MAPC/EPC性组织工程化静脉瓣
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摘要
采用生物学及工程学原理构建组织工程化静脉瓣有望能治愈下肢慢性静脉功能不全,具有良好的临床应用前景。本文将受体Beagle犬骨髓来源的成体多能祖细胞(multipotent adult progenitor cells,MAPC)和内皮祖细胞(endothelial progenitor cells,EPC)作为种子细胞,采用同种异体脱细胞静脉瓣细胞外基质材料作为支架,利用多点注射、加压灌注的方法将MAPC种植到支架内部,然后应用血管自动旋转脉动系统,使EPC黏附于支架的内表面及瓣膜的窦腔两面,即得到“MAPC/EPC性组织工程化静脉瓣”;并对其形态结构、生物力学特性做了进一步研究。然后采用端端吻合术将构建的组织工程化静脉瓣吻接到受体犬颈外静脉,观察其体内过程。本文包括五部分内容:MAPC的分离、培养及鉴定;EPC的分离、培养及鉴定;同种异体脱细胞静脉瓣支架的制各;MAPC/EPC性组织工程化静脉瓣体外构建;组织工程化静脉瓣的在体研究。
It is a promising method to construct tissue-engineered venous valves to treat chronic venous insufficiency. We developed a new, tissue-engineered venous valves. Advantages of such tissue-engineered venous valves could include reduced thrombogenicity and greater long-term durability. Using multipotent adult progenitor cells (MAPC) and endothelial progenitor cells(EPC) derived from bone marrow as seeding cells, and decallularised allogeneic venous valves as the scaffolds. Seeded MAPC into the walls of scaffolds, and EPC onto the surface. We called the complex of cells/scaffold—"MAPC/EPC tissue-engineered venous valves", and observed the morphous and structures. Tissue-engineered venous valves were replanted into the acceptor dog's external jugular vein with end-to-end anastomosis. The physiological dispositions of the tissue-engineered venous valves were observed. This paper included 5 sections: Characterization and differentiation of bone marrow-derived multipotent adult progenitor cells; characterization and differentiation of endothelial progenitor cells from canine bone marrow; fabrication of decellularized scaffold of venous valves; construction of tissue engineered venous valves with MAPC and EPC in vitro; investigation of tissue engineered venous valves in vivo.
It is a promising method to construct tissue-engineered venous valves to treat chronic venous insufficiency. We developed a new, tissue-engineered venous valves. Advantages of such tissue-engineered venous valves could include reduced thrombogenicity and greater long-term durability. Using multipotent adult progenitor cells (MAPC) and endothelial progenitor cells(EPC) derived from bone marrow as seeding cells, and decallularised allogeneic venous valves as the scaffolds. Seeded MAPC into the walls of scaffolds, and EPC onto the surface. We called the complex of cells/scaffold—"MAPC/EPC tissue-engineered venous valves", and observed the morphous and structures. Tissue-engineered venous valves were replanted into the acceptor dog's external jugular vein with end-to-end anastomosis. The physiological dispositions of the tissue-engineered venous valves were observed. This paper included 5 sections: Characterization and differentiation of bone marrow-derived multipotent adult progenitor cells; characterization and differentiation of endothelial progenitor cells from canine bone marrow; fabrication of decellularized scaffold of venous valves; construction of tissue engineered venous valves with MAPC and EPC in vitro; investigation of tissue engineered venous valves in vivo.
引文
[1]Makarova NP,Hmelniker SM,Lurie F.Does surgical correction of the superficial femoral vein valve change the course of varicose disease? J Vasc Surg,2001,33(2):361-368.
[2]Bry JD,Muto PA,O'Donnell TF,et al.The clinical and hemodynamic results after axillary-to-popliteal vein valve transplantation.J Vasc Surg,1995,21(1):110-119.
[3]Boudjemline Y,Bonnet D,Sidi D,et al.Ispercutaneous implantation of a bovine venous valve in the inferior vena cava a reliable technique to treat chronic venous insufficiency syndrome? Med Sci Monit,2004,10(3):BR61-66.
[4]de Borst GJ,Teijink JA,Patterson M,et al.A percutaneous approach to deep venous valve insufficiency with a new self-expanding venous frame valve.J Endovasc Ther,2003,10(2):341-349.
[5]Pavcnik D,Barry T,Hans A,et al.Percutaneous bioprosthetic venous valve:A long-term study in sheep.J Vasc Surg,2002,35(3):598-603.
[6]Kasimir MT,Weigel G,Sharma J,et al.The decellularized porcine heart valve matrix in tissue engineering:platelet adhesion and activation.Thromb Haemost,2005,94(3):562-567.
[7]Cebotari S,Mertsching H,Kallenbach K,et al.Construction of autologous human heart valves based on an acellular allograft matrix.Circulation,2002,106(12 Suppl 1):I63-68.
[8]Schnell AM,Hoerstrup SP,Zund G,et al.Optimal cell source for cardiovascular tissue engineering:venous vs.aortic human myofibroblasts.Thorac Cardiovasc Surg,2001,49(4):221-225.
[9]Matsumura G,Miyagawa-Tomita S,Shin'oka T,et al.First evidence that bone marrow cells contribute to the construction of tissue-engineered vascular autografts in vivo. Circulation, 2003,108(14):1729-1734.
[10]Amit M, Carpenter MK, Inokuma MS, et al. Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged period of culture. Dev Biol, 2000, 227(2):271-278.
[11] Jiang Y, Vaessen B, Lenvik T,et al. Multipotent progenitor cells can be isolated from postnatal murine bone marrow, muscle, and brain. Exp Hematol, 2002,30:896-904.
[12] Jiang Y, Jahagirdar BN, Reinhardt RL, et al. Pluripotency of mesenchymal stem cells derived from adult marrow. Nature, 2002,418:41-49.
[13] He H, Shirota T, Yasui H, et al. Canine endothelial progenitor cell-lined hybrid vascular graft with nonthrombogenic potential. J Thorac Cardiovasc Surg, 2003, 126(2):455-464.
[14]Fadini GP, Agostini C, Avogaro A. Characterization of endothelial progenitor cells. Biochem Biophys Res Commun, 2005, 336(1): 1-2.
[15]Pavcnik D, Kaufman J, Uchida B, et al. Second-generation percutaneous bioprosthetic valve: A short-term study in sheep. J Vasc Surg, 2004,40(6): 1223-1227.
[16]Teebken OE, Puschmann C, Aper T, et al. Tissue-engineered bioprosthetic venous valve: a long-term study in sheep. Eur J Vasc Endovasc Surg, 2003, 25(4):305-312.
