母猪sFBP基因的多态性及其与产仔数的关联分析
摘要
本研究利用3个猪品种／群体(大白猪A猪群,大白猪B猪群和湖南黑猪)共300多头猪为研究对象。采用PCR-SSCP结合PCR-Taq I RFLP确定并分析了sFBP基因中一个错义SNP (T388A)在所研究群体中的分布,并对品种内基因型频率进行了卡方适合性检验。鉴于sFBP在胎儿发育及母胎间叶酸转运过程中的关键性作用；同时,生物信息学分析表明T388A所导致的氨基酸置换正处于该蛋白质的保守区,本研究关注的焦点是sFBP基因T388A是否与母猪窝产仔数性状间存在显著性关联,及其遗传效应大小。主要结果如下：
1 sFBP基因T388A在3个品种中的基因频率和基因型频率
在3个群体中都呈现T等位基因为优势等位基因,并且AA纯合子个体稀少,在大白猪B猪群中没有发现AA纯合子母猪。在湖南黑猪、大白猪A群和大白猪B群中,等位基因A的频率分别为0.256、0.164和0.05。进行群体基因型频率x2适合性检验,结果表明在3个群体中,T388A都处于Hardy-Weinberg平衡状态。
2 sFBP基因T388A对产仔性状的影响
本研究基于大白猪A群探讨了sFBP基因对死胎、木乃伊胎和畸形胎的影响。不同基因型母猪在畸形胎指标上存在显著性差异,AA基因型母猪对比TT基因型和TA基因型有显著性差异,TT基因型和TA基因型母猪比AA基因型的畸形胎分别低0.404头和0.397头／窝次(P<0.05)。在木乃伊胎和死胎上,三种基因型间的差异不显著。
分析sFBP基因T388A对大白猪A群中母猪初产和经产窝产仔数性状的影响,或对大白猪A群与B群的合并群的经产窝产仔数性状的影响。结果表明,sFBP基因在大白猪TNB和NBA上具有中等大小的加性遗传效应(0.3-0.4头仔猪／窝次),同时显性遗传效应明显(0.6-0.9头仔猪／窝次)。因此,三种基因型大白猪母猪在TNB和NBA整体上都呈现TA>TT>AA,但是差异未达到统计显著水平。在湖南黑猪,研究sFBP基因T388A对母猪经产表现的影响,各基因型母猪在TNB上的相互关系表现为表现为TT>TA>AA,在NBA上的表现为AA>TT>TA,但以上差异都不显著。
本研究结果表明：sFBP基因T388A在大白猪中,在畸形胎方面,A等位基因对产畸形胎有显著性影响；各个群体组合中T388A的T等位基因显现出一定的高产仔性能的趋势。
Three swine herds consisted of more than 300 sampled sows (the A herd of Large White, the B herd of Large White and the herd of Hunan Black Pig) were employed in this study. a PCR-SSCP protocol was applied to genotype the predictive T388A in sFBP gene which affirmed by an error prone PCR-RFLP, and statistic its population distribution condition for herds concerned, and analyse genotype frequencies within breeds withχ2 test. Because of the key role in the fetal development and in maternal-fetal folate transport process of the sFBP; also, bioinformatics analysis showed that the T388A resulted amino acid transformation is in a conservative region of the protein, the focus of this study is the association between the sFBP gene T388A and the litter size traits of sows, and the genetic effect. The main results are as follows:
1 The gene frequencies and genotype frequencies of sFBP gene in three breeds
In the three breeds, T allele was the dominant allele, and AA is rare, in Large White pigs(B herd) found no AA homozygous sow. In Hunan Black pigs, Large White pigs(A herd) and Large White pigs(B herd), the allele frequency of A is 0.256,0.164 and 0.05. Analysis the genotype frequencies of groups withχ2 test, the results indicate that T388A is in Hardy-Weinberg equilibrium in three groups.
2 The effect of sFBP gene T388A on Litter Size
This study based on Large White(A herd), research the effect of sFBP gene on deformity, mummy and death fetus. The different genotype sows have significant difference on deformity fetus, AA genotype sows contrast with TA genotype sows and TT genotype sows, the deformity fetus are significant differences. TT and TA genotype sows are less than the AA genotype genotype sows with 0.404 and 0.397 on deformity fetus (P<0.05). On mummy and death fetus, the three genotypes was no significant difference. Analysis the effect of sFBP gene T388A on first litter size and all litter size in large white pigs(A herd), or the effect of sFBP gene T388A on all litter size in the combination group (A herd+B herd). The results show that, sFBP gene have a medium-sized additive genetic effects on TNB and NBA in Large White sows(0.3-0.4 piglets/litter), and the dominant genetic effects is significant (0.6-0.9 piglets/litter). Therefore, the three genotypes Large White sows are presented TA> TT> AA as a whole on TNB and NBA, but the difference was not statistically significant. In Hunan Black sows, research the effect of sFBP gene T388A on the performance of all litter size, the of all litter size is TT> TA> AA on TNB, on the NBA is AA> TT> TA, the above performance are no significantly differece.
