燕麦种子萌发期抗旱能力的综合评价
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摘要
为了研究不同燕麦材料的抗旱能力,以83份燕麦为试验对象,以200 g/L聚乙二醇(PEG-6000)进行胁迫处理,测定各燕麦品种的相对发芽势、相对发芽率、发芽指数、种子萌发率、发芽势和发芽率共6个形态指标,通过计算并绘制韦恩图,筛选出9份抗旱性较强的燕麦材料。进一步对9份燕麦萌发期的丙二醛(MDA)质量摩尔浓度、脯氨酸(Pro)质量分数、过氧化物酶(POD)活性及超氧化物歧化酶(SOD)活性进行测定。在主成分分析的基础上,利用加权隶属函数法构建燕麦萌发期抗旱性的综合评价体系,探究燕麦种子萌发的抗旱评价指标。通过两种指标同时检测和相互映证,建立萌发期燕麦抗旱综合评价体系,研究结果表明‘2010-48’和‘H44’为强抗旱品种;‘2010-5’和‘04-111’为中抗旱品种;‘ly05-2’‘05-22’‘06-31’‘2010-26’‘05-34’为弱抗旱品种。
To study the drought resistance of different oat germplasms, the grains of 83 naked oats treated with 200 g/L PEG-6000 were used to determine 6 morphological indexes, including the relative germination potential, relative germination rate, germination index, seed germination rate, germination potential and germination rate, and then the Venn Diagram was made and used to evaluate drought resistance of oats during germination stage. Further more, nine oats with drought-resistant were treated with 200 g/L PEG-6000 for checking malondialdehyde(MDA), proline(PRO) content, peroxidase(POD) activity and superoxide dismutase(SOD) activity. Whereafter, the drought resistance index of germinated seeds was evaluated according to the principal component analysis and weighted function method. Based on evaluation of the morphological index and physiological indexes, a comprehensive evaluation system for drought resistance of oats was conducted. The results indicated that the drought resistance of ‘2010-48' and ‘H44' are the strongest; the materials ‘2010-5' and ‘04-111' rank middle, the ‘ly05-2'‘05-22'‘06-31'‘2010-26' and ‘05-34' the worst among all of samples.
To study the drought resistance of different oat germplasms, the grains of 83 naked oats treated with 200 g/L PEG-6000 were used to determine 6 morphological indexes, including the relative germination potential, relative germination rate, germination index, seed germination rate, germination potential and germination rate, and then the Venn Diagram was made and used to evaluate drought resistance of oats during germination stage. Further more, nine oats with drought-resistant were treated with 200 g/L PEG-6000 for checking malondialdehyde(MDA), proline(PRO) content, peroxidase(POD) activity and superoxide dismutase(SOD) activity. Whereafter, the drought resistance index of germinated seeds was evaluated according to the principal component analysis and weighted function method. Based on evaluation of the morphological index and physiological indexes, a comprehensive evaluation system for drought resistance of oats was conducted. The results indicated that the drought resistance of ‘2010-48' and ‘H44' are the strongest; the materials ‘2010-5' and ‘04-111' rank middle, the ‘ly05-2'‘05-22'‘06-31'‘2010-26' and ‘05-34' the worst among all of samples.
引文
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[14]闫江艳,张永清,冯晓敏,等.干旱胁迫及复水对不同黍稷品种根系生理特性的影响[J].西北植物学报,2012,32(2):348-354.YAN J Y,ZHANG Y Q,FENG X M,et al.Effect of drought stress and rewatering on physiological characteristics of roots in different proso millet varieties[J].Acta Botanica Boreali-occidentalia Sinica,2012,32(2):348-354.
[15]黄国宾,张晓海,杨双龙,等.渗透调节参与循环干旱锻炼提高烟草植物抗旱性的形成[J].植物生理学报,2012,48(5):465-471.HUANG G B,ZHANG X H,YANG SH L,et al.Involvement of osmotic regulation in enhancement of drought resistance in tobacco(Nicotiana tabacum L.)plants through circular drought-hardening[J].Plant Physiology Journal,2012,48(5):465-471.
[16]王琰,陈建文,狄晓艳.水分胁迫下不同油松种源SOD、POD、MDA及可溶性蛋白比较研究[J].生态环境学报,2011,20(10):1449-1453.WANG Y,CHEN J W,DI X Y.A comparative study on the SOD,POD,MDA and dissoluble protein of six provenances of Chinese pine(Pinus tabulaeformis Carr.)under water stress[J].Ecology&Environmental Sciences,2011,20(10):1449-1453.
[17]GRACE S C.Plant phenolic antioxidant and prooxidant activities:phenolics-induced oxidative damage mediated by metals in plants[J].Toxicology,2002,177(1):67-80.
[18]杨旭东,聂慧,侯建华,等.PEG模拟干旱胁迫对向日葵种子萌发的影响[J].种子,2016,35(5):71-75.YANG X D,NIE H,HOU J H,et al.Effect of polyethylene glycol(PEG)simulated drought stress on seed germination of sunflower[J].Seed,2016,35(5):71-75.