[17]Gulbins HE, Pritisanac A, Uhlig A, et al. Seeding of Human Endothelial Cells on Valve Containing Aortic Mini-Roots: Development of a Seeding Device and Procedure. Ann Thorac Surg, 2005, 79(6):2119-2126.
[18] HSU SH, Tsai IJ, Lin DJ, et aL The effect of dynamic culture conditions on endothelial cell seeding and retention on small diameter polyurethane vascular grafts. Med Eng Phys, 2005, 27(3):267-272.
[19] Abilez O, Benharash P, Mehrotra M, et aL A Novel Culture System Shows that Stem Cells Can be Grown in 3D and Under Physiologic Pulsatile Conditions for Tissue Engineering of Vascular Grafts. J Surg Res, 2006,132(2): 170-178.
[20]Buttafoco L, Engbers-Buijtenhuijs P, Poot AA, et al Physical characterization of vascular grafts cultured in a bioreactor. Biomaterials, 2006, 27(11):2380-2389.
[1]Pittenger MF,Mackay AM,Beck SC,et al.Multilineage potential of adult human mesenchymal stem cells.Science,1999,284(5411):143-147.
[2]Jiang Y,Vaessen B,Lenvik T,et al.Multipotent progenitor cells can be isolated from postnatal murine bone marrow,muscle,and brain.Exp Hematol,2002,30(8):896-904.
[3]Jiang Y,Jahagirdar BN,Reinhardt RL,et al.Pluripotency of mesenchymal stem cells derived from adult marrow.Nature,2002,418(6893):41-49.
[4]幕宁,高毅,唐力军.双阴性选择法纯化培养人骨髓多能成体祖细胞.第一军医大学学报,2005,25(5):535-537.
[5]陈黎红,程桦,闵军,等.小鼠骨髓多能成体祖细胞的分离、培养及体外诱导分化为胰岛素分泌细胞的研究.中国病理生理杂志,2005,21(9):1797-1801.
[6]Tolar J,O'shaughnessy MJ,Panoskaltsis-Mortari A,et al.Host factors that impact the biodistribution and persistence of multipotent adult progenitor cells.Blood,2006,107(10):4182-4188.
[7]Grove JE,Bruscia E,Krause DS.Plasticity of bone marrow-derived stem cells.Stem Cells,2004,22(4):487-500.
[8]Jin Y,Fuller L,Ciancio G,et al.Antigen presentation and immune regulatory capacity of immature and mature-enriched antigen presenting(dendritic) cells derived from human bone marrow.Hum Immunol,2004,65(2):93-103.
[9]Serafini M,Dylla SJ,Oki M,et al.Hematopoietic reconstitution by multipotent adult progenitor cells:precursors to long-term hematopoietic stem cells.J Exp Med,2007,204(1):129-139.
[10]Reyes M,Li S,Foraker J,et al.Donor origin of multipotent adult progenitor cells in radiation chimeras.Blood,2005,106(10):3646-3649.
[11]Muguruma Y,Reyes M,Nakamura Y,et al.In vivo and in vitro differentiation of myocytes from human bone marrow-derived multipotent progenitor cells.Exp Hematol,2003,31(12):1323-1230.
[12]Schwartz RE,Reyes M,Koodie L,et al.Multipotent adult progenitor cells from bone marrow differentiate into funetional hepatocyte-like cells.J Clin Invest,2002,109(10):1291-1302.
[13]Ross JJ,Hong Z,Willenbring B,et al.Cytokine-induced differentiation of multipotent adult progenitor cells into functional smooth muscle cells.J Clin Invest.2006,116(12):3139-3149.
[14]Prosper F,Stroncek D,Verfaillie CM.Verfaillie.Phenotypic and functional characterization of long-term culture-initiating cells present in peripheral blood progenitor collections of normal donors treated with granulocyte colony-stimulating factor.Blood,1996,88(6):2033-42.
[1] Hur J,Yoon C H,Kim H S,et al.Characterization of two types of endothelial progenitor cells and their different contributions to neovasculogenesis. Arterioscler Thromb Vasc Biol, 2004,24 (2): 288-293.
[2] Nishiwaki Y, Yoshida M, Iwaguro H, et al. Endothelial E-selectin potentiates neovascularization via endothelial progenitor cell-dependent and -independent mechanisms. Arterioscler Thromb Vasc Biol, 2007, 27(3):512-518.
[3] Asahara T, Masuda H, Takahashi T, et al. Bone marrow origin of endothelial progenitor cells responsible for postnatal vasculogenesis in physiological and pathological neovascularization. Circ Res, 1999, 85(3): 221-228.
[4] Hill JM, Zalos G, Halcox JP, et al. Circulating endothelial progenitor cells, vascular function, and cardiovascular risk. N Engl J Med, 2003, 348(7):593-600.
[5] Bellik L, Ledda F, Parenti A. Morphological and phenotypical characterization of human endothelial progenitor cells in an early stage of differentiation.FEBS LETTERS, 2005,579(12): 2731-2736.
[6] Matsumura G, Miyagawa-Tomita S, Shin'oka T,et al. First evidence that bone marrow cells contribute to the construction of tissue-engineered vascular autografts in vivo. Circulation, 2003,108(14): 1729-1734.
[7] Asahara T, Murohara T, Sullivan A, et al. Isolation of putative progenitor endothelial cells for angiogenesis. Science, 1997, 275(5302):964-967.
[8] Lin Y, Weisdorf D, Solovey A, et al. Origins of circulating endothelial cells and endothelial outgrowth from blood. J Clin Invest, 2000,105(1):71-77.
[9] Reyes M, Dudek A, Jahagirdar B, et al. Origin of endothelial progenitors in human postnatal bone marrow. J Clin Invest, 2002,109(3):337-346.
[10]Wassmann S, Werner N, Czech T, et al. Improvement of endothelial function by systemic transfusion of vascular progenitor cells.Circ Res, 2006,99(8):e74-83.
[11]Popa E R, Harmsen M C, Tio R A, et al. Circulating CD34~+ progenitor cells modulate host angiogenesis and inflammation in vivo. J Mol Cell Cardiol, 2006, 41(1):86-96.
[12]Pardanaud L, Eichmann A. Identification, emergence and mobilization of circulating endothelial cells or progenitors in the embryo. Development. 2006, 133(13):2527-2537.
[13]Sharpe E E 3rd, Teleron AA, Li B, et al. The origin and in vivo significance of murine and human culture-expanded endothelial progenitor cells. Am J Pathol, 2006, 168(5): 1710-1721.
