藏鸡源致病性大肠杆菌的分离鉴定及常用抗生素敏感性试验研究
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摘要
为了解藏鸡源致病性大肠杆菌的流行情况并摸索出适合的分离鉴定方法,试验采用细菌分离纯化培养、生化试验、16S rRNA序列鉴定的方法对从西藏林芝市西藏农牧学院临床兽医大楼散养藏鸡中采集的37份粪样中分离到的8株革兰氏阴性杆菌进行研究。结果表明:本次试验所分离到的8株革兰氏阴性杆菌为大肠杆菌,本试验分离到的8株大肠杆菌的生化特性基本保持一致,16S rRNA序列的鉴定也显示符合大肠杆菌的特性,测序比对发现分离株与产毒性大肠杆菌同源性达到99%以上。说明西藏地区林芝市藏鸡群中有大肠杆菌病分布且8株分离菌株是5类常见的致病性大肠杆菌中肠产毒性大肠杆菌的一种,具有一定的致病性,提示我们在临床中要正确用药,加强本地区的饲养管理。
In order to understand the prevalence of pathogenic Escherichia coli from Tibetan chickens and explore suitable separation and identification methods, the methods of bacterial isolation and purification culture, biochemical test and 16 S rRNA sequence identification were used in this experiment to study the 37 collectted samples from the scattered Tibetan Chicken of the Clinical Veterinary Building of Tibet Agriculture and Animal Husbandry College in Linzhi of Tibet, and 8 strains of Gram-negative bacilli were isolated from fecal samples. The results showed that the 8 strains of Gram-negative bacilli isolated from this experiment were Escherichia coli. The biochemical characteristics of 8 strains of Escherichia coli isolated in this experiment were basically same, and the identification of the 16 S rRNA sequence also showed that it conformed to the characteristics of Escherichia coli. The sequencing alignment showed that the isolate had a homology of more than 99% with the toxigenic Escherichia coli. This paper indicated that there was Escherichia coli distribution in Tibetan chicken flocks in Linzhi of Tibet, and 8 isolates were a common type of pathogenic Escherichia coli,and it had certain pathogenicity, which suggetted that we should use drugs correctly in the clinic to strengthen the management of the area.
In order to understand the prevalence of pathogenic Escherichia coli from Tibetan chickens and explore suitable separation and identification methods, the methods of bacterial isolation and purification culture, biochemical test and 16 S rRNA sequence identification were used in this experiment to study the 37 collectted samples from the scattered Tibetan Chicken of the Clinical Veterinary Building of Tibet Agriculture and Animal Husbandry College in Linzhi of Tibet, and 8 strains of Gram-negative bacilli were isolated from fecal samples. The results showed that the 8 strains of Gram-negative bacilli isolated from this experiment were Escherichia coli. The biochemical characteristics of 8 strains of Escherichia coli isolated in this experiment were basically same, and the identification of the 16 S rRNA sequence also showed that it conformed to the characteristics of Escherichia coli. The sequencing alignment showed that the isolate had a homology of more than 99% with the toxigenic Escherichia coli. This paper indicated that there was Escherichia coli distribution in Tibetan chicken flocks in Linzhi of Tibet, and 8 isolates were a common type of pathogenic Escherichia coli,and it had certain pathogenicity, which suggetted that we should use drugs correctly in the clinic to strengthen the management of the area.
引文
[1]林大捷.鸡大肠杆菌分离鉴定与药敏试验研究[J].黑龙江畜牧兽医,2015(8):108-110.
[2]张春梅.一例雏鸭大肠杆菌病的诊治[J].畜牧兽医科技信息,2013(2):100-101.
[3]张利会.一例蛋鸡大肠杆菌病的诊治[J].现代农村科技,2010(8):36-36.
[4]黄丽.鸡大肠杆菌病的防控[J].兽医导刊,2017(5):32-33.
[5]张东.鸡新城疫并发大肠杆菌病的诊治[J].当代畜禽养殖业, 2017(3):38-39.
[6]王剑春,何国安.以皮肤出血、坏死为明显表现的鸡传染病鉴别诊断[J].兽医导刊,2014(6):150-151.
[7]师福山,赵德明.禽大肠杆菌的分离与16S rRNA的鉴定[J].中国畜牧兽医,2009,36(2);111-113.
[8]冯方周,张晓冬,安倩等.辽西地区致病性鸡大肠杆菌的分离鉴定及16S rRNA序列分析[J].黑龙江畜牧兽医,2017(15):191-195.
[9]阎冰.红树林土壤细菌和古菌的16S rDNA多样性研究[D].武汉:华中农业大学,2007.
[10]白蓝.转基因香石竹遗传稳定性及其对土壤微生物群落影响初探[D].南京:南京农业大学,2010.
[11]律海峡.鸡源大肠杆菌外排基因acrA的m RNA表达水平测定[D].郑州:河南农业大学,2010.
