摘要
目的： 建立外周血单核细胞中谷胱甘肽（GSH）含量的测定方法及正常参考
值，并探讨鼻咽癌患者外周血单核细胞中 GSH 含量与放射治疗近期疗效的关
系。
方法： 采用 Tietze 还原酶法检测 102 例不同性别、不同年龄的健康人外周
血单核细胞中 GSH 含量，并观察不同性别、不同年龄组的差别，同时检测 126
例鼻咽癌患者外周血单核细胞中 GSH 含量，观察正常人和鼻咽癌患者之间的
差异，以及不同临床分期的鼻咽癌患者的 GSH 含量差异，同时观察鼻咽癌患
者中 GSH 含量高于健康人群组均数值和低于该数值的两组病人鼻咽肿物的近
期疗效情况。
结果： 正常人群中外周血单核细胞内 GSH 含量男女分别为：(133.88±72.32)
×10-9mmol/L ，(141.51±58.98)×10-9mmol/L，男女值无明显差异（P>0.05），测
得各年龄段值：30 岁以下为(132.99±49.16)×10-9mmol/L，30 至 39 岁为
(129.00±68.41)×10-9mmol/L，40 至 49 岁为(132.67±47.25)×10-9mmol/L，50 至 59
岁为(152.17±73.72)×10-9mmol/L，60 岁以上为(150.84±104.27)×10-9mmol/L。
各年龄段 GSH 值无明显差异（P>0.05）。鼻咽癌患者与健康人外周血 GSH 含
量分别为(140.03±53.87)×10-9mmol/L 和(138.08±64.92)×10-9mmol/L，两者
无明显差异（P>0.05）。Ⅰ、Ⅱ、Ⅲ、Ⅳ期的鼻咽癌患者外周血 GSH 含量分别
为(135.66±50.74)×10-9mmol/L，(141.35±69.57)×10-9mmol/L，(149.55±
2
79.40)×10-9mmol/L，(136.29±69.31)×10-9mmol/L，含量无明显差异（P>0.05）。
鼻咽癌患者中 65 例大于 138×10-9mmol/L（A 组），61 例小于 138×10-9mmol/L
（B 组），两组放疗方法相同。放疗结束时两组鼻咽部肿物完全消退率和部分
消退率分别为 47.7％、52.3％和 70.5％、29.5％，有明显差异（P<0.05）。
结论： Tietze 还原酶法检测外周血单核细胞内的 GSH 含量所测结果可信。
GSH 与鼻咽癌的发生、发展无直接关系。鼻咽癌患者外周血单核细胞中 GSH
含量高于健康人群组均数值者放疗后肿瘤消退率低，反之则高，该指标可作
为预测鼻咽癌放射敏感性及检测内源性 GSH 合成抑制剂作用效果的参考指
标。
Object: To establish a test method about Glutathione(GSH) content peripheral
blood monocytes in healthy people and its reference date. To investigate the
clinical significance of GSH content of peripheral blood monocytes in patients
with nasopharyngeal carcinoma.
Methods: The GSH contents of periphetsl blood monocytes in 126 cases of
nasopharyngeal patients and 102 cases of healthy persons were determined by
,
Tietze methods. And test the GSH content in healthy people s blood in different
sexes and different ages .The difference in contents between nasopharyngeal
patients and healthy persons was observed and the recent regression of
nasopharyngeal tumor in nasopharyngeal carcinoma patients whose GSH content
was higher or lower than the average GSH contents of healthy persons was
analysed.
Results: The GSH contents of peripheral blood monocytes in men and women
were (133.88±72.32) ×10-9mmol/L and (141.51±58.98)×10-9mmol/L, respectively
(P>0.05); the GSH contents in younger than 30 years, 30~39 years, 40~49 years, 50~59
years and above 60 years were (132.99±49.16)×10-9mmol/L, (129.00±68.41)
×10-9mmol/L,(132.67±47.25)×10-9mmol/L,(152.17±73.72)×10-9mmol/L, (150.84±
104.27)×10-9mmol/L respectively (P>0.05). The GSH contents of peripheral
4
blood monocytes in nasopharyngeal carcinoma patients and healthy persons
were(140.03±53.87)×10-9mmol/L and(138.08±64.92)×10-9mmol/L, respectively.
There was no significant difference（P>0.05）. The GSH contents in state Ⅰ, Ⅱ,
,
Ⅲ, and Ⅳ nasopharyngeal carcinoma patients blood were (135.66±50.74)
×10-9mmol/L，(141.35±69.57)×10-9mmol/L，(149.55±79.40)×10-9mmol/L，
(136.29±69.31）×10-9mmol/L, respectively. There was no significant difference
（P>0.05）. The two groups accepted the same radiotherapy. After finished
radiotherapy, the complete regression rate and partial regression rate of
nasopharyngeal tumor of the two groups were 47.7％, 52.3％ and 70.5％,
29.5％, respectively（P<0.05）.
Conclusion: This method is simple and results are reliable. GSH cannot affect the
occurrence and development of Nasopharyngeal carcinoma. The recent regression
rate of nasopharyngeal tumor in the lower GSH content group is higher than the
higher GSH content group. The GSH content of peripheral blood monocytes can be
used as an index to forecast radiosensitivity of Nasopharyngeal carcinoma and use
GSH synthesis depressant to enhance the radiosensitivity.
引文
1. 谷铣之等.肿瘤放射治疗学.北京：北京医科大学中国协和医科大学
联合出版社，1993：443.
2. Biaglow JE, Varmes ME, Epp ER, et al. Role of glutathione in the
aerobic radiation response. Int J Radiat Oncol Phys 1989 16:1311.
3. 沈瑜，糜福顺.肿瘤放射生物学.北京：中国医药科技出版社，2001：
14－20.
4. Chapliin DJ, Durand RE, Oliye. Acute hypoxic in tumors:Implication
for modifiers of radiation effaces. Int J Radiat Oncol Biol Phys
1986;12:1279.
5. 郑秀龙，金一尊，沈瑜.肿瘤治疗增敏药，上海：上海科学技术文
献出版社，1996，13－19.
6. Wei W, Nazzareno N. Pharmacol Reviews, 1998, 50(3):335-352
7. LU S S . FASEB,1999,13(10): 1169-1180.
8. 孔肇路, 金一尊, 沈芝芬. 辐射增敏剂作用下 SGC7901 细胞内
GSH 含量变化与受照射剂量关系的初步研究. 辐射研究与辐射工
艺学报 2002V20N3 216-219.
9. 温放, 王晓宁. 一氧化氮及相关物质在卵巢肿瘤患者外周血中的检
测. 中国肿瘤临床 2001V28N8 572-575.
10. 吴长珠等. 骨肿瘤患者血液 LPO SOD GSH-PX 与GSH 临床价值的
初步探讨. 肿瘤防治研究 1994V21N1 38-40.
17
11. 关俊玲等. 癌组织中 GSH 含量测定. 实用肿瘤学杂志. 2002V16N2
110-111.
12. Taghian A, Suit H, Phil D et al. Int. J. Radiat, Oncol. Biol, Phys,
1992,23(1): 55.
13. 金一尊，丁立, 严敏芬. 细胞 GSH 含量与放射增敏研究. 辐射研究
与辐射工艺学报 1996V14N3 172-176.