富钾烤烟品种成熟期根系分泌物和根际细菌多样性特征分析
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摘要
【目的】研究不同钾吸收利用效率烤烟品种成熟期根系分泌物和根际细菌多样性差异,有助于了解烟草富钾品种成熟期高效吸收利用钾素的机理。【方法】选用富钾烤烟品种ND202和常规品种K326、NC89进行盆栽试验,分析了不同烤烟品种成熟期根际主要养分含量、根系分泌物和根际细菌多样性的差异。【结果】成熟期NC89和ND202根际土中的碱解氮和有效磷含量极显著低于K326,速效钾含量3个品种间差异极显著,且为ND202最低。成熟期ND202非根际土的碱解氮极显著高于K326和NC89,有效磷和速效钾含量3个品种间差异极显著且均为ND202最高。3个品种成熟期根系分泌物主要成分含量有差异,ND202根系分泌物有机酸含量占其已检测出根系分泌物总量的81.80%,比NC89高12.86%,比K326高15.98%。3个烤烟品种成熟期根际和非根际土壤主要细菌在门水平和属分类水平上相对丰度有较大差异。根际土壤中链霉菌属、气微菌属和Iamia属的相对丰度表现为ND202>K326>NC89,而芽孢杆菌属、硝化螺菌属、乳酸乳球属、Steroidobacter属、Flavisolibacter属和野村菌属的相对丰度则表现为ND202 In order to understand the mechanism of potassium uptake and utilization in potassium-enriched tobacco genotype, the diversity of root exudates and rhizosphere bacteria in flue-cured tobacco varieties with different potassium uptake and utilization efficiencies at mature stage were studied. Pot experiment was carried out with potassium-enriched flue-cured tobacco variety ND202 and conventional varieties K326 and NC89 as material to analyze differences in main nutrient contents, root exudates and rhizosphere bacterial diversity in rhizosphere soil at maturity stage. The content of available nitrogen and phosphorus in NC89 and ND202 rhizosphere soil was significantly lower than that of K326 at maturity stage. There existed extremely significant difference in available potassium content among all 3 varieties(ND202 ranked the lowest). The available nitrogen in ND202 non rhizosphere soil at maturity stage was significantly higher than that of K326 and NC89. Extremely significant difference also existed in contents of available phosphorus and potassium among all three varieties, with ND202 ranked the top. The contents of main components in root exudates of all 3 varieties were different. The content of organic acids in ND202 root exudates accounted for 81.80% of the total root exudates, which was 12.86% higher than that of NC89 and 15.98% higher than that of K326. Relative abundances of the main bacteria in rhizosphere and non rhizosphere soil of the3 flue-cured tobacco varieties were different at phylum and genus level. The relative abundances of Streptomyces, Aeromicrobium and Iamia in rhizosphere soils followed the order as ND202>K326>NC89; while the relative abundances of Bacillus, Nitrospira, Lactococcus,Steroidobacter, Flavisolibacter and Nonomuraea could be ranked as ND202
引文
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[18]于会泳,申国明,高欣欣.烟草根系分泌物的GC-MS检测[J].中国烟草学报,2013,19(04):64-72.YU Huiyong,SHEN Guoming,GAO Xinxin,et al.Determination of tobacco root exudates by GC-MS[J].ActaTabacariaSinica,2013,19(04):64-72.
[19]高欣欣,于会泳,张继光,等.烤烟根系分泌物的分离鉴定及对种子萌发的影响[J].中国烟草科学,2012,33(3):87-90.GAO Xinxin,YU Huiyong,ZHANG Jiguang,et al.Identification of chemical compositions of root exudates from flue-cured tobacco and their influence to seed germination[J].Chinese Tobacco Science,2012,33(3):87-90.
[20]鲍士旦.土壤农化分析[M].北京:中国农业出版社,2000,108-109.BAO Shidan.Soil and agro-chemical analysis methods[M].Beijing:China Agriculture Press,2000,108-109.
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[22]周冀衡,李永平,杨虹琦,等.不同基因型烟草根系分泌物对难溶性磷钾的活化效应[J].湖南农业大学学报(自然科学版),2005,31(3):276-280.ZHOU Jiheng,LI Yongping,YANG Hongqi,et al.Activated effect of root secretions in the different genotype tobacco to difficult dissolving phosphorus and potassium[J].Journal of Hunan Agricultural University(Natural Sciences),2005,31(3):276-280.
[23]韩助君,许杰,卫宣志,等.外源有机酸对烟草生理指标和土壤速效钾含量的影响[J].中国农业科技导报,2016,18(4):109-114.HAN Zhujun,XU Jie,WEI Xuanzhi,et al.Effect of exogenous organic acids on tobacco physiology index and soil potassium content[J].Journal of Agricultural Science and Technology,2016,18(4):109-114.