[14]Butzal M, Loges S, Schweizer M. et al. Rapamycin inhibits proliferation and differentiation of human endothelial progenitor cells in vitro. Exp Cell Res, 2004, 300(1):65-71.
[15] Inoue T, Sata M, Hikichi Y, et al. Mobilization of CD34"positive bone marrow-derived cells after coronary stent implantation: impact on restenosis. Circulation, 2007, 115(5):553-561.
[16]Murayama T, Tepper OM, Silver M, et al. Determination of bone marrow-derived endothelial progenitor cell significance in angiogenic growth factor-induced neovascularization in vivo. Exp Hematol, 2002, 30(8): 967-972.
[1]Kasimir MT,Weigel G,Sharma J,et al.The decellularized porcine heart valve matrix in tissue engineering:platelet adhesion and activation.Thromb Haemost,2005, 94(3):562-567.
[2]Seung-Woo Cho,Sang HL,Il-Kwon Kim,et al.Small-Diameter Blood Vessels Engineered With Bone Marrow-Derived Cells.Ann Surg,2005,241(3):506-515.
[3]J.Andrew McKeel,Soma,S.R.Banik,et al.Human arteries engineered in vitro.EMBO,2003,4(6):633-638.
[4]VandeVondele S.Voros J.Hubbell JA.RGD-grafted poly-L-lysine-graft-(polyethylene glycol) copolymers block non-specific protein adsorption while promoting cell adhesion.Biotechnol Bioeng,2003,82(7):784-790.
[5]Kim TG.Park TG.Biomimicking extracellular matrix:cell adhesive RGD peptide modlfied electrospun poly(D,L-lactic-co-glycolic acid) nanofiber mesh.Tissue Eng,2006,12(2):221-233.
[6]Matsumura G,Sachiko MT,Toshiharu S,et al.First Evidence That Bone Marrow Cells Contribute to the Construction of Tissue-Engineered Vascular Autografts In Vivo.Circulation,2003,108(14):1729-1734.
[7]Kallenbach K,Leyh RG,Lefik E,et al.Guided tissue regeneration:porcine matrix does not transmit PERV.Biomaterials,2004,25(17):3613.
[8]Schmidt CE,Baier JM.Acellular vascular tissues:natural biomaterials for tissue repair and tissue engineering.Biomaterials,2000,21(22):2215-31.
[9]SHEN G,TSUNG HC,WU CHF,et al.Tissue engineering of blood vessels with endothelial cells differentiated from mouse embryonic stem cells.Cell research,2003,13(5):335-341.
[10]Kerdjoudj H,Boura C,Marchal L,et al.Decellularized umbilical artery treated with thin polyelectrolyte multilayer films:potential use in vascular engineering.Biomed Mater Eng,2006,16(4 Suppl):S123-129.
[11]刘太华,张炎,姜宗来.组织工程血管脱细胞支架主要组织相容性复合体Ⅰ抗原性的免疫组化观察.第二军医大学学报,2004,25(1):72-74.
[12]McFetridge PS,Abe K,Horrocks M,et al.Vascular tissue engineering:bioreactor design considerations for extended culture of primary human vascular smooth muscle cells.ASAIO J,2007,53(5):623-630.
[13]Shimizu K,Ito A,Arinobe M,et al.Effective cell-seeding technique using magnetite nanoparticles and magnetic force onto decellularized blood vessels for vascular tissue engineering.J Biosci Bioeng,2007,103(5):472-478.
[14]Lopez-Soler RI,Brennan MP,Goyal A,et al.Development of a mouse model for evaluation of small diameter vascular grafts. J Surg Res, 2007,139(1):1-6.
[1]de Borst GJ,Teijink JA,Patterson M,et al.A percutaneous approach to deep venous valve insufficiency with a new self-expanding venous frame valve.J Endovasc Ther,2003,10(2):341-349.
[2]Boudjemline Y,Bonnet D,Sidi D,et al.Ispercutaneous implantation of a bovine venous valve in the inferior vena cava a reliable technique to treat chronic venous insufficiency syndrome? Med Sci Monit,2004,10(3):BR61-66.
[3]Bry JD,Muto PA,O'Donnell TF,et al.The clinical and hemodynamic results after axillary-to-popliteal vein valve transplantation.J Vasc Surg,1995,21(1):110-119.
[4]Makarova NP,Hmelniker SM,Lurie F.Does surgical correction of the superficial femoral vein valve change the course of varicose disease? J Vasc Surg,2001,33(2):361-368.
[5]Kucher T,Brener B,Marak M,et al.Endovascular delivery of vein segments with valves versus direct anastomosis.J Endovasc Ther.2005,12(3):366-70.
[6]Vijayan V,Shukla N,Johnson JL,et al.Long-term reduction of medial and intimal thickening in porcine saphenous vein grafts with a polyglactin biodegradable external sheath.J Vasc Surg.2004,40(5):1011-9.
[7]Lichtenberg A,Tudorache I,Cebotari SP,et al.Testing of Tissue-Engineered Heart Valves Re-Endothelialized Under Simulated Physiological Conditions.Circulation.2006,114(1 Suppl):I559-565.
[8]Kasimir MT,Weigel G,Sharma J,et al.The decellularized porcine heart valve matrix in tissue engineering:platelet adhesion and activation.Thromb Haemost,2005,94(3):562-567.
[9]Kazunori Shiminu,Akira Ito,Manabu Arinobe,et al.Effective cell-seeding technique using magnetite nanoparticles and magnetic force onto decellularized blood vessels for vascular tissue engineering.J Bios &Bioe.2007,103(5):472-478.
[10]Sandip Sarkar,Thomas SR,George Hamilton,et al.Achieving the ideal properties for vascular bypass grafts using a tissue engineered approach:a review.Med Bio Eng Comput.2007,45(4):327-336.
[11]Pavcnik D, Barry T, Hans A, et al. Percutaneous bioprosthetic venous valve: A long-term study in sheep. J Vasc Surg, 2002,35(3):598-603.
[12]Teebken OE, Puschmann C, Aper T, et al. Tissue-engineered bioprosthetic venous valve: a long-term study in sheep. Eur J Vasc Endovasc Surg, 2003, 25(4):305-312.
[13]Matsumura G, Miyagawa-Tomita S, Shin'oka T,et al. First Evidence That Bone Marrow Cells Contribute to the Construction of Tissue-Engineered Vascular Autografts In Vivo. Circulation. 2003,108(14):1729-1734.
