长白山蕨类植物宏观形态及表观纹理结构研究
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摘要
陆静梅教授的课题组在长达19年的研究过程中,首次成功地研究了光学显微镜的暗差扫描摄影技术,为植纹鉴定创出一条新路,解决了尸体胃内容物极度腐烂时,不能进行封片处理的技术难题,为刑事侦查破案提供了准确、便捷的植纹光学显微镜的暗差三维扫描成像技术,获得了突破性的重要研究成果。
     本文采用了陆静梅教授的植纹专利技术,对采自长白山地区1700-2100m之间蕨类植物宏观形态及表观纹理结构进行了系统的分析,蕨类植物世界主要分布于热带地区,有约50属,600种。我国分布全国各地,有约27属,150种。通过光学显微镜的观察研究,将杉蔓石松(Lycopodium annotinum L.),卷柏(Selaginella tamariscina (Beauv.)Spr.),问荆(Equisetum arvense L.),蕨(P.aquilinum(L.)Kuhn),分株紫萁(Osmunda cinnamomea L.var.asiatica Fernald),猴腿蹄盖蕨(Athyrium multidentatum (Doeee)Ching)这六种代表性的蕨类植物作为主要的研究对象。实验结果显示上述六种植物的植纹特征分别为如下的几种类型:杉蔓石松Lycopodium annotinum L.的植纹(细胞壁C型);卷柏Selaginella tamariscina (Beauv.)Spr.的植纹(气孔在胞间隙缝中,保卫细胞半月形,细胞壁G型);问荆的植纹Equisetum arvense L.(细胞壁E型);分株紫萁Osmunda cinnamomea L.var.asiatica Fernald的植纹(细胞壁F型);蕨P.aquilinum(L.)Kuhn的植纹(细胞壁F型);猴腿蹄盖蕨Athyrium multidentatum (Doeee)Ching的植纹(细胞壁F型)。研究结果表明:结合蕨类植物的植纹类型以及表皮附属物类型可以对长白山地区植物进行有效的鉴别。本文讨论的几种植物都有一定的药效价值,这对以后药用植物的鉴定和分析具有一定的意义。
     植纹有很高的使用价值,可作为“植纹”标准比对样本,直接用于检验鉴定。这项研究可在刑事侦察中推广使用,以拓宽刑侦技术检验鉴定的范围。“植纹”鉴定研究为我国刑侦科学技术开辟了新领域,填补了国内、外空白。
In the several decades of research and study, Professor Lu Jingmei led a team to complete the study of plant print. It is the first time to use the dark division scanning technique successfully. it gives us a new way to study the plant print. It solves the problem of the extremely decomposed stomach contents can not be mounted. The plant print three-dimensional(3D) optical microscopy scan-imaging technology provides exactly and conveniently investigation clues for criminal detection. It has obtained the unprecedented development.
     In the present study, the leaf epidermises of Pteridophyta plants in 1700-2100m of Changbai Mountain were observed with optical microscopy using the Plant Print technology. There are about 50 genera, 600 species , mainly in the tropics. There are about 27 genera, 150 species in China and they distribute all over the country. We observed and studied about 6 species of ferns from Changbai Mountain, They are, respectively, Lycopodium annotinum L.; Selaginella tamariscina (Beauv.)Spr.; Equisetum arvense L.; P.aquilinum(L.)Kuhn; Osmunda cinnamomea L.var.asiatica Fernald; Athyrium multidentatum (Doeee)Ching. The results indicated that the plant print of Lycopodium annotinum L.cell wall is type C; The plant print of Selaginella tamariscina (Beauv.)Spr. cell wall is type G; The plant print of Equisetum arvense L. cell wall is type E; The plant print of Osmunda cinnamomea L.var.asiatica Fernald.cell wall is type F; The plant print of P.aquilinum(L.)Kuhn .Cell wall is type F; The plant print of Athyrium multidentatu(Doeee)Ching. cell wall is type F . The results indicated that, Pteridophyte in Changbai Mountain could be effectively identified according to the types of Plant Print and appendages of the leaf epidermises.
