木薯‘新选048’自交系群体表型鉴定评价及遗传多样性分析
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摘要
为了研究木薯‘新选048’自交系群体的遗传差异,本研究通过变异系数、遗传多样性指数、多态性比例、遗传相似系数、聚类分析等方面对‘新选048’的83个自交系株系进行表型鉴定及遗传多样性分析。结果表明,自交系株系各性状的变异系数处于9.94%~157.74%之间,数量性状的变异系数低于质量性状,叶长最低,茎分叉习性最高;遗传多样性指数处于0.07~1.91之间,数量性状的遗传多样性指数高于质量性状,叶尖形状和顶端叶颜色最低,最大薯长最高。块根中氢氰酸(HCN)、淀粉和可溶性总糖含量均发生分离。与亲本相比,各株系的淀粉含量均低,可溶性总糖含量均高,且分离出低HCN株系。相关性分析表明,极显著相关的性状有26对,显著相关的性状有16对。24个表型性状共提取了9个主成分, 21个性状起主要作用。利用SSR分子标记进行遗传多样性分析, 18对引物共扩增出78条条带,多态性比例达89.74%,各株系间遗传相似系数介于0.40~0.90之间。对比表型聚类和分子标记聚类结果,发现二者具有一定的关联性,但不完全相同,聚类结果并无明显规律。本研究通过自交系鉴定出一批木薯中间材料,丰富了我国木薯种质资源的遗传多样性,并为木薯自交系育种提供参考。
In order to study the genetic differences of cassava ‘Xinxuan 048' inbred lines, the phenotypic identification and genetic diversity analysis of 83 ‘Xinxuan 048' inbred lines were carried out through variation coefficient, genetic diversity index, polymorphism ratio, genetic similarity coefficient and cluster analysis. The results showed that, variation coefficient of all relevant traits of the inbred lines ranged from 9.94% to 157.74%, the leaf length was the lowest, the stem fork habit was the highest, and variation coefficient of quantitative characters was lower than that of qualitative characters. The genetic diversity index was between 0.07 and 1.91, the genetic diversity index of quantitative characters was higher than that of qualitative characters. The genetic diversity index of the top leaf color and the tip shape was the lowest, the genetic diversity of the maximum tuber length was the largest. The phenotype value of hydrocyanic acid(HCN), starch and total soluble sugar content in the tuber of the population presented wide segregation. Compared with the original parent, starch content decreased, total soluble sugar content increased, and lines with low HCN content appeared. The correlation analysis showed that there were 26 pairs of highly significant correlated traits and 16 pairs of significantly correlated traits. Nine principal components were extracted from 24 phenotypic traits, and 21 traits played a key role. Totally, 78 bands were amplified by 18 pairs of primers, and the polymorphism ratio was 89.74%. The genetic similarity coefficients between the lines ranged from 0.40 to 0.90. By comparing the results of phenotypic clustering and molecular marker clustering, it was found that there was a certain correlation between them, but they were not identical. Meanwhile, there was no obvious rule in clustering results. In this study, a number of cassava intermediate materials were identified through inbred line, which enriched the genetic diversity of cassava germplasm resources in China and provided reference for cassava inbred line breeding.