[24]丛日环,李小坤,鲁剑巍,等.低分子量有机酸对红壤和黄褐土钾素转化的影响[J].土壤学报,2009,46(3):532-536.CONG Rihuang,LI Xiaokun,LU Jianwei,et al.Effect of lowmolecular weitht organic acids on transformation of potassium in red soidand yellow cinnamon soil in South China[J].Acta Pedologica Sinica,2009,46(3):532-536.
[25]张小全,张鋆鋆,刘冰洋,等.富钾烤烟品种成熟期钾素代谢特征研究[J].植物营养与肥料学报,2018,24(02):519-527.ZHANG Xiaoquan,ZHANGYunyun,LIU Bingyang,et al.Characteristics of potassium metabolism in potassium-enriched flue-cured tobacco genotype at mature stage[J].Plant Nutrition and Fertilizer Science,2018,24(02):519-527.
[26]Edwards J,Johnson C,Santos-Medellín C,et al.Structure,variation,and assembly of the root-associated microbiomes of rice[J].Proceedings of the National Academy of Sciences,2015112:911-920.
[27]蔡秋华,左进香,李忠环,等.抗性烤烟品种根际微生物数量及功能多样性差异[J].应用生态学报,2015,26(12):3766-3772.CAI Qiuhua,ZUO Jinxiang,LI Zonghuan,et al.Difference of rhizosphere microbe quantity and functional diversity among three flue-cured tobacco cultivars with different resistance[J].Chinese Journal of Applied Ecology,2015,26(12):3766-3772.
[2]Eisenhauer N,Scheu S,Jousset A.Bacterial diversity stabilizes community productivity[J].PLoS ONE,2012,7:e34517.
[3]吴林坤,林向民,林文雄.根系分泌物介导下植物-土壤-微生物互作关系研究进展与展望[J].植物生态学报,2014,38(3):298-310.WU Linkun,LIN Xiangmin,LIN Wenxiong.Advances and perspective in research on plant-soil-microbe interactions mediated by root exudates[J].Chinese Journal of Plant Ecology,2014,38(3):298-310.
[4]Picard C,Baruffa E,Bosco M et al.Enrichment and diversity of plant-probiotic microorganisms in the rhizosphere of hybrid maize during four growth cycles[J].Soil Biology and Biochemistry,2008,40:106-115.
[5]Peiffer J A,Spor A,Koren O,et al.Diversity and heritability of the maize rhizosphere microbiome under field conditions[J].Proceedings of the National Academy of Sciences,2013 110,6548-6553.
[6]Emmett B D,Youngblut N D,Buckley D H,et al.Plant phylogeny and life history shape rhizosphere bacterial microbiome of summer annuals in an agricultural field[J].Frontiers in Microbiology,2017,8:e2414.
[7]Ellouze W,Hamel C,Vujanovic V,et al.Chickpea genotypes shape the soil microbiome and affect the establishment of the subsequent durum wheat crop in the semiarid North American Great Plains[J].Soil Biology and Biochemistry,2013,63:129-141.
[8]Bulgarelli D,Garrido-Oter R,Münch P C,et al.Structure and function of the bacterial root microbiota in wild and domesticated barley[J].Cell Host and Microbe,2015,17:392-403.
[9]Zancarini A,Mougel C,Terrat S,et al.Combining ecophysiological and microbial ecological approaches to study the relationship between Medicago truncatula genotypes and their associated rhizosphere bacterial communities[J].Plant and Soil,2013,365:183-199.
[10]Chaparro J M,Badri D V,Vivanco J M.Rhizosphere microbiome assemblage is affected by plant development[J].The ISME Journal2014,8:790-803.
[11]Gschwendtner S,Esperschütz J,Buegger F et al.Effects of genetically modified starch metabolism in potato plants on photosynthate fluxes into the rhizosphere and on microbial degraders of root exudates[J].FEMS Microbiology Ecology,2011,76:564-575.
[12]王戈,杨焕文,赵正雄,等.不同抗性烤烟品种根际微生物数量及多样性差异研究[J].植物营养与肥料学报,2012,18(2):451-458.WANG Ge,YANG Huanwen,ZHAO Zhengxiong,et al.Microbe quantity and functional diversity in rhizospheres of different cultivars of flue-cured tobacco[J].Plant Nutrition and Fertilizer Science,2012,18(2):451-458.
[13]杨铁钊,杨志晓,林娟,等.不同烤烟基因型根际钾营养和根系特性研究[J].土壤学报,2009,46(04):646-651.YANG Tiezhao,YANG Zhixiao,LIN Juan,et al.K nutrition in rhizosphere and characteristics of roots of flue-cured tobaccos different in genotype[J].Acta Pedologica Sinica,2009,46(04):646-651.