     The results show that the plant print has practical and high value, which could be use as the standard comparisons. It is directly used for examination appraisal. The technology can be used more widely in the area of criminal detection. The study of Plant Print apparent Structural opens new realms of criminal detection. The technology is the first at home and abroad to fill the gaps in the field.
引文
[1]陆静梅.植物表观结构植纹鉴定[M].北京:科学出版社,2010.11-42.(待出版)
    [2]陆静梅.刑事侦查植物宏观和微观植纹破案方法[P].中国专利,2003101158071.2006-03-01.
    [3]周繇.长白山国家级自然保护区观赏植物资源及其多样性[J].东北林业大学学报,2004,32(6):45-50.
    [4]周繇,刘利,张明杰等.长白山国家级自然保护区药用植物资源及其多样性研究[J].林业科学,2005,14(6):57-64.
    [5]傅立国,等.中国高等植物(第三卷)[M].青岛:青岛出版社,2000.404.
    [6]中国科学院中国植物志编辑委员会.中国植物志(27卷)[M].北京:科学出版社,1979.113-115,174.
    [7]矢志诚,等.中国草地重要有毒植物[M].北京:中国农业出版社,1997.208-213.
    [8]中科院植物志编委会.中国植物志:第34卷第1分册[M].北京:科学出版社, 1984: 161 -220
    [9]中科院植物志编委会.中国植物志:第16卷第1分册[M].北京:科学出版社, 1984: 131
    [10]中科院植物志编委会.中国植物志:第55卷第3分册[M].北京:科学出版社, 1984: 222.
    [11]国家药典委员会.中华人民共和国药典[M].北京:化学工业出版社,2005:102.
    [12]阴健,郭力云.中药现代研究与临床应用I[M].北京:学苑出版社,1994:329.
    [13]中科院植物志编委会.中国植物志:第65卷第2分册[M].北京:科学出版社, 1984: 194-198.
    [14]中科院植物志编委会.中国植物志:第42卷第2分册[M].北京:科学出版社, 1984: 109.
    [15]中科院植物志编委会.中国植物志:第13卷第2分册[M].北京:科学出版社, 1984: 102.
    [16]陆静梅,等.气孔的发育及其类型.生物学教学.1992年第1期
    [17]周瑛.气孔复体的观察.内部资料.1976.
    [18]陆静梅,等.一种新型的植物细胞结晶.武汉植物研究.1991年第9卷,第3期,p206-208。
    [19]接志政.表皮组织细胞学在消化残渣鉴定中的应用.刑侦技术.1989年,,第39期,p2-26。
    [20]植纹与刑事侦查,1989年广江美之助(日本),杨济春译,刑侦技术,第39期,p43-49。
    [21]刘慎愕.等.东北植物检索表.科学出版社.1959年.
    [22]陆时万、吴国芳.植物学(上、下册).人民教育出版社.1979.
    [23] David L.Dilcher. Approaches to the Identification of Angiosperm Leaf Remains, Botanical Review Vol.40,p1-157. 1974.
    [24] Katherine Esau, John Wiley and Sons Inc. Plant Anatomy. New York. 1965.
    [25]郭景元.实用法医学.上海科技出版社.1980.
    [26] Wang W-C (王文采). 1979. Aconitum L. In: Flora Reipublicae Popularis Sinicae (中国植物志). Beijing:Science Press. 27: 113-362.
    [27] Luo Y, Yang Q-E. 2005. Taxonomic revision of Aconitum (Ranunculaceae) from Sichuan, China. Acta Phytotaxonomica Sinica 43: 289–386.
    [28] Yang Q-E. Systematic studies on the genus Aconitum L. (Ranunculaceae) from Yunnan. Ph.D. Dissertation. Kunming: Kunming Institute of Botany, the Chinese Academy of Sciences. 1990.
    [29] Yang Q-E. Taxonomic notes on some species of Aconitum L. (Ranunculaceae) from Yunnan, China.Acta Phytotaxonomica Sinica 37: 545–590. 1999.
    [30] Yang Q-E. Cytology of 12 species in Aconitum L. and of 18 species in Delphinium L. of the tribe Delphineae (Ranunculaceae) from China. Acta Phytotaxonomica Sinica 39: 502–514. 2001.