In order to study the genetic differences of cassava ‘Xinxuan 048' inbred lines, the phenotypic identification and genetic diversity analysis of 83 ‘Xinxuan 048' inbred lines were carried out through variation coefficient, genetic diversity index, polymorphism ratio, genetic similarity coefficient and cluster analysis. The results showed that, variation coefficient of all relevant traits of the inbred lines ranged from 9.94% to 157.74%, the leaf length was the lowest, the stem fork habit was the highest, and variation coefficient of quantitative characters was lower than that of qualitative characters. The genetic diversity index was between 0.07 and 1.91, the genetic diversity index of quantitative characters was higher than that of qualitative characters. The genetic diversity index of the top leaf color and the tip shape was the lowest, the genetic diversity of the maximum tuber length was the largest. The phenotype value of hydrocyanic acid(HCN), starch and total soluble sugar content in the tuber of the population presented wide segregation. Compared with the original parent, starch content decreased, total soluble sugar content increased, and lines with low HCN content appeared. The correlation analysis showed that there were 26 pairs of highly significant correlated traits and 16 pairs of significantly correlated traits. Nine principal components were extracted from 24 phenotypic traits, and 21 traits played a key role. Totally, 78 bands were amplified by 18 pairs of primers, and the polymorphism ratio was 89.74%. The genetic similarity coefficients between the lines ranged from 0.40 to 0.90. By comparing the results of phenotypic clustering and molecular marker clustering, it was found that there was a certain correlation between them, but they were not identical. Meanwhile, there was no obvious rule in clustering results. In this study, a number of cassava intermediate materials were identified through inbred line, which enriched the genetic diversity of cassava germplasm resources in China and provided reference for cassava inbred line breeding.
引文
Chang LL,Wang LM,Guo AP,et al(2018).Differential phosphoproteomics of cassava leaves under drought stress.Plant Physiol J,54(1):133-144(in Chinese with English abstract)[常丽丽,王力敏,郭安平等(2018).木薯叶片响应干旱胁迫的磷酸化蛋白质组差异分析.植物生理学报,54(1):133-144]
Chen HX,Cao S,Yan HB,et al(2019).The effect of increasing bio-organic fertilizer on the yield and quality of edible-cassava.Chin J Trop Crop,40(3):417-424(in Chinese with English abstract)[陈会鲜,曹升,严华兵等(2019).增施生物有机肥对食用木薯产量及品质的影响.热带作物学报,40(3):417-424]
Dong HY,Sun WC,Liu ZG,et al(2014).Efffect of selfing on physiplogical and biochemical characteristics and cold resistance of winter turnip rape(Brassica campesteis L.).Acta Bot Boreal-Occident Sin,34(11):2277-2282(in Chinese with English abstract)[董红业,孙万仓,刘自刚等(2014).自交对白菜型冬油菜生理生化特性及抗寒性的影响.西北植物学报,34(11):2277-2282]
