柚皮苷抑制低氧/复氧致大鼠心肌细胞系H9c2损伤

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英文篇名：Naringin inhibits hypoxia/ reoxygenation-induced injury of rat cardiomyocyte cell line H9c2 作者：刘丹 ; 费慧芝 ; 金良友 ; 宋红霞 ; 张永慧 英文作者：LIU Dan;FEI Hui-zhi;JIN Liang-you;SONG Hong-xia;ZHANG Yong-hui;Chongqing Three Gorges Medical College;Chongqing Engineering Research Center of Antineoplastic Natural Drugs;Chongqing Three Gorges University; 关键词：柚皮苷 ; GRP78 ; ATF6 ; 低氧/复氧损伤 ; 凋亡 英文关键词：naringin;;GRP78;;ATF6;;hypoxia-reoxygenation injury;;apoptosis 中文刊名：JCYL 英文刊名：Basic & Clinical Medicine 机构：重庆三峡医药高等专科学校;重庆市抗肿瘤天然药物工程技术研究中心;重庆三峡学院; 出版日期：2019-07-05 出版单位：基础医学与临床 年：2019 期：v.39 基金：重庆市卫生和计划生育委员会中医药科技项目(ZY201703077) 语种：中文; 页：JCYL201907011 页数：5 CN：07 ISSN：11-2652/R 分类号：60-64

摘要

目的研究柚皮苷(NAR)对H9c2低氧/复氧(H/R)损伤心肌细胞内质网应激的影响。方法将H9c2心肌细胞随机分为对照组(C组)、低氧/复氧组(H/R组)低氧4 h后复氧24 h、柚皮苷10、20和40μg/mL组。于低氧前6 h给予柚皮苷培养再行低氧4 h后复氧24 h。实验后TUNEL检测心肌细胞凋亡;Western blot检测GRP78、ATF6、Bax及Bcl-2蛋白表达。结果与对照组相比,H/R组细胞凋亡增加,GRP78、ATF6、Bax蛋白表达和Bax/Bcl-2比率显著上调,而Bcl-2蛋白表达显著下调(P<0.05);与H/R组比较,柚皮苷3个剂量组均可使细胞凋亡减少,GRP78、ATF6、Bax、Bax/Bcl-2比率下调,Bcl-2上调(P<0.05),尤其以柚皮苷20和40μg/mL组作用最显著。结论柚皮苷预处理可以降低细胞凋亡,其机制可能与抑制内质网应激有关。
        Objective To study the effect of naringin(NAR) on endoplasmic reticulum(ER) stress in cardiomyocytes injured by H9 c2 hypoxia/reoxygenation(H/R).Methods H9 c2 cardiomyocytes were randomly divided into three groups: control group, hypoxia/reoxygenation group(H/R) for 24 h after hypoxia for 4 h, naringin 10, 20 and 40 μg/mL groups.Naringin was added 6 h before exposed to hypoxia for 4 h and then reoxygenation for 24 h. TUNEL detected apoptosis of cardiomyocytes after the experiment. Western blot was used to detect the expression of GRP78,ATF6,Bax and Bcl-2.Results Compared with control group, the cell apoptosis was increased, the expression of GRP78, ATF6, Bax protein expression and Bax/Bcl-2 were remarkably up-regulated(P< 0.05), but Bcl-2 protein expression was remarkably down-regulated in H/R group. Compared with the H/R group, the cell apoptosis decreased; the expression of GRP78, ATF6, Bax and Bax/Bcl-2 were down-regulated(P< 0.05), but Bcl-2 was up-regulated in the groups with naringin three groups,especially the 20 and 40 μg/mL groups. Conclusions The pretreatment of naringin can reduce the apoptosis of myocardial cell. The mechanism may be related to the inhibition of the apoptotic pathway as a response to endoplasmic reticulum stress.

引文

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