[14]Asahara T, Murohara T, Sullivan A, et al. Isolation of putative progenitor endothelial cells for angiogenesis. Science. 1997, 275(5302):964-967.
[15]Tolar J, O'shaughnessy MJ, Panoskaltsis-Mortari A, et al. Host factors that impact the biodistribution and persistence of multipotent adult progenitor cells. Blood, 2006, 107(10): 4182-4188.
[16] Jiang Y, Jahagirdar BN, Reinhardt RL, et al. Pluripotency of mesenchymal stem cells derived from adult marrow. Nature, 2002, 418(6893):41-49.
[17] Schwartz RE, Reyes M, Koodie L, et al. Multipotent adult progenitor cells from bone marrow differentiate into functional hepatocyte-like cells. J Clin Invest, 2002, 109(10):1291-1302.
[18]Muguruma Y, Reyes M, Nakamura Y, et al. In vivo and in vitro differentiation of myocytes from human bone marrow-derived multipotent progenitor cells.Exp Hematol, 2003, 31(12):1323-1230.
[19]Wassmann S, Werner N, Czech T, et al. Improvement of endothelial function by systemic transfusion of vascular progenitor cells. Circ Res. 2006, 99(8):e74-83.
[20]Hur J, Yoon CH, Kim HS, et al.Characterization of two types of endothelial progenitor cells and their different contributions to neovasculogenesis. Arterioscler Thromb Vasc Biol. 2004, 24(2): 288-293
[21] Awad O, Dedkov EI, Jiao C, et al. Differential healing activities of CD34+ and CD14+ endothelial cell progenitors. Arterioscler Thromb Vase Biol. 2006, 26(4):758-64.
[22]Abilez O, Benharash P, Mehrotra M, et al. A Novel Culture System Shows that Stem Cells Can be Grown in 3D and Under Physiologic Pulsatile Conditions for Tissue Engineering of Vascular Grafts. J Surg Res, 2006,132(2): 170-178.
[23] Buttafoco L, Engbers-Buijtenhuijs P, Poot AA, et al. Physical characterization of vascular grafts cultured in a bioreactor. Biomaterials, 2006, 27(11):2380-2389.
[1]Boudjemline Y,Bonnet D,Sidi D,et al.Ispercutaneous implantation of a bovine venous valve in the inferior vena cava a reliable technique to treat chronic venous insufficiency syndrome? Med Sci Monit,2004,10(3):BR61-66.
[2]Makarova NP,Hmelniker SM,Lurie F.Does surgical correction of the superficial femoral vein valve change the course of varicose disease? J Vasc Surg,2001,33(2):361-368.
[3]Pavcnik D,Barry T,Hans A,et al.Percutaneous bioprosthetic venous valve:A long-term study in sheep.J Vasc Surg,2002,35(3):598-603.
[4]Pavcnik D,Kaufman J,Uchida B,et al.Second-generation percutaneous bioprosthetic valve:A short-term study in sheep.J Vasc Surg,2004,40(6):1223-1227.
[5]de Borst GJ,Teijink JA,Patterson M,et al.A percutaneous approach to deep venous valve insufficiency with a new self-expanding venous frame valve.J Endovasc Ther,2003,10(2):341-349.
[6]Teebken OE,Puschmann C,Aper T,et al.Tissue-engineered bioprosthetic venous valve:a long-term study in sheep.Eur J Vasc Endovasc Surg,2003,25(4):305-312.
[7]Thorpe PE,Osse FJ,Correa LO.The valve-stent:development of a percutaneous prosthesis for treatment of valvular insufficiency.Presented at the 12th Annual meeting of the American Venous Forum.2000,2:3-6.
[8]Pavcnik D,Uchida BT,Timmermans H,et al.The square stent-based large vessel occluder:an experimental pilot study.J Vasc Interv Radiol.2001,12(11):1353-1355.
[9] Teebken OE, Pichlmaier AM, Haverich A. Cell seeded decellularised allogeneic matrix grafts and biodegradable polydioxanone-prostheses compared with arterial autografts in a porcine model. Eur J Vasc Endovasc Surg 2001, 22(2): 139-145.
[1]Makarova NP,Hmelniker SM,Lurie F.Does surgical correction of the superficial femoral vein valve change the course of varicose disease? J Vasc Surg,2001,33(2):361-368.
[2]Bry JD,Muto PA,O'Donnell TF,et al.The clinical and hemodynamic results after axillary-to-popliteal vein valve transplantation.J Vasc Surg,1995,21(1):110-119.
[3]Boudjemline Y,Bonnet D,Sidi D,et al.Ispercutaneous implantation of a bovine venous valve in the inferior vena cava a reliable technique to treat chronic venous insufficiency syndrome? Med Sci Monit,2004,10(3):BR61-66.
[4]Kasimir MT,Weigel G,Sharma J,et al.The decellularized porcine heart valve matrix in tissue engineering:platelet adhesion and activation.Thromb Haemost,2005,94(3):562-567.
[5]Pavcnik D,Barry T,Hans A,et al.Percutaneous bioprosthetic venous valve:A long-term study in sheep.J Vasc Surg,2002,35(3):598-603.
[6]Pavcnik D,Kaufman J,Uchida B,et al.Second-generation percutaneous bioprosthetic valve:A short-term study in sheep.J Vasc Surg,2004,40(6):1223-1227.
[7]de Borst GJ,Teijink JA,Patterson M,et al.A percutaneous approach to deep venous valve insufficiency with a new self-expanding venous frame valve.J Endovasc Ther,2003,10(2):341-349.
[8]刘太华,张炎,姜宗来,等.组织工程血管脱细胞支架主要组织相容性复合体Ⅰ抗原性的免疫组化观察.第二军医大学学报,2004,25(1):72-74.
[9]Cebotari S,Mertsching H,Kallenbach K,et al.Construction of autologous human heart valves based on an acellular allograft matrix.Circulation,2002,106(12 Suppl 1):I63-68.
[10]Teebken OE,Puschmann C,Aper T,et al.Tissue-engineered bioprosthetic venous valve:a long-term study in sheep.Eur J Vasc Endovasc Surg,2003,25(4):305-312.
[11]董念国,叶晓峰,史嘉伟,等.组织工程瓣膜天然支架去细胞方法的比较.中华实验外科杂志,2005,22(3):377.
[12]Schnell AM,Hoerstrup SP,Zund G,et al.Optimal cell source for cardiovascular tissue engineering:venous vs.aortic human myofibroblasts.Thorac Cardiovasc Surg,2001,49(4):221-225.