[19]邓胜兴,曾明,熊伟,等.干旱胁迫对柑橘叶片保护酶系统的影响[J].西南大学学报(自然科学版),2009,31(2):61-64.DENG SH X,ZENG M,XIONG W,et al.Effects of drought stress on the antioxidative enzyme system in citrus leaves[J].Journal of Southwest University(Natural Science Edition),2009,31(2):61-64.
[20]曲涛,南志标.作物和牧草对干旱胁迫的响应及机理研究进展[J].草业学报,2008,7(2):126-135.QU T,NAN ZH B.Research progress on responses and mechanisms of crop and grass under drought stress[J].Acta Prataculturae Sinica,2008,7(2):126-135.
[21]田梦雨.干旱胁迫对小麦苗期生长的影响及其生理机制[D].南京:南京农业大学,2009.TIAN M Y.Effect of drought stresses on seedling growth and its physiological mechanism in wheat[D].Nanjing:Nanjing Agricultural University,2009.
[22]全国农作物种子标准化技术委员会.GB/T 3543.4-1995农作物种子检验规程发芽试验[S].北京:中国标准出版社,1995.National Technical Committee for Crop Seed Standardization.GB/T 3543.4-1995 Rules for agricultural seed testing-germination test[S].Beijing:Standards Press of China,1995.
[23]张霞,谢小玉.PEG胁迫下甘蓝型油菜种子萌发期抗旱鉴定指标的研究[J].西北农业学报,2012,21(2):72-77.ZHANG X,XIE X Y.Studies on identification indexes of drought resistance by peg during seed germination of rapeseed(Brassica napus L.)[J].Acta Agriculturae Boreali-occidentalis Sinica,2012,21(2):72-77.
[24]贾利强.金沙江热河谷造林树种抗旱特性的研究[D].北京:北京林业大学,2003.JIA L Q.Studies on drought resistance character of silvicultural species in Jinsha River[D].Beijing:Beijing Forestry University,2003.
[25]SHARMA P,DUBEY R S.Drought-induced oxidative stress and enhances the activities of antioxidant enzymes in growing rice seedlings[J].Plant Growth Regulation,2005,46(3):209-221.
[26]张博文,李富平,许永利,等.PEG-6000模拟干旱胁迫下5种草本植物的抗旱性[J].分子植物育种,2018,16(8):2686-2695.ZHANG B W,LI F P,XU Y L,et al.Drought resistance of five herbs under drought stress simulated by PEG-6000[J].Molecular Plant Breeding,2018,16(8):2686-2695.
[2]谢小玉,张霞,张兵,等.油菜苗期抗旱性评价及抗旱相关指标变化分析[J].中国农业科学,2013,46(3):476-485.XIE X Y,ZHANG X,ZHANG B.Evaluation of drought resistance and analysis of variation of relevant parameters at seeding stage of rapeseed(Brassica Napus L.)[J].Scientia Agricultura Sinic,2013,46(3):476-485.
[3]STEVENS E J,ARMSTRONG K W,BEZAR H J,et al.Fodder oats:an overview[J].Fodder Oats a World Overview,2004:1-9.
[4]BLUM A,EBERCON A.Cell membrane stability as a measure of drought and heat tolerance in wheat[J].Crop Science,1981,21(1):43-47.
[5]TRIPATHY J N,ZHANG J,ROBIN S,et al.QTLs for cellmembrane stability mapped in rice(Oryza sativa L.)under drought stress[J].Theoretical and Applied Genetics,2000,100(8):1197-1202.
[6]PREMACHANDRA G S,SANEOKA H,OGATA S.Evaluation of polyethylene glycol test for measuring cell membrane stability in maize[J].Soil Science&Plant Nutrition,2010,35(4):565-573.
[7]张明生,谈锋,张启堂.快速鉴定甘薯品种抗旱性的生理指标及方法的筛选[J].中国农业科学,2001,34(3):260-265.ZHANG M SH,TAN F,ZHANG Q T.Physiological indices for rapid identification of sweet potato drought resistance and selection of methods[J].Scientia Agricultura Sinica,2001,34(3):260-265.
[8]路阳,金山,张娜,等.西藏11种野生牧草萌发期抗旱性研究[J].西北农业学报,2011,20(3):38-44.LU Y,JIN SH,ZHANG N,et al.Drought resistance evaluation of 11wild grasses from Tibet during germination[J].Acta Agriculturae Boreali-occidentalis Sinica,2011,20(3):38-44.
[9]WANG J G,CHEN G C,ZHANG C L.The effects of water stress on soluble protein content,the activity of SOD,PODand CAT of two ecotypes of reeds(Phragmites communis)[J].Acta Botanica Boreali-occidentalia Sinica,2002,22(3):561-565.
[10]OLIVARESVILLEGAS J J,REYNOLDS M P,MCDO-NALD G K.Drought-adaptive attributes in the Seri/Babaxhexaploid wheat population[J].Functional Plant Biology,2007,34(3):189-203.
