响应面法优化扁桃斑鸠菊黄酮提取及抗氧化活性研究
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摘要
为探讨提取扁桃斑鸠菊(Vernonia amygdalina Del.)黄酮及自由基的最佳提取工艺,以扁桃斑鸠菊的叶片为试材,运用单因素和响应面试验相结合的方法,通过超声波辅助对其总黄酮的提取进行优化,并对其黄酮含量进行测定与抗氧化活性分析。研究结果表明:扁桃斑鸠菊黄酮最佳提取条件为超声波功率409.6 W,超声频率35 kHz,乙醇浓度100%,料液比为1∶48.5 g/mL,提取温度61.9℃,提取时间20 min,黄酮得率为5.7%。当扁桃斑鸠菊黄酮浓度为2 mg/mL时,其对·OH、DPPH·和O_2~-·的最大清除率分别为54.7%、79.2%和32%,并具有很好的还原能力。通过热溶剂浸提超声波辅助提取法提取扁桃斑鸠菊中的黄酮类化合物,所用时间短,要求温度低,提取率好,方法简单易行,具有明显的优势。
In order to explore the optimal extraction process of flavonoids and free radicals from Vernonia amygdalina Del.,the leaves of Vernonia amygdalina Del. were used as test materials. The combination of single factor and response surface test was used to optimize the extraction of total flavonoids by ultrasonic assist,and the flavonoid content was determined and the antioxidant activity was analyzed. The results showed that the optimal extraction conditions of Vernonia amygdalina Del. flavonoids were that ultrasonic power was 409.6 W,ultrasonic frequency was 35 kHz,ethanol concentration was 100%,solid-liquid ratio was 1∶48.5 g/mL,extraction temperature was 61.9℃,extraction time was 20 min,and the flavonoid yield was 5.7%. When the concentration of flavonoids was 2 mg/mL,the maximum clearance rates of ·OH,DPPH· and O_2~-· were 54.7%,79.2% and 32%,respectively,they had good reducing ability. The flavonoids in Vernonia amygdalina Del. were extracted by ultrasonic assisted extraction with hot solvent extraction. The extraction time was short,the required temperature was low,and the extraction rate was good. This method was simple and easy to operate,which had obvious advantages.
In order to explore the optimal extraction process of flavonoids and free radicals from Vernonia amygdalina Del.,the leaves of Vernonia amygdalina Del. were used as test materials. The combination of single factor and response surface test was used to optimize the extraction of total flavonoids by ultrasonic assist,and the flavonoid content was determined and the antioxidant activity was analyzed. The results showed that the optimal extraction conditions of Vernonia amygdalina Del. flavonoids were that ultrasonic power was 409.6 W,ultrasonic frequency was 35 kHz,ethanol concentration was 100%,solid-liquid ratio was 1∶48.5 g/mL,extraction temperature was 61.9℃,extraction time was 20 min,and the flavonoid yield was 5.7%. When the concentration of flavonoids was 2 mg/mL,the maximum clearance rates of ·OH,DPPH· and O_2~-· were 54.7%,79.2% and 32%,respectively,they had good reducing ability. The flavonoids in Vernonia amygdalina Del. were extracted by ultrasonic assisted extraction with hot solvent extraction. The extraction time was short,the required temperature was low,and the extraction rate was good. This method was simple and easy to operate,which had obvious advantages.
引文
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[10]胡旭娇,刘彩霞,杨仙,等.miR-21在山核桃叶总黄酮抑制人脐静脉内皮细胞增殖中的作用研究[J].浙江中医药大学学报,2014,38(5):613-616,640.
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[17]史艳财,邹蓉,韦记青,等.黄花倒水莲总黄酮提取工艺研究[J].北方园艺,2014(12):134-137.
[18]赵娟娟.分心木黄酮超声-微波协同提取及抗氧化性研究[J].食品研究与开发,2018,39(18):70-76.
[19]许静雅,苏小军,李清明,等.响应面法优化淮山中总黄酮提取工艺的研究[J].中国酿造,2016,35(5):115-118.
[20]李云龙,李红梅,胡俊君,等.响应面法优化苦荞酒糟黄酮提取工艺的研究[J].中国酿造,2013,32(7):38-42.
[21]邓梦琴,何夏怡,何慕怡,等.响应面法优化菠萝蜜果皮黄酮提取工艺[J].食品工业科技,2016,37(5):222-227.
[22]焦文静,林洁荣,叶健军,等.响应面分析法优化紫象草花青素提取工艺[J].黑龙江畜牧兽医:科技版,2015(19):11-15,20,294.
[23]蔡碧琼.稻壳黄酮类化合物的提取、精制及抗氧化活性研究[D].福州:福建师范大学,2008.
[24]朱会霞.覆盆子黄酮抗氧化活性研究[J].现代食品科技,2012,28(10):1302-1305.
[25]吴玉兰.金樱子总黄酮对氧化损伤HUVEC保护作用的研究[D].衡阳:南华大学,2012.
