摘要
小球藻是一种重要的资源微藻,它在水产养殖、生物能源生产和功能型保健食品开发领域有广泛的应用,因此开展小球藻集约化培养及养殖环境优化具有十分重要的意义。藻际共栖细菌产生的群感信号分子N-酰基-高丝氨酸内酯类化合物(N-acyl-homoserine lactones,AHLs)不仅可以调控细菌的生物学功能,还能影响藻类的生命活动。采用批次培养的方法,研究了100、200、300nmol·L~(-1)C_(10)-HSL(N-癸酰-L-高丝氨酸内酯)对普通海水小球藻(Chlorella vulgaris)PSⅡ光化学活性与光合作用关键酶的影响。结果显示:在C_(10)-HSL作用下,小球藻生长受到促进,细胞密度显著升高,而且存在着随C_(10)-HSL浓度上升促进作用增强的剂量-效应关系;小球藻PSⅡ光化学活性指标——最大光能转化效率Fv/Fm、实际光能转化效率Yeild及表观光合电子传递效率ETR明显提高,而且C_(10)-HSL对Fv/Fm的作用强于对Yeild的影响;小球藻光合作用关键限速酶——核酮糖-1,5-二磷酸羧化酶/加氧酶Rubisco、磷酸丙糖异构酶TPI、焦磷酸:果糖-6-磷酸-1-磷酸转移酶PFP、果糖-1,6-二磷酸醛缩酶FDA活性均不同程度上升,表明小球藻光合固碳及合成糖类的能力有所增强。
As an excellent resource microalgae, Chlorellahas wide applications for aquaculture, production of biofuel and development of functional food. Therefore, it is very important to develop the intensive cultivation pattern and optimize the breeding environment of Chlorella. AHLs(N-acyl-homoserine lactones)are knownas the quorum sensing signal molecule produced by commensalism bacteria in phycosphere, and playing important roles in not only regulating the biological function of bacteria, but also affecting the life activies of algae.A laboratorystudy was conducted to evaluate the effects of 100, 200, 300nmol·L~(-1)C_(10)-HSL(N-decanoyl-L-homoserine lactones)on PSⅡphotochemistry activity and photosynthesis crucial enzymes of the marine microalgae Chlorella vulgaris, in the photoautotrophic culture process. The results were as follows: treated with C_(10)-HSL, the growth of C. vulgariswas accelerated with the cell density increasing significantly. Moreover, there existed one dose-effect relationship between the C_(10)-HSL dose and the acceleration effect on growth of C. vulgaris; PSⅡ photochemistry activity indicators — Fv/Fm(maximal photochemical efficiency), Yeild(actual photochemical efficiency) andE TR(apparent photosynthetic electron transport ratio) increased obviously. However, there was a higher effect on Yeild than Fv/Fm treated by C_(10)-HSL; the photosynthesis crucial enzymes—Rubisco(ribulose bisphosphate carboxylase oxygenase), TPI(triose-phosphate isomerase), PFP(pyrophosphate: fructose-6-phosphate-1-phosphoric acid transferase) and FDA(fructose-1,6-bisphosphate aldolase) went up significantly, so it indicated that the photosynthetic carbon fixation and carbohydrate synthesis were enhanced.
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