El-Sharkawy MA(2004).Cassava biology and physiology.Plant Mol Biol,56(4):481-501
Gu B,Yao QQ,Li KM,et al(2013).Change in physicochemical traits of cassava roots and starches associated with genotypes and environmental factors.Starch-St?rke,65(3-4):253-263
Guo Q(2016).Inheritance and variation study of sugar and acid content in the hybrid and selfing offsprings fruit in Vitis amurensis Rupr(dissertation).Shenyang:Shenyang Agriculture University(in Chinese with English abstract)[郭权(2016).山葡萄杂交及自交后代果实糖酸含量遗传变异研究(学位论文).沈阳:沈阳农业大学]
Guo Y,Xiong HP,Chen P,et al(2013).Analysis on genetic diversity of S1 progeny of ramie variety Zhongzhu No.2by SSR markers.Plant Fiber Sci Chin,35(2):75-80(in Chinese with English abstract)[郭阳,熊和平,陈平等(2013).中苎2号苎麻自交S1代遗传多样性的SSR标记分析.中国麻业科学,35(2):75-80]
Guo YY,Zhou SM,Chen ZD,et al(2018).Identification,evaluation and genetic diversity analysisof local cucumber varieties from Guangxi.J South Agric,49(7):1273-1281(in Chinese with English abstract)[郭元元,周生茂,陈振东等(2018).广西黄瓜地方品种鉴定评价及遗传多样性分析.南方农业学报,49(7):1273-1281]
Juan PXF,Rafael PD,Saulo ASO,et al(2017).Inbreeding depression for severity caused by leaf diseases in cassava.Euphytica,213:205
Kawuki RS,Nuwamanya E,Labuschagne M,et al(2011).Segregation of selected agronomic traits in six S1 cassava families.J Plant Breed Crop Sci,3(8):154-160
Li J,Huang Q,Tian YN(2009).Breeding on cassava variety GR3 and its cultivation techniques.Guangxi Agric Sci,40(9):1147-1149(in Chinese with English abstract)[李军,黄强,田益农等(2009).木薯品种桂热3号的选育及栽培技术.广西农业科学,40(9):1147-1149]
Li KM,Lin X,Huang J,et al(2002).Selection and breeding of Manihot esculenta Crantz.cv.M.SC6.Chin J Trop Crop,23(4):39-43(in Chinese with English abstract)[李开绵,林雄,黄洁等(2002).华南6号木薯的选育.热带作物学报,23(4):39-43]
Luo XL(2009).Report on applying and breeding of new cassava cultivar Xinxuan 048.Chin Agri Bull,25(24):501-505(in Chinese with English abstract)[罗兴录(2009).木薯新品种新选048选育与应用.中国农学通报,25(24):501-505]
Shang XH,Shan ZY,Yan HB,et al(2018a).Physiological responses of cassava variety‘Xinxuan 048’diploid and autotetraploid to drought stress.Plant Physiol J,54(6):1064-1072(in Chinese with English abstract)[尚小红,单忠英,严华兵等(2018a).木薯‘新选048’二倍体及其同源四倍体对干旱胁迫的生理响应.植物生理学报,54(6):1064-1072]
Shang XH,Yan HB,Cao S,et al(2018b).Optimization of SCoT-PCR reaction system and primer selection for Pueraria DC.J South Agric,49(1):1-7(in Chinese with English abstract)[尚小红,严华兵,曹升等(2018b).葛根SCoT-PCR反应体系优化及引物筛选.南方农业学报,49(1):1-7]
Shang XH,Yan HB,Cao S,et al(2019).Genetic diversity analysis of Pueraria in Guangxi based on SCoT markers.J Nucl Agric Sci,33(7):1311-1317(in Chinese with English abstract)[尚小红,严华兵,曹升等(2019).广西地方葛根种质资源遗传多样性的SCoT分析.核农学报,33(7):1311-1317]
Tadeo K,Vincent K,Yona B,et al(2016).Inbreeding enhances field resistance to cassava brown streak viruses.J Plant Breed Crop Sci,8(8):138-149
