拟微绿球藻生长对4种重金属的去除效果研究
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摘要
微藻作为生物吸附剂吸附重金属元素具有环保、高效及易解吸附的优点。采用拟微绿球藻(Nannochloropsis XJ006)作为重金属吸附剂,研究了不同浓度的重金属Pb~(2+)、Cd~(2+)、Hg~(2+)、As~(3+)对拟微绿球藻生长的影响以及拟微绿球藻对不同浓度4种重金属的去除率。结果表明拟微绿球藻在Pd~(2+)浓度0～9 mg/L、Cd~(2+)浓度0～5 mg/L、Hg~(2+)浓度0～0. 15 mg/L、As~(3+)浓度0～2.5 mg/L范围内可以正常生长,拟微绿球藻对Cd~(2+)的去除率最高达88.40%,Pd~(2+)、Hg~(2+)为80%,As~(3+)为40.04%。通过正交试验表明,Pb~(2+) 5 mg/L、Cd~(2+) 1mg/L、Hg~(2+) 0.05 mg/L及As~(3+)0.5mg/L时,拟微绿球藻生长状况最佳。
As a biosorbent adsorption of heavy metal elements, microalgae have the following advantages of environmental protection,efficient and easy to dissolve. In this paper,Nannochloropsis XJ006 was used as the heavy metal adsorbent. The effects of different concentrations of heavy metals Pb~(2+), Cd~(2+),Hg~(2+) and As~(3+) on Nannochloropsis XJ006 growth, and the removal rate of four heavy metals in different concentrations were studied. The results showed that when the Pd~(2+), Cd~(2+), Hg~(2+) and As~(3+) was at the concentration rang of 0-9 mg/L, 0-5 mg/L, 0-0. 15 mg/L and 0-2. 5 mg/L, respectively, Nannochloropsis XJ006 can grow normally. The removal rate of Cd~(2+) was 88.40%, Pd~(2+), Hg~(2+) was 80% and As~(3+) was 40.04%.The orthogonal experiment showed that under the concentration of Pd~(2+) 5 mg/L, Cd~(2+) 1 mg/L,Hg~(2+) 0. 05 mg/L and As~(3+) 0. 5 mg/L, the algae grows best.
As a biosorbent adsorption of heavy metal elements, microalgae have the following advantages of environmental protection,efficient and easy to dissolve. In this paper,Nannochloropsis XJ006 was used as the heavy metal adsorbent. The effects of different concentrations of heavy metals Pb~(2+), Cd~(2+),Hg~(2+) and As~(3+) on Nannochloropsis XJ006 growth, and the removal rate of four heavy metals in different concentrations were studied. The results showed that when the Pd~(2+), Cd~(2+), Hg~(2+) and As~(3+) was at the concentration rang of 0-9 mg/L, 0-5 mg/L, 0-0. 15 mg/L and 0-2. 5 mg/L, respectively, Nannochloropsis XJ006 can grow normally. The removal rate of Cd~(2+) was 88.40%, Pd~(2+), Hg~(2+) was 80% and As~(3+) was 40.04%.The orthogonal experiment showed that under the concentration of Pd~(2+) 5 mg/L, Cd~(2+) 1 mg/L,Hg~(2+) 0. 05 mg/L and As~(3+) 0. 5 mg/L, the algae grows best.
引文
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[6]林海,陈思,董颖博,等.黑藻、狐尾藻对重金属铅、镉、铬、钒污染水体的修复[J].中国有色金属学报,2017,21(7):178-186.
[7]FOLGAR S,TORRES E,PEREZ-RAMA M,et al. Dunaliella salina as marine microalga highly tolerant to but a poor remover of cadmium[J]. Journal of Hazardous Materials,2009,165(1/2/3):486-493.
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[9]战玉杰,杨茹君,王修林,等. Hg(Ⅱ)和Pb(Ⅱ)对海洋单细胞藻的急性毒性效应[J].生态毒理学报,2011,6(5):523-531.
[10]SMEDLEY P L,KINNIBURGH D G. A review of the source,behaviour and distribution of arsenic in natural waters[J]. Applied Geochemistry,2002,17(5):517-568.
[11]MONTEIRO C M,CASTRO P M L,MALCATA F X. Metal uptake by microalgae:underlying mechanisms and practical applications[J]. Biotechnology Progress,2012,28(2):299-311.
[2]NRIAGU J O. A silent epidemic of environmental metal poisoning?[J].Environmental Pollution,1988(1-2),50:139-161.
[3]刘慧君,龚仁敏,张小平,等.极大螺旋藻(Spirulina maxima)对六种重金属离子的生物吸附作用[J].安徽师范大学学报(自然科学版),2004,27(1):68-70.
[4]杨秀艳.一株微绿球藻的分离鉴定及p H对微藻脂肪酸的影响[D].青岛:中国海洋大学,2013.
[5]刘璐,闫浩,李诚,等.重金属铅与两种淡水藻的相互作用[J].生态学报,2014,34(7):1690-1697.
[6]林海,陈思,董颖博,等.黑藻、狐尾藻对重金属铅、镉、铬、钒污染水体的修复[J].中国有色金属学报,2017,21(7):178-186.
[7]FOLGAR S,TORRES E,PEREZ-RAMA M,et al. Dunaliella salina as marine microalga highly tolerant to but a poor remover of cadmium[J]. Journal of Hazardous Materials,2009,165(1/2/3):486-493.
[8]李坤,李琳,王福强,等.淡水小球藻清除Cu2+、Cd2+、Zn2+污染能力的研究[J].中国微生态学杂志,2006,18(3):194-195,198.
[9]战玉杰,杨茹君,王修林,等. Hg(Ⅱ)和Pb(Ⅱ)对海洋单细胞藻的急性毒性效应[J].生态毒理学报,2011,6(5):523-531.
[10]SMEDLEY P L,KINNIBURGH D G. A review of the source,behaviour and distribution of arsenic in natural waters[J]. Applied Geochemistry,2002,17(5):517-568.
[11]MONTEIRO C M,CASTRO P M L,MALCATA F X. Metal uptake by microalgae:underlying mechanisms and practical applications[J]. Biotechnology Progress,2012,28(2):299-311.