牛樟芝菌种培养基质筛选

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英文篇名：Screening culture medium of Taiwanofungus camphorates 作者：蒋忠成 ; 郑元 ; 张颖 ; 杨宇明 ; 王娟 ; 陈翔 英文作者：Jiang Zhongcheng;Zheng Yuan;Zhang Ying;Yang Yuming;Wang Juan;Chen Xiang;College of Forest, Southwest Forestry University;Key Laboratory of Forest Disaster Warning and Control in Yunnan Province, Southwest Forestry University;Yunnan Academy of Forestry;Southwest Green Development Research Institute, Southwest Forestry University;Forestry Science and Technology Test Center in Fujian; 关键词：牛樟芝 ; 氮源 ; 碳源 ; 种源 ; 筛选 英文关键词：Taiwanofungus camphoratus;;the best source;;strain screening 中文刊名：食药用菌 英文刊名：Edible and Medicinal Mushrooms 机构：西南林业大学林学院;西南林业大学云南省森林灾害预警与控制重点实验室;云南省林业科学院;西南林业大学西南绿色发展研究院;福建省林业科技试验中心; 出版日期：2019-05-19 出版单位：食药用菌 年：2019 期：03 基金：云南省科技计划项目“牛樟树、牛樟芝优质种质资源及其培育关键技术引进”(2015IA004);; 西南林业大学科研项目“牛樟树、牛樟芝重要功效成分生理代谢”(2176062);; 云南省博士后定向培养资助 语种：中文; 页：57-62 页数：6 CN：33-1371/S ISSN：2095-0934 分类号：S567.3

摘要

采用固体恒温暗培养的方式,通过改变培养基的营养基质,探究牛樟芝菌丝体对不同碳氮源的效应。结果为:蛋白胨和果糖是菌丝生长的最适氮源和碳源,最优的种源是2号,3、5和6号次之。不同碳氮源分别在10～15天、15～20天平均生长速度最快。对比发现,牛樟芝菌丝体在葡萄糖、胡萝卜基质上生长最快的是10～15天。在胡萝卜、果糖基质上的菌落颜色最鲜艳。
        In this research, different nitrogen and carbon sources were used to culture mycelium of Taiwanofungus camphoratus on solid constant temperature condition, for explore the effects of mediums on the growth of mycelium,and the growth status of the mycelium were regularly observed. The results showed that the optimal nitrogen and carbon sources for the growth of mycelium of Taiwanofungus camphoratus were peptones and fructose. The best strain is No. 2,No. 3, 5 and 6 came second. The average maximum growth rate of different carbon and nitrogen sources were 10～15 d or 15～20 d after inoculation, respectively. By comparing the average growth rate of each nitrogen and carbon source at different periods, it was found that mycelium grew fastest in the culture medium with carrot extract as the nitrogen source and glucose as the carbon source. When carrot extract was taken as nitrogen source and fructose as carbon source,the colony color was redder, which was the closest to the wild fruitbody color of Taiwanofungus camphoratus. The results can be used to estimate the growth rate of Taiwanofungus camphoratus, strains expanding propagation, liquid fermentation and wood inoculation.

引文

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