[13]He H,Shirota T,Yasui H,et al.Canine endothelial progenitor cell-lined hybrid vascular graft with nonthrombogenic potential.J Thorac Cardiovasc Surg,2003,126(2):455-464.
[14]Fadini GP,Agostini C,Avogaro A.Characterization of endothelial progenitor cells.Biochem Biophys Res Commun,2005,336(1):1-2.
[15]Amit M,Carpenter MK,Inokuma MS,et al.Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged period of culture.Dev Biol,2000,227(2):271-278.
[16]Abilez O,Benharash P,Mehrotra M,et al.A Novel Culture System Shows that Stem Cells Can be Grown in 3D and Under Physiologic Pulsatile Conditions for Tissue Engineering of Vascular Grafts.J Surg Res,2006,132(2):170-178.
[17]Buttafoco L,Engbers-Buijtenhuijs P,Poot AA,et al.Physical characterization of vascular grafts cultured in a bioreactor.Biomaterials,2006,27(11):2380-2389.
[18]Gulbins HE,Pritisanac A,Uhlig A,et al.Seeding of Human Endothelial Cells on Valve Containing Aortic Mini-Roots:Development of a Seeding Device and Procedure.Ann Thorac Surg,2005,79(6):2119-2126.
[19]HSU SH,Tsai IJ,Lin DJ,et al.The effect of dynamic culture conditions on endothelial cell seeding and retention on small diameter polyurethane vascular grafts.Med Eng Phys,2005,27(3):267-272.
[20]Hench LL,Polak JM.Third-generation biomedical materials.Science,2002,295(5557):1014-1017.
[21]Ye O,Zund G,Benedikt P,et al.Fibrin gel as a three dimensional matrix in cardiovascular tissue engineering.Eur J Cardiothorac Surg,2000,17(5):587-591.
[2]Bry JD,Muto PA,O'Donnell TF,et al.The clinical and hemodynamic results after axillary-to-popliteal vein valve transplantation.J Vasc Surg,1995,21(1):110-119.
[3]Boudjemline Y,Bonnet D,Sidi D,et al.Ispercutaneous implantation of a bovine venous valve in the inferior vena cava a reliable technique to treat chronic venous insufficiency syndrome? Med Sci Monit,2004,10(3):BR61-66.
[4]de Borst GJ,Teijink JA,Patterson M,et al.A percutaneous approach to deep venous valve insufficiency with a new self-expanding venous frame valve.J Endovasc Ther,2003,10(2):341-349.
[5]Pavcnik D,Barry T,Hans A,et al.Percutaneous bioprosthetic venous valve:A long-term study in sheep.J Vasc Surg,2002,35(3):598-603.
[6]Kasimir MT,Weigel G,Sharma J,et al.The decellularized porcine heart valve matrix in tissue engineering:platelet adhesion and activation.Thromb Haemost,2005,94(3):562-567.
[7]Cebotari S,Mertsching H,Kallenbach K,et al.Construction of autologous human heart valves based on an acellular allograft matrix.Circulation,2002,106(12 Suppl 1):I63-68.
[8]Schnell AM,Hoerstrup SP,Zund G,et al.Optimal cell source for cardiovascular tissue engineering:venous vs.aortic human myofibroblasts.Thorac Cardiovasc Surg,2001,49(4):221-225.
[9]Matsumura G,Miyagawa-Tomita S,Shin'oka T,et al.First evidence that bone marrow cells contribute to the construction of tissue-engineered vascular autografts in vivo. Circulation, 2003,108(14):1729-1734.
[10]Amit M, Carpenter MK, Inokuma MS, et al. Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged period of culture. Dev Biol, 2000, 227(2):271-278.
[11] Jiang Y, Vaessen B, Lenvik T,et al. Multipotent progenitor cells can be isolated from postnatal murine bone marrow, muscle, and brain. Exp Hematol, 2002,30:896-904.
[12] Jiang Y, Jahagirdar BN, Reinhardt RL, et al. Pluripotency of mesenchymal stem cells derived from adult marrow. Nature, 2002,418:41-49.
[13] He H, Shirota T, Yasui H, et al. Canine endothelial progenitor cell-lined hybrid vascular graft with nonthrombogenic potential. J Thorac Cardiovasc Surg, 2003, 126(2):455-464.
[14]Fadini GP, Agostini C, Avogaro A. Characterization of endothelial progenitor cells. Biochem Biophys Res Commun, 2005, 336(1): 1-2.
[15]Pavcnik D, Kaufman J, Uchida B, et al. Second-generation percutaneous bioprosthetic valve: A short-term study in sheep. J Vasc Surg, 2004,40(6): 1223-1227.
[16]Teebken OE, Puschmann C, Aper T, et al. Tissue-engineered bioprosthetic venous valve: a long-term study in sheep. Eur J Vasc Endovasc Surg, 2003, 25(4):305-312.
[17]Gulbins HE, Pritisanac A, Uhlig A, et al. Seeding of Human Endothelial Cells on Valve Containing Aortic Mini-Roots: Development of a Seeding Device and Procedure. Ann Thorac Surg, 2005, 79(6):2119-2126.
[18] HSU SH, Tsai IJ, Lin DJ, et aL The effect of dynamic culture conditions on endothelial cell seeding and retention on small diameter polyurethane vascular grafts. Med Eng Phys, 2005, 27(3):267-272.
[19] Abilez O, Benharash P, Mehrotra M, et aL A Novel Culture System Shows that Stem Cells Can be Grown in 3D and Under Physiologic Pulsatile Conditions for Tissue Engineering of Vascular Grafts. J Surg Res, 2006,132(2): 170-178.
[20]Buttafoco L, Engbers-Buijtenhuijs P, Poot AA, et al Physical characterization of vascular grafts cultured in a bioreactor. Biomaterials, 2006, 27(11):2380-2389.
[1]Pittenger MF,Mackay AM,Beck SC,et al.Multilineage potential of adult human mesenchymal stem cells.Science,1999,284(5411):143-147.
[2]Jiang Y,Vaessen B,Lenvik T,et al.Multipotent progenitor cells can be isolated from postnatal murine bone marrow,muscle,and brain.Exp Hematol,2002,30(8):896-904.
[3]Jiang Y,Jahagirdar BN,Reinhardt RL,et al.Pluripotency of mesenchymal stem cells derived from adult marrow.Nature,2002,418(6893):41-49.
[4]幕宁,高毅,唐力军.双阴性选择法纯化培养人骨髓多能成体祖细胞.第一军医大学学报,2005,25(5):535-537.