[26]高行恩,王洪新.不同提取方法对山药多糖含量及其体外抗氧化活性的影响[J].食品与发酵工业,2015,41(7):256-262.
[2]SANI A A,ALEMIKA E T,ABDULRAHEEM R O,et al.A study review of documented phytochemistruy of Vernonia amygdalina(family asteraceae)as the basis for pharmacologic activity of plant extract[J].Journal of Natural Sciences Research,2012,2(7):1-8.
[3]JISAKA M,KAWANAKA M,SUGIYAMA H,et al.Antischistosomal activities of sesquiterpene lactones and steroid glucosides from Vernonia amygdalina,possibly used by wild chimpanzees against parasite-related diseases[J].Bioscience Biotechnology Biochemistry,1992,56(5):845-846.
[4]OBASEIKI-EBOR E E,ODUKOYA K,TELIKEPALLIH,et al.Antimutagenic activity of extracts of leaves of four common edible vegetable plants in Nigeria(west Africa)[J].Mutation Research Letters,1993,302(2):109-117.
[5]KUPCHAN S M,HEMINGWAY R J,KARIM A,et al.Tumor inhibitors.XLVII.Vernodalin and vernomygdin,two new cytotoxic sesquiterpene lactones from Vernonia amygdalina Del.[J].Journal of Organic Chemistry,1969,34(12):3908-3911.
[6]TADESSE A,GEBRE-HIWOT A,ASRES K,et al.The in vitro activity of Vernonia amygdalina on Leishmania aethiopica[J].Ethiopian Medical Journal,1993,31(3):183-189.
[7]闫鑫,李青旺,李健.蕨菜总黄酮对宫颈癌作用的研究[J].黑龙江畜牧兽医:科技版,2009(21):95-97.
[8]BUNTRU A,ROTH A,NYFFENEGGER-JANN N J,et al.HemITAM signaling by CEACAM3,a human granulocyte receptor recognizing bacterial pathogens[J].Archives of Biochemistry and Biophysics,2012,524(1):77-83.
[9]木合布力·阿布力孜,王永波,徐方野,等.新疆光果甘草黄酮对人肝癌Bel-7402细胞增殖的抑制活性研究[J].新疆医科大学学报,2013,36(12):1744-1748.
[10]胡旭娇,刘彩霞,杨仙,等.miR-21在山核桃叶总黄酮抑制人脐静脉内皮细胞增殖中的作用研究[J].浙江中医药大学学报,2014,38(5):613-616,640.
[11]孟庆华,于晓霞,张海凤,等.天然黄酮类化合物清除自由基机理及其应用进展[J].云南民族大学学报:自然科学版,2012,21(2):79-83.
[12]郭志芳,马川兰,贾娟.蜂胶中黄酮类化合物清除自由基性能研究[J].食品研究与开发,2014,35(7):17-19.
[13]江燕,林翠梧,柴桦,等.扁桃斑鸠菊挥发性化学成分的顶空固相微萃取-气相色谱-质谱法分析[J].时珍国医国药,2010,21(11):2884-2886.
[14]陆阳.从扁桃斑鸠菊中分得两种新皂甙[J].国外医药:植物药分册,1996,11(3):125-126.
[15]王华宇,陈乃明,杨利平,等.药用植物扁桃斑鸠菊的离体快速繁殖技术研究[J].湖北农业科学,2016,55(19):5087-5090.
[16]柳建军,许立松,刘锡葵.野生食用蔬菜甜菜树的抗氧化活性研究[J].食品科学,2008,29(8):125-127.
[17]史艳财,邹蓉,韦记青,等.黄花倒水莲总黄酮提取工艺研究[J].北方园艺,2014(12):134-137.
[18]赵娟娟.分心木黄酮超声-微波协同提取及抗氧化性研究[J].食品研究与开发,2018,39(18):70-76.
[19]许静雅,苏小军,李清明,等.响应面法优化淮山中总黄酮提取工艺的研究[J].中国酿造,2016,35(5):115-118.
[20]李云龙,李红梅,胡俊君,等.响应面法优化苦荞酒糟黄酮提取工艺的研究[J].中国酿造,2013,32(7):38-42.
[21]邓梦琴,何夏怡,何慕怡,等.响应面法优化菠萝蜜果皮黄酮提取工艺[J].食品工业科技,2016,37(5):222-227.
[22]焦文静,林洁荣,叶健军,等.响应面分析法优化紫象草花青素提取工艺[J].黑龙江畜牧兽医:科技版,2015(19):11-15,20,294.
[23]蔡碧琼.稻壳黄酮类化合物的提取、精制及抗氧化活性研究[D].福州:福建师范大学,2008.
[24]朱会霞.覆盆子黄酮抗氧化活性研究[J].现代食品科技,2012,28(10):1302-1305.
[25]吴玉兰.金樱子总黄酮对氧化损伤HUVEC保护作用的研究[D].衡阳:南华大学,2012.
[26]高行恩,王洪新.不同提取方法对山药多糖含量及其体外抗氧化活性的影响[J].食品与发酵工业,2015,41(7):256-262.
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