高效液相色谱法测定中药材氨基甲酸酯类农药残留

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英文篇名：Determination of N-Methyl Carbamate Pesticides in Traditional Chinese Herbal Medicines by HPLC 作者：郭新颖 ; 顾俊 ; 陈峰 ; 史玉坤 ; 杨梅桂 ; 戴志英 ; 杨清华 ; 谢超 英文作者：GUO Xin-ying;GU Jun;CHEN Feng;SHI Yu-kun;YANG Mei-gui;DAI Zhi-ying;YANG Qing-hua;XIE Chao;Nantong Center for Disease Control and Prevention; 关键词：中药材 ; 氨基甲酸酯农药 ; 高效液相色谱 ; 农药残留 英文关键词：Chinese herbal medicine;;carbamate pesticides;;HPLC;;pesticide residues 中文刊名：NYZZ 英文刊名：Agrochemicals 机构：南通市疾病预防控制中心; 出版日期：2019-05-10 出版单位：农药 年：2019 期：v.58 基金：南通市卫生和计划生育委员会科研课题专项(WKZL2018084) 语种：中文; 页：NYZZ201905014 页数：3 CN：05 ISSN：21-1210/TQ 分类号：45-47

摘要

[目的]建立测定中药材中N-甲基氨基甲酸酯类农药残留量的高效液相色谱-柱后衍生-荧光检测分析方法。[方法]样品经乙腈-水超声提取,经无水硫酸钠-PSA柱吸附净化,用液相色谱法进行定量测定。[结果]6种N-甲基氨基甲酸酯类农药标准曲线线性关系良好,相关系数r为0.996～0.999,检出限为0.01～0.02 mg/kg,在3个加标水平下,6种农药的平均回收率为83.3%～107.5%,相对标准偏差为2.5%～11.3%。[结论]该方法准确、快速、净化效果好,可满足农药残留的检测要求。
        [Aims] The aims were to establish a method for determination of N-methyl carbamate pesticide residues in Chinese herbal medicine by HPLC-post column derivatization-fluorescence detection. [Methods] The samples were extracted with acetonitrile-water, purified with anhydrous sodium sulfate-PSA, and quantified by HPLC. [Results] The linear relationship for six kinds of N-methyl carbamate pesticide was good, the correlation coefficient was 0.996-0.999,the limit of detection was 0.01-0.02 mg/kg, the average recoveries of the 6 pesticides at the 3 spiked levels were83.3-107.5%, the relative standard deviation was 2.5-11.3%. [Conclusions] The method is accurate, rapid, and has good purification effect, which can meet the requirements of pesticide residue detection.

引文

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