高效液相色谱法同时测定通络驻景丸中金丝桃苷和毛蕊花糖苷两种成分
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摘要
目的探讨采用高效液相色谱法(HPLC)同时测定通络驻景丸中的金丝桃苷和毛蕊花糖苷两种有效成分。方法使用Agilent ZORBAX SB-C18(4.6 mm×250 mm×5μm)作为色谱柱,以乙腈和0.1%的醋酸溶液作为流动相,梯度洗脱,流动相的流速为1 mL/min,检测波长分别为360 nm和334 nm。结果通络驻景丸中金丝桃苷和毛蕊花糖苷两种有效成分的峰面积与其进样量有较好的线性关系,并且它们的相关系数均超过0.999 3,所使用仪器精密度的标准差不超过2%,平均回收率均大于95%。结论采用高效液相色谱法测定通络驻景丸中金丝桃苷和毛蕊花糖苷这两种有效成分,快速、简便、回收率高,是测定通络驻景丸中这两种标志性有效成分的有效方法。
Objective To explore the use of high performance liquid chromatography(HPLC) to determine the two active ingredients of Hyperoside and Verbascoside in Tongluo Zhujing Pills.Methods Agilent ZORBAX SBC18(4.6 mm×250 mm×5 μ m) was used as chromatographic column,acetonitrile and 0.1% acetic acid solution as mobile phase,gradient elution.The flow rate of mobile phase was 1 mL/min,detection wavelength was 360 nm and 334 nm respectively.Results The sample volume and peak area of two active ingredients of Hyperoside and Verbascoside in Tongluo Zhujing Pills has a good linear relationship.Their correlation coefficients exceeded 0.999 3.The standard deviation of the precision of the instrument used does not exceed 2% and the average recovery rate was greater than 95%.Conclusion The method of HPLC determination of two active ingredients of Hyperoside and Verbascoside in Tongluo Zhujing Pills is high-speed,easy,high recovery and this method is very effective.
Objective To explore the use of high performance liquid chromatography(HPLC) to determine the two active ingredients of Hyperoside and Verbascoside in Tongluo Zhujing Pills.Methods Agilent ZORBAX SBC18(4.6 mm×250 mm×5 μ m) was used as chromatographic column,acetonitrile and 0.1% acetic acid solution as mobile phase,gradient elution.The flow rate of mobile phase was 1 mL/min,detection wavelength was 360 nm and 334 nm respectively.Results The sample volume and peak area of two active ingredients of Hyperoside and Verbascoside in Tongluo Zhujing Pills has a good linear relationship.Their correlation coefficients exceeded 0.999 3.The standard deviation of the precision of the instrument used does not exceed 2% and the average recovery rate was greater than 95%.Conclusion The method of HPLC determination of two active ingredients of Hyperoside and Verbascoside in Tongluo Zhujing Pills is high-speed,easy,high recovery and this method is very effective.
引文
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[4]刘甲平,尚荣国,李高彪,等.通络驻景丸的质量标准研究[J].中医药临床杂志,2017,29(10):1681-1685.
[5]刘莹.驻景丸、加减驻景丸、驻景丸加减方的溯源及其在眼科的应用[J].西部中医药,2010,23(5):55-56.
[6]刘甲平,尚荣国,李高彪,等.通络驻景丸的质量标准研究[J].中医药临床杂志,2017,29(10):1681-1685.
[7]朱宇红,尚荣国,郝武常,等.通络驻景丸的质量控制研究[J].陕西中医药大学学报,2018,41(1):97-99,107.
[8]王克年.驻景丸方剂治疗原发性视网膜色素变性的疗效观察[J].中国民康医学,2018,30(5):87,90.
[9]李乃谦.熟地黄活性成分药理作用的研究进展[J].中国处方药,2017,15(1):14-15.
[10]徐莲,吴岚,王雪,等.黄芪、何首乌、女贞子、菟丝子混合提取物对体外培养毛囊生长的影响及药理作用研究[J].现代生物医学进展,2014,14(22):4201-4204.
[11]雷蒙蒙.浅析车前子新药理作用[J].亚太传统医药,2017,13(15):57-58.
[12]陈娟,倪军,王艳艳.三七药理作用的研究进展[J].双足与保健,2017,26(19):186-187.
[13]焦增华,杨亚军,刘希望,等.蒲黄药理作用研究进展[J].中兽医医药杂志,2017,36(3):85-88.
[14]程敏,邓雅婷,王庆伟.墨旱莲有效成分的提取工艺与药理作用研究进展[J].中国药师,2015,18(11):1956-1959.
[15]王丹彤,王丹辉.中药地龙的化学成分及药理作用研究[J].世界最新医学信息文摘,2015,15(68):254-255.
[16]黄崇才.砂仁的化学成分、药理作用及临床应用的研究进展[J].内蒙古中医药,2017,36(21/22):210-212.
[17]刘欢,杨婧,雷晓琴,等.通络驻景丸对糖尿病视网膜病变的临床疗效[J].中国中医眼科杂志,2015,25(6):392-395.
[18]陈彦霓,陈子林.抗VEGF药物治疗新生血管性眼病的现状与展望[J].医学综述,2016,22(8):1550-1553.
[19]雷晓琴,杨婧,刘欢,等.通络驻景丸对糖尿病视网膜病变代谢记忆的抗氧化机理研究[J].中国现代医药杂志,2015,17(11):4-6.
[20]李伟,郭伟,肖芳喜,等.温通散眼部热熨对早期糖尿病视网膜病变眼血流及视网膜内层血液循环状态的影响[J].吉林中医药,2017,37(11):1108-1110.
[21]刘月仙.影响玻璃体视网膜术后早期高眼压的因素[J].长春中医药大学学报,2015,31(3):618-619.