Tan LM,Fu HY,Xu J,et al(2019).Identification of lemon zygotic self-crossed seedlings and the primary evaluation of the resistance to citrus canker disease.Mol Plant Breed,http://kns.cnki.net/kcms/detail/46.1068.S.20190507.1138.004.html(in Chinese with English abstract)[谭李梅,符红艳,徐静等(2019).柠檬合子胚自交实生苗的鉴定及其对柑橘溃疡病的抗性初步评价.分子植物育种,http://kns.cnki.net/kcms/detail/46.1068.S.20190507.1138.004.html]
Wang M,Xiao XH,An FF,et al(2015).Genetic diversity of cassava germplasm revealed by SSR markers.Chin JTrop Agric,35(11):38-44(in Chinese with English abstract)[王明,肖鑫辉,安飞飞等(2015).利用SSR标记分析木薯遗传多样性.热带农业科学,35(11):38-44]
Xiao L,Zhou HW,Xie XY,et al(2018).Analysis of genetic variation and DNA methylation diversity in diploid and autotetraploid of cassava.Chin J Trop Crop,39(5):833-839(in Chinese with English abstract)[肖亮,周慧文,谢向誉等(2018).木薯及其同源四倍体基因组与DNA甲基化差异分析.热带作物学报,39(5):833-839]
Xie XY,Lu LY,Zeng WD,et al(2017).Identification,evaluation and genetic diversity analysis of 31 cassava germplasm resources.J South Agric,48(3):393-400(in Chinese with English abstract)[谢向誉,陆柳英,曾文丹等(2017).31份木薯种质资源的鉴定评价及遗传多样性分析.南方农业学报,48(3):393-400]
Yan HB,Ye JQ,Li KM(2015).Progress of cassava breeding in China.Chin Agric Bull,31(15):63-70(in Chinese with English abstract)[严华兵,叶剑秋,李开绵(2015).中国木薯育种研究进展.中国农学通报,31(15):63-70]
Ye JQ,Li KM,Chen LZ,et al(2007).Breeding and selection of new cassava variety SC 7.Chin J Trop Crop,28(1):24-29(in Chinese with English abstract)[叶剑秋,李开绵,陈丽珍等(2007).木薯新品种华南7号的选育.热带作物学报,28(1):24-29]
Ye JQ,Zhou JG,Xue MF(2011).Cassava Germplasm Resources Map Form.Beijing:China Agriculture Press(in Chinese)[叶剑秋,周建国,薛茂富(2011).木薯种质资源形态图谱.北京:中国农业出报社]
Zeng WD,Yan HB,Cao S,et al(2018).A simple,rapid and accurate method for detecting HCN content in cassava:CN201810212391.1.2018-09-04[曾文丹,严华兵,曹升等(2018).一种木薯HCN含量简易快速准确的检测方法:CN201810212391.1.2018-09-04]
Zhao NJ,Meng QL,Zhang YW,et al(2015).Analysis of 44samples of genetic diversity of maize inhred lines using SSR.Seed,34(11):26-30(in Chinese with English abstract)[赵宁娟,孟庆立,张宇文等(2015).利用SSR标记分析44份玉米自交系的遗传多样性.种子,34(11):26-30]
Zhou HW,Xiao L,Zeng WD,et al(2019).In vitro induction and identification of autotetraploid cassava.J Nucl Agric Sci,33(4):647-652(in Chinese with English abstract)[周慧文,肖亮,曾文丹等(2019).木薯同源四倍体的离体诱导及鉴定.核农学报,33(4):647-652]
Zhou HW,Zeng WD,Yan HB(2017).In vitro induction of tetraploids in cassava variety‘Xinxuan 048’using colchicine.Plant Cell Tiss Organ Cult,128:723-729
Zou JX,Li KM,Wang WQ(2005).Evaluation on microsatellite molecular genetic diversity of cassava germplasm resources by SSR marker.J South China Univ Trop Agric,11(2):1-5(in Chinese with English abstract)[邹积鑫,李开绵,王文泉(2005).微卫星分子标记在木薯种质资源遗传分析中的应用.华南热带农业大学学报,11(2):1-5]
Chen HX,Cao S,Yan HB,et al(2019).The effect of increasing bio-organic fertilizer on the yield and quality of edible-cassava.Chin J Trop Crop,40(3):417-424(in Chinese with English abstract)[陈会鲜,曹升,严华兵等(2019).增施生物有机肥对食用木薯产量及品质的影响.热带作物学报,40(3):417-424]