[11]GAO W,ZHANG Y,FENG Z,et al.Effects of melatonin on antioxidant capacity in naked oat seedlings under drought stress[J].Molecules,2018,23(7):1580.
[12]WANG T,DU Y L,HE J,et al.Recently-released genotypes of naked oat(Avena nuda L.)out-yield early releases under water-limited conditions by greater reproductive allocation and desiccation tolerance[J].Field Crops Research,2017,204:169-179.
[13]ROHAMARE Y,DHUMAL K N,NIKAM T D.Response of Ajowan to water stress induced by polyethylene glycol(PEG)6000during seed germination and seedling growth[J].Journal of Environmental Biology,2014,35(5):789-793.
[14]闫江艳,张永清,冯晓敏,等.干旱胁迫及复水对不同黍稷品种根系生理特性的影响[J].西北植物学报,2012,32(2):348-354.YAN J Y,ZHANG Y Q,FENG X M,et al.Effect of drought stress and rewatering on physiological characteristics of roots in different proso millet varieties[J].Acta Botanica Boreali-occidentalia Sinica,2012,32(2):348-354.
[15]黄国宾,张晓海,杨双龙,等.渗透调节参与循环干旱锻炼提高烟草植物抗旱性的形成[J].植物生理学报,2012,48(5):465-471.HUANG G B,ZHANG X H,YANG SH L,et al.Involvement of osmotic regulation in enhancement of drought resistance in tobacco(Nicotiana tabacum L.)plants through circular drought-hardening[J].Plant Physiology Journal,2012,48(5):465-471.
[16]王琰,陈建文,狄晓艳.水分胁迫下不同油松种源SOD、POD、MDA及可溶性蛋白比较研究[J].生态环境学报,2011,20(10):1449-1453.WANG Y,CHEN J W,DI X Y.A comparative study on the SOD,POD,MDA and dissoluble protein of six provenances of Chinese pine(Pinus tabulaeformis Carr.)under water stress[J].Ecology&Environmental Sciences,2011,20(10):1449-1453.
[17]GRACE S C.Plant phenolic antioxidant and prooxidant activities:phenolics-induced oxidative damage mediated by metals in plants[J].Toxicology,2002,177(1):67-80.
[18]杨旭东,聂慧,侯建华,等.PEG模拟干旱胁迫对向日葵种子萌发的影响[J].种子,2016,35(5):71-75.YANG X D,NIE H,HOU J H,et al.Effect of polyethylene glycol(PEG)simulated drought stress on seed germination of sunflower[J].Seed,2016,35(5):71-75.
[19]邓胜兴,曾明,熊伟,等.干旱胁迫对柑橘叶片保护酶系统的影响[J].西南大学学报(自然科学版),2009,31(2):61-64.DENG SH X,ZENG M,XIONG W,et al.Effects of drought stress on the antioxidative enzyme system in citrus leaves[J].Journal of Southwest University(Natural Science Edition),2009,31(2):61-64.
[20]曲涛,南志标.作物和牧草对干旱胁迫的响应及机理研究进展[J].草业学报,2008,7(2):126-135.QU T,NAN ZH B.Research progress on responses and mechanisms of crop and grass under drought stress[J].Acta Prataculturae Sinica,2008,7(2):126-135.
[21]田梦雨.干旱胁迫对小麦苗期生长的影响及其生理机制[D].南京:南京农业大学,2009.TIAN M Y.Effect of drought stresses on seedling growth and its physiological mechanism in wheat[D].Nanjing:Nanjing Agricultural University,2009.
[22]全国农作物种子标准化技术委员会.GB/T 3543.4-1995农作物种子检验规程发芽试验[S].北京:中国标准出版社,1995.National Technical Committee for Crop Seed Standardization.GB/T 3543.4-1995 Rules for agricultural seed testing-germination test[S].Beijing:Standards Press of China,1995.
[23]张霞,谢小玉.PEG胁迫下甘蓝型油菜种子萌发期抗旱鉴定指标的研究[J].西北农业学报,2012,21(2):72-77.ZHANG X,XIE X Y.Studies on identification indexes of drought resistance by peg during seed germination of rapeseed(Brassica napus L.)[J].Acta Agriculturae Boreali-occidentalis Sinica,2012,21(2):72-77.
[24]贾利强.金沙江热河谷造林树种抗旱特性的研究[D].北京:北京林业大学,2003.JIA L Q.Studies on drought resistance character of silvicultural species in Jinsha River[D].Beijing:Beijing Forestry University,2003.
[25]SHARMA P,DUBEY R S.Drought-induced oxidative stress and enhances the activities of antioxidant enzymes in growing rice seedlings[J].Plant Growth Regulation,2005,46(3):209-221.
[26]张博文,李富平,许永利,等.PEG-6000模拟干旱胁迫下5种草本植物的抗旱性[J].分子植物育种,2018,16(8):2686-2695.ZHANG B W,LI F P,XU Y L,et al.Drought resistance of five herbs under drought stress simulated by PEG-6000[J].Molecular Plant Breeding,2018,16(8):2686-2695.