香蕉花蕾注射螺虫乙酯和吡虫啉对黄胸蓟马的防效及药剂在果实中的残留
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摘要
黄胸蓟马是中国香蕉上重要害虫,生产上对该虫仍缺乏较为有效的防治手段。本研究采用香蕉花蕾注射法施用22.4%螺虫乙酯悬浮剂(SC)和70%吡虫啉水分散粒剂(WG),研究了其对黄胸蓟马的防效,并采用超高效液相色谱-串联质谱法(UPLC-MS/MS)分析了药剂在香蕉果实中的残留。药效试验表明:于香蕉现蕾初期,22.4%螺虫乙酯SC和70%吡虫啉WG分别按有效成分0.12和0.18 g/株的剂量注射施药1次,对黄胸蓟马的防效分别为89%和86%。残留试验表明:花蕾注射施药后,螺虫乙酯和吡虫啉在香蕉果实中的半衰期分别为9.2和6.5 d,且在施药后95 d的香蕉成熟果实中未检测到其残留。本研究表明,香蕉花蕾注射螺虫乙酯与吡虫啉对黄胸蓟马具有良好的防治效果与安全性,可推荐在香蕉园轮换使用。
Thrips hawaiiensis(Morgan)(Thysanoptera: Thripidae) is an important pest on banana in China. However, there is no effective control method for this insect. In this study, the control efficiencies of 22.4% spirotetramat suspension concentrate(SC) and 70% imidacloprid water dispersible granule(WG) aganist T. hawaiiensis by banana flower injection method were studyed. And a method using ultra high performance liquid chromatography coupled with mass spectrometry(UPLC-MS/MS)was employed in the determination of spirotetramat and imidacloprid residues in fruit after banana flower injection. The efficacy trials showed that 22.4% spirotetramat SC and 70% imidacloprid WG were applied once at 0.12 and 0.18 g a.i. per plant in initial budding of banana by flower injection, the control efficacy on T. hawaiiensis were 89% and 86%, respectively. The residue detection results showed that the residue half-lives of spirotetramat and imidacloprid in banana fruit were 9.2 and 6.5 days, respectively.And 95 days after applied, their residues were not detected in the banana mature fruit. This study showed that banana flower injection of spirotetramat and imidacloprid had a good control efficacy on T. hawaiiensis and safety used in banana field, and it could be recommended to alternate use in banana orchards.
Thrips hawaiiensis(Morgan)(Thysanoptera: Thripidae) is an important pest on banana in China. However, there is no effective control method for this insect. In this study, the control efficiencies of 22.4% spirotetramat suspension concentrate(SC) and 70% imidacloprid water dispersible granule(WG) aganist T. hawaiiensis by banana flower injection method were studyed. And a method using ultra high performance liquid chromatography coupled with mass spectrometry(UPLC-MS/MS)was employed in the determination of spirotetramat and imidacloprid residues in fruit after banana flower injection. The efficacy trials showed that 22.4% spirotetramat SC and 70% imidacloprid WG were applied once at 0.12 and 0.18 g a.i. per plant in initial budding of banana by flower injection, the control efficacy on T. hawaiiensis were 89% and 86%, respectively. The residue detection results showed that the residue half-lives of spirotetramat and imidacloprid in banana fruit were 9.2 and 6.5 days, respectively.And 95 days after applied, their residues were not detected in the banana mature fruit. This study showed that banana flower injection of spirotetramat and imidacloprid had a good control efficacy on T. hawaiiensis and safety used in banana field, and it could be recommended to alternate use in banana orchards.
引文
[1]GOLDARAZENA A.First record of Thrips hawaiiensis(Morgan,1913)(Thysanoptera:Thripidae),an Asian pest thrips in Spain[J].EPPO Bull,2011,41(2):170-173.
[2]MURAI T.Development and reproductive capacity of Thrips hawaiiensis(Thysanoptera:Thripidae)and its potential as a major pest[J].Bull Entomol Res,2001,91(3):193-198.
[3]付步礼,李强,夏西亚,等.对乙基多杀菌素中度抗性降低黄胸蓟马的适合度[J].昆虫学报,2017,60(2):180-188.FU B L,LI Q,XIA X Y,et al.Moderate resistance to spinetoram reduces the fitness of Thrips hawaiiensis(Thysanoptera:Thripidae)[J].Acta Entomologica Sinica,2017,60(2):180-188.