The results show that: sFBP gene T388A in Large White sows, on deformity fetus, A allele is significantly affected on production of deformity fetus; although, In all groups, the results obtained that the T388A of T allele is beneficial for the trend of high litter size.
1 sFBP基因T388A在3个品种中的基因频率和基因型频率
在3个群体中都呈现T等位基因为优势等位基因,并且AA纯合子个体稀少,在大白猪B猪群中没有发现AA纯合子母猪。在湖南黑猪、大白猪A群和大白猪B群中,等位基因A的频率分别为0.256、0.164和0.05。进行群体基因型频率x2适合性检验,结果表明在3个群体中,T388A都处于Hardy-Weinberg平衡状态。
2 sFBP基因T388A对产仔性状的影响
本研究基于大白猪A群探讨了sFBP基因对死胎、木乃伊胎和畸形胎的影响。不同基因型母猪在畸形胎指标上存在显著性差异,AA基因型母猪对比TT基因型和TA基因型有显著性差异,TT基因型和TA基因型母猪比AA基因型的畸形胎分别低0.404头和0.397头／窝次(P<0.05)。在木乃伊胎和死胎上,三种基因型间的差异不显著。
分析sFBP基因T388A对大白猪A群中母猪初产和经产窝产仔数性状的影响,或对大白猪A群与B群的合并群的经产窝产仔数性状的影响。结果表明,sFBP基因在大白猪TNB和NBA上具有中等大小的加性遗传效应(0.3-0.4头仔猪／窝次),同时显性遗传效应明显(0.6-0.9头仔猪／窝次)。因此,三种基因型大白猪母猪在TNB和NBA整体上都呈现TA>TT>AA,但是差异未达到统计显著水平。在湖南黑猪,研究sFBP基因T388A对母猪经产表现的影响,各基因型母猪在TNB上的相互关系表现为表现为TT>TA>AA,在NBA上的表现为AA>TT>TA,但以上差异都不显著。
本研究结果表明：sFBP基因T388A在大白猪中,在畸形胎方面,A等位基因对产畸形胎有显著性影响；各个群体组合中T388A的T等位基因显现出一定的高产仔性能的趋势。
Three swine herds consisted of more than 300 sampled sows (the A herd of Large White, the B herd of Large White and the herd of Hunan Black Pig) were employed in this study. a PCR-SSCP protocol was applied to genotype the predictive T388A in sFBP gene which affirmed by an error prone PCR-RFLP, and statistic its population distribution condition for herds concerned, and analyse genotype frequencies within breeds withχ2 test. Because of the key role in the fetal development and in maternal-fetal folate transport process of the sFBP; also, bioinformatics analysis showed that the T388A resulted amino acid transformation is in a conservative region of the protein, the focus of this study is the association between the sFBP gene T388A and the litter size traits of sows, and the genetic effect. The main results are as follows:
1 The gene frequencies and genotype frequencies of sFBP gene in three breeds
In the three breeds, T allele was the dominant allele, and AA is rare, in Large White pigs(B herd) found no AA homozygous sow. In Hunan Black pigs, Large White pigs(A herd) and Large White pigs(B herd), the allele frequency of A is 0.256,0.164 and 0.05. Analysis the genotype frequencies of groups withχ2 test, the results indicate that T388A is in Hardy-Weinberg equilibrium in three groups.
2 The effect of sFBP gene T388A on Litter Size
This study based on Large White(A herd), research the effect of sFBP gene on deformity, mummy and death fetus. The different genotype sows have significant difference on deformity fetus, AA genotype sows contrast with TA genotype sows and TT genotype sows, the deformity fetus are significant differences. TT and TA genotype sows are less than the AA genotype genotype sows with 0.404 and 0.397 on deformity fetus (P<0.05). On mummy and death fetus, the three genotypes was no significant difference. Analysis the effect of sFBP gene T388A on first litter size and all litter size in large white pigs(A herd), or the effect of sFBP gene T388A on all litter size in the combination group (A herd+B herd). The results show that, sFBP gene have a medium-sized additive genetic effects on TNB and NBA in Large White sows(0.3-0.4 piglets/litter), and the dominant genetic effects is significant (0.6-0.9 piglets/litter). Therefore, the three genotypes Large White sows are presented TA> TT> AA as a whole on TNB and NBA, but the difference was not statistically significant. In Hunan Black sows, research the effect of sFBP gene T388A on the performance of all litter size, the of all litter size is TT> TA> AA on TNB, on the NBA is AA> TT> TA, the above performance are no significantly differece.
The results show that: sFBP gene T388A in Large White sows, on deformity fetus, A allele is significantly affected on production of deformity fetus; although, In all groups, the results obtained that the T388A of T allele is beneficial for the trend of high litter size.
引文
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