摘要
Objective The detection of pathogenic bacteria in urine is an important criterion for diagnosing urinary tract infections(UTIs).By using fluorescence in situ hybridization(FISH) with rRNA-targeted,fluorescently labeled oligonucleotide probes,bacterial pathogens present in urine samples were identified within 3-4 h.In this study,three probes that are specific for Escherichia coli,Enterococcus faecalis and Staphylococcus aureus were designed based on the conserved 16 SRNA sequences,whereas probe Eub338 broadly recognizes all bacteria.Results and Discussion The samples for these experiments included 76 E.coli infected samples,32 E.faecalis infected samples and 9 S.aureus infected samples.Compared to conventional methods of bacterial identification,the FISH method produced positive results for > 90%of the samples tested.Fig.1.Fluorescent microscopy(magnification,×100) of microorganisms from urine samples after FISH.Part A:FISH with probes specific for Escherichia coli(green signal);Part B:FISH with probes specific for Enterococcus faecalli(red signal);Part C:FISH with probes specific for Staphylococcus aureus(green signal).Conclusions FISH has the potential to become an extremely useful diagnostic tool for UTIs because it has a quick turnaround time and high accuracy.Acknowledgements This work has been supported by "the Fundamental Research Funds for the Central Universities"
Objective The detection of pathogenic bacteria in urine is an important criterion for diagnosing urinary tract infections(UTIs).By using fluorescence in situ hybridization(FISH) with rRNA-targeted,fluorescently labeled oligonucleotide probes,bacterial pathogens present in urine samples were identified within 3-4 h.In this study,three probes that are specific for Escherichia coli,Enterococcus faecalis and Staphylococcus aureus were designed based on the conserved 16 SRNA sequences,whereas probe Eub338 broadly recognizes all bacteria.Results and Discussion The samples for these experiments included 76 E.coli infected samples,32 E.faecalis infected samples and 9 S.aureus infected samples.Compared to conventional methods of bacterial identification,the FISH method produced positive results for > 90%of the samples tested.Fig.1.Fluorescent microscopy(magnification,×100) of microorganisms from urine samples after FISH.Part A:FISH with probes specific for Escherichia coli(green signal);Part B:FISH with probes specific for Enterococcus faecalli(red signal);Part C:FISH with probes specific for Staphylococcus aureus(green signal).Conclusions FISH has the potential to become an extremely useful diagnostic tool for UTIs because it has a quick turnaround time and high accuracy.Acknowledgements This work has been supported by "the Fundamental Research Funds for the Central Universities"
引文