[4]付步礼,唐良德,邱海燕,等.黄胸蓟马高效低毒防治新型药剂的筛选[J].果树学报,2016,33(4):473-481.FU B L,TANG L D,QIU H Y,et al.Screening of high effect and low toxicity insecticides for controlling Thrips hawaiiensis(Morgan)[J].JFruit Sci,2016,33(4):473-481.
[5]李强,付步礼,夏西亚,等.溴氰虫酰胺对香蕉田间黄胸蓟马的药效及其残留规律[J].农药学学报,2017,19(6):723-728.LI Q,FU B L,XIA X Y,et al.Efficiency of cyantraniliprole on Thrips hawaiiensis and its residue behaviour in banana field[J].Chin JPestic Sci,2017,19(6):723-728.
[6]MEYERDIRK D E,FRENCH J V,HART W G.Citrus mealybug:effect of pesticide residues on adults of the natural enemy complex[J].J Econ Entomol,1979,72(6):893-895.
[7]KOHJIMOTO T,NAGAOKA H,TAKAGI Y,et al.Side-effects of pesticide residues on strawberry leaves against natural enemies[J].Annu Rep Kanto-Tosan Plant Prot Soc,2004,51:145-149.
[8]KUMAR A R,RAHMAN V J,KUMAR D V,et al.Utilization of the bacterium,Pseudomonas putida as a potential biocontrol agent against red spider mite,Oligonychus coffeae(Acari:Tetranychidae)infesting tea[J].J Plantat Crops,2011,39(1):236-238.
[9]王慧瑜,赵玉安,贾耀军.树干注射施药技术研究概况[J].农药学学报,2005,7(2):104-108.WANG H Y,ZHAO Y A,JIA Y J.Trunk injection techniques with chemicals[J].Chin J Pestic Sci,2005,7(2):104-108.
[10]董崭,袁会珠,翟明利,等.树干注射吡虫啉、印楝素和乙酰甲胺磷防治槐尺蠖的效果比较[J].植物保护,2008,34(4):145-148.DONG C,YUAN H Z,ZHAI M L,et al.The control efficiencies of imidacloprid,azadirachtin and acephate against Sermothisa cinerearia were compared by trunk injection[J].Plant Prot,2008,34(4):145-148.
[11]PENG Z K,ZHENG H X,XIE W,et al.Field resistance monitoring of the immature stages of the whitefly Bemisia tabaci to spirotetramat in China[J].Crop Prot,2017,98:243-247.
[12]HE J T,ZHOU L J,YAO Q,et al.Greenhouse and field-based studies on the distribution of dimethoate in cotton and its effect on Tetranychus urticae by drip irrigation[J].Pest Manag Sci,2018,74(1):225-233.
[13]BAI D L,LUMMIS S C R,LEICHT W,et al.Actions of imidacloprid and a related nitromethylene on cholinergic receptors of an identified insect motor neurone[J].Pestic Sci,1991,33(2):197-204.
[14]BYRNE F J,URENA A A,ROBINSON L J,et al.Evaluation of neonicotinoid,organophosphate and avermectin trunk injections for the management of avocado thrips in California avocado groves[J].Pest Manag Sci,2012,68(5):811-817.
[15]DEMBILIó,RIBA J M,GAMóN M,et al.Mobility and efficacy of abamectin and imidacloprid against Rhynchophorus ferrugineus in Phoenix canariensis by different application methods.[J].Pest Manag Sci,2015,71(8):1091-1098.
[16]农药田间药效试验准则(二):GB/T 17980[S].北京:中国标准出版社,2004.Pesticide guidelines for the field efficacy trials(Ⅱ):GB/T 17980[S].Beingjing:Standards Press of China,2004.
[17]农药残留试验准则:NY/T 788-2004[S].北京:中国农业出版社,2004.Guideline on pesticide residue trials:NY/T 788-2004[S].Beijing:China Agriculture Press,2004.
[18]MOHAPATRA S,DEEPA M,LEKHA S,et al.Residue dynamics of spirotetramat and imidacloprid in/on mango and soil[J].Bull Environ Contam Toxicol,2012,89(4):862-867.
