菊苣根中菊苣多糖含量测定研究
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摘要
目的:建立合格的菊苣多糖含量测定方法。方法:采用苯酚-硫酸显色法在492.5 nm处测定菊苣总糖含量,DNS(3,5-二硝基水杨酸)显色法在475.5 nm处测定菊苣还原糖含量,计算得到菊苣多糖的含量。结果:菊苣总糖含量测定:D-果糖在15.3～35.7μg/mL范围内线性关系良好,回归方程为A=0.019,8C-0.089,3,r=0.999,7;菊苣还原糖含量测定:D-果糖在60～140μg/mL范围内线性关系良好,回归方程为A=0.006,1C-0.086,7,r=0.999,6。结论:本实验确定的菊苣总糖和菊苣还原糖的含量测定方法简便可行,重复性好,精密度、准确度高,可用于菊苣根中菊苣多糖的含量测定,以完善菊苣的质量标准。
Objective: To establish the mensuration of chicory polysaccharides content. Method: The total sugar content of chicory was determined at 492.5 nm by phenol-sulfuric acid colorimetry, the content of reducing sugar in chicory was determined at 475.5 nm by DNS colorimetry. The content of chicory polysaccharide was calculated. Results: D-Fructose in total polysaccharide has a good linearity in the range of 15.3 ～35.7 μg/mL,and the regression equation was A=0.019,8 C-0.089,3, r=0.999,7. D-Fructose in reducing sugar has a good linearity in the range of 60～140 μg/mL, and the regression equation was A=0.006,1 C-0.086,7, r=0.999,6. Conclusion: The method of determination of total polysaccharide and reducing sugar determined in this experiment is simple and effective, precise, reproducible, accurate, and it can be used for chicory polysaccharide content determination in chicory root, to improve the quality standard of chicory.
Objective: To establish the mensuration of chicory polysaccharides content. Method: The total sugar content of chicory was determined at 492.5 nm by phenol-sulfuric acid colorimetry, the content of reducing sugar in chicory was determined at 475.5 nm by DNS colorimetry. The content of chicory polysaccharide was calculated. Results: D-Fructose in total polysaccharide has a good linearity in the range of 15.3 ～35.7 μg/mL,and the regression equation was A=0.019,8 C-0.089,3, r=0.999,7. D-Fructose in reducing sugar has a good linearity in the range of 60～140 μg/mL, and the regression equation was A=0.006,1 C-0.086,7, r=0.999,6. Conclusion: The method of determination of total polysaccharide and reducing sugar determined in this experiment is simple and effective, precise, reproducible, accurate, and it can be used for chicory polysaccharide content determination in chicory root, to improve the quality standard of chicory.
引文
[1]刘勇民.维吾尔药志(上)[M].乌鲁木齐:新疆人民出版社,1999:47.
[2]国家药典委员会.中国药典(一部)[M].北京:中国医药科技出版社,2015:217.
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[5]庄红艳,张冰,刘小青,等.菊苣药材中菊苣多糖的含量测定研究[J].中成药,2011,33(1):114-117.
[6]吴洪新,呼天明,张存莉,等.菊苣菊粉提取与纯化研究[J].西北农林科技大学学报(自然科学版),2006,34(1):91-95.
[7] Takeo Kamada, M itsutoshi Nakajima, H iroshi Nabetani, et al. Availability of membrane technology for purifying and concentrating oligosaccha rides[J].European Food Research and Technology,2002,214(5):435-440.
[8]张惟杰.糖复合物生化研究技术[M].上海:上海科学技术出版社,1999.
[9]孙芸.苯酚-硫酸法测定菊苣根中总糖的含量[J].中国民族民间医药杂志,2008,17(4):12-13.
[10]杨亚丽.菊苣有效成分的提取工艺研究[D].西安:西北农林科技大学,2006.
[11]张青,张天民.苯酚-硫酸比色法测定多糖含量[J].山东食品科技,2004,6(7):17-18.
[2]国家药典委员会.中国药典(一部)[M].北京:中国医药科技出版社,2015:217.
[3]刘勇民.维吾尔药志(上册)[M].新疆:新疆人民出版社,2005:100.
[4]朱钦龙.果糖低聚糖在产蛋鸡中的应用[J].兽药饲料添加剂,1996,1(3):23-25.
[5]庄红艳,张冰,刘小青,等.菊苣药材中菊苣多糖的含量测定研究[J].中成药,2011,33(1):114-117.
[6]吴洪新,呼天明,张存莉,等.菊苣菊粉提取与纯化研究[J].西北农林科技大学学报(自然科学版),2006,34(1):91-95.
[7] Takeo Kamada, M itsutoshi Nakajima, H iroshi Nabetani, et al. Availability of membrane technology for purifying and concentrating oligosaccha rides[J].European Food Research and Technology,2002,214(5):435-440.
[8]张惟杰.糖复合物生化研究技术[M].上海:上海科学技术出版社,1999.
[9]孙芸.苯酚-硫酸法测定菊苣根中总糖的含量[J].中国民族民间医药杂志,2008,17(4):12-13.
[10]杨亚丽.菊苣有效成分的提取工艺研究[D].西安:西北农林科技大学,2006.
[11]张青,张天民.苯酚-硫酸比色法测定多糖含量[J].山东食品科技,2004,6(7):17-18.
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