[5]陈黎红,程桦,闵军,等.小鼠骨髓多能成体祖细胞的分离、培养及体外诱导分化为胰岛素分泌细胞的研究.中国病理生理杂志,2005,21(9):1797-1801.
[6]Tolar J,O'shaughnessy MJ,Panoskaltsis-Mortari A,et al.Host factors that impact the biodistribution and persistence of multipotent adult progenitor cells.Blood,2006,107(10):4182-4188.
[7]Grove JE,Bruscia E,Krause DS.Plasticity of bone marrow-derived stem cells.Stem Cells,2004,22(4):487-500.
[8]Jin Y,Fuller L,Ciancio G,et al.Antigen presentation and immune regulatory capacity of immature and mature-enriched antigen presenting(dendritic) cells derived from human bone marrow.Hum Immunol,2004,65(2):93-103.
[9]Serafini M,Dylla SJ,Oki M,et al.Hematopoietic reconstitution by multipotent adult progenitor cells:precursors to long-term hematopoietic stem cells.J Exp Med,2007,204(1):129-139.
[10]Reyes M,Li S,Foraker J,et al.Donor origin of multipotent adult progenitor cells in radiation chimeras.Blood,2005,106(10):3646-3649.
[11]Muguruma Y,Reyes M,Nakamura Y,et al.In vivo and in vitro differentiation of myocytes from human bone marrow-derived multipotent progenitor cells.Exp Hematol,2003,31(12):1323-1230.
[12]Schwartz RE,Reyes M,Koodie L,et al.Multipotent adult progenitor cells from bone marrow differentiate into funetional hepatocyte-like cells.J Clin Invest,2002,109(10):1291-1302.
[13]Ross JJ,Hong Z,Willenbring B,et al.Cytokine-induced differentiation of multipotent adult progenitor cells into functional smooth muscle cells.J Clin Invest.2006,116(12):3139-3149.
[14]Prosper F,Stroncek D,Verfaillie CM.Verfaillie.Phenotypic and functional characterization of long-term culture-initiating cells present in peripheral blood progenitor collections of normal donors treated with granulocyte colony-stimulating factor.Blood,1996,88(6):2033-42.
[1] Hur J,Yoon C H,Kim H S,et al.Characterization of two types of endothelial progenitor cells and their different contributions to neovasculogenesis. Arterioscler Thromb Vasc Biol, 2004,24 (2): 288-293.
[2] Nishiwaki Y, Yoshida M, Iwaguro H, et al. Endothelial E-selectin potentiates neovascularization via endothelial progenitor cell-dependent and -independent mechanisms. Arterioscler Thromb Vasc Biol, 2007, 27(3):512-518.
[3] Asahara T, Masuda H, Takahashi T, et al. Bone marrow origin of endothelial progenitor cells responsible for postnatal vasculogenesis in physiological and pathological neovascularization. Circ Res, 1999, 85(3): 221-228.
[4] Hill JM, Zalos G, Halcox JP, et al. Circulating endothelial progenitor cells, vascular function, and cardiovascular risk. N Engl J Med, 2003, 348(7):593-600.
[5] Bellik L, Ledda F, Parenti A. Morphological and phenotypical characterization of human endothelial progenitor cells in an early stage of differentiation.FEBS LETTERS, 2005,579(12): 2731-2736.
[6] Matsumura G, Miyagawa-Tomita S, Shin'oka T,et al. First evidence that bone marrow cells contribute to the construction of tissue-engineered vascular autografts in vivo. Circulation, 2003,108(14): 1729-1734.
[7] Asahara T, Murohara T, Sullivan A, et al. Isolation of putative progenitor endothelial cells for angiogenesis. Science, 1997, 275(5302):964-967.
[8] Lin Y, Weisdorf D, Solovey A, et al. Origins of circulating endothelial cells and endothelial outgrowth from blood. J Clin Invest, 2000,105(1):71-77.
[9] Reyes M, Dudek A, Jahagirdar B, et al. Origin of endothelial progenitors in human postnatal bone marrow. J Clin Invest, 2002,109(3):337-346.
[10]Wassmann S, Werner N, Czech T, et al. Improvement of endothelial function by systemic transfusion of vascular progenitor cells.Circ Res, 2006,99(8):e74-83.
[11]Popa E R, Harmsen M C, Tio R A, et al. Circulating CD34~+ progenitor cells modulate host angiogenesis and inflammation in vivo. J Mol Cell Cardiol, 2006, 41(1):86-96.
[12]Pardanaud L, Eichmann A. Identification, emergence and mobilization of circulating endothelial cells or progenitors in the embryo. Development. 2006, 133(13):2527-2537.
[13]Sharpe E E 3rd, Teleron AA, Li B, et al. The origin and in vivo significance of murine and human culture-expanded endothelial progenitor cells. Am J Pathol, 2006, 168(5): 1710-1721.
[14]Butzal M, Loges S, Schweizer M. et al. Rapamycin inhibits proliferation and differentiation of human endothelial progenitor cells in vitro. Exp Cell Res, 2004, 300(1):65-71.
[15] Inoue T, Sata M, Hikichi Y, et al. Mobilization of CD34"positive bone marrow-derived cells after coronary stent implantation: impact on restenosis. Circulation, 2007, 115(5):553-561.
[16]Murayama T, Tepper OM, Silver M, et al. Determination of bone marrow-derived endothelial progenitor cell significance in angiogenic growth factor-induced neovascularization in vivo. Exp Hematol, 2002, 30(8): 967-972.
[1]Kasimir MT,Weigel G,Sharma J,et al.The decellularized porcine heart valve matrix in tissue engineering:platelet adhesion and activation.Thromb Haemost,2005, 94(3):562-567.
[2]Seung-Woo Cho,Sang HL,Il-Kwon Kim,et al.Small-Diameter Blood Vessels Engineered With Bone Marrow-Derived Cells.Ann Surg,2005,241(3):506-515.
[3]J.Andrew McKeel,Soma,S.R.Banik,et al.Human arteries engineered in vitro.EMBO,2003,4(6):633-638.
[4]VandeVondele S.Voros J.Hubbell JA.RGD-grafted poly-L-lysine-graft-(polyethylene glycol) copolymers block non-specific protein adsorption while promoting cell adhesion.Biotechnol Bioeng,2003,82(7):784-790.
[5]Kim TG.Park TG.Biomimicking extracellular matrix:cell adhesive RGD peptide modlfied electrospun poly(D,L-lactic-co-glycolic acid) nanofiber mesh.Tissue Eng,2006,12(2):221-233.