[19]MOHAPATRA S,KUMAR S,PRAKASH G S.Residue evaluation of imidacloprid,spirotetramat,and spirotetramat-enol in/on grapes(Vitis vinifera L.)and soil[J].Environ Monit Assess,2015,187(10):1-12.
[20]食品安全国家标准食品中农药最大残留限量:GB 2763-2014[S].北京:中国农业出版社,2014.National food safety standard,maximum residue limits for pesticides in food:GB 2763-2014[S].Beijing:China agriculture press,2014.
[21]MARCIC D,PETRONIJEVIC S,DROBNJAKOVIC T,et al.The effects of spirotetramat on life history traits and population growth of Tetranychus urticae(Acari:Tetranychidae)[J].Exp Appl Acarol,2012,56(2):113-122.
[22]尹可锁,杨韶松,郑泗军,等.吡虫啉不同施药方式防控香蕉蓟马的研究[J].中国南方果树,2017,46(2):111-113.YIN K S,YANG S S,ZHENG S J,et al.Study on the control efficacy of imidacloprid aganist banana thrips by different application methods[J].South China fruits,2017,46(2):111-113.
[23]尹可锁,郑泗军,杨韶松,等.利用假茎注射吡虫啉防控香蕉蓟马[J].植物保护,2016,42(5):238-241.YIN K S,ZHENG S J,YANG S S,et al.Control of thrips by injecting imidacloprid into banana pseudostem[J].Plant Prot,2016,42(5):238-241.
[24]丁丹,杨仁斌,雷鸣,等.螺虫乙酯及其四种代谢产物在柑桔中的消解残留规律研究[J].中国南方果树,2018,47(3):41-45.DING D,YANG R B,LEI M,et al.Study on degradation and residual regulation of spirotetramat and its 4 metabolites in citrus[J].South China Fruits,2018,47(3):41-45.
[25]吴育佳,杨仁斌,聂红英.螺虫乙酯在黄瓜及其土壤中残留量检测方法及残留规律研究[J].中国农学通报,2015,31(35):110-115.WU Y J,YANG R B,NIE H Y.Study on residual detection method and residue rule of spirotetramat in cucumber and soil[J].Chin Agric Sci Bull,2015,31(35):110-115.
[26]刘冰洁,贾春虹,陈莉,等.QuEChERS-超高效液相色谱-串联质谱法测定吡虫啉在花椰菜和土壤中的残留[J].食品安全质量检测学报,2018,9(6):1254-1260.LIU B J,JIA C H,CHEN L,et al.Determination of imidacloprid residues in cauliflower and soil by QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry[J].J Food Saf Qual,2018,9(6):1254-1260.
[27]FAO.Pesticides residues in food 2008.Joint FAO/WHO Meeting on Pesticide Residues[J].FAO Plant Production&Protection Paper,2009.
[28]黄玉南,乔成奎,庞涛,等.螺虫乙酯及其代谢产物在猕猴桃中的残留消解动态[J].现代食品科技,2018,34(12):221-227.HUANG Y N,QIAO C K,PANG T,et al.Residue and dissipation of spirotetramat and its metabolites in kiwifruits and soil[J].Modern Food Science and Technology,2018,34(12):221-227.
[29]徐诚,张圣虎,张毅,等.超高效液相色谱-三重四极杆-离子阱质谱法检测柑橘中螺虫乙酯及其4种代谢产物[J].色谱,2018,36(4):339-344.XU C,ZHANG S H,ZHANG Y,et al.Determination of spirotetramat and its four metabolites in citrus by ultra-high performance liquid chromatographytriple quadrupole-ion trap mass spectrometry[J].Chin J Chromatogr,2018,36(4):339-344.
[30]范银君,史雪岩,高希武.新烟碱类杀虫剂吡虫啉和噻虫嗪的代谢研究进展[J].农药学学报,2012,14(6):587-596.FAN Y J,SHI X Y,GAO X W.Research progresses on the metabolism of neonicotinoids imidacloprid and thiamethoxam[J].Chin J Pestic Sci,2012,14(6):587-596.
[2]MURAI T.Development and reproductive capacity of Thrips hawaiiensis(Thysanoptera:Thripidae)and its potential as a major pest[J].Bull Entomol Res,2001,91(3):193-198.
