联用法检测刺苞南蛇藤挥发性成分

详细信息    查看全文 | 推荐本文 |

英文篇名：Analysis of Chemical Compounds of Volatile Components of Celastrus flagellaris Rupr. by SPME-GC-MS 作者：杨娜 ; 王涵 ; 刘俊丽 ; 刘金平 ; 李平亚 英文作者：YANG Na;WANG Han;LIU Jun-li;LIU Jin-ping;LI Ping-ya;Jilin University, Ginseng Innovative Drug Development Local Joint National Engineering Research Center; 关键词：刺苞南蛇藤 ; 挥发油 ; 顶空固相微萃取-气相色谱-质谱联用 英文关键词：Celastrus flagellaris Rupr.;;Volatile components;;SPME-GC-MS 中文刊名：TCYA 英文刊名：Special Wild Economic Animal and Plant Research 机构：吉林大学药学院人参创新药物国家地方联合工程研究中心; 出版日期：2019-06-10 出版单位：特产研究 年：2019 期：v.41;No.164 基金：吉林省重大科技成果转化项目(20160301003YY);; 吉林大学2018年博士研究生交叉学科科研资助计划(10183201847) 语种：中文; 页：TCYA201902020 页数：5 CN：02 ISSN：22-1154/S 分类号：84-88

摘要

利用顶空固相微萃取-气相色谱-质谱联用技术检测刺苞南蛇藤藤茎的挥发性成分,为刺苞南蛇藤的进一步开发和利用提供理论依据。采用顶空固相微萃取技术对刺苞南蛇藤的挥发性成分进行富集,应用气相色谱-质谱联用法对检测出的化学成分进行分离和鉴定,采用面积归一法确定各成分的相对含量。共检出挥发性成分105种,鉴定出其中的102个组分,占挥发油总量的96.40%。主要包括有机酸、烷烃、烯烃、醇、醛和芳香类化合物等;含量较高的分别是乙基己醇(12.26%)、正壬醛(5.68%)、己酸(5.05%)、正十四烷(3.64%)、正癸醛(3.09%)、新醇(3.02%)、正十五烷(2.94%)、苯甲醛(2.43%)、香叶基丙酮(2.18%)。刺苞南蛇藤挥发性成分丰富,具有很强的药用价值。
        The volatile components from CF were determined by solid phase microextraction-gas chromatography-mass spectrometry(SPME-GC-MS), and provide the theoretical basis for the further development and utilization of Celastrus flagellaris Rupr.(CF).The volatile components were extracted by headspace solid phase micro-extraction(HS-SPME) then separated and identified by gas chromatographymass spectrometry(GC-MS).The relative content of each component was determined by area normalization.105 compounds were detected from essential oils of tassel of carrot, and 102 of them were identified, which accounted for 96.40% of total flow area, mainly include organic acids, alkanes, alkenes, alcohols, aldehydes, aromatic and other compounds; the main constituents of the oil were Ethylhexanol(12.26%),n-Nonanal(5.68%), Caproic acid(5.05%), n-Tetradecane(3.64%), n-Decanal(3.09%), Octanol(3.02%), n-Pentadecane(2.94%), Phenylmethanal(2.43%), Geranylacetone(2.18%).There are abundent chemical constituents in the volatile oil of CF, which has a strong medicinal value.

引文

[1]国家中医药管理局.中华本草[M].上海:科学技术出版社,1999:167.
    [2]昆明植物研究所.中国高等植物插图手册[M].昆明:昆明植物研究所,1983:657.
    [3]江苏植物研究所.新华本草[M].上海:科学技术出版社,1988:305.
    [4]吉林中医药学院.中国长白山药用植物彩色图谱[M].北京:人民卫生出版社,1997:273.
    [5]Zhao X.M,Guo F.J,Li Y.M, et al. Two new lignans from Celastrus flagellaris Rupr[J].Journal of Asian Natural Products Research,2012,14:159-164.
    [6]Wang M.A,Wang M.,Liu J.,et al. The Chemicial Structure of Celastrine from Celastrus Flagellaris[J]. Chinese Chemical Letters,1995,(6):679-680.
    [7]Wang M.A,Liu J.,Chen F H.Study on sesquiterpenes from Celastrus flagellaris Rupr[J].Acta Pharmaceutica Sinica,1997,32:368-372.
    [8]Wang M.A,Wang M.,Liu J.,et al. Study on the chemical structure of a pair of epimers by 2D NMR[J].Chinese Journal of Magnetic Resonance, 1995,(12):567-572.
    [9]杨娜,李平亚,周柏松,等.刺苞南蛇藤微量元素的测定[J].广东微量元素科学,2016,23(11):20-22.
    [10]周珊,赵立文,马腾蛟,等.固相微萃取(SPME)技术基本理论及应用进展[J].现代科学仪器,2006,1(2):86-90.
    [11]Chen W,Zhou P,Wong-Moon K C,et al.Identification of volatile degradants in formulations containing sesame oil using SPME/GC/MS[J]. Journal of Pharmaceutical&Biomedical Analysis,2007,44(2):450-455.
    [12]黄永辉,钟海雁,李忠海.固相微萃取及其在食用植物油香气研究中的应用[J].食品研究与开发,2006,27(8):192-196.
    [13]党凯茹,韩笑,彭腾,等.食用土当归中有机酸研究进展[J].亚太传统医药,2018,(6):71-74.
    [14]汤喜兰,刘建勋,李磊.中药有机酸类成分的药理作用及在心血管疾病的应用[J].中国实验方剂学杂志,2012,18(5):243-246.