[12]胡建和,赵坤,王天有等.地方性猪大肠埃希氏菌多价灭活苗的制备及应用[J].河南科技学院学报(自然科学版),2000,28(1):31-34.
[13]Angiuoli SV,Gussman A,Klimke W,et al.2008.Toward anonline repository of standard operating procedures(SOPs)for(meta)genomic annotation[J].Omics,2008,12:137–141.
[14]Kaper JB, Nataro JP, Mobley HLT.Pathogenic Escherichia coli[J].Nat Rev Microbiol,2004,2:123-140.
[15]王刚,罗润波,贡嘎等.西藏牦牛源大肠杆菌毒力基因检测与分型[J].中国兽医学报,2017,37(10):1913-1918.
[16]Elliott SJ,Wainwright LA,Timothy K,Jarvis KG,Deng Y,Lai L,Mcnamara BP,Michael S,Kaper JB:The complete sequence of the locus of enterocyte effacement(LEE)from enteropathogenic Escherichia coli E2348/69[J].Mol Microbiol,1998,28:1-4.
[17]Mellies JL,Barron AMS,Carmona AM:Enteropathogenic and enterohemorrhagic Escherichia coli virulence gene regulation[J].Infect Immun,2007,75:4199-4210.
[18]占思远,董尧,李利等.山羊IGFBP-2基因的克隆,序列分析及其组织表达[J].中国农业科学,2016,49(10):1198-2007.
[19]索朗斯珠,王刚,罗润波等.西藏牦牛源大肠杆菌主要耐药表型及耐药基因检测[J].中国兽医学报,2017,37(8):1501-1506.
[20]童晓梅.藏鸡线粒体全基因组序列的测定和分析[J].遗传,2006,28(7):769-777.
[2]张春梅.一例雏鸭大肠杆菌病的诊治[J].畜牧兽医科技信息,2013(2):100-101.
[3]张利会.一例蛋鸡大肠杆菌病的诊治[J].现代农村科技,2010(8):36-36.
[4]黄丽.鸡大肠杆菌病的防控[J].兽医导刊,2017(5):32-33.
[5]张东.鸡新城疫并发大肠杆菌病的诊治[J].当代畜禽养殖业, 2017(3):38-39.
[6]王剑春,何国安.以皮肤出血、坏死为明显表现的鸡传染病鉴别诊断[J].兽医导刊,2014(6):150-151.
[7]师福山,赵德明.禽大肠杆菌的分离与16S rRNA的鉴定[J].中国畜牧兽医,2009,36(2);111-113.
[8]冯方周,张晓冬,安倩等.辽西地区致病性鸡大肠杆菌的分离鉴定及16S rRNA序列分析[J].黑龙江畜牧兽医,2017(15):191-195.
[9]阎冰.红树林土壤细菌和古菌的16S rDNA多样性研究[D].武汉:华中农业大学,2007.
[10]白蓝.转基因香石竹遗传稳定性及其对土壤微生物群落影响初探[D].南京:南京农业大学,2010.
[11]律海峡.鸡源大肠杆菌外排基因acrA的m RNA表达水平测定[D].郑州:河南农业大学,2010.
[12]胡建和,赵坤,王天有等.地方性猪大肠埃希氏菌多价灭活苗的制备及应用[J].河南科技学院学报(自然科学版),2000,28(1):31-34.
[13]Angiuoli SV,Gussman A,Klimke W,et al.2008.Toward anonline repository of standard operating procedures(SOPs)for(meta)genomic annotation[J].Omics,2008,12:137–141.
[14]Kaper JB, Nataro JP, Mobley HLT.Pathogenic Escherichia coli[J].Nat Rev Microbiol,2004,2:123-140.
[15]王刚,罗润波,贡嘎等.西藏牦牛源大肠杆菌毒力基因检测与分型[J].中国兽医学报,2017,37(10):1913-1918.
[16]Elliott SJ,Wainwright LA,Timothy K,Jarvis KG,Deng Y,Lai L,Mcnamara BP,Michael S,Kaper JB:The complete sequence of the locus of enterocyte effacement(LEE)from enteropathogenic Escherichia coli E2348/69[J].Mol Microbiol,1998,28:1-4.
[17]Mellies JL,Barron AMS,Carmona AM:Enteropathogenic and enterohemorrhagic Escherichia coli virulence gene regulation[J].Infect Immun,2007,75:4199-4210.
[18]占思远,董尧,李利等.山羊IGFBP-2基因的克隆,序列分析及其组织表达[J].中国农业科学,2016,49(10):1198-2007.
[19]索朗斯珠,王刚,罗润波等.西藏牦牛源大肠杆菌主要耐药表型及耐药基因检测[J].中国兽医学报,2017,37(8):1501-1506.
[20]童晓梅.藏鸡线粒体全基因组序列的测定和分析[J].遗传,2006,28(7):769-777.
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