[3]付步礼,李强,夏西亚,等.对乙基多杀菌素中度抗性降低黄胸蓟马的适合度[J].昆虫学报,2017,60(2):180-188.FU B L,LI Q,XIA X Y,et al.Moderate resistance to spinetoram reduces the fitness of Thrips hawaiiensis(Thysanoptera:Thripidae)[J].Acta Entomologica Sinica,2017,60(2):180-188.
[4]付步礼,唐良德,邱海燕,等.黄胸蓟马高效低毒防治新型药剂的筛选[J].果树学报,2016,33(4):473-481.FU B L,TANG L D,QIU H Y,et al.Screening of high effect and low toxicity insecticides for controlling Thrips hawaiiensis(Morgan)[J].JFruit Sci,2016,33(4):473-481.
[5]李强,付步礼,夏西亚,等.溴氰虫酰胺对香蕉田间黄胸蓟马的药效及其残留规律[J].农药学学报,2017,19(6):723-728.LI Q,FU B L,XIA X Y,et al.Efficiency of cyantraniliprole on Thrips hawaiiensis and its residue behaviour in banana field[J].Chin JPestic Sci,2017,19(6):723-728.
[6]MEYERDIRK D E,FRENCH J V,HART W G.Citrus mealybug:effect of pesticide residues on adults of the natural enemy complex[J].J Econ Entomol,1979,72(6):893-895.
[7]KOHJIMOTO T,NAGAOKA H,TAKAGI Y,et al.Side-effects of pesticide residues on strawberry leaves against natural enemies[J].Annu Rep Kanto-Tosan Plant Prot Soc,2004,51:145-149.
[8]KUMAR A R,RAHMAN V J,KUMAR D V,et al.Utilization of the bacterium,Pseudomonas putida as a potential biocontrol agent against red spider mite,Oligonychus coffeae(Acari:Tetranychidae)infesting tea[J].J Plantat Crops,2011,39(1):236-238.
[9]王慧瑜,赵玉安,贾耀军.树干注射施药技术研究概况[J].农药学学报,2005,7(2):104-108.WANG H Y,ZHAO Y A,JIA Y J.Trunk injection techniques with chemicals[J].Chin J Pestic Sci,2005,7(2):104-108.
[10]董崭,袁会珠,翟明利,等.树干注射吡虫啉、印楝素和乙酰甲胺磷防治槐尺蠖的效果比较[J].植物保护,2008,34(4):145-148.DONG C,YUAN H Z,ZHAI M L,et al.The control efficiencies of imidacloprid,azadirachtin and acephate against Sermothisa cinerearia were compared by trunk injection[J].Plant Prot,2008,34(4):145-148.
[11]PENG Z K,ZHENG H X,XIE W,et al.Field resistance monitoring of the immature stages of the whitefly Bemisia tabaci to spirotetramat in China[J].Crop Prot,2017,98:243-247.
[12]HE J T,ZHOU L J,YAO Q,et al.Greenhouse and field-based studies on the distribution of dimethoate in cotton and its effect on Tetranychus urticae by drip irrigation[J].Pest Manag Sci,2018,74(1):225-233.
[13]BAI D L,LUMMIS S C R,LEICHT W,et al.Actions of imidacloprid and a related nitromethylene on cholinergic receptors of an identified insect motor neurone[J].Pestic Sci,1991,33(2):197-204.
[14]BYRNE F J,URENA A A,ROBINSON L J,et al.Evaluation of neonicotinoid,organophosphate and avermectin trunk injections for the management of avocado thrips in California avocado groves[J].Pest Manag Sci,2012,68(5):811-817.
[15]DEMBILIó,RIBA J M,GAMóN M,et al.Mobility and efficacy of abamectin and imidacloprid against Rhynchophorus ferrugineus in Phoenix canariensis by different application methods.[J].Pest Manag Sci,2015,71(8):1091-1098.
[16]农药田间药效试验准则(二):GB/T 17980[S].北京:中国标准出版社,2004.Pesticide guidelines for the field efficacy trials(Ⅱ):GB/T 17980[S].Beingjing:Standards Press of China,2004.
[17]农药残留试验准则:NY/T 788-2004[S].北京:中国农业出版社,2004.Guideline on pesticide residue trials:NY/T 788-2004[S].Beijing:China Agriculture Press,2004.
