摘要
目的:研究明日叶查尔酮对小鼠肝癌细胞新血管内皮生长因子(VEGF)和缺氧诱导因子-1α(HIF-1α)的影响。方法:将50只皮下接种肝癌H22细胞株的小鼠随机分为5组,每组10只。高、低剂量组分别每日经口灌胃给予50和5mg/kg的查尔酮,恩度组给予等量生理盐水灌胃,并腹腔注射恩度4mg/kg,空白对照组及阴性对照组则分别给予等量的生理盐水灌胃及腹腔注射,连续10d。用免疫组化法检测各组肝癌细胞VEGF的表达水平,用ELISA法检测血清中缺氧诱导因子-1α(HIF-1α)含量。结果,高剂量组及协同组中阳性表达细胞数目少且颜色浅,呈现弱表达,阳性表达率分别31.12%和12.22%,明显低于肿瘤对照组的56.26%(P<0.05)。阴性对照组和高剂量明日叶查尔酮组HIF-1α含量分别为88.92+11.73ng/L和77.35±11.39ng/L,差异均有统计学意义(P<0.05)。结论:明日叶查尔酮具有降低VEGF蛋白表达水平、HIF-1α浓度作用,说明它通过抑制肿瘤新生毛细血管的生成,产生抑癌作用。
To study the effect of Angelica keiskei chalcone(AC) on vascular endothelial growth factor(VEGF) and Hypoxia-inducible factor-1α(HIF-1α) expression on mice hepatocarcinoma cells.Fifty mice were inoculated with hepatocarcinoma H22 cells and divided into 5 groups.Group one to three were administered orally with AC by 5,25 and 50mg/kg/d,respectively.Group four was given Endostar 4mg/kg/d by intraperitoneal injection and tumor control group(group five) was given with normal saline.All mice were sacrificed after 10 days.The levels of the VEGF protein expression of hepatocarcinoma cells was determined by immunohistochemical method,and the concentration of the HIF-1α were detected by enzyme-linked immunosorbent assay(ELISA).The high dose group and positive group together a small number of cells and light color,showing weak expression,the positive expression rates were 31.12%and 12.22%,significantly lower than the tumor control group56.26%(P < 0.05).The negative control group and high dose of Ashitaba chalcone group HIF-1 alpha content were 88.92 + 11.73ng/L and 77.35 + 11.39ng/L,the differences were statistically significant(P < 0.05).Conclusion:Angelica keiskei chalcone has reduced the expression level of VEGF protein HIF-1 concentrations,indicating that it through inhibiting the formation of tumor angiogenesis,tumor suppressor function generation.
引文
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