[6]Matsumura G,Sachiko MT,Toshiharu S,et al.First Evidence That Bone Marrow Cells Contribute to the Construction of Tissue-Engineered Vascular Autografts In Vivo.Circulation,2003,108(14):1729-1734.
[7]Kallenbach K,Leyh RG,Lefik E,et al.Guided tissue regeneration:porcine matrix does not transmit PERV.Biomaterials,2004,25(17):3613.
[8]Schmidt CE,Baier JM.Acellular vascular tissues:natural biomaterials for tissue repair and tissue engineering.Biomaterials,2000,21(22):2215-31.
[9]SHEN G,TSUNG HC,WU CHF,et al.Tissue engineering of blood vessels with endothelial cells differentiated from mouse embryonic stem cells.Cell research,2003,13(5):335-341.
[10]Kerdjoudj H,Boura C,Marchal L,et al.Decellularized umbilical artery treated with thin polyelectrolyte multilayer films:potential use in vascular engineering.Biomed Mater Eng,2006,16(4 Suppl):S123-129.
[11]刘太华,张炎,姜宗来.组织工程血管脱细胞支架主要组织相容性复合体Ⅰ抗原性的免疫组化观察.第二军医大学学报,2004,25(1):72-74.
[12]McFetridge PS,Abe K,Horrocks M,et al.Vascular tissue engineering:bioreactor design considerations for extended culture of primary human vascular smooth muscle cells.ASAIO J,2007,53(5):623-630.
[13]Shimizu K,Ito A,Arinobe M,et al.Effective cell-seeding technique using magnetite nanoparticles and magnetic force onto decellularized blood vessels for vascular tissue engineering.J Biosci Bioeng,2007,103(5):472-478.
[14]Lopez-Soler RI,Brennan MP,Goyal A,et al.Development of a mouse model for evaluation of small diameter vascular grafts. J Surg Res, 2007,139(1):1-6.
[1]de Borst GJ,Teijink JA,Patterson M,et al.A percutaneous approach to deep venous valve insufficiency with a new self-expanding venous frame valve.J Endovasc Ther,2003,10(2):341-349.
[2]Boudjemline Y,Bonnet D,Sidi D,et al.Ispercutaneous implantation of a bovine venous valve in the inferior vena cava a reliable technique to treat chronic venous insufficiency syndrome? Med Sci Monit,2004,10(3):BR61-66.
[3]Bry JD,Muto PA,O'Donnell TF,et al.The clinical and hemodynamic results after axillary-to-popliteal vein valve transplantation.J Vasc Surg,1995,21(1):110-119.
[4]Makarova NP,Hmelniker SM,Lurie F.Does surgical correction of the superficial femoral vein valve change the course of varicose disease? J Vasc Surg,2001,33(2):361-368.
[5]Kucher T,Brener B,Marak M,et al.Endovascular delivery of vein segments with valves versus direct anastomosis.J Endovasc Ther.2005,12(3):366-70.
[6]Vijayan V,Shukla N,Johnson JL,et al.Long-term reduction of medial and intimal thickening in porcine saphenous vein grafts with a polyglactin biodegradable external sheath.J Vasc Surg.2004,40(5):1011-9.
[7]Lichtenberg A,Tudorache I,Cebotari SP,et al.Testing of Tissue-Engineered Heart Valves Re-Endothelialized Under Simulated Physiological Conditions.Circulation.2006,114(1 Suppl):I559-565.
[8]Kasimir MT,Weigel G,Sharma J,et al.The decellularized porcine heart valve matrix in tissue engineering:platelet adhesion and activation.Thromb Haemost,2005,94(3):562-567.
[9]Kazunori Shiminu,Akira Ito,Manabu Arinobe,et al.Effective cell-seeding technique using magnetite nanoparticles and magnetic force onto decellularized blood vessels for vascular tissue engineering.J Bios &Bioe.2007,103(5):472-478.
[10]Sandip Sarkar,Thomas SR,George Hamilton,et al.Achieving the ideal properties for vascular bypass grafts using a tissue engineered approach:a review.Med Bio Eng Comput.2007,45(4):327-336.
[11]Pavcnik D, Barry T, Hans A, et al. Percutaneous bioprosthetic venous valve: A long-term study in sheep. J Vasc Surg, 2002,35(3):598-603.
[12]Teebken OE, Puschmann C, Aper T, et al. Tissue-engineered bioprosthetic venous valve: a long-term study in sheep. Eur J Vasc Endovasc Surg, 2003, 25(4):305-312.
[13]Matsumura G, Miyagawa-Tomita S, Shin'oka T,et al. First Evidence That Bone Marrow Cells Contribute to the Construction of Tissue-Engineered Vascular Autografts In Vivo. Circulation. 2003,108(14):1729-1734.
[14]Asahara T, Murohara T, Sullivan A, et al. Isolation of putative progenitor endothelial cells for angiogenesis. Science. 1997, 275(5302):964-967.
[15]Tolar J, O'shaughnessy MJ, Panoskaltsis-Mortari A, et al. Host factors that impact the biodistribution and persistence of multipotent adult progenitor cells. Blood, 2006, 107(10): 4182-4188.
[16] Jiang Y, Jahagirdar BN, Reinhardt RL, et al. Pluripotency of mesenchymal stem cells derived from adult marrow. Nature, 2002, 418(6893):41-49.
[17] Schwartz RE, Reyes M, Koodie L, et al. Multipotent adult progenitor cells from bone marrow differentiate into functional hepatocyte-like cells. J Clin Invest, 2002, 109(10):1291-1302.
[18]Muguruma Y, Reyes M, Nakamura Y, et al. In vivo and in vitro differentiation of myocytes from human bone marrow-derived multipotent progenitor cells.Exp Hematol, 2003, 31(12):1323-1230.
[19]Wassmann S, Werner N, Czech T, et al. Improvement of endothelial function by systemic transfusion of vascular progenitor cells. Circ Res. 2006, 99(8):e74-83.
[20]Hur J, Yoon CH, Kim HS, et al.Characterization of two types of endothelial progenitor cells and their different contributions to neovasculogenesis. Arterioscler Thromb Vasc Biol. 2004, 24(2): 288-293
[21] Awad O, Dedkov EI, Jiao C, et al. Differential healing activities of CD34+ and CD14+ endothelial cell progenitors. Arterioscler Thromb Vase Biol. 2006, 26(4):758-64.