[18]MOHAPATRA S,DEEPA M,LEKHA S,et al.Residue dynamics of spirotetramat and imidacloprid in/on mango and soil[J].Bull Environ Contam Toxicol,2012,89(4):862-867.
[19]MOHAPATRA S,KUMAR S,PRAKASH G S.Residue evaluation of imidacloprid,spirotetramat,and spirotetramat-enol in/on grapes(Vitis vinifera L.)and soil[J].Environ Monit Assess,2015,187(10):1-12.
[20]食品安全国家标准食品中农药最大残留限量:GB 2763-2014[S].北京:中国农业出版社,2014.National food safety standard,maximum residue limits for pesticides in food:GB 2763-2014[S].Beijing:China agriculture press,2014.
[21]MARCIC D,PETRONIJEVIC S,DROBNJAKOVIC T,et al.The effects of spirotetramat on life history traits and population growth of Tetranychus urticae(Acari:Tetranychidae)[J].Exp Appl Acarol,2012,56(2):113-122.
[22]尹可锁,杨韶松,郑泗军,等.吡虫啉不同施药方式防控香蕉蓟马的研究[J].中国南方果树,2017,46(2):111-113.YIN K S,YANG S S,ZHENG S J,et al.Study on the control efficacy of imidacloprid aganist banana thrips by different application methods[J].South China fruits,2017,46(2):111-113.
[23]尹可锁,郑泗军,杨韶松,等.利用假茎注射吡虫啉防控香蕉蓟马[J].植物保护,2016,42(5):238-241.YIN K S,ZHENG S J,YANG S S,et al.Control of thrips by injecting imidacloprid into banana pseudostem[J].Plant Prot,2016,42(5):238-241.
[24]丁丹,杨仁斌,雷鸣,等.螺虫乙酯及其四种代谢产物在柑桔中的消解残留规律研究[J].中国南方果树,2018,47(3):41-45.DING D,YANG R B,LEI M,et al.Study on degradation and residual regulation of spirotetramat and its 4 metabolites in citrus[J].South China Fruits,2018,47(3):41-45.
[25]吴育佳,杨仁斌,聂红英.螺虫乙酯在黄瓜及其土壤中残留量检测方法及残留规律研究[J].中国农学通报,2015,31(35):110-115.WU Y J,YANG R B,NIE H Y.Study on residual detection method and residue rule of spirotetramat in cucumber and soil[J].Chin Agric Sci Bull,2015,31(35):110-115.
[26]刘冰洁,贾春虹,陈莉,等.QuEChERS-超高效液相色谱-串联质谱法测定吡虫啉在花椰菜和土壤中的残留[J].食品安全质量检测学报,2018,9(6):1254-1260.LIU B J,JIA C H,CHEN L,et al.Determination of imidacloprid residues in cauliflower and soil by QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry[J].J Food Saf Qual,2018,9(6):1254-1260.
[27]FAO.Pesticides residues in food 2008.Joint FAO/WHO Meeting on Pesticide Residues[J].FAO Plant Production&Protection Paper,2009.
[28]黄玉南,乔成奎,庞涛,等.螺虫乙酯及其代谢产物在猕猴桃中的残留消解动态[J].现代食品科技,2018,34(12):221-227.HUANG Y N,QIAO C K,PANG T,et al.Residue and dissipation of spirotetramat and its metabolites in kiwifruits and soil[J].Modern Food Science and Technology,2018,34(12):221-227.
[29]徐诚,张圣虎,张毅,等.超高效液相色谱-三重四极杆-离子阱质谱法检测柑橘中螺虫乙酯及其4种代谢产物[J].色谱,2018,36(4):339-344.XU C,ZHANG S H,ZHANG Y,et al.Determination of spirotetramat and its four metabolites in citrus by ultra-high performance liquid chromatographytriple quadrupole-ion trap mass spectrometry[J].Chin J Chromatogr,2018,36(4):339-344.
[30]范银君,史雪岩,高希武.新烟碱类杀虫剂吡虫啉和噻虫嗪的代谢研究进展[J].农药学学报,2012,14(6):587-596.FAN Y J,SHI X Y,GAO X W.Research progresses on the metabolism of neonicotinoids imidacloprid and thiamethoxam[J].Chin J Pestic Sci,2012,14(6):587-596.
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