    [31] Yang Q-E, Gu Z-J, Wu Z-Y, Hong D-Y. 1993a. A karyomorphological study in Aconitum subgen. Aconitum (Ranunculaceae) from Yunnan, China. Cathaya 5: 89–114.
    [32] Yang Q-E, Wang X-Q, Hong D-Y. A karyotype study of species of Aconitum L. from China. Journal of Plant Research and Environment 2: 33–38. 1993b.
    [33] Kadota Y. Aconitum L. In: Wu Z-Y, Raven P H eds. Flora of China. Beijing: Science Press; St. Louis: Missouri Botanical Garden Press. 6: 149–222. 2001.
    [34]王光野.盐生植物耐盐结构特性及植物晶体生物学特征与功能研究[D]:[博士学位论文].长春:东北师范大学,2009.
    [35] Tamura M. Aconitum L. In: Hiepko P ed. Die Natürlichen Pflanzenfamilien, zweite Auflage. Berlin: Dunker & Humblot. 17a (4): 274–291. 1995.
    [36] Luo Y. Taxonomic revision of Aconitum L. (Ranunculaceae) from Sichuan with a study on phylogeny of this genus based on molecular evidence. Ph.D. Dissertation. Beijing: Institute of Botany, the Chinese Academy of Sciences. 2003.
    [37] Yang Q E, Gu Z J, Wu Z Y, Kondo K. 1989. A karyomorphological study of some Yunnan species of Aconitum L. (Ranunculaceae). La Kromosomo 55–56: 1838–1860.
    [38] Yang Q-E, Gu Z-J, Wu Z-Y. A karyomorphological study in Aconitum subgen. Lycoctononum (Ranunculaceae) from Yunnan. Acta Botanica. Yunnanica 16: 61–74. 1994.
    [39] Xiao P-G, Wang L-W, Tong Y-Y. Correlation among the root morphology, phylogeny, main constituent and toxicity of 27 Chinese aconites. Chinese Journal of Pharmceutical Analysis 3: 276–280. 1983.
    [40] Xiao P-G, Chen D-H, Song W-L Phytochemical evidence . related to the phylogeny of Aconitum gymnandrum Maxim. Acta Phytotaxonomica Sinica 22: 53–56. 1984.
    [41] Hao X-J, Yang C-R, Chen S-Y, Zhou J. The chemotaxonomy of Chinese species of the genus Aconitum L. (Ranunculaceae). Acta Phytotaxonomica Sinica 23: 321–325. 1985.
    [42] Xiao Pei-Gen, Wang Feng-Peng, Gao Feng, Yan Lu-Ping, Chen Dong-Lin, Liu Yong. A pharmacophylogenetic study of Aconitum L. (Ranunculaceae) from China. Acta Phytotaxonomica Sinica, 44 (1): 1–46. 2006.
    [43] Lu Jingmei. Chinese Criminal activity Detection Plant Macroscopic And Microscopic Plant Print to Solve a Case New Technology: China, patent number:2003101158071 [P]. 2006-03-01
    [44] Qi Junsheng, Xu Huibi, Zhou Jingyan, Lu Xiaohua, Yang Xiangliang, Guan Jinghua, Factor analysis and cluster analysis of trace elements in some Chinese medicinal herbs. Chinese Journal of Analytical Chemistry, 26(11):1309-1314. 1998.
    [45] Conover M. Epidermal patterns of the reticulate-veined Liliiflorae and their parallel-veined allies. Bot J Linn Soc , 1991, 107 :295~312
    [46] Dong J-Y, Li L. The norditerpenoid alkaloids from the root of Aconitum nagarum Stapf var. lasiandrum W. T. Wang in China. Journal of Plant Resources and Environment 9:1–3. 2000.
    [47]谷安根,陆静梅,王立军.维管植物演化形态学[M].长春:吉林科学技术出版社,1993.120-127.
    [48]叶创兴,朱念德,廖文波,等.植物学[M].北京:高等教育出版社,2007.55-59.
    [49]李正理,李荣敖.我国甘肃九种旱生植物同化枝的解剖观察[J].Journal of Integrative Plant Biology,1981(3):181-185.