Dong HY,Sun WC,Liu ZG,et al(2014).Efffect of selfing on physiplogical and biochemical characteristics and cold resistance of winter turnip rape(Brassica campesteis L.).Acta Bot Boreal-Occident Sin,34(11):2277-2282(in Chinese with English abstract)[董红业,孙万仓,刘自刚等(2014).自交对白菜型冬油菜生理生化特性及抗寒性的影响.西北植物学报,34(11):2277-2282]
El-Sharkawy MA(2004).Cassava biology and physiology.Plant Mol Biol,56(4):481-501
Gu B,Yao QQ,Li KM,et al(2013).Change in physicochemical traits of cassava roots and starches associated with genotypes and environmental factors.Starch-St?rke,65(3-4):253-263
Guo Q(2016).Inheritance and variation study of sugar and acid content in the hybrid and selfing offsprings fruit in Vitis amurensis Rupr(dissertation).Shenyang:Shenyang Agriculture University(in Chinese with English abstract)[郭权(2016).山葡萄杂交及自交后代果实糖酸含量遗传变异研究(学位论文).沈阳:沈阳农业大学]
Guo Y,Xiong HP,Chen P,et al(2013).Analysis on genetic diversity of S1 progeny of ramie variety Zhongzhu No.2by SSR markers.Plant Fiber Sci Chin,35(2):75-80(in Chinese with English abstract)[郭阳,熊和平,陈平等(2013).中苎2号苎麻自交S1代遗传多样性的SSR标记分析.中国麻业科学,35(2):75-80]
Guo YY,Zhou SM,Chen ZD,et al(2018).Identification,evaluation and genetic diversity analysisof local cucumber varieties from Guangxi.J South Agric,49(7):1273-1281(in Chinese with English abstract)[郭元元,周生茂,陈振东等(2018).广西黄瓜地方品种鉴定评价及遗传多样性分析.南方农业学报,49(7):1273-1281]
Juan PXF,Rafael PD,Saulo ASO,et al(2017).Inbreeding depression for severity caused by leaf diseases in cassava.Euphytica,213:205
Kawuki RS,Nuwamanya E,Labuschagne M,et al(2011).Segregation of selected agronomic traits in six S1 cassava families.J Plant Breed Crop Sci,3(8):154-160
Li J,Huang Q,Tian YN(2009).Breeding on cassava variety GR3 and its cultivation techniques.Guangxi Agric Sci,40(9):1147-1149(in Chinese with English abstract)[李军,黄强,田益农等(2009).木薯品种桂热3号的选育及栽培技术.广西农业科学,40(9):1147-1149]
Li KM,Lin X,Huang J,et al(2002).Selection and breeding of Manihot esculenta Crantz.cv.M.SC6.Chin J Trop Crop,23(4):39-43(in Chinese with English abstract)[李开绵,林雄,黄洁等(2002).华南6号木薯的选育.热带作物学报,23(4):39-43]
Luo XL(2009).Report on applying and breeding of new cassava cultivar Xinxuan 048.Chin Agri Bull,25(24):501-505(in Chinese with English abstract)[罗兴录(2009).木薯新品种新选048选育与应用.中国农学通报,25(24):501-505]
Shang XH,Shan ZY,Yan HB,et al(2018a).Physiological responses of cassava variety‘Xinxuan 048’diploid and autotetraploid to drought stress.Plant Physiol J,54(6):1064-1072(in Chinese with English abstract)[尚小红,单忠英,严华兵等(2018a).木薯‘新选048’二倍体及其同源四倍体对干旱胁迫的生理响应.植物生理学报,54(6):1064-1072]
Shang XH,Yan HB,Cao S,et al(2018b).Optimization of SCoT-PCR reaction system and primer selection for Pueraria DC.J South Agric,49(1):1-7(in Chinese with English abstract)[尚小红,严华兵,曹升等(2018b).葛根SCoT-PCR反应体系优化及引物筛选.南方农业学报,49(1):1-7]
Shang XH,Yan HB,Cao S,et al(2019).Genetic diversity analysis of Pueraria in Guangxi based on SCoT markers.J Nucl Agric Sci,33(7):1311-1317(in Chinese with English abstract)[尚小红,严华兵,曹升等(2019).广西地方葛根种质资源遗传多样性的SCoT分析.核农学报,33(7):1311-1317]