[22]Abilez O, Benharash P, Mehrotra M, et al. A Novel Culture System Shows that Stem Cells Can be Grown in 3D and Under Physiologic Pulsatile Conditions for Tissue Engineering of Vascular Grafts. J Surg Res, 2006,132(2): 170-178.
[23] Buttafoco L, Engbers-Buijtenhuijs P, Poot AA, et al. Physical characterization of vascular grafts cultured in a bioreactor. Biomaterials, 2006, 27(11):2380-2389.
[1]Boudjemline Y,Bonnet D,Sidi D,et al.Ispercutaneous implantation of a bovine venous valve in the inferior vena cava a reliable technique to treat chronic venous insufficiency syndrome? Med Sci Monit,2004,10(3):BR61-66.
[2]Makarova NP,Hmelniker SM,Lurie F.Does surgical correction of the superficial femoral vein valve change the course of varicose disease? J Vasc Surg,2001,33(2):361-368.
[3]Pavcnik D,Barry T,Hans A,et al.Percutaneous bioprosthetic venous valve:A long-term study in sheep.J Vasc Surg,2002,35(3):598-603.
[4]Pavcnik D,Kaufman J,Uchida B,et al.Second-generation percutaneous bioprosthetic valve:A short-term study in sheep.J Vasc Surg,2004,40(6):1223-1227.
[5]de Borst GJ,Teijink JA,Patterson M,et al.A percutaneous approach to deep venous valve insufficiency with a new self-expanding venous frame valve.J Endovasc Ther,2003,10(2):341-349.
[6]Teebken OE,Puschmann C,Aper T,et al.Tissue-engineered bioprosthetic venous valve:a long-term study in sheep.Eur J Vasc Endovasc Surg,2003,25(4):305-312.
[7]Thorpe PE,Osse FJ,Correa LO.The valve-stent:development of a percutaneous prosthesis for treatment of valvular insufficiency.Presented at the 12th Annual meeting of the American Venous Forum.2000,2:3-6.
[8]Pavcnik D,Uchida BT,Timmermans H,et al.The square stent-based large vessel occluder:an experimental pilot study.J Vasc Interv Radiol.2001,12(11):1353-1355.
[9] Teebken OE, Pichlmaier AM, Haverich A. Cell seeded decellularised allogeneic matrix grafts and biodegradable polydioxanone-prostheses compared with arterial autografts in a porcine model. Eur J Vasc Endovasc Surg 2001, 22(2): 139-145.
[1]Makarova NP,Hmelniker SM,Lurie F.Does surgical correction of the superficial femoral vein valve change the course of varicose disease? J Vasc Surg,2001,33(2):361-368.
[2]Bry JD,Muto PA,O'Donnell TF,et al.The clinical and hemodynamic results after axillary-to-popliteal vein valve transplantation.J Vasc Surg,1995,21(1):110-119.
[3]Boudjemline Y,Bonnet D,Sidi D,et al.Ispercutaneous implantation of a bovine venous valve in the inferior vena cava a reliable technique to treat chronic venous insufficiency syndrome? Med Sci Monit,2004,10(3):BR61-66.
[4]Kasimir MT,Weigel G,Sharma J,et al.The decellularized porcine heart valve matrix in tissue engineering:platelet adhesion and activation.Thromb Haemost,2005,94(3):562-567.
[5]Pavcnik D,Barry T,Hans A,et al.Percutaneous bioprosthetic venous valve:A long-term study in sheep.J Vasc Surg,2002,35(3):598-603.
[6]Pavcnik D,Kaufman J,Uchida B,et al.Second-generation percutaneous bioprosthetic valve:A short-term study in sheep.J Vasc Surg,2004,40(6):1223-1227.
[7]de Borst GJ,Teijink JA,Patterson M,et al.A percutaneous approach to deep venous valve insufficiency with a new self-expanding venous frame valve.J Endovasc Ther,2003,10(2):341-349.
[8]刘太华,张炎,姜宗来,等.组织工程血管脱细胞支架主要组织相容性复合体Ⅰ抗原性的免疫组化观察.第二军医大学学报,2004,25(1):72-74.
[9]Cebotari S,Mertsching H,Kallenbach K,et al.Construction of autologous human heart valves based on an acellular allograft matrix.Circulation,2002,106(12 Suppl 1):I63-68.
[10]Teebken OE,Puschmann C,Aper T,et al.Tissue-engineered bioprosthetic venous valve:a long-term study in sheep.Eur J Vasc Endovasc Surg,2003,25(4):305-312.
[11]董念国,叶晓峰,史嘉伟,等.组织工程瓣膜天然支架去细胞方法的比较.中华实验外科杂志,2005,22(3):377.
[12]Schnell AM,Hoerstrup SP,Zund G,et al.Optimal cell source for cardiovascular tissue engineering:venous vs.aortic human myofibroblasts.Thorac Cardiovasc Surg,2001,49(4):221-225.
[13]He H,Shirota T,Yasui H,et al.Canine endothelial progenitor cell-lined hybrid vascular graft with nonthrombogenic potential.J Thorac Cardiovasc Surg,2003,126(2):455-464.
[14]Fadini GP,Agostini C,Avogaro A.Characterization of endothelial progenitor cells.Biochem Biophys Res Commun,2005,336(1):1-2.
[15]Amit M,Carpenter MK,Inokuma MS,et al.Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged period of culture.Dev Biol,2000,227(2):271-278.
[16]Abilez O,Benharash P,Mehrotra M,et al.A Novel Culture System Shows that Stem Cells Can be Grown in 3D and Under Physiologic Pulsatile Conditions for Tissue Engineering of Vascular Grafts.J Surg Res,2006,132(2):170-178.
[17]Buttafoco L,Engbers-Buijtenhuijs P,Poot AA,et al.Physical characterization of vascular grafts cultured in a bioreactor.Biomaterials,2006,27(11):2380-2389.
[18]Gulbins HE,Pritisanac A,Uhlig A,et al.Seeding of Human Endothelial Cells on Valve Containing Aortic Mini-Roots:Development of a Seeding Device and Procedure.Ann Thorac Surg,2005,79(6):2119-2126.
[19]HSU SH,Tsai IJ,Lin DJ,et al.The effect of dynamic culture conditions on endothelial cell seeding and retention on small diameter polyurethane vascular grafts.Med Eng Phys,2005,27(3):267-272.
[20]Hench LL,Polak JM.Third-generation biomedical materials.Science,2002,295(5557):1014-1017.
[21]Ye O,Zund G,Benedikt P,et al.Fibrin gel as a three dimensional matrix in cardiovascular tissue engineering.Eur J Cardiothorac Surg,2000,17(5):587-591.
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