[14]杨铁钊,彭玉富.富钾基因型烤烟钾积累特征研究[J].植物营养与肥料学报,2006,12(5):750-753.YANG Tiezhao,PENG Yufu.Potassium accumulation characteristics of rich potassium genotypic flue-cured tobacco[J].Plant Nutrition and Fertilizer Science,2006,12(5):750-753.
[15]杨志晓,张小全,尚晓颍,等.富钾基因型烤烟根系形态和生理特性研究[J].西北植物学报,2009,29(03):555-560.YANG Zhixiao,ZHANG Xiaoquan,SHANG Xiaoying,et al.Morphological and physiological characteristics of root in potassium-enriched flue-cured tobacco genotype[J].Acta Botanica Boreali-Occidentalia Sinica,2009,29(03):555-560.
[16]代晓燕,苏以荣,魏文学,等.打顶对烤烟植株钾素代谢和钾离子通道基因表达的影响[J].中国农业科学,2009,42(3):854-861.DAI Xiaoyan,SU Yirong,WEI Wenxue,et al.Effects of topping on potassium metabolism and expression of potassium channels in tobacco plants[J].Scientia Agricultura Sinica,2009,42(3):854-861.
[17]Chen Y F,Wang Y,Wu W H,et al.Membrane transporters for nitrogen,phosphate and potassium uptake in plants[J].Journal of Integrative Plant Biology,2008,50(7):835-848.
[18]于会泳,申国明,高欣欣.烟草根系分泌物的GC-MS检测[J].中国烟草学报,2013,19(04):64-72.YU Huiyong,SHEN Guoming,GAO Xinxin,et al.Determination of tobacco root exudates by GC-MS[J].ActaTabacariaSinica,2013,19(04):64-72.
[19]高欣欣,于会泳,张继光,等.烤烟根系分泌物的分离鉴定及对种子萌发的影响[J].中国烟草科学,2012,33(3):87-90.GAO Xinxin,YU Huiyong,ZHANG Jiguang,et al.Identification of chemical compositions of root exudates from flue-cured tobacco and their influence to seed germination[J].Chinese Tobacco Science,2012,33(3):87-90.
[20]鲍士旦.土壤农化分析[M].北京:中国农业出版社,2000,108-109.BAO Shidan.Soil and agro-chemical analysis methods[M].Beijing:China Agriculture Press,2000,108-109.
[21]Ndour P M,Gueye M,Barakat M,et al.Pearl millet genetic traits shape rhizobacterial diversity and modulate rhizosphere aggregation[J].Frontiers in Plant Science,2017,8:1288.
[22]周冀衡,李永平,杨虹琦,等.不同基因型烟草根系分泌物对难溶性磷钾的活化效应[J].湖南农业大学学报(自然科学版),2005,31(3):276-280.ZHOU Jiheng,LI Yongping,YANG Hongqi,et al.Activated effect of root secretions in the different genotype tobacco to difficult dissolving phosphorus and potassium[J].Journal of Hunan Agricultural University(Natural Sciences),2005,31(3):276-280.
[23]韩助君,许杰,卫宣志,等.外源有机酸对烟草生理指标和土壤速效钾含量的影响[J].中国农业科技导报,2016,18(4):109-114.HAN Zhujun,XU Jie,WEI Xuanzhi,et al.Effect of exogenous organic acids on tobacco physiology index and soil potassium content[J].Journal of Agricultural Science and Technology,2016,18(4):109-114.
[24]丛日环,李小坤,鲁剑巍,等.低分子量有机酸对红壤和黄褐土钾素转化的影响[J].土壤学报,2009,46(3):532-536.CONG Rihuang,LI Xiaokun,LU Jianwei,et al.Effect of lowmolecular weitht organic acids on transformation of potassium in red soidand yellow cinnamon soil in South China[J].Acta Pedologica Sinica,2009,46(3):532-536.
[25]张小全,张鋆鋆,刘冰洋,等.富钾烤烟品种成熟期钾素代谢特征研究[J].植物营养与肥料学报,2018,24(02):519-527.ZHANG Xiaoquan,ZHANGYunyun,LIU Bingyang,et al.Characteristics of potassium metabolism in potassium-enriched flue-cured tobacco genotype at mature stage[J].Plant Nutrition and Fertilizer Science,2018,24(02):519-527.
[26]Edwards J,Johnson C,Santos-Medellín C,et al.Structure,variation,and assembly of the root-associated microbiomes of rice[J].Proceedings of the National Academy of Sciences,2015112:911-920.
[27]蔡秋华,左进香,李忠环,等.抗性烤烟品种根际微生物数量及功能多样性差异[J].应用生态学报,2015,26(12):3766-3772.CAI Qiuhua,ZUO Jinxiang,LI Zonghuan,et al.Difference of rhizosphere microbe quantity and functional diversity among three flue-cured tobacco cultivars with different resistance[J].Chinese Journal of Applied Ecology,2015,26(12):3766-3772.
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