Tadeo K,Vincent K,Yona B,et al(2016).Inbreeding enhances field resistance to cassava brown streak viruses.J Plant Breed Crop Sci,8(8):138-149
Tan LM,Fu HY,Xu J,et al(2019).Identification of lemon zygotic self-crossed seedlings and the primary evaluation of the resistance to citrus canker disease.Mol Plant Breed,http://kns.cnki.net/kcms/detail/46.1068.S.20190507.1138.004.html(in Chinese with English abstract)[谭李梅,符红艳,徐静等(2019).柠檬合子胚自交实生苗的鉴定及其对柑橘溃疡病的抗性初步评价.分子植物育种,http://kns.cnki.net/kcms/detail/46.1068.S.20190507.1138.004.html]
Wang M,Xiao XH,An FF,et al(2015).Genetic diversity of cassava germplasm revealed by SSR markers.Chin JTrop Agric,35(11):38-44(in Chinese with English abstract)[王明,肖鑫辉,安飞飞等(2015).利用SSR标记分析木薯遗传多样性.热带农业科学,35(11):38-44]
Xiao L,Zhou HW,Xie XY,et al(2018).Analysis of genetic variation and DNA methylation diversity in diploid and autotetraploid of cassava.Chin J Trop Crop,39(5):833-839(in Chinese with English abstract)[肖亮,周慧文,谢向誉等(2018).木薯及其同源四倍体基因组与DNA甲基化差异分析.热带作物学报,39(5):833-839]
Xie XY,Lu LY,Zeng WD,et al(2017).Identification,evaluation and genetic diversity analysis of 31 cassava germplasm resources.J South Agric,48(3):393-400(in Chinese with English abstract)[谢向誉,陆柳英,曾文丹等(2017).31份木薯种质资源的鉴定评价及遗传多样性分析.南方农业学报,48(3):393-400]
Yan HB,Ye JQ,Li KM(2015).Progress of cassava breeding in China.Chin Agric Bull,31(15):63-70(in Chinese with English abstract)[严华兵,叶剑秋,李开绵(2015).中国木薯育种研究进展.中国农学通报,31(15):63-70]
Ye JQ,Li KM,Chen LZ,et al(2007).Breeding and selection of new cassava variety SC 7.Chin J Trop Crop,28(1):24-29(in Chinese with English abstract)[叶剑秋,李开绵,陈丽珍等(2007).木薯新品种华南7号的选育.热带作物学报,28(1):24-29]
Ye JQ,Zhou JG,Xue MF(2011).Cassava Germplasm Resources Map Form.Beijing:China Agriculture Press(in Chinese)[叶剑秋,周建国,薛茂富(2011).木薯种质资源形态图谱.北京:中国农业出报社]
Zeng WD,Yan HB,Cao S,et al(2018).A simple,rapid and accurate method for detecting HCN content in cassava:CN201810212391.1.2018-09-04[曾文丹,严华兵,曹升等(2018).一种木薯HCN含量简易快速准确的检测方法:CN201810212391.1.2018-09-04]
Zhao NJ,Meng QL,Zhang YW,et al(2015).Analysis of 44samples of genetic diversity of maize inhred lines using SSR.Seed,34(11):26-30(in Chinese with English abstract)[赵宁娟,孟庆立,张宇文等(2015).利用SSR标记分析44份玉米自交系的遗传多样性.种子,34(11):26-30]
Zhou HW,Xiao L,Zeng WD,et al(2019).In vitro induction and identification of autotetraploid cassava.J Nucl Agric Sci,33(4):647-652(in Chinese with English abstract)[周慧文,肖亮,曾文丹等(2019).木薯同源四倍体的离体诱导及鉴定.核农学报,33(4):647-652]
Zhou HW,Zeng WD,Yan HB(2017).In vitro induction of tetraploids in cassava variety‘Xinxuan 048’using colchicine.Plant Cell Tiss Organ Cult,128:723-729
Zou JX,Li KM,Wang WQ(2005).Evaluation on microsatellite molecular genetic diversity of cassava germplasm resources by SSR marker.J South China Univ Trop Agric,11(2):1-5(in Chinese with English abstract)[邹积鑫,李开绵,王文泉(2005).微卫星分子标记在木薯种质资源遗传分析中的应用.华南热带农业大学学报,11(2):1-5]
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