Thermostable ethanol tolerant xylanase from a cold-adapted marine species Acinetobacter johnsonii

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英文篇名：Thermostable ethanol tolerant xylanase from a cold-adapted marine species Acinetobacter johnsonii 作者：Dongsheng ; Xue ; Xuhao ; Zeng ; Dongqiang ; Lin ; Shanjing ; Yao 英文作者：Dongsheng Xue;Xuhao Zeng;Dongqiang Lin;Shanjing Yao;Key Laboratory of Fermentation Engineering (Hubei Universty of Technology), Ministry of Education, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology;Department of Chemical and Bioengineering, Zhejiang University; 英文关键词：Xylanase;;Ethanol tolerant;;Thermostable;;Cold-adapted;;Acinetobacter Johnsonii 中文刊名：ZHGC 英文刊名：中国化学工程学报(英文版) 机构：Key Laboratory of Fermentation Engineering (Hubei Universty of Technology), Ministry of Education, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology;Department of Chemical and Bioengineering, Zhejiang University; 出版日期：2019-05-15 出版单位：Chinese Journal of Chemical Engineering 年：2019 期：v.27 基金：Supported by the Science and Technology Research Project of Hubei Provincial Department of Education(B2017041) 语种：英文; 页：ZHGC201905022 页数：5 CN：05 ISSN：11-3270/TQ 分类号：196-200

摘要

A xylanase-producing bacterium, isolated from deep sea sediments, was identified as the cold-adapted marine species Acinetobacter Johnsonii. A cold-adapted marine species Acinetobacter Johnsonii could grow at 4 ℃. The optimum temperature and pH of xylanase from a cold-adapted marine species Acinetobacter Johnsonii were 55 ℃ and pH 6.0. Xylanase from a cold-adapted marine species Acinetobacter Johnsonii remained at 80% activity after incubation for 1 h at 65 ℃. The xylanase activity was 1.2-fold higher in 4% ethanol solution than in ethanol free solution. Gibbs free energy of denaturation, ΔG, was higher in 4% ethanol solution than in ethanol free solution. Thermostable ethanol tolerant xylanase was valuable for bioethanol production by simultaneous saccharification and fermentation process with xylan as a carbon source.
        A xylanase-producing bacterium, isolated from deep sea sediments, was identified as the cold-adapted marine species Acinetobacter Johnsonii. A cold-adapted marine species Acinetobacter Johnsonii could grow at 4 ℃. The optimum temperature and pH of xylanase from a cold-adapted marine species Acinetobacter Johnsonii were 55 ℃ and pH 6.0. Xylanase from a cold-adapted marine species Acinetobacter Johnsonii remained at 80% activity after incubation for 1 h at 65 ℃. The xylanase activity was 1.2-fold higher in 4% ethanol solution than in ethanol free solution. Gibbs free energy of denaturation, ΔG, was higher in 4% ethanol solution than in ethanol free solution. Thermostable ethanol tolerant xylanase was valuable for bioethanol production by simultaneous saccharification and fermentation process with xylan as a carbon source.

引文

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